Restriction map for
pFN21AE1960
(Circular) (Six-base) MAPSORT of: pFN21AE1960.seq Check: 7015 from: 1 to: 6271
pFN21AE1960 6271 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4081 278
Size: 53 83 186 936 2545 2468
Fragments arranged by size:
2545 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2607 3096 3614 1937
Size: 670 489 518 4594
Fragments arranged by size:
4594 670 518 489
Acc65I G'GTAC_C
Cuts at: 3592 3592
Size: 6271
AclI AA'CG_TT
Cuts at: 1563 4398 4771 1563
Size: 2835 373 3063
Fragments arranged by size:
3063 2835 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2915 3422 4334 5718 5986 1632
Size: 1283 507 912 1384 268 1917
Fragments arranged by size:
1917 1384 1283 912 507 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6074
AflIII A'CryG_T
Cuts at: 1203 5170 6147 1203
Size: 3967 977 1327
Fragments arranged by size:
3967 1327 977
AgeI A'CCGG_T
Cuts at: 5102 5102
Size: 6271
AhdI GACnn_n'nnGTC
Cuts at: 5000 5000
Size: 6271
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6271
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6271
AlwNI CAG_nnn'CTG
Cuts at: 3388 4014 5586 3388
Size: 626 1572 4073
Fragments arranged by size:
4073 1572 626
ApaLI G'TGCA_C
Cuts at: 2745 4328 5484 2745
Size: 1583 1156 3532
Fragments arranged by size:
3532 1583 1156
ApoI r'AATT_y
Cuts at: 1493 3580 3806 3958 6067 1493
Size: 2087 226 152 2109 1697
Fragments arranged by size:
2109 2087 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4825 160
Size: 872 3793 1606
Fragments arranged by size:
3793 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6271
AvaI C'yCGr_G
Cuts at: 1870 1943 3596 1870
Size: 73 1653 4545
Fragments arranged by size:
4545 1653 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2520 2553 2520
Size: 33 6238
BamHI G'GATC_C
Cuts at: 3601 3968 3601
Size: 367 5904
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3268 3592 5047 618
Size: 325 217 627 1481 324 1455 1842
Fragments arranged by size:
1842 1481 1455 627 325 324 217
BanII G_rGCy'C
Cuts at: 1391 3590 1391
Size: 2199 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2232 3432 928
Size: 971 333 1200 3767
Fragments arranged by size:
3767 1200 971 333
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3230 3264 4462 4496 1903
Size: 34 1293 34 1198 34 3678
Fragments arranged by size:
3678 1293 1198 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3355 4163 5373 3355
Size: 808 1210 4253
Fragments arranged by size:
4253 1210 808
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6271
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4882 136
Size: 107 122 71 631 605 3210 1525
Fragments arranged by size:
3210 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6266 6266
Size: 6271
BlpI GC'TnA_GC
Cuts at: 2173 3688 2173
Size: 1515 4756
Bme1580I G_kGCm'C
Cuts at: 1790 2749 3164 3552 4332 5488 1790
Size: 959 415 388 780 1156 2573
Fragments arranged by size:
2573 1156 959 780 415 388
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6271
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4002 4960 442
Size: 416 53 83 399 24 2585 958 1753
Fragments arranged by size:
2585 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6271
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2180 2534 2746 4931 1239
Size: 941 354 212 2185 2579
Fragments arranged by size:
2579 2185 941 354 212
Bpu10I CC'TnA_GC
Cuts at: 3261 3261
Size: 6271
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1998 2105 4396 5261 5559 1998
Size: 107 2291 865 298 2710
Fragments arranged by size:
2710 2291 865 298 107
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2764 4934 882
Size: 617 1265 2170 2219
Fragments arranged by size:
2219 2170 1265 617
BsaAI yAC'GTr
Cuts at: 493 1204 4062 493
Size: 711 2858 2702
Fragments arranged by size:
2858 2702 711
BsaBI GATnn'nnATC
Cuts at: 6265 6265
Size: 6271
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4078 4460 5095 275
Size: 53 83 186 936 2545 382 635 1451
Fragments arranged by size:
2545 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4704 5102 5376 5523 5934 4704
Size: 398 274 147 411 5041
Fragments arranged by size:
5041 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3122 3152 1178
Size: 30 165 30 1719 30 4297
Fragments arranged by size:
4297 1719 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2240 1186
Size: 1054 5217
BseYI C'CCAG_C
Cuts at: 3507 5474 5970 3507
Size: 1967 496 3808
Fragments arranged by size:
3808 1967 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5698
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3721 4482 5510 1540
Size: 275 186 1720 761 1028 2301
Fragments arranged by size:
2301 1720 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2749 4332 4417 5488 2749
Size: 1583 85 1071 3532
Fragments arranged by size:
3532 1583 1071 85
BsmI GAATG_Cn'
Cuts at: 3782 3875 3782
Size: 93 6178
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2749 3164 3552 3590 4332 4417 5488
Size: 399 959 415 388 38 742 85 1071
Cuts at: 5488 1391
Size: 2174
Fragments arranged by size:
2174 1071 959 742 415 399 388 85
38
BspEI T'CCGG_A
Cuts at: 5934 5934
Size: 6271
BspHI T'CATG_A
Cuts at: 4160 5890 4160
Size: 1730 4541
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2364 2705 3626 844
Size: 1520 341 921 3489
Fragments arranged by size:
3489 1520 921 341
BsrBI CCG'CTC
Cuts at: 1394 1823 4158 1394
Size: 429 2335 3507
Fragments arranged by size:
3507 2335 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4766 4940 65
Size: 1183 3518 174 1396
Fragments arranged by size:
3518 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4915 5102 1700
Size: 110 3105 187 2869
Fragments arranged by size:
3105 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2829 96
Size: 2733 3538
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6271
BssSI C'ACGA_G
Cuts at: 4331 5343 4331
Size: 1012 5259
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6271
BstBI TT'CG_AA
Cuts at: 5075 5075
Size: 6271
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6271
BstXI CCAn_nnnn'nTGG
Cuts at: 2954 2954
Size: 6271
BstYI r'GATC_y
Cuts at: 1972 2209 2928 3601 3968 4353 4370 5822 5908
Size: 237 719 673 367 385 17 1452 86
Cuts at: 5908 5920 6266 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 719 673 385 367 346 237
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3076 513
Size: 552 2011 3708
Fragments arranged by size:
3708 2011 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5532
Fragments arranged by size:
5532 683 56
BtsI GCAGTG_nn'
Cuts at: 3164 3444 3787 4581 4601 3164
Size: 280 343 794 20 4834
Fragments arranged by size:
4834 794 343 280 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6271
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6236
DraI TTT'AAA
Cuts at: 3575 3575
Size: 6271
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5278 809
Size: 507 3962 1802
Fragments arranged by size:
3962 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3324 3718 4607
Size: 54 1425 50 275 18 1494 394 889
Cuts at: 4607 6236 8
Size: 1629 43
Fragments arranged by size:
1629 1494 1425 889 394 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3718 1537
Size: 275 1906 4090
Fragments arranged by size:
4090 1906 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3106 3210 3466 4201 1353
Size: 1753 104 256 735 3423
Fragments arranged by size:
3423 1753 735 256 104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4858 5232 5378 387
Size: 1064 138 3269 374 146 1280
Fragments arranged by size:
3269 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2180 2534 2746 2915 3422 4334 4931
Size: 393 548 354 212 169 507 912 597
Cuts at: 4931 5718 5986 1239
Size: 787 268 1524
Fragments arranged by size:
1524 912 787 597 548 507 393 354
268 212 169
EcoO109I rG'GnC_Cy
Cuts at: 2518 3112 2518
Size: 594 5677
EcoRI G'AATT_C
Cuts at: 3580 3958 3580
Size: 378 5893
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2444 2689 2444
Size: 245 6026
FspI TGC'GCA
Cuts at: 4777 4777
Size: 6271
HaeII r_GCGC'y
Cuts at: 1678 5418 1678
Size: 3740 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2920 2952 3122 3124 3154
Size: 32 135 32 1348 32 170 2 30
Cuts at: 3154 3156 3419 3451 4912 4944 4986 5018 1373
Size: 2 263 32 1461 32 42 32 2626
Fragments arranged by size:
2626 1461 1348 263 170 135 42 32
32 32 32 32 32 30 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2021 2872 3454 3615 3861 669
Size: 988 281 83 851 582 161 246 3079
Fragments arranged by size:
3079 988 851 582 281 246 161 83
HindIII A'AGCT_T
Cuts at: 748 2072 748
Size: 1324 4947
HpaI GTT'AAC
Cuts at: 3861 3861
Size: 6271
KpnI G_GTAC'C
Cuts at: 3596 3596
Size: 6271
MfeI C'AATT_G
Cuts at: 3870 3870
Size: 6271
MluI A'CGCG_T
Cuts at: 6147 6147
Size: 6271
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5385 5569 1305
Size: 4080 184 2007
Fragments arranged by size:
4080 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3326 10
Size: 54 1425 343 1494 2955
Fragments arranged by size:
2955 1494 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2919 2979 4229 4588 4747 518
Size: 648 1753 60 1250 359 159 2042
Fragments arranged by size:
2042 1753 1250 648 359 159 60
MspA1I CmG'CkG
Cuts at: 1606 2825 3372 3687 4364 5512 5757 1606
Size: 1219 547 315 677 1148 245 2120
Fragments arranged by size:
2120 1219 1148 677 547 315 245
NcoI C'CATG_G
Cuts at: 513 1065 3076 513
Size: 552 2011 3708
Fragments arranged by size:
3708 2011 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6271
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6271
NotI GC'GGCC_GC
Cuts at: 3718 3718
Size: 6271
NspI r_CATG'y
Cuts at: 1132 1229 2511 2714 3359 3629 5174 1132
Size: 97 1282 203 645 270 1545 2229
Fragments arranged by size:
2229 1545 1282 645 270 203 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6271
PciI A'CATG_T
Cuts at: 5170 5170
Size: 6271
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2110 1108
Size: 1002 5269
PmeI GTTT'AAAC
Cuts at: 3575 3575
Size: 6271
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4340 4372 5853 5885 4340
Size: 32 1481 32 4726
Fragments arranged by size:
4726 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2518 2518
Size: 6271
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6271
PsiI TTA'TAA
Cuts at: 3841 3841
Size: 6271
PstI C_TGCA'G
Cuts at: 830 2283 3015 3159 3285 3623 830
Size: 1453 732 144 126 338 3478
Fragments arranged by size:
3478 1453 732 338 144 126
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6271
PvuII CAG'CTG
Cuts at: 2825 3372 2825
Size: 547 5724
SalI G'TCGA_C
Cuts at: 1936 3613 1936
Size: 1677 4594
SbfI CC_TGCA'GG
Cuts at: 3285 3623 3285
Size: 338 5933
ScaI AGT'ACT
Cuts at: 4519 6231 4519
Size: 1712 4559
SfcI C'TryA_G
Cuts at: 826 1045 2279 2604 3011 3155 3281 3619 4754
Size: 219 1234 325 407 144 126 338 1135
Cuts at: 4754 5435 5626 826
Size: 681 191 1471
Fragments arranged by size:
1471 1234 1135 681 407 338 325 219
191 144 126
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6271
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6271
SmaI CCC'GGG
Cuts at: 1872 3598 1872
Size: 1726 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2013 2120 4375 5276 5538 820
Size: 197 926 70 107 2255 901 262 1553
Fragments arranged by size:
2255 1553 926 901 262 197 107 70
SnaBI TAC'GTA
Cuts at: 493 4062 493
Size: 3569 2702
SpeI A'CTAG_T
Cuts at: 152 2644 3535 152
Size: 2492 891 2888
Fragments arranged by size:
2888 2492 891
SphI G_CATG'C
Cuts at: 1132 2714 3629 1132
Size: 1582 915 3774
Fragments arranged by size:
3774 1582 915
SspI AAT'ATT
Cuts at: 5 52 4195 5
Size: 47 4143 2081
Fragments arranged by size:
4143 2081 47
StuI AGG'CCT
Cuts at: 1517 2856 3203 1517
Size: 1339 347 4585
Fragments arranged by size:
4585 1339 347
StyI C'CwwG_G
Cuts at: 513 1065 3076 3208 3710 513
Size: 552 2011 132 502 3074
Fragments arranged by size:
3074 2011 552 502 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3286 4298 4315 4468 4653 5098 325
Size: 1255 1706 1012 17 153 185 445 1498
Fragments arranged by size:
1706 1498 1255 1012 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2829 4517 6229
Size: 274 80 33 51 1443 852 1688 1712
Cuts at: 6229 96
Size: 138
Fragments arranged by size:
1712 1688 1443 852 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6231 6263 6231
Size: 32 6239
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6271
XbaI T'CTAG_A
Cuts at: 2470 3607 2470
Size: 1137 5134
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2155 2305 3083 2155
Size: 150 778 5343
Fragments arranged by size:
5343 778 150
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6271
XmaI C'CCGG_G
Cuts at: 1870 3596 1870
Size: 1726 4545
XmnI GAAnn'nnTTC
Cuts at: 4400 4400
Size: 6271
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4079 276
Size: 53 83 186 936 2545 2468
Fragments arranged by size:
2545 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI