Restriction map for
pFN21AE1942
(Circular) (Six-base) MAPSORT of: pFN21AE1942.seq Check: 9130 from: 1 to: 6193
pFN21AE1942 6193 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2701 2750 2701
Size: 49 6144
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4003 278
Size: 53 83 186 936 2467 2468
Fragments arranged by size:
2468 2467 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2142 3536 1937
Size: 205 1394 4594
Fragments arranged by size:
4594 1394 205
Acc65I G'GTAC_C
Cuts at: 3514 3514
Size: 6193
AclI AA'CG_TT
Cuts at: 1563 4320 4693 1563
Size: 2757 373 3063
Fragments arranged by size:
3063 2757 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4256 5640 5908 1632
Size: 2624 1384 268 1917
Fragments arranged by size:
2624 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5996
AflIII A'CryG_T
Cuts at: 1203 2493 2599 2922 5092 6069 1203
Size: 1290 106 323 2170 977 1327
Fragments arranged by size:
2170 1327 1290 977 323 106
AgeI A'CCGG_T
Cuts at: 5024 5024
Size: 6193
AhdI GACnn_n'nnGTC
Cuts at: 4922 4922
Size: 6193
AleI CACnn'nnGTG
Cuts at: 1166 2240 1166
Size: 1074 5119
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2063 3359 1184
Size: 879 1296 4018
Fragments arranged by size:
4018 1296 879
AlwNI CAG_nnn'CTG
Cuts at: 2065 2344 3281 3433 3936 5508 2065
Size: 279 937 152 503 1572 2750
Fragments arranged by size:
2750 1572 937 503 279 152
ApaI G_GGCC'C
Cuts at: 2229 2509 3133 2229
Size: 280 624 5289
Fragments arranged by size:
5289 624 280
ApaLI G'TGCA_C
Cuts at: 4250 5406 4250
Size: 1156 5037
ApoI r'AATT_y
Cuts at: 1493 2171 3398 3502 3728 3880 5989 1493
Size: 678 1227 104 226 152 2109 1697
Fragments arranged by size:
2109 1697 1227 678 226 152 104
AseI AT'TA_AT
Cuts at: 160 1032 3180 4747 160
Size: 872 2148 1567 1606
Fragments arranged by size:
2148 1606 1567 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6193
AvaI C'yCGr_G
Cuts at: 1870 1943 2238 2508 3125 3518 1870
Size: 73 295 270 617 393 4545
Fragments arranged by size:
4545 617 393 295 270 73
BamHI G'GATC_C
Cuts at: 2849 3523 3890 2849
Size: 674 367 5152
Fragments arranged by size:
5152 674 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2354 3514 4969 618
Size: 325 217 627 567 1160 1455 1842
Fragments arranged by size:
1842 1455 1160 627 567 325 217
BanII G_rGCy'C
Cuts at: 1391 2229 2296 2509 3133 3512 1391
Size: 838 67 213 624 379 4072
Fragments arranged by size:
4072 838 624 379 213 67
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2827 3142 3375 928
Size: 971 928 315 233 3746
Fragments arranged by size:
3746 971 928 315 233
BbvCI CC'TCA_GC
Cuts at: 3260 3260
Size: 6193
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4384 4418 1903
Size: 34 2447 34 3678
Fragments arranged by size:
3678 2447 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2345 2905 4085 5295 2345
Size: 560 1180 1210 3243
Fragments arranged by size:
3243 1210 1180 560
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6193
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2757 3197 4804
Size: 107 122 71 631 605 1085 440 1607
Cuts at: 4804 136
Size: 1525
Fragments arranged by size:
1607 1525 1085 631 605 440 122 107
71
BglII A'GATC_T
Cuts at: 3354 6188 3354
Size: 2834 3359
BlpI GC'TnA_GC
Cuts at: 3610 3610
Size: 6193
Bme1580I G_kGCm'C
Cuts at: 1790 2229 2509 3059 3133 4254 5410 1790
Size: 439 280 550 74 1121 1156 2573
Fragments arranged by size:
2573 1156 1121 550 439 280 74
BmgBI CAC'GTC
Cuts at: 1318 2221 2494 2602 2923 1318
Size: 903 273 108 321 4588
Fragments arranged by size:
4588 903 321 273 108
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2083 3075 3924
Size: 416 53 83 399 24 666 992 849
Cuts at: 3924 4882 442
Size: 958 1753
Fragments arranged by size:
1753 992 958 849 666 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6193
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2120 2120
Size: 6193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2042 3497 4853 1239
Size: 803 1455 1356 2579
Fragments arranged by size:
2579 1455 1356 803
Bpu10I CC'TnA_GC
Cuts at: 3260 3260
Size: 6193
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2947 3062 4318 5183 5481 2947
Size: 115 1256 865 298 3659
Fragments arranged by size:
3659 1256 865 298 115
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4856 882
Size: 617 3357 2219
Fragments arranged by size:
3357 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2611 3984 493
Size: 711 1407 1373 2702
Fragments arranged by size:
2702 1407 1373 711
BsaBI GATnn'nnATC
Cuts at: 6187 6187
Size: 6193
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2520 4000 4382 5017
Size: 53 83 186 936 987 1480 382 635
Cuts at: 5017 275
Size: 1451
Fragments arranged by size:
1480 1451 987 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4626 5024 5298 5445 5856 4626
Size: 398 274 147 411 4963
Fragments arranged by size:
4963 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5968
Fragments arranged by size:
5968 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6193
BseYI C'CCAG_C
Cuts at: 5396 5892 5396
Size: 496 5697
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2089 2507 2975 1163
Size: 573 353 418 468 4381
Fragments arranged by size:
4381 573 468 418 353
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3643 4404 5432 1540
Size: 275 186 1642 761 1028 2301
Fragments arranged by size:
2301 1642 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3294 4254 4339 5410 3294
Size: 960 85 1071 4077
Fragments arranged by size:
4077 1071 960 85
BsmI GAATG_Cn'
Cuts at: 3704 3797 3704
Size: 93 6100
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2229 2296 2509 3059 3133 3294 3512
Size: 399 439 67 213 550 74 161 218
Cuts at: 3512 4254 4339 5410 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 550 439 399 218 213
161 85 74 67
BspEI T'CCGG_A
Cuts at: 5856 5856
Size: 6193
BspHI T'CATG_A
Cuts at: 4082 5812 4082
Size: 1730 4463
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2701 2750 2795 3548 844
Size: 1857 49 45 753 3489
Fragments arranged by size:
3489 1857 753 49 45
BsrBI CCG'CTC
Cuts at: 1394 1823 4080 1394
Size: 429 2257 3507
Fragments arranged by size:
3507 2257 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2797 3247 4688 4862 65
Size: 1183 1549 450 1441 174 1396
Fragments arranged by size:
1549 1441 1396 1183 450 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2363 4837 5024 1700
Size: 110 553 2474 187 2869
Fragments arranged by size:
2869 2474 553 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6193
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6193
BssSI C'ACGA_G
Cuts at: 2753 4253 5265 2753
Size: 1500 1012 3681
Fragments arranged by size:
3681 1500 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2326 2787 2796 794
Size: 1532 461 9 4191
Fragments arranged by size:
4191 1532 461 9
BstBI TT'CG_AA
Cuts at: 4997 4997
Size: 6193
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6193
BstXI CCAn_nnnn'nTGG
Cuts at: 2128 2242 2542 2830 3445 2128
Size: 114 300 288 615 4876
Fragments arranged by size:
4876 615 300 288 114
BstYI r'GATC_y
Cuts at: 1972 2849 3354 3523 3890 4275 4292 5744 5830
Size: 877 505 169 367 385 17 1452 86
Cuts at: 5830 5842 6188 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 877 505 385 367 346 169
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3246 513
Size: 552 2181 3460
Fragments arranged by size:
3460 2181 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2418 487
Size: 683 56 1192 4262
Fragments arranged by size:
4262 1192 683 56
BtsI GCAGTG_nn'
Cuts at: 2312 2806 3709 4503 4523 2312
Size: 494 903 794 20 3982
Fragments arranged by size:
3982 903 794 494 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6193
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6158
DraI TTT'AAA
Cuts at: 3497 3497
Size: 6193
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2600 5200 809
Size: 507 1284 2600 1802
Fragments arranged by size:
2600 1802 1284 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2370 2820 3160
Size: 54 1425 50 275 18 540 450 340
Cuts at: 3160 3468 3640 4529 6158 8
Size: 308 172 889 1629 43
Fragments arranged by size:
1629 1425 889 540 450 340 308 275
172 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3640 1537
Size: 275 1828 4090
Fragments arranged by size:
4090 1828 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4123 1353
Size: 2770 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4780 5154 5300 387
Size: 1064 138 3191 374 146 1280
Fragments arranged by size:
3191 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 3497 4256 4853 5640 5908 1239
Size: 393 410 1455 759 597 787 268 1524
Fragments arranged by size:
1524 1455 787 759 597 410 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2198 2198
Size: 6193
EcoO109I rG'GnC_Cy
Cuts at: 2011 2062 2225 2427 2472 3129 3430 2011
Size: 51 163 202 45 657 301 4774
Fragments arranged by size:
4774 657 301 202 163 51 45
EcoRI G'AATT_C
Cuts at: 3502 3880 3502
Size: 378 5815
FspI TGC'GCA
Cuts at: 4699 4699
Size: 6193
HaeII r_GCGC'y
Cuts at: 1678 5340 1678
Size: 3662 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2624 2656 4834 4866 4908
Size: 32 135 32 1052 32 2178 32 42
Cuts at: 4908 4940 1373
Size: 32 2626
Fragments arranged by size:
2626 2178 1052 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2143 2254 3537 3783 669
Size: 988 281 205 111 1283 246 3079
Fragments arranged by size:
3079 1283 988 281 246 205 111
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6193
HpaI GTT'AAC
Cuts at: 3783 3783
Size: 6193
KpnI G_GTAC'C
Cuts at: 3518 3518
Size: 6193
MfeI C'AATT_G
Cuts at: 3792 3792
Size: 6193
MluI A'CGCG_T
Cuts at: 6069 6069
Size: 6193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5307 5491 1305
Size: 4002 184 2007
Fragments arranged by size:
4002 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2372 3470 10
Size: 54 1425 343 540 1098 2733
Fragments arranged by size:
2733 1425 1098 540 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2240 2456 2540 2703 4151 4510 4669
Size: 648 1074 216 84 163 1448 359 159
Cuts at: 4669 518
Size: 2042
Fragments arranged by size:
2042 1448 1074 648 359 216 163 159
84
MspA1I CmG'CkG
Cuts at: 1606 2344 2517 3609 4286 5434 5679 1606
Size: 738 173 1092 677 1148 245 2120
Fragments arranged by size:
2120 1148 1092 738 677 245 173
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5641
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6193
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6193
NotI GC'GGCC_GC
Cuts at: 3640 3640
Size: 6193
NspI r_CATG'y
Cuts at: 1132 1229 3551 5096 1132
Size: 97 2322 1545 2229
Fragments arranged by size:
2322 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2989 1348
Size: 1641 4552
PciI A'CATG_T
Cuts at: 5092 5092
Size: 6193
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3139 1108
Size: 2031 4162
PfoI T'CCnGG_A
Cuts at: 2642 2652 3474 2642
Size: 10 822 5361
Fragments arranged by size:
5361 822 10
PmeI GTTT'AAAC
Cuts at: 3497 3497
Size: 6193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4262 4294 5775 5807 4262
Size: 32 1481 32 4648
Fragments arranged by size:
4648 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2011 2427 2011
Size: 416 5777
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6193
PsiI TTA'TAA
Cuts at: 3763 3763
Size: 6193
PspOMI G'GGCC_C
Cuts at: 2225 2505 3129 2225
Size: 280 624 5289
Fragments arranged by size:
5289 624 280
PstI C_TGCA'G
Cuts at: 830 2042 2322 2801 3429 3545 830
Size: 1212 280 479 628 116 3478
Fragments arranged by size:
3478 1212 628 479 280 116
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6193
SalI G'TCGA_C
Cuts at: 1936 2141 3535 1936
Size: 205 1394 4594
Fragments arranged by size:
4594 1394 205
SbfI CC_TGCA'GG
Cuts at: 3545 3545
Size: 6193
ScaI AGT'ACT
Cuts at: 2960 4441 6153 2960
Size: 1481 1712 3000
Fragments arranged by size:
3000 1712 1481
SexAI A'CCwGG_T
Cuts at: 2127 2829 2127
Size: 702 5491
SfcI C'TryA_G
Cuts at: 826 1045 2038 2318 2620 2797 3425 3541 4676
Size: 219 993 280 302 177 628 116 1135
Cuts at: 4676 5357 5548 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 993 681 628 302 280 219
191 177 116
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6193
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6193
SmaI CCC'GGG
Cuts at: 1872 2240 2510 3127 3520 1872
Size: 368 270 617 393 4545
Fragments arranged by size:
4545 617 393 368 270
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2962 3077 4297 5198 5460 820
Size: 197 926 1019 115 1220 901 262 1553
Fragments arranged by size:
1553 1220 1019 926 901 262 197 115
SnaBI TAC'GTA
Cuts at: 493 3984 493
Size: 3491 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6193
SphI G_CATG'C
Cuts at: 1132 3551 1132
Size: 2419 3774
SrfI GCCC'GGGC
Cuts at: 2510 2510
Size: 6193
SspI AAT'ATT
Cuts at: 5 52 4117 5
Size: 47 4065 2081
Fragments arranged by size:
4065 2081 47
StuI AGG'CCT
Cuts at: 1517 2660 1517
Size: 1143 5050
StyI C'CwwG_G
Cuts at: 513 1065 2304 3632 513
Size: 552 1239 1328 3074
Fragments arranged by size:
3074 1328 1239 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3221 4220 4237 4390 4575 5020 325
Size: 1255 1641 999 17 153 185 445 1498
Fragments arranged by size:
1641 1498 1255 999 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2958 4439 6151
Size: 274 80 33 51 1443 981 1481 1712
Cuts at: 6151 96
Size: 138
Fragments arranged by size:
1712 1481 1443 981 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3359 3391 6153 6185 3359
Size: 32 2762 32 3367
Fragments arranged by size:
3367 2762 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2831 1316
Size: 1515 4678
XbaI T'CTAG_A
Cuts at: 3529 3529
Size: 6193
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2365 2365
Size: 6193
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6193
XmaI C'CCGG_G
Cuts at: 1870 2238 2508 3125 3518 1870
Size: 368 270 617 393 4545
Fragments arranged by size:
4545 617 393 368 270
XmnI GAAnn'nnTTC
Cuts at: 2189 2891 4322 2189
Size: 702 1431 4060
Fragments arranged by size:
4060 1431 702
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4001 276
Size: 53 83 186 936 2467 2468
Fragments arranged by size:
2468 2467 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SfiI SfoI SwaI