Restriction map for
pFN21AE1948
(Circular) (Six-base) MAPSORT of: pFN21AE1948.seq Check: 3258 from: 1 to: 6214
pFN21AE1948 6214 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4024 278
Size: 53 83 186 936 2488 2468
Fragments arranged by size:
2488 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3557 1937
Size: 1620 4594
Acc65I G'GTAC_C
Cuts at: 3430 3535 3430
Size: 105 6109
AclI AA'CG_TT
Cuts at: 1563 4341 4714 1563
Size: 2778 373 3063
Fragments arranged by size:
3063 2778 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3081 3357 4277 5661 5929 1632
Size: 1449 276 920 1384 268 1917
Fragments arranged by size:
1917 1449 1384 920 276 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6017
AflIII A'CryG_T
Cuts at: 1203 5113 6090 1203
Size: 3910 977 1327
Fragments arranged by size:
3910 1327 977
AgeI A'CCGG_T
Cuts at: 5045 5045
Size: 6214
AhdI GACnn_n'nnGTC
Cuts at: 3409 4943 3409
Size: 1534 4680
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6214
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6214
AlwNI CAG_nnn'CTG
Cuts at: 3957 5529 3957
Size: 1572 4642
ApaLI G'TGCA_C
Cuts at: 4271 5427 4271
Size: 1156 5058
ApoI r'AATT_y
Cuts at: 1493 2567 3395 3523 3749 3901 6010 1493
Size: 1074 828 128 226 152 2109 1697
Fragments arranged by size:
2109 1697 1074 828 226 152 128
AseI AT'TA_AT
Cuts at: 160 1032 4768 160
Size: 872 3736 1606
Fragments arranged by size:
3736 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6214
AvaI C'yCGr_G
Cuts at: 1870 1943 3539 1870
Size: 73 1596 4545
Fragments arranged by size:
4545 1596 73
BamHI G'GATC_C
Cuts at: 3544 3911 3544
Size: 367 5847
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2048 3246 3430 3535 4990
Size: 325 217 627 261 1198 184 105 1455
Cuts at: 4990 618
Size: 1842
Fragments arranged by size:
1842 1455 1198 627 325 261 217 184
105
BanII G_rGCy'C
Cuts at: 1391 3533 1391
Size: 2142 4072
BbeI G_GCGC'C
Cuts at: 2052 3250 2052
Size: 1198 5016
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5243
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2043 2077 4405 4439 1903
Size: 34 106 34 2328 34 3678
Fragments arranged by size:
3678 2328 106 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4106 5316 4106
Size: 1210 5004
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6214
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4825 136
Size: 107 122 71 631 605 3153 1525
Fragments arranged by size:
3153 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2754 6209 2754
Size: 3455 2759
BlpI GC'TnA_GC
Cuts at: 3631 3631
Size: 6214
Bme1580I G_kGCm'C
Cuts at: 1790 4275 5431 1790
Size: 2485 1156 2573
Fragments arranged by size:
2573 2485 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6214
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3945 4903 442
Size: 416 53 83 399 24 2528 958 1753
Fragments arranged by size:
2528 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6214
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2102 2540 4874 1239
Size: 863 438 2334 2579
Fragments arranged by size:
2579 2334 863 438
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2755 3065 4339 5204 5502 2755
Size: 310 1274 865 298 3467
Fragments arranged by size:
3467 1274 865 310 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4877 882
Size: 617 3378 2219
Fragments arranged by size:
3378 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4005 493
Size: 711 2801 2702
Fragments arranged by size:
2801 2702 711
BsaBI GATnn'nnATC
Cuts at: 6208 6208
Size: 6214
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2049 3247 4021 4403
Size: 53 83 186 936 516 1198 774 382
Cuts at: 4403 5038 275
Size: 635 1451
Fragments arranged by size:
1451 1198 936 774 635 516 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4647 5045 5319 5466 5877 4647
Size: 398 274 147 411 4984
Fragments arranged by size:
4984 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5989
Fragments arranged by size:
5989 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6214
BseYI C'CCAG_C
Cuts at: 5417 5913 5417
Size: 496 5718
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2047 2655 1163
Size: 573 311 608 4722
Fragments arranged by size:
4722 608 573 311
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3664 4425 5453 1540
Size: 275 186 1663 761 1028 2301
Fragments arranged by size:
2301 1663 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4275 4360 5431 4275
Size: 85 1071 5058
Fragments arranged by size:
5058 1071 85
BsmI GAATG_Cn'
Cuts at: 2329 3171 3353 3446 3725 3818 2329
Size: 842 182 93 279 93 4725
Fragments arranged by size:
4725 842 279 182 93 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3533 4275 4360 5431 1391
Size: 399 1743 742 85 1071 2174
Fragments arranged by size:
2174 1743 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5877 5877
Size: 6214
BspHI T'CATG_A
Cuts at: 4103 5833 4103
Size: 1730 4484
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2119 3569 844
Size: 1275 1450 3489
Fragments arranged by size:
3489 1450 1275
BsrBI CCG'CTC
Cuts at: 1394 1823 4101 1394
Size: 429 2278 3507
Fragments arranged by size:
3507 2278 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2430 2877 3089 4709 4883 65
Size: 1183 1182 447 212 1620 174 1396
Fragments arranged by size:
1620 1396 1183 1182 447 212 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4858 5045 1700
Size: 110 3048 187 2869
Fragments arranged by size:
3048 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6214
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6214
BssSI C'ACGA_G
Cuts at: 4274 5286 4274
Size: 1012 5202
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6214
BstBI TT'CG_AA
Cuts at: 3378 5018 3378
Size: 1640 4574
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6214
BstXI CCAn_nnnn'nTGG
Cuts at: 2316 2316
Size: 6214
BstYI r'GATC_y
Cuts at: 1972 2305 2754 2914 3544 3911 4296 4313 5765
Size: 333 449 160 630 367 385 17 1452
Cuts at: 5765 5851 5863 6209 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 630 449 385 367 346 333
160 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2040 2040
Size: 6214
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5662
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5475
Fragments arranged by size:
5475 683 56
BtsI GCAGTG_nn'
Cuts at: 2064 2650 2667 3730 4524 4544 2064
Size: 586 17 1063 794 20 3734
Fragments arranged by size:
3734 1063 794 586 20 17
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6214
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2113 2148 529
Size: 35 1549 35 4595
Fragments arranged by size:
4595 1549 35 35
DraI TTT'AAA
Cuts at: 3518 3518
Size: 6214
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5221 809
Size: 507 3905 1802
Fragments arranged by size:
3905 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2121 2963 3661
Size: 54 1425 50 275 18 291 842 698
Cuts at: 3661 4550 6179 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 842 698 291 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3661 1537
Size: 275 1849 4090
Fragments arranged by size:
4090 1849 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2466 3508 4144 1353
Size: 1113 1042 636 3423
Fragments arranged by size:
3423 1113 1042 636
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3222 4801 5175 5321 387
Size: 1064 138 1633 1579 374 146 1280
Fragments arranged by size:
1633 1579 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 2540 3081 3357 4277 4874 5661
Size: 393 470 438 541 276 920 597 787
Cuts at: 5661 5929 1239
Size: 268 1524
Fragments arranged by size:
1524 920 787 597 541 470 438 393
276 268
EcoRI G'AATT_C
Cuts at: 3523 3901 3523
Size: 378 5836
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3473 3473
Size: 6214
FspI TGC'GCA
Cuts at: 4720 4720
Size: 6214
HaeII r_GCGC'y
Cuts at: 1678 2052 3250 5361 1678
Size: 374 1198 2111 2531
Fragments arranged by size:
2531 2111 1198 374
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2015 2047 2765 2797 2817
Size: 32 135 32 443 32 718 32 20
Cuts at: 2817 2849 3395 3427 4855 4887 4929 4961 1373
Size: 32 546 32 1428 32 42 32 2626
Fragments arranged by size:
2626 1428 718 546 443 135 42 32
32 32 32 32 32 32 32 20
HincII GTy'rAC
Cuts at: 669 1657 1938 3369 3558 3804 669
Size: 988 281 1431 189 246 3079
Fragments arranged by size:
3079 1431 988 281 246 189
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6214
HpaI GTT'AAC
Cuts at: 3804 3804
Size: 6214
KasI G'GCGC_C
Cuts at: 2048 3246 2048
Size: 1198 5016
KpnI G_GTAC'C
Cuts at: 3434 3539 3434
Size: 105 6109
MfeI C'AATT_G
Cuts at: 2592 3813 2592
Size: 1221 4993
MluI A'CGCG_T
Cuts at: 6090 6090
Size: 6214
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1989 5328 5512 1305
Size: 684 3339 184 2007
Fragments arranged by size:
3339 2007 684 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2965 10
Size: 54 1425 343 1133 3259
Fragments arranged by size:
3259 1425 1133 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2769 3303 4172 4531 4690 518
Size: 648 1603 534 869 359 159 2042
Fragments arranged by size:
2042 1603 869 648 534 359 159
MspA1I CmG'CkG
Cuts at: 1606 2491 2843 3630 4307 5455 5700 1606
Size: 885 352 787 677 1148 245 2120
Fragments arranged by size:
2120 1148 885 787 677 352 245
NarI GG'CG_CC
Cuts at: 2049 3247 2049
Size: 1198 5016
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5662
NdeI CA'TA_TG
Cuts at: 387 3479 387
Size: 3092 3122
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6214
NotI GC'GGCC_GC
Cuts at: 3661 3661
Size: 6214
NspI r_CATG'y
Cuts at: 1132 1229 3572 5117 1132
Size: 97 2343 1545 2229
Fragments arranged by size:
2343 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6214
PciI A'CATG_T
Cuts at: 5113 5113
Size: 6214
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2924 1108
Size: 1816 4398
PfoI T'CCnGG_A
Cuts at: 2308 2308
Size: 6214
PmeI GTTT'AAAC
Cuts at: 3518 3518
Size: 6214
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4283 4315 5796 5828 4283
Size: 32 1481 32 4669
Fragments arranged by size:
4669 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6214
PsiI TTA'TAA
Cuts at: 2297 3784 2297
Size: 1487 4727
PstI C_TGCA'G
Cuts at: 830 2206 2645 3040 3566 830
Size: 1376 439 395 526 3478
Fragments arranged by size:
3478 1376 526 439 395
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6214
PvuII CAG'CTG
Cuts at: 2491 2843 2491
Size: 352 5862
SalI G'TCGA_C
Cuts at: 1936 3556 1936
Size: 1620 4594
SbfI CC_TGCA'GG
Cuts at: 3566 3566
Size: 6214
ScaI AGT'ACT
Cuts at: 4462 6174 4462
Size: 1712 4502
SfcI C'TryA_G
Cuts at: 826 1045 2202 2641 3036 3562 4697 5378 5569
Size: 219 1157 439 395 526 1135 681 191
Cuts at: 5569 826
Size: 1471
Fragments arranged by size:
1471 1157 1135 681 526 439 395 219
191
SfoI GGC'GCC
Cuts at: 2050 3248 2050
Size: 1198 5016
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6214
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6214
SmaI CCC'GGG
Cuts at: 1872 3541 1872
Size: 1669 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2734 3044 4318 5219 5481 820
Size: 197 926 791 310 1274 901 262 1553
Fragments arranged by size:
1553 1274 926 901 791 310 262 197
SnaBI TAC'GTA
Cuts at: 493 4005 493
Size: 3512 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6214
SphI G_CATG'C
Cuts at: 1132 3572 1132
Size: 2440 3774
SspI AAT'ATT
Cuts at: 5 52 2274 4138 5
Size: 47 2222 1864 2081
Fragments arranged by size:
2222 2081 1864 47
StuI AGG'CCT
Cuts at: 1517 2663 3042 1517
Size: 1146 379 4689
Fragments arranged by size:
4689 1146 379
StyI C'CwwG_G
Cuts at: 513 1065 3288 3426 3457 3653 513
Size: 552 2223 138 31 196 3074
Fragments arranged by size:
3074 2223 552 196 138 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4241 4258 4411 4596 5041 325
Size: 1255 2661 17 153 185 445 1498
Fragments arranged by size:
2661 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4460 6172 96
Size: 274 80 33 51 1443 2483 1712 138
Fragments arranged by size:
2483 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6174 6206 6174
Size: 32 6182
Tth111I GACn'n_nGTC
Cuts at: 1316 3364 1316
Size: 2048 4166
XbaI T'CTAG_A
Cuts at: 3550 3550
Size: 6214
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6214
XmaI C'CCGG_G
Cuts at: 1870 3539 1870
Size: 1669 4545
XmnI GAAnn'nnTTC
Cuts at: 2553 2568 4343 2553
Size: 15 1775 4424
Fragments arranged by size:
4424 1775 15
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4022 276
Size: 53 83 186 936 2488 2468
Fragments arranged by size:
2488 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI
XcmI