Restriction map for
pFN21AE1951
(Circular) (Six-base) MAPSORT of: pFN21AE1951.seq Check: 6258 from: 1 to: 6229
pFN21AE1951 6229 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3503 4039 278
Size: 53 83 186 936 1967 536 2468
Fragments arranged by size:
2468 1967 936 536 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3572 1937
Size: 1635 4594
Acc65I G'GTAC_C
Cuts at: 3550 3550
Size: 6229
AclI AA'CG_TT
Cuts at: 1563 4356 4729 1563
Size: 2793 373 3063
Fragments arranged by size:
3063 2793 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2573 2957 4292 5676 5944 1632
Size: 941 384 1335 1384 268 1917
Fragments arranged by size:
1917 1384 1335 941 384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6032
AflIII A'CryG_T
Cuts at: 1203 2430 2883 5128 6105 1203
Size: 1227 453 2245 977 1327
Fragments arranged by size:
2245 1327 1227 977 453
AgeI A'CCGG_T
Cuts at: 5060 5060
Size: 6229
AhdI GACnn_n'nnGTC
Cuts at: 4958 4958
Size: 6229
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6229
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2652 1184
Size: 1468 4761
AlwNI CAG_nnn'CTG
Cuts at: 2374 3197 3972 5544 2374
Size: 823 775 1572 3059
Fragments arranged by size:
3059 1572 823 775
ApaI G_GGCC'C
Cuts at: 2093 2093
Size: 6229
ApaLI G'TGCA_C
Cuts at: 4286 5442 4286
Size: 1156 5073
ApoI r'AATT_y
Cuts at: 1493 3538 3764 3916 6025 1493
Size: 2045 226 152 2109 1697
Fragments arranged by size:
2109 2045 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4783 160
Size: 872 3751 1606
Fragments arranged by size:
3751 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6229
AvaI C'yCGr_G
Cuts at: 1870 1943 2070 2126 2514 2576 3129 3554 1870
Size: 73 127 56 388 62 553 425 4545
Fragments arranged by size:
4545 553 425 388 127 73 62 56
BamHI G'GATC_C
Cuts at: 3559 3926 3559
Size: 367 5862
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2201 2325 2602 2655 3170
Size: 325 217 627 414 124 277 53 515
Cuts at: 3170 3550 5005 618
Size: 380 1455 1842
Fragments arranged by size:
1842 1455 627 515 414 380 325 277
217 124 53
BanII G_rGCy'C
Cuts at: 1391 2093 2195 2864 2954 3548 1391
Size: 702 102 669 90 594 4072
Fragments arranged by size:
4072 702 669 594 102 90
BbeI G_GCGC'C
Cuts at: 2205 2606 2205
Size: 401 5828
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5258
BbvCI CC'TCA_GC
Cuts at: 2240 2783 3014 2240
Size: 543 231 5455
Fragments arranged by size:
5455 543 231
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4420 4454 1903
Size: 34 2483 34 3678
Fragments arranged by size:
3678 2483 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4121 5331 4121
Size: 1210 5019
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6229
BfrBI ATG'CAT
Cuts at: 2629 3392 2629
Size: 763 5466
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2334 4840 136
Size: 107 122 71 631 605 662 2506 1525
Fragments arranged by size:
2506 1525 662 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2667 6224 2667
Size: 3557 2672
BlpI GC'TnA_GC
Cuts at: 2640 3646 2640
Size: 1006 5223
Bme1580I G_kGCm'C
Cuts at: 1790 2093 3414 4290 5446 1790
Size: 303 1321 876 1156 2573
Fragments arranged by size:
2573 1321 1156 876 303
BmgBI CAC'GTC
Cuts at: 1318 2461 1318
Size: 1143 5086
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2290 3960 4918
Size: 416 53 83 399 24 873 1670 958
Cuts at: 4918 442
Size: 1753
Fragments arranged by size:
1753 1670 958 873 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6229
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2237 2252 2729 2878 3359 4889 1239
Size: 998 15 477 149 481 1530 2579
Fragments arranged by size:
2579 1530 998 481 477 149 15
Bpu10I CC'TnA_GC
Cuts at: 2240 2783 3014 2240
Size: 543 231 5455
Fragments arranged by size:
5455 543 231
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4354 5219 5517 4354
Size: 865 298 5066
Fragments arranged by size:
5066 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2984 3119 3472 4892 882
Size: 617 1485 135 353 1420 2219
Fragments arranged by size:
2219 1485 1420 617 353 135
BsaAI yAC'GTr
Cuts at: 493 1204 4020 493
Size: 711 2816 2702
Fragments arranged by size:
2816 2702 711
BsaBI GATnn'nnATC
Cuts at: 6223 6223
Size: 6229
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2097 2202 2603 3500
Size: 53 83 186 936 564 105 401 897
Cuts at: 3500 4036 4418 5053 275
Size: 536 382 635 1451
Fragments arranged by size:
1451 936 897 635 564 536 401 382
186 105 83 53
BsaWI w'CCGG_w
Cuts at: 4662 5060 5334 5481 5892 4662
Size: 398 274 147 411 4999
Fragments arranged by size:
4999 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2243 2273 2373 2403 3467
Size: 30 165 30 840 30 100 30 1064
Cuts at: 3467 3497 1178
Size: 30 3910
Fragments arranged by size:
3910 1064 840 165 100 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2594 2798 1186
Size: 1408 204 4617
Fragments arranged by size:
4617 1408 204
BseYI C'CCAG_C
Cuts at: 2947 3349 5432 5928 2947
Size: 402 2083 496 3248
Fragments arranged by size:
3248 2083 496 402
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2462 1163
Size: 573 726 4930
Fragments arranged by size:
4930 726 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2049 3679 4440 5468 1540
Size: 275 186 48 1630 761 1028 2301
Fragments arranged by size:
2301 1630 1028 761 275 186 48
BsiHKAI G_wGCw'C
Cuts at: 2864 4290 4375 5446 2864
Size: 1426 85 1071 3647
Fragments arranged by size:
3647 1426 1071 85
BsmI GAATG_Cn'
Cuts at: 3740 3833 3740
Size: 93 6136
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2093 2195 2864 2954 3414 3548 4290
Size: 399 303 102 669 90 460 134 742
Cuts at: 4290 4375 5446 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 669 460 399 303 134
102 90 85
BspEI T'CCGG_A
Cuts at: 5892 5892
Size: 6229
BspHI T'CATG_A
Cuts at: 4118 5848 4118
Size: 1730 4499
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2484 2691 3584 844
Size: 1640 207 893 3489
Fragments arranged by size:
3489 1640 893 207
BsrBI CCG'CTC
Cuts at: 1394 1823 2806 3150 3483 4116 1394
Size: 429 983 344 333 633 3507
Fragments arranged by size:
3507 983 633 429 344 333
BsrDI GCAATG_nn'
Cuts at: 65 1248 2607 4724 4898 65
Size: 1183 1359 2117 174 1396
Fragments arranged by size:
2117 1396 1359 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2481 2571 2764 4873 5060 1700
Size: 110 671 90 193 2109 187 2869
Fragments arranged by size:
2869 2109 671 193 187 110 90
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6229
BssHII G'CGCG_C
Cuts at: 1925 2035 2037 2211 1925
Size: 110 2 174 5943
Fragments arranged by size:
5943 174 110 2
BssSI C'ACGA_G
Cuts at: 4289 5301 4289
Size: 1012 5217
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3389 794
Size: 2595 3634
BstBI TT'CG_AA
Cuts at: 5033 5033
Size: 6229
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6229
BstXI CCAn_nnnn'nTGG
Cuts at: 2263 2455 3373 2263
Size: 192 918 5119
Fragments arranged by size:
5119 918 192
BstYI r'GATC_y
Cuts at: 1972 2667 2733 3559 3926 4311 4328 5780 5866
Size: 695 66 826 367 385 17 1452 86
Cuts at: 5866 5878 6224 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 826 695 385 367 346 86
66 17 12
Bsu36I CC'TnA_GG
Cuts at: 3447 3447
Size: 6229
BtgI C'CryG_G
Cuts at: 513 1065 2004 2043 2524 3366 513
Size: 552 939 39 481 842 3376
Fragments arranged by size:
3376 939 842 552 481 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5490
Fragments arranged by size:
5490 683 56
BtsI GCAGTG_nn'
Cuts at: 3149 3745 4539 4559 3149
Size: 596 794 20 4819
Fragments arranged by size:
4819 794 596 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6229
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6194
DraI TTT'AAA
Cuts at: 3533 3533
Size: 6229
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5236 809
Size: 507 3920 1802
Fragments arranged by size:
3920 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 2295 2527
Size: 54 1425 50 275 18 216 249 232
Cuts at: 2527 3375 3676 4565 6194 8
Size: 848 301 889 1629 43
Fragments arranged by size:
1629 1425 889 848 301 275 249 232
216 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2046 3676 1537
Size: 275 234 1630 4090
Fragments arranged by size:
4090 1630 275 234
EarI CTCTTCn'nnn_
Cuts at: 1353 2006 4159 1353
Size: 653 2153 3423
Fragments arranged by size:
3423 2153 653
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3224 4816 5190 5336 387
Size: 1064 138 1635 1592 374 146 1280
Fragments arranged by size:
1635 1592 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2862 2862
Size: 6229
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2237 2252 2573 2729 2878 2957 3359
Size: 393 605 15 321 156 149 79 402
Cuts at: 3359 4292 4889 5676 5944 1239
Size: 933 597 787 268 1524
Fragments arranged by size:
1524 933 787 605 597 402 393 321
268 156 149 79 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2084 2084
Size: 6229
EcoO109I rG'GnC_Cy
Cuts at: 2089 2090 2443 2536 2089
Size: 1 353 93 5782
Fragments arranged by size:
5782 353 93 1
EcoRI G'AATT_C
Cuts at: 3538 3916 3538
Size: 378 5851
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2197 2929 3199 2197
Size: 732 270 5227
Fragments arranged by size:
5227 732 270
FspI TGC'GCA
Cuts at: 4735 4735
Size: 6229
HaeII r_GCGC'y
Cuts at: 1678 2164 2205 2606 3082 3118 5376 1678
Size: 486 41 401 476 36 2258 2531
Fragments arranged by size:
2531 2258 486 476 401 41 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4870 4902 4944 4976 1373
Size: 32 135 32 3298 32 42 32 2626
Fragments arranged by size:
3298 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3573 3819 669
Size: 988 281 1635 246 3079
Fragments arranged by size:
3079 1635 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6229
HpaI GTT'AAC
Cuts at: 3819 3819
Size: 6229
KasI G'GCGC_C
Cuts at: 2201 2602 2201
Size: 401 5828
KpnI G_GTAC'C
Cuts at: 3554 3554
Size: 6229
MfeI C'AATT_G
Cuts at: 3828 3828
Size: 6229
MluI A'CGCG_T
Cuts at: 6105 6105
Size: 6229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5343 5527 1305
Size: 4038 184 2007
Fragments arranged by size:
4038 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2297 10
Size: 54 1425 343 465 3942
Fragments arranged by size:
3942 1425 465 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2630 3371 4187 4546 4705 518
Size: 648 1464 741 816 359 159 2042
Fragments arranged by size:
2042 1464 816 741 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2045 2615 2722 3109 3310 3337 3380 3645
Size: 439 570 107 387 201 27 43 265
Cuts at: 3645 4322 5470 5715 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 570 439 387 265 245
201 107 43 27
NarI GG'CG_CC
Cuts at: 2202 2603 2202
Size: 401 5828
NcoI C'CATG_G
Cuts at: 513 1065 2004 3366 513
Size: 552 939 1362 3376
Fragments arranged by size:
3376 1362 939 552
NdeI CA'TA_TG
Cuts at: 387 2631 387
Size: 2244 3985
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6229
NotI GC'GGCC_GC
Cuts at: 3676 3676
Size: 6229
NsiI A_TGCA'T
Cuts at: 2631 3394 2631
Size: 763 5466
NspI r_CATG'y
Cuts at: 1132 1229 2272 2434 2887 3396 3410 3587 5132
Size: 97 1043 162 453 509 14 177 1545
Cuts at: 5132 1132
Size: 2229
Fragments arranged by size:
2229 1545 1043 509 453 177 162 97
14
PasI CC'CwG_GG
Cuts at: 1348 2086 2438 1348
Size: 738 352 5139
Fragments arranged by size:
5139 738 352
PciI A'CATG_T
Cuts at: 2430 2883 5128 2430
Size: 453 2245 3531
Fragments arranged by size:
3531 2245 453
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3055 1108
Size: 1947 4282
PfoI T'CCnGG_A
Cuts at: 3048 3048
Size: 6229
PmeI GTTT'AAAC
Cuts at: 3533 3533
Size: 6229
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4298 4330 5811 5843 4298
Size: 32 1481 32 4684
Fragments arranged by size:
4684 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2443 2536 2443
Size: 93 6136
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6229
PsiI TTA'TAA
Cuts at: 3799 3799
Size: 6229
PspOMI G'GGCC_C
Cuts at: 2089 2089
Size: 6229
PstI C_TGCA'G
Cuts at: 830 3144 3385 3581 830
Size: 2314 241 196 3478
Fragments arranged by size:
3478 2314 241 196
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6229
PvuII CAG'CTG
Cuts at: 2722 3109 3337 2722
Size: 387 228 5614
Fragments arranged by size:
5614 387 228
SacI G_AGCT'C
Cuts at: 2864 2864
Size: 6229
SacII CC_GC'GG
Cuts at: 2046 2046
Size: 6229
SalI G'TCGA_C
Cuts at: 1936 3571 1936
Size: 1635 4594
SanDI GG'GwC_CC
Cuts at: 2443 2443
Size: 6229
SapI GCTCTTCn'nnn_
Cuts at: 2006 2006
Size: 6229
SbfI CC_TGCA'GG
Cuts at: 3581 3581
Size: 6229
ScaI AGT'ACT
Cuts at: 4477 6189 4477
Size: 1712 4517
SexAI A'CCwGG_T
Cuts at: 3196 3196
Size: 6229
SfcI C'TryA_G
Cuts at: 826 1045 2695 3140 3381 3577 4712 5393 5584
Size: 219 1650 445 241 196 1135 681 191
Cuts at: 5584 826
Size: 1471
Fragments arranged by size:
1650 1471 1135 681 445 241 219 196
191
SfoI GGC'GCC
Cuts at: 2203 2604 2203
Size: 401 5828
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6229
SgrAI Cr'CCGG_yG
Cuts at: 1700 2764 1700
Size: 1064 5165
SmaI CCC'GGG
Cuts at: 1872 2072 2516 3131 3556 1872
Size: 200 444 615 425 4545
Fragments arranged by size:
4545 615 444 425 200
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4333 5234 5496 820
Size: 197 926 2390 901 262 1553
Fragments arranged by size:
2390 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4020 493
Size: 3527 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6229
SphI G_CATG'C
Cuts at: 1132 3587 1132
Size: 2455 3774
SspI AAT'ATT
Cuts at: 5 52 4153 5
Size: 47 4101 2081
Fragments arranged by size:
4101 2081 47
StuI AGG'CCT
Cuts at: 1517 2558 3038 1517
Size: 1041 480 4708
Fragments arranged by size:
4708 1041 480
StyI C'CwwG_G
Cuts at: 513 1065 2004 3174 3366 3432 3668 513
Size: 552 939 1170 192 66 236 3074
Fragments arranged by size:
3074 1170 939 552 236 192 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3301 4256 4273 4426 4611 5056 325
Size: 1255 1721 955 17 153 185 445 1498
Fragments arranged by size:
1721 1498 1255 955 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4475 6187 96
Size: 274 80 33 51 1443 2498 1712 138
Fragments arranged by size:
2498 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2243 2275 2373 2405 2435 2467 3216 3248 3467
Size: 32 98 32 30 32 749 32 219
Cuts at: 3467 3499 6189 6221 2243
Size: 32 2690 32 2251
Fragments arranged by size:
2690 2251 749 219 98 32 32 32
32 32 32 30
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6229
XbaI T'CTAG_A
Cuts at: 3565 3565
Size: 6229
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3181 3181
Size: 6229
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6229
XmaI C'CCGG_G
Cuts at: 1870 2070 2514 3129 3554 1870
Size: 200 444 615 425 4545
Fragments arranged by size:
4545 615 444 425 200
XmnI GAAnn'nnTTC
Cuts at: 4358 4358
Size: 6229
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3501 4037 276
Size: 53 83 186 936 1967 536 2468
Fragments arranged by size:
2468 1967 936 536 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BplI BsiWI
BsmBI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV
NruI PacI PmlI PspXI PsrI RsrII SfiI SrfI
SwaI