Restriction map for
pFN21AE1935
(Circular) (Six-base) MAPSORT of: pFN21AE1935.seq Check: 9303 from: 1 to: 6127
pFN21AE1935 6127 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3937 278
Size: 53 83 186 936 2401 2468
Fragments arranged by size:
2468 2401 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3041 3470 1937
Size: 1104 429 4594
Fragments arranged by size:
4594 1104 429
Acc65I G'GTAC_C
Cuts at: 3448 3448
Size: 6127
AclI AA'CG_TT
Cuts at: 1563 4254 4627 1563
Size: 2691 373 3063
Fragments arranged by size:
3063 2691 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2790 2835 4190 5574 5842 1632
Size: 1158 45 1355 1384 268 1917
Fragments arranged by size:
1917 1384 1355 1158 268 45
AfeI AGC'GCT
Cuts at: 3338 3338
Size: 6127
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5930
AflIII A'CryG_T
Cuts at: 1203 5026 6003 1203
Size: 3823 977 1327
Fragments arranged by size:
3823 1327 977
AgeI A'CCGG_T
Cuts at: 4958 4958
Size: 6127
AhdI GACnn_n'nnGTC
Cuts at: 4856 4856
Size: 6127
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6127
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6127
AlwNI CAG_nnn'CTG
Cuts at: 2327 3870 5442 2327
Size: 1543 1572 3012
Fragments arranged by size:
3012 1572 1543
ApaI G_GGCC'C
Cuts at: 3173 3173
Size: 6127
ApaLI G'TGCA_C
Cuts at: 2935 4184 5340 2935
Size: 1249 1156 3722
Fragments arranged by size:
3722 1249 1156
ApoI r'AATT_y
Cuts at: 1493 2096 2156 2399 2881 3436 3662 3814 5923
Size: 603 60 243 482 555 226 152 2109
Cuts at: 5923 1493
Size: 1697
Fragments arranged by size:
2109 1697 603 555 482 243 226 152
60
AseI AT'TA_AT
Cuts at: 160 1032 4681 160
Size: 872 3649 1606
Fragments arranged by size:
3649 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
AvaI C'yCGr_G
Cuts at: 1870 1943 2966 3452 1870
Size: 73 1023 486 4545
Fragments arranged by size:
4545 1023 486 73
BamHI G'GATC_C
Cuts at: 3457 3824 3457
Size: 367 5760
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3448 4903 618
Size: 325 217 627 1661 1455 1842
Fragments arranged by size:
1842 1661 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3173 3446 1391
Size: 1782 273 4072
Fragments arranged by size:
4072 1782 273
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2901 3147 928
Size: 971 1002 246 3908
Fragments arranged by size:
3908 1002 971 246
BbvCI CC'TCA_GC
Cuts at: 2269 2269
Size: 6127
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2349 2383 4318 4352 1903
Size: 34 412 34 1935 34 3678
Fragments arranged by size:
3678 1935 412 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4019 5229 4019
Size: 1210 4917
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6127
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4738 136
Size: 107 122 71 631 605 3066 1525
Fragments arranged by size:
3066 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6122 6122
Size: 6127
BlpI GC'TnA_GC
Cuts at: 2702 3544 2702
Size: 842 5285
Bme1580I G_kGCm'C
Cuts at: 1790 2939 3173 4188 5344 1790
Size: 1149 234 1015 1156 2573
Fragments arranged by size:
2573 1156 1149 1015 234
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6127
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2157 3858 4816
Size: 416 53 83 399 24 740 1701 958
Cuts at: 4816 442
Size: 1753
Fragments arranged by size:
1753 1701 958 740 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6127
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2827 2827
Size: 6127
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2600 2768 4787 1239
Size: 1361 168 2019 2579
Fragments arranged by size:
2579 2019 1361 168
Bpu10I CC'TnA_GC
Cuts at: 2269 3165 2269
Size: 896 5231
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3119 4252 5117 5415 3119
Size: 1133 865 298 3831
Fragments arranged by size:
3831 1133 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4790 882
Size: 617 3291 2219
Fragments arranged by size:
3291 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2413 3918 493
Size: 711 1209 1505 2702
Fragments arranged by size:
2702 1505 1209 711
BsaBI GATnn'nnATC
Cuts at: 6121 6121
Size: 6127
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3934 4316 4951 275
Size: 53 83 186 936 2401 382 635 1451
Fragments arranged by size:
2401 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3073 4560 4958 5232 5379 5790 3073
Size: 1487 398 274 147 411 3410
Fragments arranged by size:
3410 1487 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2774 2804 3335 3365 1178
Size: 30 165 30 1371 30 531 30 3940
Fragments arranged by size:
3940 1371 531 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2505 3369 1186
Size: 1319 864 3944
Fragments arranged by size:
3944 1319 864
BseYI C'CCAG_C
Cuts at: 5330 5826 5330
Size: 496 5631
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2323 3246 1163
Size: 573 587 923 4044
Fragments arranged by size:
4044 923 587 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3577 4338 5366 1540
Size: 275 186 1576 761 1028 2301
Fragments arranged by size:
2301 1576 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2939 4188 4273 5344 2939
Size: 1249 85 1071 3722
Fragments arranged by size:
3722 1249 1071 85
BsmI GAATG_Cn'
Cuts at: 3131 3638 3731 3131
Size: 507 93 5527
Fragments arranged by size:
5527 507 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2939 3173 3446 4188 4273 5344 1391
Size: 399 1149 234 273 742 85 1071 2174
Fragments arranged by size:
2174 1149 1071 742 399 273 234 85
BspEI T'CCGG_A
Cuts at: 3073 5790 3073
Size: 2717 3410
BspHI T'CATG_A
Cuts at: 4016 5746 4016
Size: 1730 4397
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3482 844
Size: 2638 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4014 1394
Size: 429 2191 3507
Fragments arranged by size:
3507 2191 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4622 4796 65
Size: 1183 3374 174 1396
Fragments arranged by size:
3374 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4771 4958 1700
Size: 110 2961 187 2869
Fragments arranged by size:
2961 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2145 96
Size: 2049 4078
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6127
BssSI C'ACGA_G
Cuts at: 4187 5199 4187
Size: 1012 5115
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2279 794
Size: 1485 4642
BstBI TT'CG_AA
Cuts at: 4931 4931
Size: 6127
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6127
BstXI CCAn_nnnn'nTGG
Cuts at: 3031 3316 3031
Size: 285 5842
BstYI r'GATC_y
Cuts at: 1972 2455 3457 3824 4209 4226 5678 5764 5776
Size: 483 1002 367 385 17 1452 86 12
Cuts at: 5776 6122 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1002 483 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 3042 3042
Size: 6127
Bsu36I CC'TnA_GG
Cuts at: 3297 3297
Size: 6127
BtgI C'CryG_G
Cuts at: 513 1065 2919 3309 513
Size: 552 1854 390 3331
Fragments arranged by size:
3331 1854 552 390
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5388
Fragments arranged by size:
5388 683 56
BtsI GCAGTG_nn'
Cuts at: 2325 3643 4437 4457 2325
Size: 1318 794 20 3995
Fragments arranged by size:
3995 1318 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6127
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2465 2500 529
Size: 35 1901 35 4156
Fragments arranged by size:
4156 1901 35 35
DraI TTT'AAA
Cuts at: 3431 3431
Size: 6127
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5134 809
Size: 507 3818 1802
Fragments arranged by size:
3818 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2826 3574 4463
Size: 54 1425 50 275 18 996 748 889
Cuts at: 4463 6092 8
Size: 1629 43
Fragments arranged by size:
1629 1425 996 889 748 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3574 1537
Size: 275 1762 4090
Fragments arranged by size:
4090 1762 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2003 4057 1353
Size: 650 2054 3423
Fragments arranged by size:
3423 2054 650
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4714 5088 5234 387
Size: 1064 138 3125 374 146 1280
Fragments arranged by size:
3125 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2600 2768 2790 2835 4190 4787 5574
Size: 393 968 168 22 45 1355 597 787
Cuts at: 5574 5842 1239
Size: 268 1524
Fragments arranged by size:
1524 1355 968 787 597 393 268 168
45 22
EcoNI CCTnn'n_nnAGG
Cuts at: 3089 3089
Size: 6127
EcoO109I rG'GnC_Cy
Cuts at: 2014 2265 2350 2565 3169 3170 2014
Size: 251 85 215 604 1 4971
Fragments arranged by size:
4971 604 251 215 85 1
EcoRI G'AATT_C
Cuts at: 2096 2399 2881 3436 3814 2096
Size: 303 482 555 378 4409
Fragments arranged by size:
4409 555 482 378 303
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2137 2137
Size: 6127
FspI TGC'GCA
Cuts at: 4633 4633
Size: 6127
HaeII r_GCGC'y
Cuts at: 1678 3340 5274 1678
Size: 1662 1934 2531
Fragments arranged by size:
2531 1934 1662
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2111 2143 2678 2710 2855
Size: 32 135 32 539 32 535 32 145
Cuts at: 2855 2887 3335 3367 4768 4800 4842 4874 1373
Size: 32 448 32 1401 32 42 32 2626
Fragments arranged by size:
2626 1401 539 535 448 145 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3331 3471 3717 669
Size: 988 281 1393 140 246 3079
Fragments arranged by size:
3079 1393 988 281 246 140
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6127
HpaI GTT'AAC
Cuts at: 3331 3717 3331
Size: 386 5741
KpnI G_GTAC'C
Cuts at: 3452 3452
Size: 6127
MfeI C'AATT_G
Cuts at: 3726 3726
Size: 6127
MluI A'CGCG_T
Cuts at: 6003 6003
Size: 6127
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3378 5241 5425 1305
Size: 2073 1863 184 2007
Fragments arranged by size:
2073 2007 1863 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2828 10
Size: 54 1425 343 996 3309
Fragments arranged by size:
3309 1425 996 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4085 4444 4603 518
Size: 648 2919 359 159 2042
Fragments arranged by size:
2919 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2954 3543 4220 5368 5613 1606
Size: 1348 589 677 1148 245 2120
Fragments arranged by size:
2120 1348 1148 677 589 245
NcoI C'CATG_G
Cuts at: 513 1065 2919 513
Size: 552 1854 3721
Fragments arranged by size:
3721 1854 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6127
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6127
NotI GC'GGCC_GC
Cuts at: 3574 3574
Size: 6127
NspI r_CATG'y
Cuts at: 1132 1229 2051 3485 5030 1132
Size: 97 822 1434 1545 2229
Fragments arranged by size:
2229 1545 1434 822 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6127
PciI A'CATG_T
Cuts at: 5026 5026
Size: 6127
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6127
PfoI T'CCnGG_A
Cuts at: 3013 3013
Size: 6127
PmeI GTTT'AAAC
Cuts at: 3431 3431
Size: 6127
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2349 2381 4196 4228 5709 5741 2349
Size: 32 1815 32 1481 32 2735
Fragments arranged by size:
2735 1815 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2014 2265 2565 2014
Size: 251 300 5576
Fragments arranged by size:
5576 300 251
PshAI GACnn'nnGTC
Cuts at: 1613 2901 1613
Size: 1288 4839
PsiI TTA'TAA
Cuts at: 3697 3697
Size: 6127
PspOMI G'GGCC_C
Cuts at: 3169 3169
Size: 6127
PstI C_TGCA'G
Cuts at: 830 3479 830
Size: 2649 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6127
PvuII CAG'CTG
Cuts at: 2954 2954
Size: 6127
SalI G'TCGA_C
Cuts at: 1936 3469 1936
Size: 1533 4594
SbfI CC_TGCA'GG
Cuts at: 3479 3479
Size: 6127
ScaI AGT'ACT
Cuts at: 4375 6087 4375
Size: 1712 4415
SfcI C'TryA_G
Cuts at: 826 1045 3475 4610 5291 5482 826
Size: 219 2430 1135 681 191 1471
Fragments arranged by size:
2430 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6127
SmaI CCC'GGG
Cuts at: 1872 3454 1872
Size: 1582 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3098 4231 5132 5394 820
Size: 197 926 1155 1133 901 262 1553
Fragments arranged by size:
1553 1155 1133 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3918 493
Size: 3425 2702
SpeI A'CTAG_T
Cuts at: 152 2137 152
Size: 1985 4142
SphI G_CATG'C
Cuts at: 1132 3485 1132
Size: 2353 3774
SspI AAT'ATT
Cuts at: 5 52 2492 4051 5
Size: 47 2440 1559 2081
Fragments arranged by size:
2440 2081 1559 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6127
StyI C'CwwG_G
Cuts at: 513 1065 2919 3118 3566 513
Size: 552 1854 199 448 3074
Fragments arranged by size:
3074 1854 552 448 199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2231 2334 3322 4154 4171 4324 4509
Size: 1255 651 103 988 832 17 153 185
Cuts at: 4509 4954 325
Size: 445 1498
Fragments arranged by size:
1498 1255 988 832 651 445 185 153
103 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2145 3255 4373
Size: 274 80 33 51 1443 168 1110 1118
Cuts at: 4373 6085 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1118 1110 274 168 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2385 2417 6087 6119 2385
Size: 32 3670 32 2393
Fragments arranged by size:
3670 2393 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6127
XbaI T'CTAG_A
Cuts at: 3463 3463
Size: 6127
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2350 2350
Size: 6127
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6127
XmaI C'CCGG_G
Cuts at: 1870 3452 1870
Size: 1582 4545
XmnI GAAnn'nnTTC
Cuts at: 4256 4256
Size: 6127
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3935 276
Size: 53 83 186 936 2401 2468
Fragments arranged by size:
2468 2401 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI DraIII EcoICRI EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI