Restriction map for
pFN21AE1923
(Circular) (Six-base) MAPSORT of: pFN21AE1923.seq Check: 803 from: 1 to: 6085
pFN21AE1923 6085 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3895 278
Size: 53 83 186 936 2359 2468
Fragments arranged by size:
2468 2359 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2820 3428 1937
Size: 883 608 4594
Fragments arranged by size:
4594 883 608
Acc65I G'GTAC_C
Cuts at: 3406 3406
Size: 6085
AclI AA'CG_TT
Cuts at: 1563 4212 4585 1563
Size: 2649 373 3063
Fragments arranged by size:
3063 2649 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2141 2675 4148 5532 5800 1632
Size: 509 534 1473 1384 268 1917
Fragments arranged by size:
1917 1473 1384 534 509 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5888
AflIII A'CryG_T
Cuts at: 1203 4984 5961 1203
Size: 3781 977 1327
Fragments arranged by size:
3781 1327 977
AgeI A'CCGG_T
Cuts at: 4916 4916
Size: 6085
AhdI GACnn_n'nnGTC
Cuts at: 4814 4814
Size: 6085
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6085
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6085
AlwNI CAG_nnn'CTG
Cuts at: 3828 5400 3828
Size: 1572 4513
ApaLI G'TGCA_C
Cuts at: 4142 5298 4142
Size: 1156 4929
ApoI r'AATT_y
Cuts at: 1493 2136 2397 2643 2939 3394 3620 3772 5881
Size: 643 261 246 296 455 226 152 2109
Cuts at: 5881 1493
Size: 1697
Fragments arranged by size:
2109 1697 643 455 296 261 246 226
152
AseI AT'TA_AT
Cuts at: 160 1032 2208 2855 4639 160
Size: 872 1176 647 1784 1606
Fragments arranged by size:
1784 1606 1176 872 647
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6085
AvaI C'yCGr_G
Cuts at: 1870 1943 3410 1870
Size: 73 1467 4545
Fragments arranged by size:
4545 1467 73
BamHI G'GATC_C
Cuts at: 3415 3782 3415
Size: 367 5718
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3406 4861 618
Size: 325 217 627 1619 1455 1842
Fragments arranged by size:
1842 1619 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3404 1391
Size: 2013 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2559 3018 928
Size: 971 660 459 3995
Fragments arranged by size:
3995 971 660 459
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4276 4310 1903
Size: 34 2339 34 3678
Fragments arranged by size:
3678 2339 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3977 5187 3977
Size: 1210 4875
BclI T'GATC_A
Cuts at: 1548 2062 2239 1548
Size: 514 177 5394
Fragments arranged by size:
5394 514 177
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4696 136
Size: 107 122 71 631 605 3024 1525
Fragments arranged by size:
3024 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6080 6080
Size: 6085
BlpI GC'TnA_GC
Cuts at: 3502 3502
Size: 6085
Bme1580I G_kGCm'C
Cuts at: 1790 4146 5302 1790
Size: 2356 1156 2573
Fragments arranged by size:
2573 2356 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6085
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3816 4774 442
Size: 416 53 83 399 24 2399 958 1753
Fragments arranged by size:
2399 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6085
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2038 2079 2454 2525 4745 1239
Size: 799 41 375 71 2220 2579
Fragments arranged by size:
2579 2220 799 375 71 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2790 4210 5075 5373 2790
Size: 1420 865 298 3502
Fragments arranged by size:
3502 1420 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4748 882
Size: 617 3249 2219
Fragments arranged by size:
3249 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3876 493
Size: 711 2672 2702
Fragments arranged by size:
2702 2672 711
BsaBI GATnn'nnATC
Cuts at: 6079 6079
Size: 6085
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3892 4274 4909 275
Size: 53 83 186 936 2359 382 635 1451
Fragments arranged by size:
2359 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4518 4916 5190 5337 5748 4518
Size: 398 274 147 411 4855
Fragments arranged by size:
4855 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5860
Fragments arranged by size:
5860 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3301 1186
Size: 2115 3970
BseYI C'CCAG_C
Cuts at: 2795 5288 5784 2795
Size: 2493 496 3096
Fragments arranged by size:
3096 2493 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5512
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3535 4296 5324 1540
Size: 275 186 1534 761 1028 2301
Fragments arranged by size:
2301 1534 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2671 4146 4231 5302 2671
Size: 1475 85 1071 3454
Fragments arranged by size:
3454 1475 1071 85
BsmI GAATG_Cn'
Cuts at: 2727 3596 3689 2727
Size: 869 93 5123
Fragments arranged by size:
5123 869 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2671 3404 4146 4231 5302 1391
Size: 399 881 733 742 85 1071 2174
Fragments arranged by size:
2174 1071 881 742 733 399 85
BspEI T'CCGG_A
Cuts at: 5748 5748
Size: 6085
BspHI T'CATG_A
Cuts at: 3974 5704 3974
Size: 1730 4355
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3440 844
Size: 2596 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3972 1394
Size: 429 2149 3507
Fragments arranged by size:
3507 2149 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3112 4580 4754 65
Size: 1183 1864 1468 174 1396
Fragments arranged by size:
1864 1468 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4729 4916 1700
Size: 110 2919 187 2869
Fragments arranged by size:
2919 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6085
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6085
BssSI C'ACGA_G
Cuts at: 4145 5157 4145
Size: 1012 5073
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6085
BstBI TT'CG_AA
Cuts at: 4889 4889
Size: 6085
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6085
BstYI r'GATC_y
Cuts at: 1972 3415 3782 4167 4184 5636 5722 5734 6080
Size: 1443 367 385 17 1452 86 12 346
Cuts at: 6080 1972
Size: 1977
Fragments arranged by size:
1977 1452 1443 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2547 2846 513
Size: 552 1482 299 3752
Fragments arranged by size:
3752 1482 552 299
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5346
Fragments arranged by size:
5346 683 56
BtsI GCAGTG_nn'
Cuts at: 3601 4395 4415 3601
Size: 794 20 5271
Fragments arranged by size:
5271 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6085
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6050
DraI TTT'AAA
Cuts at: 3389 3389
Size: 6085
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5092 809
Size: 507 3776 1802
Fragments arranged by size:
3776 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3532 4421 6050
Size: 54 1425 50 275 18 1702 889 1629
Cuts at: 6050 8
Size: 43
Fragments arranged by size:
1702 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3532 1537
Size: 275 1720 4090
Fragments arranged by size:
4090 1720 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2331 2424 3286 4015 1353
Size: 978 93 862 729 3423
Fragments arranged by size:
3423 978 862 729 93
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4672 5046 5192 387
Size: 1064 138 3083 374 146 1280
Fragments arranged by size:
3083 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2038 2079 2141 2454 2525 2675 4148
Size: 393 406 41 62 313 71 150 1473
Cuts at: 4148 4745 5532 5800 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1473 787 597 406 393 313 268
150 71 62 41
EcoRI G'AATT_C
Cuts at: 3394 3772 3394
Size: 378 5707
FspI TGC'GCA
Cuts at: 4591 4591
Size: 6085
HaeII r_GCGC'y
Cuts at: 1678 5232 1678
Size: 3554 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2060 2075 2090 2092 2107
Size: 32 135 32 488 15 15 2 15
Cuts at: 2107 2122 2819 2851 4726 4758 4800 4832 1373
Size: 15 697 32 1875 32 42 32 2626
Fragments arranged by size:
2626 1875 697 488 135 42 32 32
32 32 32 15 15 15 15 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3429 3675 669
Size: 988 281 1491 246 3079
Fragments arranged by size:
3079 1491 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3376 748
Size: 2628 3457
HpaI GTT'AAC
Cuts at: 3675 3675
Size: 6085
KpnI G_GTAC'C
Cuts at: 3410 3410
Size: 6085
MfeI C'AATT_G
Cuts at: 3684 3684
Size: 6085
MluI A'CGCG_T
Cuts at: 5961 5961
Size: 6085
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2988 3386 5199 5383 1305
Size: 1683 398 1813 184 2007
Fragments arranged by size:
2007 1813 1683 398 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4263
Fragments arranged by size:
4263 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2772 4043 4402 4561 518
Size: 648 1606 1271 359 159 2042
Fragments arranged by size:
2042 1606 1271 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3501 4178 5326 5571 1606
Size: 1895 677 1148 245 2120
Fragments arranged by size:
2120 1895 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2846 513
Size: 552 1781 3752
Fragments arranged by size:
3752 1781 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6085
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6085
NotI GC'GGCC_GC
Cuts at: 3532 3532
Size: 6085
NspI r_CATG'y
Cuts at: 1132 1229 2782 3443 4988 1132
Size: 97 1553 661 1545 2229
Fragments arranged by size:
2229 1553 1545 661 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6085
PciI A'CATG_T
Cuts at: 4984 4984
Size: 6085
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2548 1108
Size: 1440 4645
PfoI T'CCnGG_A
Cuts at: 3123 3123
Size: 6085
PmeI GTTT'AAAC
Cuts at: 3389 3389
Size: 6085
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4154 4186 5667 5699 4154
Size: 32 1481 32 4540
Fragments arranged by size:
4540 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6085
PsiI TTA'TAA
Cuts at: 2754 3655 2754
Size: 901 5184
PstI C_TGCA'G
Cuts at: 830 3437 830
Size: 2607 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6085
SalI G'TCGA_C
Cuts at: 1936 3427 1936
Size: 1491 4594
SapI GCTCTTCn'nnn_
Cuts at: 2424 2424
Size: 6085
SbfI CC_TGCA'GG
Cuts at: 3437 3437
Size: 6085
ScaI AGT'ACT
Cuts at: 4333 6045 4333
Size: 1712 4373
SfcI C'TryA_G
Cuts at: 826 1045 3433 4568 5249 5440 826
Size: 219 2388 1135 681 191 1471
Fragments arranged by size:
2388 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6085
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6085
SmaI CCC'GGG
Cuts at: 1872 3412 1872
Size: 1540 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2805 4189 5090 5352 820
Size: 197 926 862 1384 901 262 1553
Fragments arranged by size:
1553 1384 926 901 862 262 197
SnaBI TAC'GTA
Cuts at: 493 3876 493
Size: 3383 2702
SpeI A'CTAG_T
Cuts at: 152 2621 152
Size: 2469 3616
SphI G_CATG'C
Cuts at: 1132 3443 1132
Size: 2311 3774
SspI AAT'ATT
Cuts at: 5 52 2423 3044 4009 5
Size: 47 2371 621 965 2081
Fragments arranged by size:
2371 2081 965 621 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6085
StyI C'CwwG_G
Cuts at: 513 1065 2846 3524 513
Size: 552 1781 678 3074
Fragments arranged by size:
3074 1781 678 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2493 4112 4129 4282 4467 4912 325
Size: 1255 913 1619 17 153 185 445 1498
Fragments arranged by size:
1619 1498 1255 913 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4331 6043 96
Size: 274 80 33 51 1443 2354 1712 138
Fragments arranged by size:
2354 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6045 6077 6045
Size: 32 6053
Tth111I GACn'n_nGTC
Cuts at: 1316 2169 2183 1316
Size: 853 14 5218
Fragments arranged by size:
5218 853 14
XbaI T'CTAG_A
Cuts at: 3421 3421
Size: 6085
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6085
XmaI C'CCGG_G
Cuts at: 1870 3410 1870
Size: 1540 4545
XmnI GAAnn'nnTTC
Cuts at: 2397 4214 2397
Size: 1817 4268
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3893 276
Size: 53 83 186 936 2359 2468
Fragments arranged by size:
2468 2359 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI