Restriction map for
pFN21AE1926
(Circular) (Six-base) MAPSORT of: pFN21AE1926.seq Check: 3805 from: 1 to: 6097
pFN21AE1926 6097 bp
With 182 enzymes: SgfI *
July 6, 2012 17:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3907 278
Size: 53 83 186 936 2371 2468
Fragments arranged by size:
2468 2371 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3440 1937
Size: 1503 4594
Acc65I G'GTAC_C
Cuts at: 3418 3418
Size: 6097
AclI AA'CG_TT
Cuts at: 1563 4224 4597 1563
Size: 2661 373 3063
Fragments arranged by size:
3063 2661 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2913 3070 4160 5544 5812 1632
Size: 1281 157 1090 1384 268 1917
Fragments arranged by size:
1917 1384 1281 1090 268 157
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5900
AflIII A'CryG_T
Cuts at: 1203 4996 5973 1203
Size: 3793 977 1327
Fragments arranged by size:
3793 1327 977
AgeI A'CCGG_T
Cuts at: 4928 4928
Size: 6097
AhdI GACnn_n'nnGTC
Cuts at: 4826 4826
Size: 6097
AleI CACnn'nnGTG
Cuts at: 1166 2903 1166
Size: 1737 4360
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2921 1184
Size: 1737 4360
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2202 2234 2855 2887 2202
Size: 32 621 32 5412
Fragments arranged by size:
5412 621 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2296 2404 2617 3840 5412 2296
Size: 108 213 1223 1572 2981
Fragments arranged by size:
2981 1572 1223 213 108
ApaLI G'TGCA_C
Cuts at: 4154 5310 4154
Size: 1156 4941
ApoI r'AATT_y
Cuts at: 1493 3406 3632 3784 5893 1493
Size: 1913 226 152 2109 1697
Fragments arranged by size:
2109 1913 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4651 160
Size: 872 3619 1606
Fragments arranged by size:
3619 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6097
AvaI C'yCGr_G
Cuts at: 1870 1943 3422 1870
Size: 73 1479 4545
Fragments arranged by size:
4545 1479 73
BamHI G'GATC_C
Cuts at: 3427 3794 3427
Size: 367 5730
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2378 2897 3418 4873 618
Size: 325 217 627 591 519 521 1455 1842
Fragments arranged by size:
1842 1455 627 591 521 519 325 217
BanII G_rGCy'C
Cuts at: 1391 2111 2207 2730 3416 1391
Size: 720 96 523 686 4072
Fragments arranged by size:
4072 720 686 523 96
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5126
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4288 4322 1903
Size: 34 2351 34 3678
Fragments arranged by size:
3678 2351 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3989 5199 3989
Size: 1210 4887
BclI T'GATC_A
Cuts at: 1548 2449 1548
Size: 901 5196
BfrBI ATG'CAT
Cuts at: 3200 3200
Size: 6097
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4708 136
Size: 107 122 71 631 605 3036 1525
Fragments arranged by size:
3036 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6092 6092
Size: 6097
BlpI GC'TnA_GC
Cuts at: 3514 3514
Size: 6097
Bme1580I G_kGCm'C
Cuts at: 1790 4158 5314 1790
Size: 2368 1156 2573
Fragments arranged by size:
2573 2368 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6097
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3828 4786 442
Size: 416 53 83 399 24 2411 958 1753
Fragments arranged by size:
2411 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6097
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2624 3200 2624
Size: 576 5521
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2381 2594 4757 1239
Size: 1142 213 2163 2579
Fragments arranged by size:
2579 2163 1142 213
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2930 3239 4222 5087 5385 2930
Size: 309 983 865 298 3642
Fragments arranged by size:
3642 983 865 309 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2561 4760 882
Size: 617 1062 2199 2219
Fragments arranged by size:
2219 2199 1062 617
BsaAI yAC'GTr
Cuts at: 493 1204 3888 493
Size: 711 2684 2702
Fragments arranged by size:
2702 2684 711
BsaBI GATnn'nnATC
Cuts at: 6091 6091
Size: 6097
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3904 4286 4921 275
Size: 53 83 186 936 2371 382 635 1451
Fragments arranged by size:
2371 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4530 4928 5202 5349 5760 4530
Size: 398 274 147 411 4867
Fragments arranged by size:
4867 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2495 2525 1178
Size: 30 165 30 1092 30 4750
Fragments arranged by size:
4750 1092 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3050 1186
Size: 1864 4233
BseYI C'CCAG_C
Cuts at: 2374 5300 5796 2374
Size: 2926 496 2675
Fragments arranged by size:
2926 2675 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2107 1163
Size: 573 371 5153
Fragments arranged by size:
5153 573 371
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3547 4308 5336 1540
Size: 275 186 1546 761 1028 2301
Fragments arranged by size:
2301 1546 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2730 4158 4243 5314 2730
Size: 1428 85 1071 3513
Fragments arranged by size:
3513 1428 1071 85
BsmI GAATG_Cn'
Cuts at: 3200 3608 3701 3200
Size: 408 93 5596
Fragments arranged by size:
5596 408 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2111 2207 2730 3416 4158 4243 5314
Size: 399 321 96 523 686 742 85 1071
Cuts at: 5314 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 686 523 399 321 96
85
BspEI T'CCGG_A
Cuts at: 5760 5760
Size: 6097
BspHI T'CATG_A
Cuts at: 3986 5716 3986
Size: 1730 4367
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3452 844
Size: 2608 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2172 2651 3984 1394
Size: 429 349 479 1333 3507
Fragments arranged by size:
3507 1333 479 429 349
BsrDI GCAATG_nn'
Cuts at: 65 1248 4592 4766 65
Size: 1183 3344 174 1396
Fragments arranged by size:
3344 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2235 4741 4928 1700
Size: 110 425 2506 187 2869
Fragments arranged by size:
2869 2506 425 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6097
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6097
BssSI C'ACGA_G
Cuts at: 4157 5169 4157
Size: 1012 5085
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6097
BstBI TT'CG_AA
Cuts at: 4901 4901
Size: 6097
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6097
BstYI r'GATC_y
Cuts at: 1972 2217 2454 3427 3794 4179 4196 5648 5734
Size: 245 237 973 367 385 17 1452 86
Cuts at: 5734 5746 6092 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 973 385 367 346 245 237
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2135 2352 2940 513
Size: 552 1070 217 588 3670
Fragments arranged by size:
3670 1070 588 552 217
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5358
Fragments arranged by size:
5358 683 56
BtsI GCAGTG_nn'
Cuts at: 3244 3613 4407 4427 3244
Size: 369 794 20 4914
Fragments arranged by size:
4914 794 369 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6097
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2341 2376 529
Size: 35 1777 35 4250
Fragments arranged by size:
4250 1777 35 35
DraI TTT'AAA
Cuts at: 3401 3401
Size: 6097
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3188 5104 809
Size: 507 1872 1916 1802
Fragments arranged by size:
1916 1872 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2652 2976 3274
Size: 54 1425 50 275 18 822 324 298
Cuts at: 3274 3544 4433 6062 8
Size: 270 889 1629 43
Fragments arranged by size:
1629 1425 889 822 324 298 275 270
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3544 1537
Size: 275 1732 4090
Fragments arranged by size:
4090 1732 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2963 4027 1353
Size: 1610 1064 3423
Fragments arranged by size:
3423 1610 1064
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4684 5058 5204 387
Size: 1064 138 3095 374 146 1280
Fragments arranged by size:
3095 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2728 2728
Size: 6097
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2381 2594 2913 3070 4160 4757 5544
Size: 393 749 213 319 157 1090 597 787
Cuts at: 5544 5812 1239
Size: 268 1524
Fragments arranged by size:
1524 1090 787 749 597 393 319 268
213 157
EcoNI CCTnn'n_nnAGG
Cuts at: 2978 2978
Size: 6097
EcoO109I rG'GnC_Cy
Cuts at: 2767 2767
Size: 6097
EcoRI G'AATT_C
Cuts at: 3406 3784 3406
Size: 378 5719
FspI TGC'GCA
Cuts at: 4603 4603
Size: 6097
HaeII r_GCGC'y
Cuts at: 1678 5244 1678
Size: 3566 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2189 2221 2417 2429 2449
Size: 32 135 32 617 32 196 12 20
Cuts at: 2449 2461 2640 2672 2819 2851 4738 4770 4812
Size: 12 179 32 147 32 1887 32 42
Cuts at: 4812 4844 1373
Size: 32 2626
Fragments arranged by size:
2626 1887 617 196 179 147 135 42
32 32 32 32 32 32 32 20
12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2749 3441 3687 669
Size: 988 281 811 692 246 3079
Fragments arranged by size:
3079 988 811 692 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6097
HpaI GTT'AAC
Cuts at: 2749 3687 2749
Size: 938 5159
KpnI G_GTAC'C
Cuts at: 3422 3422
Size: 6097
MfeI C'AATT_G
Cuts at: 3696 3696
Size: 6097
MluI A'CGCG_T
Cuts at: 5973 5973
Size: 6097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5211 5395 1305
Size: 3906 184 2007
Fragments arranged by size:
3906 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2978 10
Size: 54 1425 343 1146 3129
Fragments arranged by size:
3129 1425 1146 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2147 2903 2939 4055 4414 4573 518
Size: 648 981 756 36 1116 359 159 2042
Fragments arranged by size:
2042 1116 981 756 648 359 159 36
MspA1I CmG'CkG
Cuts at: 1606 2137 3103 3248 3260 3513 4190 5338 5583
Size: 531 966 145 12 253 677 1148 245
Cuts at: 5583 1606
Size: 2120
Fragments arranged by size:
2120 1148 966 677 531 253 245 145
12
NcoI C'CATG_G
Cuts at: 513 1065 2940 513
Size: 552 1875 3670
Fragments arranged by size:
3670 1875 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6097
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6097
NotI GC'GGCC_GC
Cuts at: 3544 3544
Size: 6097
NsiI A_TGCA'T
Cuts at: 3202 3202
Size: 6097
NspI r_CATG'y
Cuts at: 1132 1229 3061 3455 5000 1132
Size: 97 1832 394 1545 2229
Fragments arranged by size:
2229 1832 1545 394 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6097
PciI A'CATG_T
Cuts at: 4996 4996
Size: 6097
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2617 1108
Size: 1509 4588
PfoI T'CCnGG_A
Cuts at: 2220 2220
Size: 6097
PmeI GTTT'AAAC
Cuts at: 3401 3401
Size: 6097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4166 4198 5679 5711 4166
Size: 32 1481 32 4552
Fragments arranged by size:
4552 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6097
PsiI TTA'TAA
Cuts at: 3335 3667 3335
Size: 332 5765
PstI C_TGCA'G
Cuts at: 830 3449 830
Size: 2619 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6097
PvuII CAG'CTG
Cuts at: 3103 3248 3103
Size: 145 5952
SacI G_AGCT'C
Cuts at: 2730 2730
Size: 6097
SacII CC_GC'GG
Cuts at: 2138 2138
Size: 6097
SalI G'TCGA_C
Cuts at: 1936 3439 1936
Size: 1503 4594
SapI GCTCTTCn'nnn_
Cuts at: 2963 2963
Size: 6097
SbfI CC_TGCA'GG
Cuts at: 3449 3449
Size: 6097
ScaI AGT'ACT
Cuts at: 4345 6057 4345
Size: 1712 4385
SfcI C'TryA_G
Cuts at: 826 1045 3445 4580 5261 5452 826
Size: 219 2400 1135 681 191 1471
Fragments arranged by size:
2400 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6097
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6097
SmaI CCC'GGG
Cuts at: 1872 3424 1872
Size: 1552 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2909 3218 4201 5102 5364 820
Size: 197 926 966 309 983 901 262 1553
Fragments arranged by size:
1553 983 966 926 901 309 262 197
SnaBI TAC'GTA
Cuts at: 493 3888 493
Size: 3395 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6097
SphI G_CATG'C
Cuts at: 1132 3455 1132
Size: 2323 3774
SspI AAT'ATT
Cuts at: 5 52 4021 5
Size: 47 3969 2081
Fragments arranged by size:
3969 2081 47
StuI AGG'CCT
Cuts at: 1517 3319 1517
Size: 1802 4295
StyI C'CwwG_G
Cuts at: 513 1065 2256 2940 2979 3536 513
Size: 552 1191 684 39 557 3074
Fragments arranged by size:
3074 1191 684 557 552 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2366 2656 2696 2915 4124 4141 4294
Size: 1255 786 290 40 219 1209 17 153
Cuts at: 4294 4479 4924 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1209 786 445 290 219 185
153 40 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4343 6055 96
Size: 274 80 33 51 1443 2366 1712 138
Fragments arranged by size:
2366 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6057 6089 6057
Size: 32 6065
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6097
XbaI T'CTAG_A
Cuts at: 3433 3433
Size: 6097
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6097
XmaI C'CCGG_G
Cuts at: 1870 3422 1870
Size: 1552 4545
XmnI GAAnn'nnTTC
Cuts at: 4226 4226
Size: 6097
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3905 276
Size: 53 83 186 936 2371 2468
Fragments arranged by size:
2468 2371 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI