Restriction map for
pFN21AE1939
(Circular) (Six-base) MAPSORT of: pFN21AE1939.seq Check: 9317 from: 1 to: 6148
pFN21AE1939 6148 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2337 2337
Size: 6148
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3958 278
Size: 53 83 186 936 2422 2468
Fragments arranged by size:
2468 2422 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2835 3491 1937
Size: 898 656 4594
Fragments arranged by size:
4594 898 656
Acc65I G'GTAC_C
Cuts at: 3469 3469
Size: 6148
AclI AA'CG_TT
Cuts at: 1563 4275 4648 1563
Size: 2712 373 3063
Fragments arranged by size:
3063 2712 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3040 3230 3383 4211 5595 5863 1632
Size: 1408 190 153 828 1384 268 1917
Fragments arranged by size:
1917 1408 1384 828 268 190 153
AfeI AGC'GCT
Cuts at: 3282 3282
Size: 6148
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5951
AflIII A'CryG_T
Cuts at: 1203 2511 5047 6024 1203
Size: 1308 2536 977 1327
Fragments arranged by size:
2536 1327 1308 977
AgeI A'CCGG_T
Cuts at: 4979 4979
Size: 6148
AhdI GACnn_n'nnGTC
Cuts at: 2975 4877 2975
Size: 1902 4246
AleI CACnn'nnGTG
Cuts at: 1166 2259 3161 1166
Size: 1093 902 4153
Fragments arranged by size:
4153 1093 902
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6148
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3398 3430 3398
Size: 32 6116
AlwNI CAG_nnn'CTG
Cuts at: 2305 2468 2731 3891 5463 2305
Size: 163 263 1160 1572 2990
Fragments arranged by size:
2990 1572 1160 263 163
ApaLI G'TGCA_C
Cuts at: 4205 5361 4205
Size: 1156 4992
ApoI r'AATT_y
Cuts at: 1493 3457 3683 3835 5944 1493
Size: 1964 226 152 2109 1697
Fragments arranged by size:
2109 1964 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4702 160
Size: 872 3670 1606
Fragments arranged by size:
3670 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6148
AvaI C'yCGr_G
Cuts at: 1870 1943 2712 3473 1870
Size: 73 769 761 4545
Fragments arranged by size:
4545 769 761 73
BamHI G'GATC_C
Cuts at: 2205 3478 3845 2205
Size: 1273 367 4508
Fragments arranged by size:
4508 1273 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2078 3422 3469 4924 618
Size: 325 217 627 291 1344 47 1455 1842
Fragments arranged by size:
1842 1455 1344 627 325 291 217 47
BanII G_rGCy'C
Cuts at: 1391 2026 2387 3467 1391
Size: 635 361 1080 4072
Fragments arranged by size:
4072 1080 635 361
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2851 3130 928
Size: 971 952 279 3946
Fragments arranged by size:
3946 971 952 279
BbvCI CC'TCA_GC
Cuts at: 2282 2282
Size: 6148
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2473 2507 4339 4373 1903
Size: 34 536 34 1832 34 3678
Fragments arranged by size:
3678 1832 536 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4040 5250 4040
Size: 1210 4938
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6148
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2714 4759
Size: 107 122 71 631 605 337 705 2045
Cuts at: 4759 136
Size: 1525
Fragments arranged by size:
2045 1525 705 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 6143 6143
Size: 6148
BlpI GC'TnA_GC
Cuts at: 3565 3565
Size: 6148
Bme1580I G_kGCm'C
Cuts at: 1790 3036 4209 5365 1790
Size: 1246 1173 1156 2573
Fragments arranged by size:
2573 1246 1173 1156
BmgBI CAC'GTC
Cuts at: 1318 2215 2500 1318
Size: 897 285 4966
Fragments arranged by size:
4966 897 285
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3210 3879 4837
Size: 416 53 83 399 24 1793 669 958
Cuts at: 4837 442
Size: 1753
Fragments arranged by size:
1793 1753 958 669 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2458 2603 2753 2763 3005 3106 4808 1239
Size: 1219 145 150 10 242 101 1702 2579
Fragments arranged by size:
2579 1702 1219 242 150 145 101 10
Bpu10I CC'TnA_GC
Cuts at: 2168 2282 2168
Size: 114 6034
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2221 4273 5138 5436 2221
Size: 2052 865 298 2933
Fragments arranged by size:
2933 2052 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4811 882
Size: 617 3312 2219
Fragments arranged by size:
3312 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3939 493
Size: 711 2735 2702
Fragments arranged by size:
2735 2702 711
BsaBI GATnn'nnATC
Cuts at: 6142 6142
Size: 6148
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3955 4337 4972 275
Size: 53 83 186 936 2422 382 635 1451
Fragments arranged by size:
2422 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4581 4979 5253 5400 5811 4581
Size: 398 274 147 411 4918
Fragments arranged by size:
4918 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2726 2756 1178
Size: 30 165 30 1323 30 4570
Fragments arranged by size:
4570 1323 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2317 3275 1186
Size: 1131 958 4059
Fragments arranged by size:
4059 1131 958
BseYI C'CCAG_C
Cuts at: 3116 5351 5847 3116
Size: 2235 496 3417
Fragments arranged by size:
3417 2235 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5575
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2221 3598 4359 5387 1540
Size: 275 186 220 1377 761 1028 2301
Fragments arranged by size:
2301 1377 1028 761 275 220 186
BsiHKAI G_wGCw'C
Cuts at: 3111 4209 4294 5365 3111
Size: 1098 85 1071 3894
Fragments arranged by size:
3894 1098 1071 85
BsmI GAATG_Cn'
Cuts at: 3659 3752 3659
Size: 93 6055
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2026 2387 3036 3111 3467 4209 4294
Size: 399 236 361 649 75 356 742 85
Cuts at: 4294 5365 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 649 399 361 356 236
85 75
BspEI T'CCGG_A
Cuts at: 5811 5811
Size: 6148
BspHI T'CATG_A
Cuts at: 2802 2884 4037 5767 2802
Size: 82 1153 1730 3183
Fragments arranged by size:
3183 1730 1153 82
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2337 3503 844
Size: 1493 1166 3489
Fragments arranged by size:
3489 1493 1166
BsrBI CCG'CTC
Cuts at: 1394 1823 3046 4035 1394
Size: 429 1223 989 3507
Fragments arranged by size:
3507 1223 989 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2045 4643 4817 65
Size: 1183 797 2598 174 1396
Fragments arranged by size:
2598 1396 1183 797 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4792 4979 1700
Size: 110 2982 187 2869
Fragments arranged by size:
2982 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6148
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6148
BssSI C'ACGA_G
Cuts at: 4208 5220 4208
Size: 1012 5136
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2595 794
Size: 1801 4347
BstBI TT'CG_AA
Cuts at: 4952 4952
Size: 6148
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6148
BstXI CCAn_nnnn'nTGG
Cuts at: 2041 2041
Size: 6148
BstYI r'GATC_y
Cuts at: 1972 2205 3396 3478 3845 4230 4247 5699 5785
Size: 233 1191 82 367 385 17 1452 86
Cuts at: 5785 5797 6143 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1191 385 367 346 233 86
82 17 12
Bsu36I CC'TnA_GG
Cuts at: 2814 2814
Size: 6148
BtgI C'CryG_G
Cuts at: 513 1065 2004 2221 2981 513
Size: 552 939 217 760 3680
Fragments arranged by size:
3680 939 760 552 217
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2476 487
Size: 683 56 1250 4159
Fragments arranged by size:
4159 1250 683 56
BtsI GCAGTG_nn'
Cuts at: 2491 3664 4458 4478 2491
Size: 1173 794 20 4161
Fragments arranged by size:
4161 1173 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6148
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6113
DraI TTT'AAA
Cuts at: 3452 3452
Size: 6148
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2959 5155 809
Size: 507 1643 2196 1802
Fragments arranged by size:
2196 1802 1643 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2016 2218 2296
Size: 54 1425 50 275 18 186 202 78
Cuts at: 2296 2868 3153 3336 3595 4484 6113 8
Size: 572 285 183 259 889 1629 43
Fragments arranged by size:
1629 1425 889 572 285 275 259 202
186 183 78 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2218 3595 1537
Size: 275 406 1377 4090
Fragments arranged by size:
4090 1377 406 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2549 4078 1353
Size: 1196 1529 3423
Fragments arranged by size:
3423 1529 1196
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3157 4735 5109 5255 387
Size: 1064 138 1568 1578 374 146 1280
Fragments arranged by size:
1578 1568 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2458 2603 2753 2763 3005 3040 3106
Size: 393 826 145 150 10 242 35 66
Cuts at: 3106 3230 3383 4211 4808 5595 5863 1239
Size: 124 153 828 597 787 268 1524
Fragments arranged by size:
1524 828 826 787 597 393 268 242
153 150 145 124 66 35 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2452 2813 2452
Size: 361 5787
EcoO109I rG'GnC_Cy
Cuts at: 2010 2302 2010
Size: 292 5856
EcoRI G'AATT_C
Cuts at: 3457 3835 3457
Size: 378 5770
EcoRV GAT'ATC
Cuts at: 2897 2897
Size: 6148
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2386 2386
Size: 6148
FspI TGC'GCA
Cuts at: 2360 4654 2360
Size: 2294 3854
HaeII r_GCGC'y
Cuts at: 1678 2230 3284 5295 1678
Size: 552 1054 2011 2531
Fragments arranged by size:
2531 2011 1054 552
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2961 2993 4789 4821 4863
Size: 32 135 32 1389 32 1796 32 42
Cuts at: 4863 4895 1373
Size: 32 2626
Fragments arranged by size:
2626 1796 1389 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2425 3492 3738 669
Size: 988 281 487 1067 246 3079
Fragments arranged by size:
3079 1067 988 487 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6148
HpaI GTT'AAC
Cuts at: 3738 3738
Size: 6148
KpnI G_GTAC'C
Cuts at: 3473 3473
Size: 6148
MfeI C'AATT_G
Cuts at: 3747 3747
Size: 6148
MluI A'CGCG_T
Cuts at: 6024 6024
Size: 6148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2346 2971 2992 3232 3255 5262 5446 1305
Size: 1041 625 21 240 23 2007 184 2007
Fragments arranged by size:
2007 2007 1041 625 240 184 23 21
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2018 2298 3155 10
Size: 54 1425 343 186 280 857 3003
Fragments arranged by size:
3003 1425 857 343 280 186 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2259 3161 4106 4465 4624 518
Size: 648 1093 902 945 359 159 2042
Fragments arranged by size:
2042 1093 945 902 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2259 2478 3564 4241 5389 5634 1606
Size: 653 219 1086 677 1148 245 2120
Fragments arranged by size:
2120 1148 1086 677 653 245 219
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4657
Fragments arranged by size:
4657 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6148
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6148
NotI GC'GGCC_GC
Cuts at: 3595 3595
Size: 6148
NspI r_CATG'y
Cuts at: 1132 1229 2515 3506 5051 1132
Size: 97 1286 991 1545 2229
Fragments arranged by size:
2229 1545 1286 991 97
PasI CC'CwG_GG
Cuts at: 1348 3062 1348
Size: 1714 4434
PciI A'CATG_T
Cuts at: 2511 5047 2511
Size: 2536 3612
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2731 1108
Size: 1623 4525
PfoI T'CCnGG_A
Cuts at: 2450 2450
Size: 6148
PmeI GTTT'AAAC
Cuts at: 3452 3452
Size: 6148
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2552 2584 4217 4249 5730 5762 2552
Size: 32 1633 32 1481 32 2938
Fragments arranged by size:
2938 1633 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6148
PsiI TTA'TAA
Cuts at: 3718 3718
Size: 6148
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2758 2790 2758
Size: 32 6116
PstI C_TGCA'G
Cuts at: 830 2124 2157 2483 2800 3396 3500 830
Size: 1294 33 326 317 596 104 3478
Fragments arranged by size:
3478 1294 596 326 317 104 33
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6148
PvuII CAG'CTG
Cuts at: 2259 2478 2259
Size: 219 5929
SalI G'TCGA_C
Cuts at: 1936 3490 1936
Size: 1554 4594
SbfI CC_TGCA'GG
Cuts at: 3500 3500
Size: 6148
ScaI AGT'ACT
Cuts at: 2580 4396 6108 2580
Size: 1816 1712 2620
Fragments arranged by size:
2620 1816 1712
SfcI C'TryA_G
Cuts at: 826 1045 2120 2153 2378 2479 2630 2796 3392
Size: 219 1075 33 225 101 151 166 596
Cuts at: 3392 3496 4631 5312 5503 826
Size: 104 1135 681 191 1471
Fragments arranged by size:
1471 1135 1075 681 596 225 219 191
166 151 104 101 33
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6148
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6148
SmaI CCC'GGG
Cuts at: 1872 3475 1872
Size: 1603 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2200 4252 5153 5415 820
Size: 197 926 257 2052 901 262 1553
Fragments arranged by size:
2052 1553 926 901 262 257 197
SnaBI TAC'GTA
Cuts at: 493 3939 493
Size: 3446 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6148
SphI G_CATG'C
Cuts at: 1132 3506 1132
Size: 2374 3774
SspI AAT'ATT
Cuts at: 5 52 4072 5
Size: 47 4020 2081
Fragments arranged by size:
4020 2081 47
StuI AGG'CCT
Cuts at: 1517 2287 1517
Size: 770 5378
StyI C'CwwG_G
Cuts at: 513 1065 2004 2013 3385 3587 513
Size: 552 939 9 1372 202 3074
Fragments arranged by size:
3074 1372 939 552 202 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2066 2410 3410 4175 4192 4345 4530
Size: 1255 486 344 1000 765 17 153 185
Cuts at: 4530 4975 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1000 765 486 445 344 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2578 4394 6106
Size: 274 80 33 51 1443 601 1816 1712
Cuts at: 6106 96
Size: 138
Fragments arranged by size:
1816 1712 1443 601 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6108 6140 6108
Size: 32 6116
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6148
XbaI T'CTAG_A
Cuts at: 3484 3484
Size: 6148
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2089 2219 3010 2011
Size: 78 130 791 5149
Fragments arranged by size:
5149 791 130 78
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6148
XmaI C'CCGG_G
Cuts at: 1870 3473 1870
Size: 1603 4545
XmnI GAAnn'nnTTC
Cuts at: 2543 2757 4277 2543
Size: 214 1520 4414
Fragments arranged by size:
4414 1520 214
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3956 276
Size: 53 83 186 936 2422 2468
Fragments arranged by size:
2468 2422 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoICRI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI