Restriction map for
pFN21AE1940
(Circular) (Six-base) MAPSORT of: pFN21AE1940.seq Check: 3029 from: 1 to: 6163
pFN21AE1940 6163 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2660 2660
Size: 6163
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3973 278
Size: 53 83 186 936 2437 2468
Fragments arranged by size:
2468 2437 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2775 3506 1937
Size: 838 731 4594
Fragments arranged by size:
4594 838 731
Acc65I G'GTAC_C
Cuts at: 3484 3484
Size: 6163
AclI AA'CG_TT
Cuts at: 1563 4290 4663 1563
Size: 2727 373 3063
Fragments arranged by size:
3063 2727 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2933 3143 3178 3309 4226 5610 5878 1632
Size: 1301 210 35 131 917 1384 268 1917
Fragments arranged by size:
1917 1384 1301 917 268 210 131 35
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5966
AflIII A'CryG_T
Cuts at: 1203 3096 5062 6039 1203
Size: 1893 1966 977 1327
Fragments arranged by size:
1966 1893 1327 977
AgeI A'CCGG_T
Cuts at: 4994 4994
Size: 6163
AhdI GACnn_n'nnGTC
Cuts at: 4892 4892
Size: 6163
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6163
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6163
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3236 3268 3236
Size: 32 6131
AlwNI CAG_nnn'CTG
Cuts at: 2218 2798 2911 3088 3279 3319 3906 5478 2218
Size: 580 113 177 191 40 587 1572 2903
Fragments arranged by size:
2903 1572 587 580 191 177 113 40
ApaI G_GGCC'C
Cuts at: 2239 2815 2239
Size: 576 5587
ApaLI G'TGCA_C
Cuts at: 4220 5376 4220
Size: 1156 5007
ApoI r'AATT_y
Cuts at: 1493 3074 3472 3698 3850 5959 1493
Size: 1581 398 226 152 2109 1697
Fragments arranged by size:
2109 1697 1581 398 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4717 160
Size: 872 3685 1606
Fragments arranged by size:
3685 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6163
AvaI C'yCGr_G
Cuts at: 1870 1943 2021 2859 3488 1870
Size: 73 78 838 629 4545
Fragments arranged by size:
4545 838 629 78 73
BamHI G'GATC_C
Cuts at: 3029 3493 3860 3029
Size: 464 367 5332
Fragments arranged by size:
5332 464 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2144 2171 2387 2830 3484
Size: 325 217 627 357 27 216 443 654
Cuts at: 3484 4939 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 654 627 443 357 325 217
216 27
BanII G_rGCy'C
Cuts at: 1391 2131 2158 2239 2678 2815 3482 1391
Size: 740 27 81 439 137 667 4072
Fragments arranged by size:
4072 740 667 439 137 81 27
BbeI G_GCGC'C
Cuts at: 2148 2391 2148
Size: 243 5920
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3099 3184 928
Size: 971 1200 85 3907
Fragments arranged by size:
3907 1200 971 85
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4354 4388 1903
Size: 34 50 34 2333 34 3678
Fragments arranged by size:
3678 2333 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4055 5265 4055
Size: 1210 4953
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6163
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2385 4774 136
Size: 107 122 71 631 605 713 2389 1525
Fragments arranged by size:
2389 1525 713 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6158 6158
Size: 6163
BlpI GC'TnA_GC
Cuts at: 3269 3580 3269
Size: 311 5852
Bme1580I G_kGCm'C
Cuts at: 1790 2174 2239 2815 4224 5380 1790
Size: 384 65 576 1409 1156 2573
Fragments arranged by size:
2573 1409 1156 576 384 65
BmgBI CAC'GTC
Cuts at: 1318 3097 3299 1318
Size: 1779 202 4182
Fragments arranged by size:
4182 1779 202
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2386 3410 3437
Size: 416 53 83 399 24 969 1024 27
Cuts at: 3437 3894 4852 442
Size: 457 958 1753
Fragments arranged by size:
1753 1024 969 958 457 416 399 83
53 27 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6163
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2396 2396
Size: 6163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2069 2474 2522 3163 4823 1239
Size: 830 405 48 641 1660 2579
Fragments arranged by size:
2579 1660 830 641 405 48
Bpu10I CC'TnA_GC
Cuts at: 2682 2682
Size: 6163
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4288 5153 5451 4288
Size: 865 298 5000
Fragments arranged by size:
5000 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4826 882
Size: 617 3327 2219
Fragments arranged by size:
3327 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2606 3954 493
Size: 711 1402 1348 2702
Fragments arranged by size:
2702 1402 1348 711
BsaBI GATnn'nnATC
Cuts at: 6157 6157
Size: 6163
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2145 2388 2688 2928
Size: 53 83 186 936 612 243 300 240
Cuts at: 2928 3970 4352 4987 275
Size: 1042 382 635 1451
Fragments arranged by size:
1451 1042 936 635 612 382 300 243
240 186 83 53
BsaWI w'CCGG_w
Cuts at: 4596 4994 5268 5415 5826 4596
Size: 398 274 147 411 4933
Fragments arranged by size:
4933 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2164 2194 3236 3266 1178
Size: 30 165 30 761 30 1042 30 4075
Fragments arranged by size:
4075 1042 761 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3137 1186
Size: 1951 4212
BseYI C'CCAG_C
Cuts at: 2140 2204 2620 5366 5862 2140
Size: 64 416 2746 496 2441
Fragments arranged by size:
2746 2441 496 416 64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5590
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3613 4374 5402 1540
Size: 275 186 1612 761 1028 2301
Fragments arranged by size:
2301 1612 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2678 2881 4224 4309 5380 2678
Size: 203 1343 85 1071 3461
Fragments arranged by size:
3461 1343 1071 203 85
BsmI GAATG_Cn'
Cuts at: 3674 3767 3674
Size: 93 6070
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2131 2158 2174 2239 2678 2815 2881
Size: 399 341 27 16 65 439 137 66
Cuts at: 2881 3482 4224 4309 5380 1391
Size: 601 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 601 439 399 341 137
85 66 65 27 16
BspEI T'CCGG_A
Cuts at: 5826 5826
Size: 6163
BspHI T'CATG_A
Cuts at: 4052 5782 4052
Size: 1730 4433
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2286 2287 2337 2660 3189 3518 844
Size: 1442 1 50 323 529 329 3489
Fragments arranged by size:
3489 1442 529 329 323 50 1
BsrBI CCG'CTC
Cuts at: 1394 1823 2257 4050 1394
Size: 429 434 1793 3507
Fragments arranged by size:
3507 1793 434 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4658 4832 65
Size: 1183 3410 174 1396
Fragments arranged by size:
3410 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3068 4807 4994 1700
Size: 110 1258 1739 187 2869
Fragments arranged by size:
2869 1739 1258 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6163
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6163
BssSI C'ACGA_G
Cuts at: 2258 2381 4223 5235 2258
Size: 123 1842 1012 3186
Fragments arranged by size:
3186 1842 1012 123
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3279 794
Size: 2485 3678
BstBI TT'CG_AA
Cuts at: 4967 4967
Size: 6163
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6163
BstXI CCAn_nnnn'nTGG
Cuts at: 3381 3381
Size: 6163
BstYI r'GATC_y
Cuts at: 1972 3029 3493 3860 4245 4262 5714 5800 5812
Size: 1057 464 367 385 17 1452 86 12
Cuts at: 5812 6158 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1057 464 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2291 3081 2291
Size: 790 5373
BtgI C'CryG_G
Cuts at: 513 1065 2949 513
Size: 552 1884 3727
Fragments arranged by size:
3727 1884 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5424
Fragments arranged by size:
5424 683 56
BtsI GCAGTG_nn'
Cuts at: 3679 4473 4493 3679
Size: 794 20 5349
Fragments arranged by size:
5349 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6163
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6128
DraI TTT'AAA
Cuts at: 3467 3467
Size: 6163
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5170 809
Size: 507 3854 1802
Fragments arranged by size:
3854 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2266 2347 2572
Size: 54 1425 50 275 18 436 81 225
Cuts at: 2572 3432 3610 4499 6128 8
Size: 860 178 889 1629 43
Fragments arranged by size:
1629 1425 889 860 436 275 225 178
81 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3610 1537
Size: 275 1798 4090
Fragments arranged by size:
4090 1798 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3147 4093 1353
Size: 1794 946 3423
Fragments arranged by size:
3423 1794 946
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4750 5124 5270 387
Size: 1064 138 3161 374 146 1280
Fragments arranged by size:
3161 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2676 2676
Size: 6163
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2474 2522 2933 3143 3163 3178
Size: 393 437 405 48 411 210 20 15
Cuts at: 3178 3309 4226 4823 5610 5878 1239
Size: 131 917 597 787 268 1524
Fragments arranged by size:
1524 917 787 597 437 411 405 393
268 210 131 48 20 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2294 3119 2294
Size: 825 5338
EcoO109I rG'GnC_Cy
Cuts at: 2027 2117 2215 2236 2812 2027
Size: 90 98 21 576 5378
Fragments arranged by size:
5378 576 98 90 21
EcoRI G'AATT_C
Cuts at: 3472 3850 3472
Size: 378 5785
FspI TGC'GCA
Cuts at: 2333 4669 2333
Size: 2336 3827
HaeII r_GCGC'y
Cuts at: 1678 2148 2391 5310 1678
Size: 470 243 2919 2531
Fragments arranged by size:
2919 2531 470 243
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3301 3333 4804 4836 4878
Size: 32 135 32 1729 32 1471 32 42
Cuts at: 4878 4910 1373
Size: 32 2626
Fragments arranged by size:
2626 1729 1471 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2326 3507 3753 669
Size: 988 281 388 1181 246 3079
Fragments arranged by size:
3079 1181 988 388 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6163
HpaI GTT'AAC
Cuts at: 2326 3753 2326
Size: 1427 4736
KasI G'GCGC_C
Cuts at: 2144 2387 2144
Size: 243 5920
KpnI G_GTAC'C
Cuts at: 3488 3488
Size: 6163
MfeI C'AATT_G
Cuts at: 3762 3762
Size: 6163
MluI A'CGCG_T
Cuts at: 6039 6039
Size: 6163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3312 5277 5461 1305
Size: 2007 1965 184 2007
Fragments arranged by size:
2007 2007 1965 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2574 3434 10
Size: 54 1425 343 742 860 2739
Fragments arranged by size:
2739 1425 860 742 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4121 4480 4639 518
Size: 648 2955 359 159 2042
Fragments arranged by size:
2955 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2715 3268 3579 4256 5404 5649 1606
Size: 1109 553 311 677 1148 245 2120
Fragments arranged by size:
2120 1148 1109 677 553 311 245
NaeI GCC'GGC
Cuts at: 3070 3070
Size: 6163
NarI GG'CG_CC
Cuts at: 2145 2388 2145
Size: 243 5920
NcoI C'CATG_G
Cuts at: 513 1065 2949 513
Size: 552 1884 3727
Fragments arranged by size:
3727 1884 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6163
NgoMIV G'CCGG_C
Cuts at: 3068 3068
Size: 6163
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6163
NotI GC'GGCC_GC
Cuts at: 3610 3610
Size: 6163
NspI r_CATG'y
Cuts at: 1132 1229 2482 2620 3521 5066 1132
Size: 97 1253 138 901 1545 2229
Fragments arranged by size:
2229 1545 1253 901 138 97
PasI CC'CwG_GG
Cuts at: 1348 2212 2664 1348
Size: 864 452 4847
Fragments arranged by size:
4847 864 452
PciI A'CATG_T
Cuts at: 5062 5062
Size: 6163
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2581 3357 1108
Size: 1473 776 3914
Fragments arranged by size:
3914 1473 776
PfoI T'CCnGG_A
Cuts at: 3255 3255
Size: 6163
PmeI GTTT'AAAC
Cuts at: 3467 3467
Size: 6163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3236 3268 4232 4264 5745 5777 3236
Size: 32 964 32 1481 32 3622
Fragments arranged by size:
3622 1481 964 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2027 2027
Size: 6163
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6163
PsiI TTA'TAA
Cuts at: 3733 3733
Size: 6163
PspOMI G'GGCC_C
Cuts at: 2235 2811 2235
Size: 576 5587
PspXI vC'TCGA_Gb
Cuts at: 2021 2021
Size: 6163
PstI C_TGCA'G
Cuts at: 830 2907 3515 830
Size: 2077 608 3478
Fragments arranged by size:
3478 2077 608
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6163
PvuII CAG'CTG
Cuts at: 3268 3268
Size: 6163
SacI G_AGCT'C
Cuts at: 2678 2678
Size: 6163
SalI G'TCGA_C
Cuts at: 1936 3505 1936
Size: 1569 4594
SanDI GG'GwC_CC
Cuts at: 2027 2027
Size: 6163
SapI GCTCTTCn'nnn_
Cuts at: 3147 3147
Size: 6163
SbfI CC_TGCA'GG
Cuts at: 3515 3515
Size: 6163
ScaI AGT'ACT
Cuts at: 4411 6123 4411
Size: 1712 4451
SfcI C'TryA_G
Cuts at: 826 1045 2315 2903 3511 4646 5327 5518 826
Size: 219 1270 588 608 1135 681 191 1471
Fragments arranged by size:
1471 1270 1135 681 608 588 219 191
SfoI GGC'GCC
Cuts at: 2146 2389 2146
Size: 243 5920
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6163
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6163
SmaI CCC'GGG
Cuts at: 1872 2861 3490 1872
Size: 989 629 4545
Fragments arranged by size:
4545 989 629
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2021 4267 5168 5430 820
Size: 197 926 78 2246 901 262 1553
Fragments arranged by size:
2246 1553 926 901 262 197 78
SnaBI TAC'GTA
Cuts at: 493 2606 3954 493
Size: 2113 1348 2702
Fragments arranged by size:
2702 2113 1348
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6163
SphI G_CATG'C
Cuts at: 1132 3521 1132
Size: 2389 3774
SspI AAT'ATT
Cuts at: 5 52 4087 5
Size: 47 4035 2081
Fragments arranged by size:
4035 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6163
StyI C'CwwG_G
Cuts at: 513 1065 2949 3240 3602 513
Size: 552 1884 291 362 3074
Fragments arranged by size:
3074 1884 552 362 291
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2225 2660 4190 4207 4360 4545 4990
Size: 1255 645 435 1530 17 153 185 445
Cuts at: 4990 325
Size: 1498
Fragments arranged by size:
1530 1498 1255 645 445 435 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4409 6121 96
Size: 274 80 33 51 1443 2432 1712 138
Fragments arranged by size:
2432 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2164 2196 3331 3363 6123 6155 2164
Size: 32 1135 32 2760 32 2172
Fragments arranged by size:
2760 2172 1135 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3315 1316
Size: 1999 4164
XbaI T'CTAG_A
Cuts at: 3499 3499
Size: 6163
XhoI C'TCGA_G
Cuts at: 1943 2021 1943
Size: 78 6085
XmaI C'CCGG_G
Cuts at: 1870 2859 3488 1870
Size: 989 629 4545
Fragments arranged by size:
4545 989 629
XmnI GAAnn'nnTTC
Cuts at: 3156 4292 3156
Size: 1136 5027
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3971 276
Size: 53 83 186 936 2437 2468
Fragments arranged by size:
2468 2437 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI
BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI
PacI PmlI PsrI RsrII SacII SexAI SfiI SrfI
SwaI XcmI