Restriction map for
pFN21AE1956
(Circular) (Six-base) MAPSORT of: pFN21AE1956.seq Check: 7670 from: 1 to: 6244
pFN21AE1956 6244 bp
With 182 enzymes: SgfI *
March 28, 2012 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4054 278
Size: 53 83 186 936 2518 2468
Fragments arranged by size:
2518 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2556 3587 1937
Size: 619 1031 4594
Fragments arranged by size:
4594 1031 619
Acc65I G'GTAC_C
Cuts at: 3565 3565
Size: 6244
AclI AA'CG_TT
Cuts at: 1563 4371 4744 1563
Size: 2808 373 3063
Fragments arranged by size:
3063 2808 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3144 4307 5691 5959 1632
Size: 1512 1163 1384 268 1917
Fragments arranged by size:
1917 1512 1384 1163 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6047
AflIII A'CryG_T
Cuts at: 1203 2384 5143 6120 1203
Size: 1181 2759 977 1327
Fragments arranged by size:
2759 1327 1181 977
AgeI A'CCGG_T
Cuts at: 5075 5075
Size: 6244
AhdI GACnn_n'nnGTC
Cuts at: 2650 4973 2650
Size: 2323 3921
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6244
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6244
AlwNI CAG_nnn'CTG
Cuts at: 2440 2524 3004 3987 5559 2440
Size: 84 480 983 1572 3125
Fragments arranged by size:
3125 1572 983 480 84
ApaI G_GGCC'C
Cuts at: 3326 3326
Size: 6244
ApaLI G'TGCA_C
Cuts at: 4301 5457 4301
Size: 1156 5088
ApoI r'AATT_y
Cuts at: 1493 2973 3553 3779 3931 6040 1493
Size: 1480 580 226 152 2109 1697
Fragments arranged by size:
2109 1697 1480 580 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4798 160
Size: 872 3766 1606
Fragments arranged by size:
3766 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6244
AvaI C'yCGr_G
Cuts at: 1870 1943 2071 2274 3569 1870
Size: 73 128 203 1295 4545
Fragments arranged by size:
4545 1295 203 128 73
BamHI G'GATC_C
Cuts at: 2098 2830 3270 3574 3941 2098
Size: 732 440 304 367 4401
Fragments arranged by size:
4401 732 440 367 304
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2058 3565 5020 618
Size: 325 217 627 271 1507 1455 1842
Fragments arranged by size:
1842 1507 1455 627 325 271 217
BanII G_rGCy'C
Cuts at: 1391 3326 3526 3563 1391
Size: 1935 200 37 4072
Fragments arranged by size:
4072 1935 200 37
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2513 928
Size: 971 614 4659
Fragments arranged by size:
4659 971 614
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4435 4469 1903
Size: 34 2498 34 3678
Fragments arranged by size:
3678 2498 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4136 5346 4136
Size: 1210 5034
BclI T'GATC_A
Cuts at: 1548 2622 1548
Size: 1074 5170
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2039 4855
Size: 107 122 71 631 605 337 30 2816
Cuts at: 4855 136
Size: 1525
Fragments arranged by size:
2816 1525 631 605 337 122 107 71
30
BglII A'GATC_T
Cuts at: 6239 6239
Size: 6244
BlpI GC'TnA_GC
Cuts at: 3661 3661
Size: 6244
Bme1580I G_kGCm'C
Cuts at: 1790 2126 3326 4305 5461 1790
Size: 336 1200 979 1156 2573
Fragments arranged by size:
2573 1200 1156 979 336
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6244
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2422 2455 2469
Size: 416 53 83 399 24 1005 33 14
Cuts at: 2469 2512 3975 4933 442
Size: 43 1463 958 1753
Fragments arranged by size:
1753 1463 1005 958 416 399 83 53
43 33 24 14
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6244
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1999 4904 1239
Size: 760 2905 2579
Fragments arranged by size:
2905 2579 760
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4369 5234 5532 4369
Size: 865 298 5081
Fragments arranged by size:
5081 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3452 4907 882
Size: 617 1953 1455 2219
Fragments arranged by size:
2219 1953 1455 617
BsaAI yAC'GTr
Cuts at: 493 1204 4035 493
Size: 711 2831 2702
Fragments arranged by size:
2831 2702 711
BsaBI GATnn'nnATC
Cuts at: 6238 6238
Size: 6244
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4051 4433 5068 275
Size: 53 83 186 936 2518 382 635 1451
Fragments arranged by size:
2518 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4677 5075 5349 5496 5907 4677
Size: 398 274 147 411 5014
Fragments arranged by size:
5014 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2818 2848 1178
Size: 30 165 30 1415 30 4574
Fragments arranged by size:
4574 1415 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6244
BseYI C'CCAG_C
Cuts at: 5447 5943 5447
Size: 496 5748
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5671
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3694 4455 5483 1540
Size: 275 186 1693 761 1028 2301
Fragments arranged by size:
2301 1693 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3192 4305 4390 5461 3192
Size: 1113 85 1071 3975
Fragments arranged by size:
3975 1113 1071 85
BsmI GAATG_Cn'
Cuts at: 3755 3848 3755
Size: 93 6151
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2126 3192 3326 3526 3563 4305 4390
Size: 399 336 1066 134 200 37 742 85
Cuts at: 4390 5461 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1066 742 399 336 200 134
85 37
BspEI T'CCGG_A
Cuts at: 5907 5907
Size: 6244
BspHI T'CATG_A
Cuts at: 4133 5863 4133
Size: 1730 4514
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3599 844
Size: 2755 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4131 1394
Size: 429 2308 3507
Fragments arranged by size:
3507 2308 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3232 3384 4739 4913 65
Size: 1183 1984 152 1355 174 1396
Fragments arranged by size:
1984 1396 1355 1183 174 152
BsrFI r'CCGG_y
Cuts at: 1700 1810 4888 5075 1700
Size: 110 3078 187 2869
Fragments arranged by size:
3078 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6244
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6244
BssSI C'ACGA_G
Cuts at: 4304 5316 4304
Size: 1012 5232
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6244
BstBI TT'CG_AA
Cuts at: 5048 5048
Size: 6244
BstEII G'GTnAC_C
Cuts at: 1184 2155 1184
Size: 971 5273
BstXI CCAn_nnnn'nTGG
Cuts at: 2412 2412
Size: 6244
BstYI r'GATC_y
Cuts at: 1972 2098 2830 3270 3574 3941 4326 4343 5795
Size: 126 732 440 304 367 385 17 1452
Cuts at: 5795 5881 5893 6239 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 732 440 385 367 346 304
126 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2034 513
Size: 552 939 30 4723
Fragments arranged by size:
4723 939 552 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5505
Fragments arranged by size:
5505 683 56
BtsI GCAGTG_nn'
Cuts at: 2327 3760 4554 4574 2327
Size: 1433 794 20 3997
Fragments arranged by size:
3997 1433 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6244
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6209
DraI TTT'AAA
Cuts at: 3548 3548
Size: 6244
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5251 809
Size: 507 3935 1802
Fragments arranged by size:
3935 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3691 4580 6209
Size: 54 1425 50 275 18 1861 889 1629
Cuts at: 6209 8
Size: 43
Fragments arranged by size:
1861 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3691 1537
Size: 275 1879 4090
Fragments arranged by size:
4090 1879 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2145 2578 4174 1353
Size: 792 433 1596 3423
Fragments arranged by size:
3423 1596 792 433
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3087 4831 5205 5351 387
Size: 1064 138 1498 1744 374 146 1280
Fragments arranged by size:
1744 1498 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1999 3144 4307 4904 5691 5959 1239
Size: 393 367 1145 1163 597 787 268 1524
Fragments arranged by size:
1524 1163 1145 787 597 393 367 268
EcoO109I rG'GnC_Cy
Cuts at: 3322 3323 3322
Size: 1 6243
EcoRI G'AATT_C
Cuts at: 3553 3931 3553
Size: 378 5866
FspI TGC'GCA
Cuts at: 4750 4750
Size: 6244
HaeII r_GCGC'y
Cuts at: 1678 5391 1678
Size: 3713 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2636 2668 2818 2850 4885
Size: 32 135 32 1064 32 150 32 2035
Cuts at: 4885 4917 4959 4991 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2035 1064 150 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2480 2631 3155 3588 3834 669
Size: 988 281 542 151 524 433 246 3079
Fragments arranged by size:
3079 988 542 524 433 281 246 151
HindIII A'AGCT_T
Cuts at: 748 2992 748
Size: 2244 4000
HpaI GTT'AAC
Cuts at: 3834 3834
Size: 6244
KpnI G_GTAC'C
Cuts at: 3569 3569
Size: 6244
MfeI C'AATT_G
Cuts at: 3843 3843
Size: 6244
MluI A'CGCG_T
Cuts at: 6120 6120
Size: 6244
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2578 5358 5542 1305
Size: 1273 2780 184 2007
Fragments arranged by size:
2780 2007 1273 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4422
Fragments arranged by size:
4422 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2822 3315 4202 4561 4720 518
Size: 648 1656 493 887 359 159 2042
Fragments arranged by size:
2042 1656 887 648 493 359 159
MspA1I CmG'CkG
Cuts at: 1606 2119 3660 4337 5485 5730 1606
Size: 513 1541 677 1148 245 2120
Fragments arranged by size:
2120 1541 1148 677 513 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4753
Fragments arranged by size:
4753 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6244
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6244
NotI GC'GGCC_GC
Cuts at: 3691 3691
Size: 6244
NspI r_CATG'y
Cuts at: 1132 1229 2388 3602 5147 1132
Size: 97 1159 1214 1545 2229
Fragments arranged by size:
2229 1545 1214 1159 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6244
PciI A'CATG_T
Cuts at: 2384 5143 2384
Size: 2759 3485
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2943 3004 1108
Size: 1835 61 4348
Fragments arranged by size:
4348 1835 61
PfoI T'CCnGG_A
Cuts at: 2015 3266 2015
Size: 1251 4993
PmeI GTTT'AAAC
Cuts at: 3548 3548
Size: 6244
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4313 4345 5826 5858 4313
Size: 32 1481 32 4699
Fragments arranged by size:
4699 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6244
PsiI TTA'TAA
Cuts at: 2730 3042 3814 2730
Size: 312 772 5160
Fragments arranged by size:
5160 772 312
PspOMI G'GGCC_C
Cuts at: 3322 3322
Size: 6244
PstI C_TGCA'G
Cuts at: 830 3596 830
Size: 2766 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6244
SalI G'TCGA_C
Cuts at: 1936 3586 1936
Size: 1650 4594
SbfI CC_TGCA'GG
Cuts at: 3596 3596
Size: 6244
ScaI AGT'ACT
Cuts at: 2589 3373 4492 6204 2589
Size: 784 1119 1712 2629
Fragments arranged by size:
2629 1712 1119 784
SexAI A'CCwGG_T
Cuts at: 3012 3012
Size: 6244
SfcI C'TryA_G
Cuts at: 826 1045 2203 3277 3592 4727 5408 5599 826
Size: 219 1158 1074 315 1135 681 191 1471
Fragments arranged by size:
1471 1158 1135 1074 681 315 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6244
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6244
SmaI CCC'GGG
Cuts at: 1872 3571 1872
Size: 1699 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4348 5249 5511 820
Size: 197 926 2405 901 262 1553
Fragments arranged by size:
2405 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4035 493
Size: 3542 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6244
SphI G_CATG'C
Cuts at: 1132 3602 1132
Size: 2470 3774
SspI AAT'ATT
Cuts at: 5 52 4168 5
Size: 47 4116 2081
Fragments arranged by size:
4116 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6244
StyI C'CwwG_G
Cuts at: 513 1065 2004 2161 2606 3210 3683 513
Size: 552 939 157 445 604 473 3074
Fragments arranged by size:
3074 939 604 552 473 445 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4271 4288 4441 4626 5071 325
Size: 1255 2691 17 153 185 445 1498
Fragments arranged by size:
2691 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2587 3371 4490
Size: 274 80 33 51 1443 610 784 1119
Cuts at: 4490 6202 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1119 784 610 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2067 2099 3079 3111 6204 6236 2067
Size: 32 980 32 3093 32 2075
Fragments arranged by size:
3093 2075 980 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6244
XbaI T'CTAG_A
Cuts at: 3169 3580 3169
Size: 411 5833
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3533 3533
Size: 6244
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6244
XmaI C'CCGG_G
Cuts at: 1870 3569 1870
Size: 1699 4545
XmnI GAAnn'nnTTC
Cuts at: 4373 4373
Size: 6244
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4052 276
Size: 53 83 186 936 2518 2468
Fragments arranged by size:
2518 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SfiI
SfoI SrfI SwaI