Restriction map for
pFN21AE1927
(Circular) (Six-base) MAPSORT of: pFN21AE1927.seq Check: 6019 from: 1 to: 6004
pFN21AE1927 6004 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2065 2065
Size: 6004
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3301 3814 278
Size: 53 83 186 936 1765 513 2468
Fragments arranged by size:
2468 1765 936 513 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3347 1937
Size: 1410 4594
Acc65I G'GTAC_C
Cuts at: 3325 3325
Size: 6004
AclI AA'CG_TT
Cuts at: 1563 4131 4504 1563
Size: 2568 373 3063
Fragments arranged by size:
3063 2568 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2828 3130 4067 5451 5719 1632
Size: 1196 302 937 1384 268 1917
Fragments arranged by size:
1917 1384 1196 937 302 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5807
AflIII A'CryG_T
Cuts at: 1203 2104 3105 4903 5880 1203
Size: 901 1001 1798 977 1327
Fragments arranged by size:
1798 1327 1001 977 901
AgeI A'CCGG_T
Cuts at: 4835 4835
Size: 6004
AhdI GACnn_n'nnGTC
Cuts at: 2108 4733 2108
Size: 2625 3379
AleI CACnn'nnGTG
Cuts at: 1166 2882 1166
Size: 1716 4288
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6004
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2243 2275 2243
Size: 32 5972
AlwNI CAG_nnn'CTG
Cuts at: 2124 2164 2512 3747 5319 2124
Size: 40 348 1235 1572 2809
Fragments arranged by size:
2809 1572 1235 348 40
ApaLI G'TGCA_C
Cuts at: 2336 4061 5217 2336
Size: 1725 1156 3123
Fragments arranged by size:
3123 1725 1156
ApoI r'AATT_y
Cuts at: 1493 3049 3313 3539 3691 5800 1493
Size: 1556 264 226 152 2109 1697
Fragments arranged by size:
2109 1697 1556 264 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4558 160
Size: 872 3526 1606
Fragments arranged by size:
3526 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6004
AvaI C'yCGr_G
Cuts at: 1870 1943 2870 2991 3329 1870
Size: 73 927 121 338 4545
Fragments arranged by size:
4545 927 338 121 73
BamHI G'GATC_C
Cuts at: 2017 3334 3701 2017
Size: 1317 367 4320
Fragments arranged by size:
4320 1317 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3160 3181 3325 4780 618
Size: 325 217 627 1373 21 144 1455 1842
Fragments arranged by size:
1842 1455 1373 627 325 217 144 21
BanII G_rGCy'C
Cuts at: 1391 2439 3323 1391
Size: 1048 884 4072
Fragments arranged by size:
4072 1048 884
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2107 2816 3031 3301 928
Size: 971 208 709 215 270 3631
Fragments arranged by size:
3631 971 709 270 215 208
BbvCI CC'TCA_GC
Cuts at: 2642 2642
Size: 6004
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4195 4229 1903
Size: 34 2258 34 3678
Fragments arranged by size:
3678 2258 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3896 5106 3896
Size: 1210 4794
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6004
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4615 136
Size: 107 122 71 631 605 2943 1525
Fragments arranged by size:
2943 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2919 3141 5999 2919
Size: 222 2858 2924
Fragments arranged by size:
2924 2858 222
BlpI GC'TnA_GC
Cuts at: 2027 3421 2027
Size: 1394 4610
Bme1580I G_kGCm'C
Cuts at: 1790 2340 3091 3099 4065 5221 1790
Size: 550 751 8 966 1156 2573
Fragments arranged by size:
2573 1156 966 751 550 8
BmgBI CAC'GTC
Cuts at: 1318 2053 2105 1318
Size: 735 52 5217
Fragments arranged by size:
5217 735 52
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3735 4693 442
Size: 416 53 83 399 24 2318 958 1753
Fragments arranged by size:
2318 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6004
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2189 2258 2489 3176 4664 1239
Size: 950 69 231 687 1488 2579
Fragments arranged by size:
2579 1488 950 687 231 69
Bpu10I CC'TnA_GC
Cuts at: 2642 2642
Size: 6004
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4129 4994 5292 4129
Size: 865 298 4841
Fragments arranged by size:
4841 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3079 4667 882
Size: 617 1580 1588 2219
Fragments arranged by size:
2219 1588 1580 617
BsaAI yAC'GTr
Cuts at: 493 1204 2332 3795 493
Size: 711 1128 1463 2702
Fragments arranged by size:
2702 1463 1128 711
BsaBI GATnn'nnATC
Cuts at: 5998 5998
Size: 6004
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2528 2586 3298 3811
Size: 53 83 186 936 995 58 712 513
Cuts at: 3811 4193 4828 275
Size: 382 635 1451
Fragments arranged by size:
1451 995 936 712 635 513 382 186
83 58 53
BsaWI w'CCGG_w
Cuts at: 2697 4437 4835 5109 5256 5667 2697
Size: 1740 398 274 147 411 3034
Fragments arranged by size:
3034 1740 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2518 2548 2916 2946 3253
Size: 30 165 30 1115 30 368 30 307
Cuts at: 3253 3283 1178
Size: 30 3899
Fragments arranged by size:
3899 1115 368 307 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3067 1186
Size: 1881 4123
BseYI C'CCAG_C
Cuts at: 2832 5207 5703 2832
Size: 2375 496 3133
Fragments arranged by size:
3133 2375 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2498 2735 1163
Size: 573 762 237 4432
Fragments arranged by size:
4432 762 573 237
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3454 4215 5243 1540
Size: 275 186 1453 761 1028 2301
Fragments arranged by size:
2301 1453 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2033 2340 2439 2943 3174 4065 4150 5221 2033
Size: 307 99 504 231 891 85 1071 2816
Fragments arranged by size:
2816 1071 891 504 307 231 99 85
BsmI GAATG_Cn'
Cuts at: 2067 3515 3608 2067
Size: 1448 93 4463
Fragments arranged by size:
4463 1448 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3305 3305
Size: 6004
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2033 2340 2439 2943 3091 3099 3174
Size: 399 243 307 99 504 148 8 75
Cuts at: 3174 3323 4065 4150 5221 1391
Size: 149 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 504 399 307 243 149
148 99 85 75 8
BspEI T'CCGG_A
Cuts at: 2697 5667 2697
Size: 2970 3034
BspHI T'CATG_A
Cuts at: 3893 5623 3893
Size: 1730 4274
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2065 3359 844
Size: 1221 1294 3489
Fragments arranged by size:
3489 1294 1221
BsrBI CCG'CTC
Cuts at: 1394 1823 3891 1394
Size: 429 2068 3507
Fragments arranged by size:
3507 2068 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4499 4673 65
Size: 1183 3251 174 1396
Fragments arranged by size:
3251 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4648 4835 1700
Size: 110 2838 187 2869
Fragments arranged by size:
2869 2838 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6004
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6004
BssSI C'ACGA_G
Cuts at: 2032 2144 2516 4064 5076 2032
Size: 112 372 1548 1012 2960
Fragments arranged by size:
2960 1548 1012 372 112
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6004
BstBI TT'CG_AA
Cuts at: 4808 4808
Size: 6004
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6004
BstYI r'GATC_y
Cuts at: 1972 2017 2702 2730 2919 3141 3334 3701 4086
Size: 45 685 28 189 222 193 367 385
Cuts at: 4086 4103 5555 5641 5653 5999 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 685 385 367 346 222 193
189 86 45 28 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2555 3185 3230 3243 513
Size: 552 939 551 630 45 13 3274
Fragments arranged by size:
3274 939 630 552 551 45 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5265
Fragments arranged by size:
5265 683 56
BtsI GCAGTG_nn'
Cuts at: 3520 4314 4334 3520
Size: 794 20 5190
Fragments arranged by size:
5190 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6004
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5969
DraI TTT'AAA
Cuts at: 3308 3308
Size: 6004
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2465 5011 809
Size: 507 1149 2546 1802
Fragments arranged by size:
2546 1802 1149 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2011 2055 3059
Size: 54 1425 50 275 18 181 44 1004
Cuts at: 3059 3451 4340 5969 8
Size: 392 889 1629 43
Fragments arranged by size:
1629 1425 1004 889 392 275 181 54
50 44 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3451 1537
Size: 275 1639 4090
Fragments arranged by size:
4090 1639 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3934 1353
Size: 2581 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2695 4591 4965 5111 387
Size: 1064 138 1106 1896 374 146 1280
Fragments arranged by size:
1896 1280 1106 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2437 2437
Size: 6004
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2189 2258 2489 2828 3130 3176 4067
Size: 393 557 69 231 339 302 46 891
Cuts at: 4067 4664 5451 5719 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 891 787 597 557 393 339 302
268 231 69 46
EcoNI CCTnn'n_nnAGG
Cuts at: 2516 2516
Size: 6004
EcoO109I rG'GnC_Cy
Cuts at: 2323 2359 2689 2323
Size: 36 330 5638
Fragments arranged by size:
5638 330 36
EcoRI G'AATT_C
Cuts at: 3313 3691 3313
Size: 378 5626
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2332 2332
Size: 6004
FspI TGC'GCA
Cuts at: 2681 4510 2681
Size: 1829 4175
HaeII r_GCGC'y
Cuts at: 1678 5151 1678
Size: 3473 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2020 2052 2094 2126 2518
Size: 32 135 32 448 32 42 32 392
Cuts at: 2518 2550 4645 4677 4719 4751 1373
Size: 32 2095 32 42 32 2626
Fragments arranged by size:
2626 2095 448 392 135 42 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3348 3594 669
Size: 988 281 1410 246 3079
Fragments arranged by size:
3079 1410 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6004
HpaI GTT'AAC
Cuts at: 3594 3594
Size: 6004
KpnI G_GTAC'C
Cuts at: 3329 3329
Size: 6004
MfeI C'AATT_G
Cuts at: 3603 3603
Size: 6004
MluI A'CGCG_T
Cuts at: 5880 5880
Size: 6004
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5118 5302 1305
Size: 3813 184 2007
Fragments arranged by size:
3813 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2013 2057 3061 10
Size: 54 1425 343 181 44 1004 2953
Fragments arranged by size:
2953 1425 1004 343 181 54 44
MslI CAynn'nnrTG
Cuts at: 518 1166 2882 3962 4321 4480 518
Size: 648 1716 1080 359 159 2042
Fragments arranged by size:
2042 1716 1080 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2121 2161 2464 2557 2566 3420 4097 5245
Size: 515 40 303 93 9 854 677 1148
Cuts at: 5245 5490 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 854 677 515 303 245 93
40 9
NcoI C'CATG_G
Cuts at: 513 1065 2004 3185 513
Size: 552 939 1181 3332
Fragments arranged by size:
3332 1181 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6004
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6004
NotI GC'GGCC_GC
Cuts at: 3451 3451
Size: 6004
NspI r_CATG'y
Cuts at: 1132 1229 2984 3109 3362 4907 1132
Size: 97 1755 125 253 1545 2229
Fragments arranged by size:
2229 1755 1545 253 125 97
PasI CC'CwG_GG
Cuts at: 1348 2263 3067 1348
Size: 915 804 4285
Fragments arranged by size:
4285 915 804
PciI A'CATG_T
Cuts at: 3105 4903 3105
Size: 1798 4206
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2149 1108
Size: 1041 4963
PmeI GTTT'AAAC
Cuts at: 3308 3308
Size: 6004
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2678 2710 4073 4105 5586 5618 2678
Size: 32 1363 32 1481 32 3064
Fragments arranged by size:
3064 1481 1363 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2359 2359
Size: 6004
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6004
PsiI TTA'TAA
Cuts at: 3574 3574
Size: 6004
PstI C_TGCA'G
Cuts at: 830 2076 3356 830
Size: 1246 1280 3478
Fragments arranged by size:
3478 1280 1246
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6004
PvuII CAG'CTG
Cuts at: 2121 2464 2121
Size: 343 5661
SacI G_AGCT'C
Cuts at: 2439 2439
Size: 6004
SacII CC_GC'GG
Cuts at: 2558 2558
Size: 6004
SalI G'TCGA_C
Cuts at: 1936 3346 1936
Size: 1410 4594
SbfI CC_TGCA'GG
Cuts at: 3356 3356
Size: 6004
ScaI AGT'ACT
Cuts at: 3131 4252 5964 3131
Size: 1121 1712 3171
Fragments arranged by size:
3171 1712 1121
SfcI C'TryA_G
Cuts at: 826 1045 2072 3352 4487 5168 5359 826
Size: 219 1027 1280 1135 681 191 1471
Fragments arranged by size:
1471 1280 1135 1027 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6004
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6004
SmaI CCC'GGG
Cuts at: 1872 2993 3331 1872
Size: 1121 338 4545
Fragments arranged by size:
4545 1121 338
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4108 5009 5271 820
Size: 197 926 2165 901 262 1553
Fragments arranged by size:
2165 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3795 493
Size: 3302 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6004
SphI G_CATG'C
Cuts at: 1132 2984 3362 1132
Size: 1852 378 3774
Fragments arranged by size:
3774 1852 378
SspI AAT'ATT
Cuts at: 5 52 3928 5
Size: 47 3876 2081
Fragments arranged by size:
3876 2081 47
StuI AGG'CCT
Cuts at: 1517 2676 2855 1517
Size: 1159 179 4666
Fragments arranged by size:
4666 1159 179
StyI C'CwwG_G
Cuts at: 513 1065 2004 2749 3185 3443 513
Size: 552 939 745 436 258 3074
Fragments arranged by size:
3074 939 745 552 436 258
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4031 4048 4201 4386 4831 325
Size: 1255 2451 17 153 185 445 1498
Fragments arranged by size:
2451 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2181 2304 3129
Size: 274 80 33 51 1443 204 123 825
Cuts at: 3129 4250 5962 96
Size: 1121 1712 138
Fragments arranged by size:
1712 1443 1121 825 274 204 138 123
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2866 2898 5964 5996 2866
Size: 32 3066 32 2874
Fragments arranged by size:
3066 2874 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6004
XbaI T'CTAG_A
Cuts at: 3340 3340
Size: 6004
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6004
XmaI C'CCGG_G
Cuts at: 1870 2991 3329 1870
Size: 1121 338 4545
Fragments arranged by size:
4545 1121 338
XmnI GAAnn'nnTTC
Cuts at: 2813 3107 4133 2813
Size: 294 1026 4684
Fragments arranged by size:
4684 1026 294
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3299 3812 276
Size: 53 83 186 936 1765 513 2468
Fragments arranged by size:
2468 1765 936 513 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI