Restriction map for
pFN21AE1924
(Circular) (Six-base) MAPSORT of: pFN21AE1924.seq Check: 2187 from: 1 to: 6088
pFN21AE1924 6088 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2369 2967 3158 2369
Size: 598 191 5299
Fragments arranged by size:
5299 598 191
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3898 278
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2821 3431 1937
Size: 884 610 4594
Fragments arranged by size:
4594 884 610
Acc65I G'GTAC_C
Cuts at: 3409 3409
Size: 6088
AclI AA'CG_TT
Cuts at: 1563 4215 4588 1563
Size: 2652 373 3063
Fragments arranged by size:
3063 2652 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2778 4151 5535 5803 1632
Size: 1146 1373 1384 268 1917
Fragments arranged by size:
1917 1384 1373 1146 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5891
AflIII A'CryG_T
Cuts at: 1203 4987 5964 1203
Size: 3784 977 1327
Fragments arranged by size:
3784 1327 977
AgeI A'CCGG_T
Cuts at: 4919 4919
Size: 6088
AhdI GACnn_n'nnGTC
Cuts at: 4817 4817
Size: 6088
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6088
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6088
AlwNI CAG_nnn'CTG
Cuts at: 2465 2959 3831 5403 2465
Size: 494 872 1572 3150
Fragments arranged by size:
3150 1572 872 494
ApaI G_GGCC'C
Cuts at: 2744 2945 2744
Size: 201 5887
ApaLI G'TGCA_C
Cuts at: 4145 5301 4145
Size: 1156 4932
ApoI r'AATT_y
Cuts at: 1493 3397 3623 3775 5884 1493
Size: 1904 226 152 2109 1697
Fragments arranged by size:
2109 1904 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4642 160
Size: 872 3610 1606
Fragments arranged by size:
3610 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
AvaI C'yCGr_G
Cuts at: 1870 1943 2111 2276 2337 2437 2500 2928 2945
Size: 73 168 165 61 100 63 428 17
Cuts at: 2945 3087 3413 1870
Size: 142 326 4545
Fragments arranged by size:
4545 428 326 168 165 142 100 73
63 61 17
BamHI G'GATC_C
Cuts at: 3418 3785 3418
Size: 367 5721
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2403 2902 3028 3052
Size: 325 217 627 221 395 499 126 24
Cuts at: 3052 3409 4864 618
Size: 357 1455 1842
Fragments arranged by size:
1842 1455 627 499 395 357 325 221
217 126 24
BanII G_rGCy'C
Cuts at: 1391 2052 2112 2744 2945 2981 3005 3107 3407
Size: 661 60 632 201 36 24 102 300
Cuts at: 3407 1391
Size: 4072
Fragments arranged by size:
4072 661 632 300 201 102 60 36
24
BbeI G_GCGC'C
Cuts at: 2012 3032 2012
Size: 1020 5068
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2191 928
Size: 971 292 4825
Fragments arranged by size:
4825 971 292
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4279 4313 1903
Size: 34 2342 34 3678
Fragments arranged by size:
3678 2342 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3980 5190 3980
Size: 1210 4878
BclI T'GATC_A
Cuts at: 1548 2964 1548
Size: 1416 4672
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2024 2033 2084
Size: 107 122 71 631 605 352 9 51
Cuts at: 2084 2439 2639 2924 3074 3083 4699 136
Size: 355 200 285 150 9 1616 1525
Fragments arranged by size:
1616 1525 631 605 355 352 285 200
150 122 107 71 51 9 9
BglII A'GATC_T
Cuts at: 2553 6083 2553
Size: 3530 2558
BlpI GC'TnA_GC
Cuts at: 2387 3505 2387
Size: 1118 4970
Bme1580I G_kGCm'C
Cuts at: 1790 2500 2744 2945 4149 5305 1790
Size: 710 244 201 1204 1156 2573
Fragments arranged by size:
2573 1204 1156 710 244 201
BmgBI CAC'GTC
Cuts at: 1318 2479 2607 2613 1318
Size: 1161 128 6 4793
Fragments arranged by size:
4793 1161 128 6
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2278 3378 3819
Size: 416 53 83 399 24 861 1100 441
Cuts at: 3819 4777 442
Size: 958 1753
Fragments arranged by size:
1753 1100 958 861 441 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6088
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2066 2679 2965 3017 3326 4748 1239
Size: 827 613 286 52 309 1422 2579
Fragments arranged by size:
2579 1422 827 613 309 286 52
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2305 2310 4213 5078 5376 2305
Size: 5 1903 865 298 3017
Fragments arranged by size:
3017 1903 865 298 5
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3311 4751 882
Size: 617 1812 1440 2219
Fragments arranged by size:
2219 1812 1440 617
BsaAI yAC'GTr
Cuts at: 493 1204 3879 493
Size: 711 2675 2702
Fragments arranged by size:
2702 2675 711
BsaBI GATnn'nnATC
Cuts at: 6082 6082
Size: 6088
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 3029 3311 3895
Size: 53 83 186 936 476 1020 282 584
Cuts at: 3895 4277 4912 275
Size: 382 635 1451
Fragments arranged by size:
1451 1020 936 635 584 476 382 282
186 83 53
BsaWI w'CCGG_w
Cuts at: 4521 4919 5193 5340 5751 4521
Size: 398 274 147 411 4858
Fragments arranged by size:
4858 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5863
Fragments arranged by size:
5863 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3187 1186
Size: 2001 4087
BseYI C'CCAG_C
Cuts at: 2256 2655 2702 2744 2752 3325 3371 5291 5787
Size: 399 47 42 8 573 46 1920 496
Cuts at: 5787 2256
Size: 2557
Fragments arranged by size:
2557 1920 573 496 399 47 46 42
8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2317 2341 2587 1163
Size: 573 581 24 246 4664
Fragments arranged by size:
4664 581 573 246 24
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3538 4299 5327 1540
Size: 275 186 1537 761 1028 2301
Fragments arranged by size:
2301 1537 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2052 2120 2768 2981 3005 3107 3197 3335 4149
Size: 68 648 213 24 102 90 138 814
Cuts at: 4149 4234 5305 2052
Size: 85 1071 2835
Fragments arranged by size:
2835 1071 814 648 213 138 102 90
85 68 24
BsmI GAATG_Cn'
Cuts at: 2587 3599 3692 2587
Size: 1012 93 4983
Fragments arranged by size:
4983 1012 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3302 3302
Size: 6088
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2112 2120 2500 2744 2768 2945
Size: 399 262 60 8 380 244 24 177
Cuts at: 2945 2981 3005 3107 3197 3335 3407 4149 4234
Size: 36 24 102 90 138 72 742 85
Cuts at: 4234 5305 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 380 262 244 177
138 102 90 85 72 60 36 24
24 8
BspEI T'CCGG_A
Cuts at: 5751 5751
Size: 6088
BspHI T'CATG_A
Cuts at: 2853 3977 5707 2853
Size: 1124 1730 3234
Fragments arranged by size:
3234 1730 1124
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2369 2967 3158 3253 3443 844
Size: 1525 598 191 95 190 3489
Fragments arranged by size:
3489 1525 598 191 190 95
BsrBI CCG'CTC
Cuts at: 1394 1823 3975 1394
Size: 429 2152 3507
Fragments arranged by size:
3507 2152 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4583 4757 65
Size: 1183 3335 174 1396
Fragments arranged by size:
3335 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2124 2406 4732 4919 1700
Size: 110 314 282 2326 187 2869
Fragments arranged by size:
2869 2326 314 282 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6088
BssHII G'CGCG_C
Cuts at: 1925 2382 2415 1925
Size: 457 33 5598
Fragments arranged by size:
5598 457 33
BssSI C'ACGA_G
Cuts at: 4148 5160 4148
Size: 1012 5076
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2071 2172 2959 794
Size: 1277 101 787 3923
Fragments arranged by size:
3923 1277 787 101
BstBI TT'CG_AA
Cuts at: 4892 4892
Size: 6088
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6088
BstXI CCAn_nnnn'nTGG
Cuts at: 3016 3016
Size: 6088
BstYI r'GATC_y
Cuts at: 1972 2553 3418 3785 4170 4187 5639 5725 5737
Size: 581 865 367 385 17 1452 86 12
Cuts at: 5737 6083 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 865 581 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2529 3127 513
Size: 552 939 525 598 3474
Fragments arranged by size:
3474 939 598 552 525
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5349
Fragments arranged by size:
5349 683 56
BtsI GCAGTG_nn'
Cuts at: 2706 3604 4398 4418 2706
Size: 898 794 20 4376
Fragments arranged by size:
4376 898 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6088
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6053
DraI TTT'AAA
Cuts at: 3392 3392
Size: 6088
DraIII CAC_nnn'GTG
Cuts at: 2226 2226
Size: 6088
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3263 5095 809
Size: 507 1947 1832 1802
Fragments arranged by size:
1947 1832 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2034 2451 2568
Size: 54 1425 50 275 18 204 417 117
Cuts at: 2568 3006 3124 3535 4424 6053 8
Size: 438 118 411 889 1629 43
Fragments arranged by size:
1629 1425 889 438 417 411 275 204
118 117 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3535 1537
Size: 275 1723 4090
Fragments arranged by size:
4090 1723 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2787 3333 4018 1353
Size: 1434 546 685 3423
Fragments arranged by size:
3423 1434 685 546
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4675 5049 5195 387
Size: 1064 138 3086 374 146 1280
Fragments arranged by size:
3086 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2050 2979 3003 3105 2050
Size: 929 24 102 5033
Fragments arranged by size:
5033 929 102 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2066 2679 2778 2965 3017 3326 4151
Size: 393 434 613 99 187 52 309 825
Cuts at: 4151 4748 5535 5803 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 825 787 613 597 434 393 309
268 187 99 52
EcoO109I rG'GnC_Cy
Cuts at: 2544 2741 2941 2942 3132 3265 2544
Size: 197 200 1 190 133 5367
Fragments arranged by size:
5367 200 197 190 133 1
EcoRI G'AATT_C
Cuts at: 3397 3775 3397
Size: 378 5710
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2893 2893
Size: 6088
FspI TGC'GCA
Cuts at: 2426 4594 2426
Size: 2168 3920
HaeII r_GCGC'y
Cuts at: 1678 2012 2975 3032 5235 1678
Size: 334 963 57 2203 2531
Fragments arranged by size:
2531 2203 963 334 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2034 2066 3170 3202 4729
Size: 32 135 32 462 32 1104 32 1527
Cuts at: 4729 4761 4803 4835 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1527 1104 462 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3432 3678 669
Size: 988 281 1494 246 3079
Fragments arranged by size:
3079 1494 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6088
HpaI GTT'AAC
Cuts at: 3678 3678
Size: 6088
KasI G'GCGC_C
Cuts at: 2008 3028 2008
Size: 1020 5068
KpnI G_GTAC'C
Cuts at: 3413 3413
Size: 6088
MfeI C'AATT_G
Cuts at: 3687 3687
Size: 6088
MluI A'CGCG_T
Cuts at: 5964 5964
Size: 6088
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2020 2538 2770 2997 5202 5386 1305
Size: 715 518 232 227 2205 184 2007
Fragments arranged by size:
2205 2007 715 518 232 227 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2453 2570 3126 10
Size: 54 1425 343 621 117 556 2972
Fragments arranged by size:
2972 1425 621 556 343 117 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4046 4405 4564 518
Size: 648 2880 359 159 2042
Fragments arranged by size:
2880 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2301 2422 2756 3304 3504 4181 5329 5574
Size: 695 121 334 548 200 677 1148 245
Cuts at: 5574 1606
Size: 2120
Fragments arranged by size:
2120 1148 695 677 548 334 245 200
121
NaeI GCC'GGC
Cuts at: 2126 2408 2126
Size: 282 5806
NarI GG'CG_CC
Cuts at: 2009 3029 2009
Size: 1020 5068
NcoI C'CATG_G
Cuts at: 513 1065 2004 3127 513
Size: 552 939 1123 3474
Fragments arranged by size:
3474 1123 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6088
NgoMIV G'CCGG_C
Cuts at: 2124 2406 2124
Size: 282 5806
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6088
NotI GC'GGCC_GC
Cuts at: 3535 3535
Size: 6088
NspI r_CATG'y
Cuts at: 1132 1229 2124 2192 3446 4991 1132
Size: 97 895 68 1254 1545 2229
Fragments arranged by size:
2229 1545 1254 895 97 68
PasI CC'CwG_GG
Cuts at: 1348 2576 2636 1348
Size: 1228 60 4800
Fragments arranged by size:
4800 1228 60
PciI A'CATG_T
Cuts at: 4987 4987
Size: 6088
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6088
PmeI GTTT'AAAC
Cuts at: 3392 3392
Size: 6088
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4157 4189 5670 5702 4157
Size: 32 1481 32 4543
Fragments arranged by size:
4543 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3132 3265 3132
Size: 133 5955
PshAI GACnn'nnGTC
Cuts at: 1613 3137 1613
Size: 1524 4564
PsiI TTA'TAA
Cuts at: 3658 3658
Size: 6088
PspOMI G'GGCC_C
Cuts at: 2740 2941 2740
Size: 201 5887
PstI C_TGCA'G
Cuts at: 830 2168 2366 2889 3440 830
Size: 1338 198 523 551 3478
Fragments arranged by size:
3478 1338 551 523 198
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6088
PvuII CAG'CTG
Cuts at: 2756 3304 2756
Size: 548 5540
SacI G_AGCT'C
Cuts at: 2052 2981 3005 3107 2052
Size: 929 24 102 5033
Fragments arranged by size:
5033 929 102 24
SalI G'TCGA_C
Cuts at: 1936 3430 1936
Size: 1494 4594
SanDI GG'GwC_CC
Cuts at: 3132 3132
Size: 6088
SapI GCTCTTCn'nnn_
Cuts at: 2787 2787
Size: 6088
SbfI CC_TGCA'GG
Cuts at: 2366 3440 2366
Size: 1074 5014
ScaI AGT'ACT
Cuts at: 4336 6048 4336
Size: 1712 4376
SexAI A'CCwGG_T
Cuts at: 2313 2313
Size: 6088
SfcI C'TryA_G
Cuts at: 826 1045 2164 2302 2362 2885 3166 3436 4571
Size: 219 1119 138 60 523 281 270 1135
Cuts at: 4571 5252 5443 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1119 681 523 281 270 219
191 138 60
SfiI GGCCn_nnn'nGGCC
Cuts at: 2033 3074 3083 2033
Size: 1041 9 5038
Fragments arranged by size:
5038 1041 9
SfoI GGC'GCC
Cuts at: 2010 3030 2010
Size: 1020 5068
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6088
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6088
SmaI CCC'GGG
Cuts at: 1872 2113 2439 2502 2930 3089 3415 1872
Size: 241 326 63 428 159 326 4545
Fragments arranged by size:
4545 428 326 326 241 159 63
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2284 2325 4192 5093 5355 820
Size: 197 926 341 41 1867 901 262 1553
Fragments arranged by size:
1867 1553 926 901 341 262 197 41
SnaBI TAC'GTA
Cuts at: 493 3879 493
Size: 3386 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6088
SphI G_CATG'C
Cuts at: 1132 3446 1132
Size: 2314 3774
SrfI GCCC'GGGC
Cuts at: 2439 3089 2439
Size: 650 5438
SspI AAT'ATT
Cuts at: 5 52 4012 5
Size: 47 3960 2081
Fragments arranged by size:
3960 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6088
StyI C'CwwG_G
Cuts at: 513 1065 2004 2814 2898 3127 3276 3355 3527
Size: 552 939 810 84 229 149 79 172
Cuts at: 3527 513
Size: 3074
Fragments arranged by size:
3074 939 810 552 229 172 149 84
79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2270 2792 3285 4115 4132 4285 4470
Size: 1255 690 522 493 830 17 153 185
Cuts at: 4470 4915 325
Size: 445 1498
Fragments arranged by size:
1498 1255 830 690 522 493 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2644 4334 6046
Size: 274 80 33 51 1443 667 1690 1712
Cuts at: 6046 96
Size: 138
Fragments arranged by size:
1712 1690 1443 667 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2210 2242 2784 2816 6048 6080 2210
Size: 32 542 32 3232 32 2218
Fragments arranged by size:
3232 2218 542 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6088
XbaI T'CTAG_A
Cuts at: 3424 3424
Size: 6088
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2626 3214 2626
Size: 588 5500
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6088
XmaI C'CCGG_G
Cuts at: 1870 2111 2437 2500 2928 3087 3413 1870
Size: 241 326 63 428 159 326 4545
Fragments arranged by size:
4545 428 326 326 241 159 63
XmnI GAAnn'nnTTC
Cuts at: 4217 4217
Size: 6088
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3896 276
Size: 53 83 186 936 2362 2468
Fragments arranged by size:
2468 2362 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI
NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII
SacII SwaI