Restriction map for
pFN21AE1953
(Circular) (Six-base) MAPSORT of: pFN21AE1953.seq Check: 124 from: 1 to: 6232
pFN21AE1953 6232 bp
With 182 enzymes: SgfI *
August 16, 2010 14:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4042 278
Size: 53 83 186 936 2506 2468
Fragments arranged by size:
2506 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2410 2861 3575 1937
Size: 473 451 714 4594
Fragments arranged by size:
4594 714 473 451
Acc65I G'GTAC_C
Cuts at: 3553 3553
Size: 6232
AclI AA'CG_TT
Cuts at: 1563 4359 4732 1563
Size: 2796 373 3063
Fragments arranged by size:
3063 2796 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2580 2598 3510 4295 5679 5947 1632
Size: 948 18 912 785 1384 268 1917
Fragments arranged by size:
1917 1384 948 912 785 268 18
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6035
AflIII A'CryG_T
Cuts at: 1203 2377 5131 6108 1203
Size: 1174 2754 977 1327
Fragments arranged by size:
2754 1327 1174 977
AgeI A'CCGG_T
Cuts at: 5063 5063
Size: 6232
AhdI GACnn_n'nnGTC
Cuts at: 4961 4961
Size: 6232
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6232
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6232
AlwNI CAG_nnn'CTG
Cuts at: 3118 3975 5547 3118
Size: 857 1572 3803
Fragments arranged by size:
3803 1572 857
ApaI G_GGCC'C
Cuts at: 2937 2937
Size: 6232
ApaLI G'TGCA_C
Cuts at: 3149 4289 5445 3149
Size: 1140 1156 3936
Fragments arranged by size:
3936 1156 1140
ApoI r'AATT_y
Cuts at: 1493 2749 2822 3541 3767 3919 6028 1493
Size: 1256 73 719 226 152 2109 1697
Fragments arranged by size:
2109 1697 1256 719 226 152 73
AseI AT'TA_AT
Cuts at: 160 1032 4786 160
Size: 872 3754 1606
Fragments arranged by size:
3754 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6232
AvaI C'yCGr_G
Cuts at: 1870 1943 3557 1870
Size: 73 1614 4545
Fragments arranged by size:
4545 1614 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2860 2893 2860
Size: 33 6199
BamHI G'GATC_C
Cuts at: 2425 3562 3929 2425
Size: 1137 367 4728
Fragments arranged by size:
4728 1137 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3553 5008 618
Size: 325 217 627 1766 1455 1842
Fragments arranged by size:
1842 1766 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2937 3551 1391
Size: 1546 614 4072
Fragments arranged by size:
4072 1546 614
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3049 928
Size: 971 1150 4111
Fragments arranged by size:
4111 1150 971
BbvCI CC'TCA_GC
Cuts at: 2707 2707
Size: 6232
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4423 4457 1903
Size: 34 2486 34 3678
Fragments arranged by size:
3678 2486 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4124 5334 4124
Size: 1210 5022
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6232
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4843 136
Size: 107 122 71 631 605 3171 1525
Fragments arranged by size:
3171 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2505 6227 2505
Size: 3722 2510
BlpI GC'TnA_GC
Cuts at: 3649 3649
Size: 6232
Bme1580I G_kGCm'C
Cuts at: 1790 2937 3153 4293 5449 1790
Size: 1147 216 1140 1156 2573
Fragments arranged by size:
2573 1156 1147 1140 216
BmgBI CAC'GTC
Cuts at: 1318 2378 1318
Size: 1060 5172
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3963 4921 442
Size: 416 53 83 399 24 2546 958 1753
Fragments arranged by size:
2546 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2793 3330 3353 4892 1239
Size: 1554 537 23 1539 2579
Fragments arranged by size:
2579 1554 1539 537 23
Bpu10I CC'TnA_GC
Cuts at: 2707 2707
Size: 6232
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2581 4357 5222 5520 2581
Size: 1776 865 298 3293
Fragments arranged by size:
3293 1776 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4895 882
Size: 617 3396 2219
Fragments arranged by size:
3396 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4023 493
Size: 711 2819 2702
Fragments arranged by size:
2819 2702 711
BsaBI GATnn'nnATC
Cuts at: 2988 6226 2988
Size: 3238 2994
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4039 4421 5056 275
Size: 53 83 186 936 2506 382 635 1451
Fragments arranged by size:
2506 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2392 4665 5063 5337 5484 5895 2392
Size: 2273 398 274 147 411 2729
Fragments arranged by size:
2729 2273 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6007
Fragments arranged by size:
6007 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6232
BseYI C'CCAG_C
Cuts at: 2924 3118 5435 5931 2924
Size: 194 2317 496 3225
Fragments arranged by size:
3225 2317 496 194
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2950 1163
Size: 573 1214 4445
Fragments arranged by size:
4445 1214 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3682 4443 5471 1540
Size: 275 186 1681 761 1028 2301
Fragments arranged by size:
2301 1681 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3153 4293 4378 5449 3153
Size: 1140 85 1071 3936
Fragments arranged by size:
3936 1140 1071 85
BsmI GAATG_Cn'
Cuts at: 3743 3836 3743
Size: 93 6139
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2937 3153 3551 4293 4378 5449 1391
Size: 399 1147 216 398 742 85 1071 2174
Fragments arranged by size:
2174 1147 1071 742 399 398 216 85
BspEI T'CCGG_A
Cuts at: 2392 5895 2392
Size: 3503 2729
BspHI T'CATG_A
Cuts at: 2446 3159 3454 4121 5851 2446
Size: 713 295 667 1730 2827
Fragments arranged by size:
2827 1730 713 667 295
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3587 844
Size: 2743 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4119 1394
Size: 429 2296 3507
Fragments arranged by size:
3507 2296 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2354 4727 4901 65
Size: 1183 1106 2373 174 1396
Fragments arranged by size:
2373 1396 1183 1106 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4876 5063 1700
Size: 110 3066 187 2869
Fragments arranged by size:
3066 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3015 96
Size: 2919 3313
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6232
BssSI C'ACGA_G
Cuts at: 4292 5304 4292
Size: 1012 5220
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6232
BstBI TT'CG_AA
Cuts at: 5036 5036
Size: 6232
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6232
BstXI CCAn_nnnn'nTGG
Cuts at: 2106 2106
Size: 6232
BstYI r'GATC_y
Cuts at: 1972 2016 2389 2425 2505 3460 3562 3929 4314
Size: 44 373 36 80 955 102 367 385
Cuts at: 4314 4331 5783 5869 5881 6227 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 955 385 373 367 346 102
86 80 44 36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4741
Fragments arranged by size:
4741 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5493
Fragments arranged by size:
5493 683 56
BtsI GCAGTG_nn'
Cuts at: 3238 3748 4542 4562 3238
Size: 510 794 20 4908
Fragments arranged by size:
4908 794 510 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6232
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6197
DraI TTT'AAA
Cuts at: 2275 3536 2275
Size: 1261 4971
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5239 809
Size: 507 3923 1802
Fragments arranged by size:
3923 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2671 3679 4568
Size: 54 1425 50 275 18 841 1008 889
Cuts at: 4568 6197 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1008 889 841 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3679 1537
Size: 275 1867 4090
Fragments arranged by size:
4090 1867 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3096 4162 1353
Size: 1743 1066 3423
Fragments arranged by size:
3423 1743 1066
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4819 5193 5339 387
Size: 1064 138 3230 374 146 1280
Fragments arranged by size:
3230 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2580 2598 2793 3330 3353 3510 4295
Size: 393 948 18 195 537 23 157 785
Cuts at: 4295 4892 5679 5947 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 948 787 785 597 537 393 268
195 157 23 18
EcoO109I rG'GnC_Cy
Cuts at: 2934 2934
Size: 6232
EcoRI G'AATT_C
Cuts at: 3541 3919 3541
Size: 378 5854
FspI TGC'GCA
Cuts at: 4738 4738
Size: 6232
HaeII r_GCGC'y
Cuts at: 1678 2365 5379 1678
Size: 687 3014 2531
Fragments arranged by size:
3014 2531 687
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2374 2406 3427 3459 4873
Size: 32 135 32 802 32 1021 32 1414
Cuts at: 4873 4905 4947 4979 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1414 1021 802 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2287 3576 3822 669
Size: 988 281 349 1289 246 3079
Fragments arranged by size:
3079 1289 988 349 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6232
HpaI GTT'AAC
Cuts at: 3822 3822
Size: 6232
KpnI G_GTAC'C
Cuts at: 3557 3557
Size: 6232
MfeI C'AATT_G
Cuts at: 3831 3831
Size: 6232
MluI A'CGCG_T
Cuts at: 6108 6108
Size: 6232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2403 5346 5530 1305
Size: 1098 2943 184 2007
Fragments arranged by size:
2943 2007 1098 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2673 10
Size: 54 1425 343 841 3569
Fragments arranged by size:
3569 1425 841 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2294 4190 4549 4708 518
Size: 648 1128 1896 359 159 2042
Fragments arranged by size:
2042 1896 1128 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2650 3115 3648 4325 5473 5718 1606
Size: 1044 465 533 677 1148 245 2120
Fragments arranged by size:
2120 1148 1044 677 533 465 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4741
Fragments arranged by size:
4741 939 552
NdeI CA'TA_TG
Cuts at: 387 2533 387
Size: 2146 4086
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6232
NotI GC'GGCC_GC
Cuts at: 3679 3679
Size: 6232
NspI r_CATG'y
Cuts at: 1132 1229 3342 3590 5135 1132
Size: 97 2113 248 1545 2229
Fragments arranged by size:
2229 2113 1545 248 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6232
PciI A'CATG_T
Cuts at: 5131 5131
Size: 6232
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6232
PfoI T'CCnGG_A
Cuts at: 2695 2695
Size: 6232
PmeI GTTT'AAAC
Cuts at: 3536 3536
Size: 6232
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4301 4333 5814 5846 4301
Size: 32 1481 32 4687
Fragments arranged by size:
4687 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6232
PsiI TTA'TAA
Cuts at: 3802 3802
Size: 6232
PspOMI G'GGCC_C
Cuts at: 2933 2933
Size: 6232
PstI C_TGCA'G
Cuts at: 830 3584 830
Size: 2754 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6232
PvuII CAG'CTG
Cuts at: 2650 3115 2650
Size: 465 5767
SalI G'TCGA_C
Cuts at: 1936 3574 1936
Size: 1638 4594
SbfI CC_TGCA'GG
Cuts at: 3584 3584
Size: 6232
ScaI AGT'ACT
Cuts at: 4480 6192 4480
Size: 1712 4520
SexAI A'CCwGG_T
Cuts at: 2140 3354 2140
Size: 1214 5018
SfcI C'TryA_G
Cuts at: 826 1045 2862 3180 3580 4715 5396 5587 826
Size: 219 1817 318 400 1135 681 191 1471
Fragments arranged by size:
1817 1471 1135 681 400 318 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6232
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6232
SmaI CCC'GGG
Cuts at: 1872 3559 1872
Size: 1687 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2560 4336 5237 5499 820
Size: 197 926 617 1776 901 262 1553
Fragments arranged by size:
1776 1553 926 901 617 262 197
SnaBI TAC'GTA
Cuts at: 493 4023 493
Size: 3530 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6232
SphI G_CATG'C
Cuts at: 1132 3342 3590 1132
Size: 2210 248 3774
Fragments arranged by size:
3774 2210 248
SspI AAT'ATT
Cuts at: 5 52 2439 2731 4156 5
Size: 47 2387 292 1425 2081
Fragments arranged by size:
2387 2081 1425 292 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6232
StyI C'CwwG_G
Cuts at: 513 1065 2004 3085 3671 513
Size: 552 939 1081 586 3074
Fragments arranged by size:
3074 1081 939 586 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4259 4276 4429 4614 5059 325
Size: 1255 2679 17 153 185 445 1498
Fragments arranged by size:
2679 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3015 4478 6190
Size: 274 80 33 51 1443 1038 1463 1712
Cuts at: 6190 96
Size: 138
Fragments arranged by size:
1712 1463 1443 1038 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3374 3406 6192 6224 3374
Size: 32 2786 32 3382
Fragments arranged by size:
3382 2786 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6232
XbaI T'CTAG_A
Cuts at: 3568 3568
Size: 6232
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6232
XmaI C'CCGG_G
Cuts at: 1870 3557 1870
Size: 1687 4545
XmnI GAAnn'nnTTC
Cuts at: 3056 3077 4361 3056
Size: 21 1284 4927
Fragments arranged by size:
4927 1284 21
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4040 276
Size: 53 83 186 936 2506 2468
Fragments arranged by size:
2506 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI