Restriction map for
pFN21AE1947
(Circular) (Six-base) MAPSORT of: pFN21AE1947.seq Check: 6118 from: 1 to: 6208
pFN21AE1947 6208 bp
With 182 enzymes: SgfI *
May 1, 2018 17:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4018 278
Size: 53 83 186 936 2482 2468
Fragments arranged by size:
2482 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2756 3551 1937
Size: 819 795 4594
Fragments arranged by size:
4594 819 795
Acc65I G'GTAC_C
Cuts at: 3529 3529
Size: 6208
AclI AA'CG_TT
Cuts at: 1563 4335 4708 1563
Size: 2772 373 3063
Fragments arranged by size:
3063 2772 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2700 3524 4271 5655 5923 1632
Size: 1068 824 747 1384 268 1917
Fragments arranged by size:
1917 1384 1068 824 747 268
AfeI AGC'GCT
Cuts at: 3259 3259
Size: 6208
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6011
AflIII A'CryG_T
Cuts at: 1203 5107 6084 1203
Size: 3904 977 1327
Fragments arranged by size:
3904 1327 977
AgeI A'CCGG_T
Cuts at: 5039 5039
Size: 6208
AhdI GACnn_n'nnGTC
Cuts at: 4937 4937
Size: 6208
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6208
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2102 1184
Size: 918 5290
AlwNI CAG_nnn'CTG
Cuts at: 2751 2824 3951 5523 2751
Size: 73 1127 1572 3436
Fragments arranged by size:
3436 1572 1127 73
ApaI G_GGCC'C
Cuts at: 3232 3232
Size: 6208
ApaLI G'TGCA_C
Cuts at: 3269 4265 5421 3269
Size: 996 1156 4056
Fragments arranged by size:
4056 1156 996
ApoI r'AATT_y
Cuts at: 1493 3517 3743 3895 6004 1493
Size: 2024 226 152 2109 1697
Fragments arranged by size:
2109 2024 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4762 160
Size: 872 3730 1606
Fragments arranged by size:
3730 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6208
AvaI C'yCGr_G
Cuts at: 1870 1943 2136 2156 3533 1870
Size: 73 193 20 1377 4545
Fragments arranged by size:
4545 1377 193 73 20
BamHI G'GATC_C
Cuts at: 3538 3905 3538
Size: 367 5841
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2066 2111 2429 2876 3005
Size: 325 217 627 279 45 318 447 129
Cuts at: 3005 3529 4984 618
Size: 524 1455 1842
Fragments arranged by size:
1842 1455 627 524 447 325 318 279
217 129 45
BanII G_rGCy'C
Cuts at: 1391 2157 2166 3232 3527 1391
Size: 766 9 1066 295 4072
Fragments arranged by size:
4072 1066 766 295 9
BbeI G_GCGC'C
Cuts at: 2115 2115
Size: 6208
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3333 928
Size: 971 1434 3803
Fragments arranged by size:
3803 1434 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4399 4433 1903
Size: 34 2462 34 3678
Fragments arranged by size:
3678 2462 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2275 4100 5310 2275
Size: 1825 1210 3173
Fragments arranged by size:
3173 1825 1210
BclI T'GATC_A
Cuts at: 1548 2709 2790 1548
Size: 1161 81 4966
Fragments arranged by size:
4966 1161 81
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2109 2175 4819
Size: 107 122 71 631 605 437 66 2644
Cuts at: 4819 136
Size: 1525
Fragments arranged by size:
2644 1525 631 605 437 122 107 71
66
BglII A'GATC_T
Cuts at: 6203 6203
Size: 6208
BlpI GC'TnA_GC
Cuts at: 3625 3625
Size: 6208
Bme1580I G_kGCm'C
Cuts at: 1790 2397 2432 3179 3232 3273 4269 5425 1790
Size: 607 35 747 53 41 996 1156 2573
Fragments arranged by size:
2573 1156 996 747 607 53 41 35
BmgBI CAC'GTC
Cuts at: 1318 3129 1318
Size: 1811 4397
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2539 3023 3939
Size: 416 53 83 399 24 1122 484 916
Cuts at: 3939 4897 442
Size: 958 1753
Fragments arranged by size:
1753 1122 958 916 484 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6208
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2606 4868 1239
Size: 1367 2262 2579
Fragments arranged by size:
2579 2262 1367
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4333 5198 5496 4333
Size: 865 298 5045
Fragments arranged by size:
5045 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2135 4871 882
Size: 617 636 2736 2219
Fragments arranged by size:
2736 2219 636 617
BsaAI yAC'GTr
Cuts at: 493 1204 2233 3999 493
Size: 711 1029 1766 2702
Fragments arranged by size:
2702 1766 1029 711
BsaBI GATnn'nnATC
Cuts at: 6202 6202
Size: 6208
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2112 4015 4397 5032
Size: 53 83 186 936 579 1903 382 635
Cuts at: 5032 275
Size: 1451
Fragments arranged by size:
1903 1451 936 635 579 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2376 4641 5039 5313 5460 5871 2376
Size: 2265 398 274 147 411 2713
Fragments arranged by size:
2713 2265 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2621 2651 1178
Size: 30 165 30 1218 30 4735
Fragments arranged by size:
4735 1218 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2174 2932 1186
Size: 988 758 4462
Fragments arranged by size:
4462 988 758
BseYI C'CCAG_C
Cuts at: 2070 2425 5411 5907 2070
Size: 355 2986 496 2371
Fragments arranged by size:
2986 2371 496 355
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5635
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2170 3658 4419 5447 1540
Size: 275 186 169 1488 761 1028 2301
Fragments arranged by size:
2301 1488 1028 761 275 186 169
BsiHKAI G_wGCw'C
Cuts at: 3273 4269 4354 5425 3273
Size: 996 85 1071 4056
Fragments arranged by size:
4056 1071 996 85
BsmI GAATG_Cn'
Cuts at: 3719 3812 3719
Size: 93 6115
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2157 2166 2397 2432 3179 3232 3273
Size: 399 367 9 231 35 747 53 41
Cuts at: 3273 3527 4269 4354 5425 1391
Size: 254 742 85 1071 2174
Fragments arranged by size:
2174 1071 747 742 399 367 254 231
85 53 41 35 9
BspEI T'CCGG_A
Cuts at: 2376 5871 2376
Size: 3495 2713
BspHI T'CATG_A
Cuts at: 3369 4097 5827 3369
Size: 728 1730 3750
Fragments arranged by size:
3750 1730 728
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3111 3563 844
Size: 2267 452 3489
Fragments arranged by size:
3489 2267 452
BsrBI CCG'CTC
Cuts at: 1394 1823 4095 1394
Size: 429 2272 3507
Fragments arranged by size:
3507 2272 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2481 4703 4877 65
Size: 1183 1233 2222 174 1396
Fragments arranged by size:
2222 1396 1233 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4852 5039 1700
Size: 110 3042 187 2869
Fragments arranged by size:
3042 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2736 96
Size: 2640 3568
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6208
BssSI C'ACGA_G
Cuts at: 4268 5280 4268
Size: 1012 5196
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2309 3103 794
Size: 1515 794 3899
Fragments arranged by size:
3899 1515 794
BstBI TT'CG_AA
Cuts at: 5012 5012
Size: 6208
BstEII G'GTnAC_C
Cuts at: 1184 3058 1184
Size: 1874 4334
BstYI r'GATC_y
Cuts at: 1972 2821 3538 3905 4290 4307 5759 5845 5857
Size: 849 717 367 385 17 1452 86 12
Cuts at: 5857 6203 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 849 717 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2757 2757
Size: 6208
Bsu36I CC'TnA_GG
Cuts at: 2204 2204
Size: 6208
BtgI C'CryG_G
Cuts at: 513 1065 2004 2062 2244 2452 2946 3171 513
Size: 552 939 58 182 208 494 225 3550
Fragments arranged by size:
3550 939 552 494 225 208 182 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2318 487
Size: 683 56 1092 4377
Fragments arranged by size:
4377 1092 683 56
BtsI GCAGTG_nn'
Cuts at: 2310 3724 4518 4538 2310
Size: 1414 794 20 3980
Fragments arranged by size:
3980 1414 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6208
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6173
DraI TTT'AAA
Cuts at: 3512 3512
Size: 6208
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3358 5215 809
Size: 507 2042 1857 1802
Fragments arranged by size:
2042 1857 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2167 3107 3123
Size: 54 1425 50 275 18 337 940 16
Cuts at: 3123 3655 4544 6173 8
Size: 532 889 1629 43
Fragments arranged by size:
1629 1425 940 889 532 337 275 54
50 43 18 16
EagI C'GGCC_G
Cuts at: 1537 1812 2167 3655 1537
Size: 275 355 1488 4090
Fragments arranged by size:
4090 1488 355 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2145 2639 3413 4138 1353
Size: 792 494 774 725 3423
Fragments arranged by size:
3423 792 774 725 494
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4795 5169 5315 387
Size: 1064 138 3206 374 146 1280
Fragments arranged by size:
3206 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2606 2700 3524 4271 4868 5655 5923
Size: 393 974 94 824 747 597 787 268
Cuts at: 5923 1239
Size: 1524
Fragments arranged by size:
1524 974 824 787 747 597 393 268
94
EcoNI CCTnn'n_nnAGG
Cuts at: 2378 3503 2378
Size: 1125 5083
EcoO109I rG'GnC_Cy
Cuts at: 2371 3302 2371
Size: 931 5277
EcoRI G'AATT_C
Cuts at: 3517 3895 3517
Size: 378 5830
FspI TGC'GCA
Cuts at: 2096 4714 2096
Size: 2618 3590
HaeII r_GCGC'y
Cuts at: 1678 2115 3261 3448 5355 1678
Size: 437 1146 187 1907 2531
Fragments arranged by size:
2531 1907 1146 437 187
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3180 3212 3479 3511 4849
Size: 32 135 32 1608 32 267 32 1338
Cuts at: 4849 4881 4923 4955 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1608 1338 267 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3363 3552 3798 669
Size: 988 281 1425 189 246 3079
Fragments arranged by size:
3079 1425 988 281 246 189
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6208
HpaI GTT'AAC
Cuts at: 3798 3798
Size: 6208
KasI G'GCGC_C
Cuts at: 2111 2111
Size: 6208
KpnI G_GTAC'C
Cuts at: 3533 3533
Size: 6208
MfeI C'AATT_G
Cuts at: 3807 3807
Size: 6208
MluI A'CGCG_T
Cuts at: 6084 6084
Size: 6208
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2550 2677 5322 5506 1305
Size: 1245 127 2645 184 2007
Fragments arranged by size:
2645 2007 1245 184 127
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3125 10
Size: 54 1425 343 1293 3093
Fragments arranged by size:
3093 1425 1293 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2276 3182 4166 4525 4684 518
Size: 648 1110 906 984 359 159 2042
Fragments arranged by size:
2042 1110 984 906 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2100 2353 2986 3293 3456 3624 4301 5449
Size: 494 253 633 307 163 168 677 1148
Cuts at: 5449 5694 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 633 494 307 253 245
168 163
NarI GG'CG_CC
Cuts at: 2112 2112
Size: 6208
NcoI C'CATG_G
Cuts at: 513 1065 2004 2452 513
Size: 552 939 448 4269
Fragments arranged by size:
4269 939 552 448
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6208
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6208
NotI GC'GGCC_GC
Cuts at: 3655 3655
Size: 6208
NspI r_CATG'y
Cuts at: 1132 1229 3566 5111 1132
Size: 97 2337 1545 2229
Fragments arranged by size:
2337 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2119 2404 1348
Size: 771 285 5152
Fragments arranged by size:
5152 771 285
PciI A'CATG_T
Cuts at: 5107 5107
Size: 6208
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3015 1108
Size: 1907 4301
PmeI GTTT'AAAC
Cuts at: 3512 3512
Size: 6208
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4277 4309 5790 5822 4277
Size: 32 1481 32 4663
Fragments arranged by size:
4663 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2371 2371
Size: 6208
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6208
PsiI TTA'TAA
Cuts at: 3778 3778
Size: 6208
PspOMI G'GGCC_C
Cuts at: 3228 3228
Size: 6208
PstI C_TGCA'G
Cuts at: 830 2646 2920 3560 830
Size: 1816 274 640 3478
Fragments arranged by size:
3478 1816 640 274
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6208
PvuII CAG'CTG
Cuts at: 2353 2986 2353
Size: 633 5575
SalI G'TCGA_C
Cuts at: 1936 3550 1936
Size: 1614 4594
SapI GCTCTTCn'nnn_
Cuts at: 2145 2145
Size: 6208
SbfI CC_TGCA'GG
Cuts at: 3560 3560
Size: 6208
ScaI AGT'ACT
Cuts at: 4456 6168 4456
Size: 1712 4496
SexAI A'CCwGG_T
Cuts at: 2436 2436
Size: 6208
SfcI C'TryA_G
Cuts at: 826 1045 2642 2916 3556 4691 5372 5563 826
Size: 219 1597 274 640 1135 681 191 1471
Fragments arranged by size:
1597 1471 1135 681 640 274 219 191
SfoI GGC'GCC
Cuts at: 2113 2113
Size: 6208
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6208
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6208
SmaI CCC'GGG
Cuts at: 1872 2138 3535 1872
Size: 266 1397 4545
Fragments arranged by size:
4545 1397 266
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4312 5213 5475 820
Size: 197 926 2369 901 262 1553
Fragments arranged by size:
2369 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3999 493
Size: 3506 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6208
SphI G_CATG'C
Cuts at: 1132 3566 1132
Size: 2434 3774
SspI AAT'ATT
Cuts at: 5 52 4132 5
Size: 47 4080 2081
Fragments arranged by size:
4080 2081 47
StuI AGG'CCT
Cuts at: 1517 2572 1517
Size: 1055 5153
StyI C'CwwG_G
Cuts at: 513 1065 2004 2452 3014 3315 3647 513
Size: 552 939 448 562 301 332 3074
Fragments arranged by size:
3074 939 562 552 448 332 301
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2084 2159 2297 2417 2776 3041 4235
Size: 1255 504 75 138 120 359 265 1194
Cuts at: 4235 4252 4405 4590 5035 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1194 504 445 359 265 185
153 138 120 75 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2736 2844 4454
Size: 274 80 33 51 1443 759 108 1610
Cuts at: 4454 6166 96
Size: 1712 138
Fragments arranged by size:
1712 1610 1443 759 274 138 108 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3073 3105 6168 6200 3073
Size: 32 3063 32 3081
Fragments arranged by size:
3081 3063 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6208
XbaI T'CTAG_A
Cuts at: 3544 3544
Size: 6208
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6208
XmaI C'CCGG_G
Cuts at: 1870 2136 3533 1870
Size: 266 1397 4545
Fragments arranged by size:
4545 1397 266
XmnI GAAnn'nnTTC
Cuts at: 3023 4337 3023
Size: 1314 4894
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4016 276
Size: 53 83 186 936 2482 2468
Fragments arranged by size:
2482 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI DraIII EcoICRI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI
PmlI PspXI PsrI RsrII SacI SacII SanDI SfiI
SrfI SwaI XcmI