Restriction map for
pFN21AE1957
(Circular) (Six-base) MAPSORT of: pFN21AE1957.seq Check: 4234 from: 1 to: 6241
pFN21AE1957 6241 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2759 2759
Size: 6241
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4051 278
Size: 53 83 186 936 2515 2468
Fragments arranged by size:
2515 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3584 1937
Size: 1647 4594
Acc65I G'GTAC_C
Cuts at: 3562 3562
Size: 6241
AclI AA'CG_TT
Cuts at: 1563 4368 4741 1563
Size: 2805 373 3063
Fragments arranged by size:
3063 2805 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2399 2640 3032 3274 4304 5688 5956 1632
Size: 767 241 392 242 1030 1384 268 1917
Fragments arranged by size:
1917 1384 1030 767 392 268 242 241
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6044
AflIII A'CryG_T
Cuts at: 1203 2796 3078 5140 6117 1203
Size: 1593 282 2062 977 1327
Fragments arranged by size:
2062 1593 1327 977 282
AgeI A'CCGG_T
Cuts at: 5072 5072
Size: 6241
AhdI GACnn_n'nnGTC
Cuts at: 4970 4970
Size: 6241
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6241
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6241
AlwNI CAG_nnn'CTG
Cuts at: 2338 2389 3010 3184 3984 5556 2338
Size: 51 621 174 800 1572 3023
Fragments arranged by size:
3023 1572 800 621 174 51
ApaLI G'TGCA_C
Cuts at: 4298 5454 4298
Size: 1156 5085
ApoI r'AATT_y
Cuts at: 1493 2434 3550 3776 3928 6037 1493
Size: 941 1116 226 152 2109 1697
Fragments arranged by size:
2109 1697 1116 941 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4795 160
Size: 872 3763 1606
Fragments arranged by size:
3763 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6241
AvaI C'yCGr_G
Cuts at: 1870 1943 2600 3566 1870
Size: 73 657 966 4545
Fragments arranged by size:
4545 966 657 73
BamHI G'GATC_C
Cuts at: 3571 3938 3571
Size: 367 5874
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2201 2297 2486 3562 5017
Size: 325 217 627 414 96 189 1076 1455
Cuts at: 5017 618
Size: 1842
Fragments arranged by size:
1842 1455 1076 627 414 325 217 189
96
BanII G_rGCy'C
Cuts at: 1391 2215 2231 3335 3560 1391
Size: 824 16 1104 225 4072
Fragments arranged by size:
4072 1104 824 225 16
BbeI G_GCGC'C
Cuts at: 2205 2205
Size: 6241
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3280 928
Size: 971 1381 3889
Fragments arranged by size:
3889 1381 971
BbvCI CC'TCA_GC
Cuts at: 2614 2614
Size: 6241
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4432 4466 1903
Size: 34 2495 34 3678
Fragments arranged by size:
3678 2495 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4133 5343 4133
Size: 1210 5031
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6241
BfrBI ATG'CAT
Cuts at: 2888 2888
Size: 6241
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2658 4852 136
Size: 107 122 71 631 605 986 2194 1525
Fragments arranged by size:
2194 1525 986 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6236 6236
Size: 6241
BlpI GC'TnA_GC
Cuts at: 2114 3658 2114
Size: 1544 4697
Bme1580I G_kGCm'C
Cuts at: 1790 2491 4302 5458 1790
Size: 701 1811 1156 2573
Fragments arranged by size:
2573 1811 1156 701
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6241
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2485 3200 3523
Size: 416 53 83 399 24 1068 715 323
Cuts at: 3523 3972 4930 442
Size: 449 958 1753
Fragments arranged by size:
1753 1068 958 715 449 416 399 323
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6241
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2495 2495
Size: 6241
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2573 3206 3259 3443 3478 4901 1239
Size: 1334 633 53 184 35 1423 2579
Fragments arranged by size:
2579 1423 1334 633 184 53 35
Bpu10I CC'TnA_GC
Cuts at: 2614 2614
Size: 6241
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4366 5231 5529 4366
Size: 865 298 5078
Fragments arranged by size:
5078 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2183 4904 882
Size: 617 684 2721 2219
Fragments arranged by size:
2721 2219 684 617
BsaAI yAC'GTr
Cuts at: 493 1204 4032 493
Size: 711 2828 2702
Fragments arranged by size:
2828 2702 711
BsaBI GATnn'nnATC
Cuts at: 6235 6235
Size: 6241
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2134 2202 4048 4430
Size: 53 83 186 936 601 68 1846 382
Cuts at: 4430 5065 275
Size: 635 1451
Fragments arranged by size:
1846 1451 936 635 601 382 186 83
68 53
BsaWI w'CCGG_w
Cuts at: 2010 4674 5072 5346 5493 5904 2010
Size: 2664 398 274 147 411 2347
Fragments arranged by size:
2664 2347 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3179 3209 3332 3362 1178
Size: 30 165 30 1776 30 123 30 4057
Fragments arranged by size:
4057 1776 165 123 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2036 2861 3233 3329 1186
Size: 850 825 372 96 4098
Fragments arranged by size:
4098 850 825 372 96
BseYI C'CCAG_C
Cuts at: 2224 2234 2261 2719 5444 5940 2224
Size: 10 27 458 2725 496 2525
Fragments arranged by size:
2725 2525 496 458 27 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5668
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2065 3691 4452 5480 1540
Size: 275 186 64 1626 761 1028 2301
Fragments arranged by size:
2301 1626 1028 761 275 186 64
BsiHKAI G_wGCw'C
Cuts at: 3371 4302 4387 5458 3371
Size: 931 85 1071 4154
Fragments arranged by size:
4154 1071 931 85
BsmI GAATG_Cn'
Cuts at: 3752 3845 3752
Size: 93 6148
BsmBI CGTCTCn'nnnn_
Cuts at: 2466 2515 2466
Size: 49 6192
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2215 2231 2491 3335 3371 3560 4302
Size: 399 425 16 260 844 36 189 742
Cuts at: 4302 4387 5458 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 844 742 425 399 260 189
85 36 16
BspEI T'CCGG_A
Cuts at: 2010 5904 2010
Size: 3894 2347
BspHI T'CATG_A
Cuts at: 4130 5860 4130
Size: 1730 4511
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2385 2436 2759 3075 3285 3596 844
Size: 1541 51 323 316 210 311 3489
Fragments arranged by size:
3489 1541 323 316 311 210 51
BsrBI CCG'CTC
Cuts at: 1394 1823 4128 1394
Size: 429 2305 3507
Fragments arranged by size:
3507 2305 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4736 4910 65
Size: 1183 3488 174 1396
Fragments arranged by size:
3488 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2118 3168 4885 5072 1700
Size: 110 308 1050 1717 187 2869
Fragments arranged by size:
2869 1717 1050 308 187 110
BsrGI T'GTAC_A
Cuts at: 96 2799 96
Size: 2703 3538
BssHII G'CGCG_C
Cuts at: 1925 2109 2328 1925
Size: 184 219 5838
Fragments arranged by size:
5838 219 184
BssSI C'ACGA_G
Cuts at: 2357 2480 3389 4301 5313 2357
Size: 123 909 912 1012 3285
Fragments arranged by size:
3285 1012 912 909 123
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3375 794
Size: 2581 3660
BstBI TT'CG_AA
Cuts at: 5045 5045
Size: 6241
BstEII G'GTnAC_C
Cuts at: 1184 3427 1184
Size: 2243 3998
BstXI CCAn_nnnn'nTGG
Cuts at: 2311 2311
Size: 6241
BstYI r'GATC_y
Cuts at: 1972 3125 3571 3938 4323 4340 5792 5878 5890
Size: 1153 446 367 385 17 1452 86 12
Cuts at: 5890 6236 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1153 446 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3177 3177
Size: 6241
BtgI C'CryG_G
Cuts at: 513 1065 2059 2088 2443 2762 3045 3336 513
Size: 552 994 29 355 319 283 291 3418
Fragments arranged by size:
3418 994 552 355 319 291 283 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2021 487
Size: 683 56 795 4707
Fragments arranged by size:
4707 795 683 56
BtsI GCAGTG_nn'
Cuts at: 3065 3757 4551 4571 3065
Size: 692 794 20 4735
Fragments arranged by size:
4735 794 692 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6241
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6206
DraI TTT'AAA
Cuts at: 3545 3545
Size: 6241
DraIII CAC_nnn'GTG
Cuts at: 3391 3531 3391
Size: 140 6101
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2020 5248 809
Size: 507 704 3228 1802
Fragments arranged by size:
3228 1802 704 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2062 2365 3166
Size: 54 1425 50 275 18 232 303 801
Cuts at: 3166 3688 4577 6206 8
Size: 522 889 1629 43
Fragments arranged by size:
1629 1425 889 801 522 303 275 232
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2062 3688 1537
Size: 275 250 1626 4090
Fragments arranged by size:
4090 1626 275 250
EarI CTCTTCn'nnn_
Cuts at: 1353 2246 3243 4171 1353
Size: 893 997 928 3423
Fragments arranged by size:
3423 997 928 893
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4828 5202 5348 387
Size: 1064 138 3239 374 146 1280
Fragments arranged by size:
3239 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2399 2573 2640 3032 3206 3259 3274
Size: 393 767 174 67 392 174 53 15
Cuts at: 3274 3443 3478 4304 4901 5688 5956 1239
Size: 169 35 826 597 787 268 1524
Fragments arranged by size:
1524 826 787 767 597 393 392 268
174 174 169 67 53 35 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2381 3215 2381
Size: 834 5407
EcoO109I rG'GnC_Cy
Cuts at: 2335 2386 2911 3451 3462 2335
Size: 51 525 540 11 5114
Fragments arranged by size:
5114 540 525 51 11
EcoRI G'AATT_C
Cuts at: 3550 3928 3550
Size: 378 5863
EcoRV GAT'ATC
Cuts at: 2998 2998
Size: 6241
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3281 3281
Size: 6241
FspI TGC'GCA
Cuts at: 2705 4747 2705
Size: 2042 4199
HaeII r_GCGC'y
Cuts at: 1678 2205 2268 5388 1678
Size: 527 63 3120 2531
Fragments arranged by size:
3120 2531 527 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3179 3211 4882 4914 4956
Size: 32 135 32 1607 32 1671 32 42
Cuts at: 4956 4988 1373
Size: 32 2626
Fragments arranged by size:
2626 1671 1607 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2425 3585 3831 669
Size: 988 281 487 1160 246 3079
Fragments arranged by size:
3079 1160 988 487 281 246
HindIII A'AGCT_T
Cuts at: 748 2824 748
Size: 2076 4165
HpaI GTT'AAC
Cuts at: 2425 3831 2425
Size: 1406 4835
KasI G'GCGC_C
Cuts at: 2201 2201
Size: 6241
KpnI G_GTAC'C
Cuts at: 3566 3566
Size: 6241
MfeI C'AATT_G
Cuts at: 3840 3840
Size: 6241
MluI A'CGCG_T
Cuts at: 6117 6117
Size: 6241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5355 5539 1305
Size: 4050 184 2007
Fragments arranged by size:
4050 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4419
Fragments arranged by size:
4419 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2660 3029 3050 4199 4558 4717 518
Size: 648 1494 369 21 1149 359 159 2042
Fragments arranged by size:
2042 1494 1149 648 369 359 159 21
MspA1I CmG'CkG
Cuts at: 1606 2061 2618 2814 2954 3657 4334 5482 5727
Size: 455 557 196 140 703 677 1148 245
Cuts at: 5727 1606
Size: 2120
Fragments arranged by size:
2120 1148 703 677 557 455 245 196
140
NaeI GCC'GGC
Cuts at: 2120 2120
Size: 6241
NarI GG'CG_CC
Cuts at: 2202 2202
Size: 6241
NcoI C'CATG_G
Cuts at: 513 1065 2443 2762 3045 513
Size: 552 1378 319 283 3709
Fragments arranged by size:
3709 1378 552 319 283
NdeI CA'TA_TG
Cuts at: 387 3355 387
Size: 2968 3273
NgoMIV G'CCGG_C
Cuts at: 2118 2118
Size: 6241
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6241
NotI GC'GGCC_GC
Cuts at: 3688 3688
Size: 6241
NsiI A_TGCA'T
Cuts at: 2890 2890
Size: 6241
NspI r_CATG'y
Cuts at: 1132 1229 2083 2581 2800 3599 5144 1132
Size: 97 854 498 219 799 1545 2229
Fragments arranged by size:
2229 1545 854 799 498 219 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6241
PciI A'CATG_T
Cuts at: 2796 5140 2796
Size: 2344 3897
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6241
PfoI T'CCnGG_A
Cuts at: 2913 2913
Size: 6241
PmeI GTTT'AAAC
Cuts at: 3545 3545
Size: 6241
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4310 4342 5823 5855 4310
Size: 32 1481 32 4696
Fragments arranged by size:
4696 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2386 2911 3451 3462 2386
Size: 525 540 11 5165
Fragments arranged by size:
5165 540 525 11
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6241
PsiI TTA'TAA
Cuts at: 3811 3811
Size: 6241
PspXI vC'TCGA_Gb
Cuts at: 2600 2600
Size: 6241
PstI C_TGCA'G
Cuts at: 830 2756 3006 3060 3593 830
Size: 1926 250 54 533 3478
Fragments arranged by size:
3478 1926 533 250 54
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6241
PvuII CAG'CTG
Cuts at: 2618 2954 2618
Size: 336 5905
SacII CC_GC'GG
Cuts at: 2062 2062
Size: 6241
SalI G'TCGA_C
Cuts at: 1936 3583 1936
Size: 1647 4594
SanDI GG'GwC_CC
Cuts at: 3451 3451
Size: 6241
SapI GCTCTTCn'nnn_
Cuts at: 3243 3243
Size: 6241
SbfI CC_TGCA'GG
Cuts at: 3593 3593
Size: 6241
ScaI AGT'ACT
Cuts at: 4489 6201 4489
Size: 1712 4529
SfcI C'TryA_G
Cuts at: 826 1045 2752 3002 3056 3589 4724 5405 5596
Size: 219 1707 250 54 533 1135 681 191
Cuts at: 5596 826
Size: 1471
Fragments arranged by size:
1707 1471 1135 681 533 250 219 191
54
SfoI GGC'GCC
Cuts at: 2203 2203
Size: 6241
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6241
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6241
SmaI CCC'GGG
Cuts at: 1872 3568 1872
Size: 1696 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2600 4345 5246 5508 820
Size: 197 926 657 1745 901 262 1553
Fragments arranged by size:
1745 1553 926 901 657 262 197
SnaBI TAC'GTA
Cuts at: 493 4032 493
Size: 3539 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6241
SphI G_CATG'C
Cuts at: 1132 3599 1132
Size: 2467 3774
SspI AAT'ATT
Cuts at: 5 52 4165 5
Size: 47 4113 2081
Fragments arranged by size:
4113 2081 47
StuI AGG'CCT
Cuts at: 1517 2283 3495 1517
Size: 766 1212 4263
Fragments arranged by size:
4263 1212 766
StyI C'CwwG_G
Cuts at: 513 1065 2443 2762 3045 3680 513
Size: 552 1378 319 283 635 3074
Fragments arranged by size:
3074 1378 635 552 319 283
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2474 3542 4268 4285 4438 4623 5068
Size: 1255 894 1068 726 17 153 185 445
Cuts at: 5068 325
Size: 1498
Fragments arranged by size:
1498 1255 1068 894 726 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2756 2799 4487
Size: 274 80 33 51 1443 779 43 1688
Cuts at: 4487 6199 96
Size: 1712 138
Fragments arranged by size:
1712 1688 1443 779 274 138 80 51
43 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6201 6233 6201
Size: 32 6209
Tth111I GACn'n_nGTC
Cuts at: 1316 3040 1316
Size: 1724 4517
XbaI T'CTAG_A
Cuts at: 3577 3577
Size: 6241
XhoI C'TCGA_G
Cuts at: 1943 2600 1943
Size: 657 5584
XmaI C'CCGG_G
Cuts at: 1870 3566 1870
Size: 1696 4545
XmnI GAAnn'nnTTC
Cuts at: 3252 4370 3252
Size: 1118 5123
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4049 276
Size: 53 83 186 936 2515 2468
Fragments arranged by size:
2515 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI PvuII SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BsiWI BstZ17I
EcoICRI FseI FspAI NruI PacI PmlI PspOMI PsrI
RsrII SacI SexAI SfiI SrfI SwaI XcmI