Restriction map for
pFN21AE1918
(Circular) (Six-base) MAPSORT of: pFN21AE1918.seq Check: 5243 from: 1 to: 6067
pFN21AE1918 6067 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3877 278
Size: 53 83 186 936 2341 2468
Fragments arranged by size:
2468 2341 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3410 1937
Size: 1473 4594
Acc65I G'GTAC_C
Cuts at: 3388 3388
Size: 6067
AclI AA'CG_TT
Cuts at: 1563 4194 4567 1563
Size: 2631 373 3063
Fragments arranged by size:
3063 2631 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2730 3016 3119 4130 5514 5782 1632
Size: 1098 286 103 1011 1384 268 1917
Fragments arranged by size:
1917 1384 1098 1011 286 268 103
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5870
AflIII A'CryG_T
Cuts at: 1203 2700 4966 5943 1203
Size: 1497 2266 977 1327
Fragments arranged by size:
2266 1497 1327 977
AgeI A'CCGG_T
Cuts at: 4898 4898
Size: 6067
AhdI GACnn_n'nnGTC
Cuts at: 4796 4796
Size: 6067
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6067
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6067
AlwNI CAG_nnn'CTG
Cuts at: 3810 5382 3810
Size: 1572 4495
ApaI G_GGCC'C
Cuts at: 3005 3005
Size: 6067
ApaLI G'TGCA_C
Cuts at: 3355 4124 5280 3355
Size: 769 1156 4142
Fragments arranged by size:
4142 1156 769
ApoI r'AATT_y
Cuts at: 1493 2021 2615 2710 3376 3602 3754 5863 1493
Size: 528 594 95 666 226 152 2109 1697
Fragments arranged by size:
2109 1697 666 594 528 226 152 95
AseI AT'TA_AT
Cuts at: 160 1032 4621 160
Size: 872 3589 1606
Fragments arranged by size:
3589 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6067
AvaI C'yCGr_G
Cuts at: 1870 1943 3392 1870
Size: 73 1449 4545
Fragments arranged by size:
4545 1449 73
AvrII C'CTAG_G
Cuts at: 2234 2234
Size: 6067
BamHI G'GATC_C
Cuts at: 3397 3764 3397
Size: 367 5700
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3388 4843 618
Size: 325 217 627 1601 1455 1842
Fragments arranged by size:
1842 1601 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2584 3005 3069 3306 3386 1391
Size: 1193 421 64 237 80 4072
Fragments arranged by size:
4072 1193 421 237 80 64
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2550 928
Size: 971 651 4445
Fragments arranged by size:
4445 971 651
BbvCI CC'TCA_GC
Cuts at: 2925 2925
Size: 6067
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2554 2588 4258 4292 1903
Size: 34 617 34 1670 34 3678
Fragments arranged by size:
3678 1670 617 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3959 5169 3959
Size: 1210 4857
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6067
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4678 136
Size: 107 122 71 631 605 3006 1525
Fragments arranged by size:
3006 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2936 6062 2936
Size: 3126 2941
BlpI GC'TnA_GC
Cuts at: 3484 3484
Size: 6067
Bme1580I G_kGCm'C
Cuts at: 1790 3005 3359 4128 5284 1790
Size: 1215 354 769 1156 2573
Fragments arranged by size:
2573 1215 1156 769 354
BmgBI CAC'GTC
Cuts at: 1318 2012 1318
Size: 694 5373
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3798 4756 442
Size: 416 53 83 399 24 2381 958 1753
Fragments arranged by size:
2381 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6067
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2201 2201
Size: 6067
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2236 2406 2756 3177 4727 1239
Size: 997 170 350 421 1550 2579
Fragments arranged by size:
2579 1550 997 421 350 170
Bpu10I CC'TnA_GC
Cuts at: 2820 2925 2820
Size: 105 5962
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2450 3304 4192 5057 5355 2450
Size: 854 888 865 298 3162
Fragments arranged by size:
3162 888 865 854 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2382 4730 882
Size: 617 883 2348 2219
Fragments arranged by size:
2348 2219 883 617
BsaAI yAC'GTr
Cuts at: 493 1204 3858 493
Size: 711 2654 2702
Fragments arranged by size:
2702 2654 711
BsaBI GATnn'nnATC
Cuts at: 6061 6061
Size: 6067
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3874 4256 4891 275
Size: 53 83 186 936 2341 382 635 1451
Fragments arranged by size:
2341 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4500 4898 5172 5319 5730 4500
Size: 398 274 147 411 4837
Fragments arranged by size:
4837 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2379 2409 2540 2570 1178
Size: 30 165 30 976 30 131 30 4675
Fragments arranged by size:
4675 976 165 131 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3229 1186
Size: 2043 4024
BseYI C'CCAG_C
Cuts at: 3004 5270 5766 3004
Size: 2266 496 3305
Fragments arranged by size:
3305 2266 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5494
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3517 4278 5306 1540
Size: 275 186 1516 761 1028 2301
Fragments arranged by size:
2301 1516 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2584 2821 3069 3359 4128 4213 5284 2584
Size: 237 248 290 769 85 1071 3367
Fragments arranged by size:
3367 1071 769 290 248 237 85
BsmI GAATG_Cn'
Cuts at: 3578 3671 3578
Size: 93 5974
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2584 2821 3005 3069 3306 3359 3386
Size: 399 794 237 184 64 237 53 27
Cuts at: 3386 4128 4213 5284 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 794 742 399 237 237 184
85 64 53 27
BspEI T'CCGG_A
Cuts at: 5730 5730
Size: 6067
BspHI T'CATG_A
Cuts at: 3956 5686 3956
Size: 1730 4337
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3274 3422 844
Size: 2430 148 3489
Fragments arranged by size:
3489 2430 148
BsrBI CCG'CTC
Cuts at: 1394 1823 3954 1394
Size: 429 2131 3507
Fragments arranged by size:
3507 2131 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4562 4736 65
Size: 1183 3314 174 1396
Fragments arranged by size:
3314 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4711 4898 1700
Size: 110 2901 187 2869
Fragments arranged by size:
2901 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2481 96
Size: 2385 3682
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6067
BssSI C'ACGA_G
Cuts at: 4127 5139 4127
Size: 1012 5055
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6067
BstBI TT'CG_AA
Cuts at: 4871 4871
Size: 6067
BstEII G'GTnAC_C
Cuts at: 1184 2229 1184
Size: 1045 5022
BstYI r'GATC_y
Cuts at: 1972 2936 3397 3764 4149 4166 5618 5704 5716
Size: 964 461 367 385 17 1452 86 12
Cuts at: 5716 6062 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 964 461 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3204 513
Size: 552 939 1200 3376
Fragments arranged by size:
3376 1200 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5328
Fragments arranged by size:
5328 683 56
BtsI GCAGTG_nn'
Cuts at: 3583 4377 4397 3583
Size: 794 20 5253
Fragments arranged by size:
5253 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6067
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6032
DraI TTT'AAA
Cuts at: 3371 3371
Size: 6067
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2779 5074 809
Size: 507 1463 2295 1802
Fragments arranged by size:
2295 1802 1463 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3514 4403 6032
Size: 54 1425 50 275 18 1684 889 1629
Cuts at: 6032 8
Size: 43
Fragments arranged by size:
1684 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3514 1537
Size: 275 1702 4090
Fragments arranged by size:
4090 1702 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2357 2910 3997 1353
Size: 1004 553 1087 3423
Fragments arranged by size:
3423 1087 1004 553
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4654 5028 5174 387
Size: 1064 138 3065 374 146 1280
Fragments arranged by size:
3065 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2582 3067 2582
Size: 485 5582
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2236 2406 2730 2756 3016 3119 3177
Size: 393 604 170 324 26 260 103 58
Cuts at: 3177 4130 4727 5514 5782 1239
Size: 953 597 787 268 1524
Fragments arranged by size:
1524 953 787 604 597 393 324 268
260 170 103 58 26
EcoO109I rG'GnC_Cy
Cuts at: 2873 2873
Size: 6067
EcoRI G'AATT_C
Cuts at: 3376 3754 3376
Size: 378 5689
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2491 2880 3013 2491
Size: 389 133 5545
Fragments arranged by size:
5545 389 133
FspI TGC'GCA
Cuts at: 4573 4573
Size: 6067
HaeII r_GCGC'y
Cuts at: 1678 5214 1678
Size: 3536 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2111 2143 2540 2572 2921
Size: 32 135 32 539 32 397 32 349
Cuts at: 2921 2953 3106 3138 4708 4740 4782 4814 1373
Size: 32 153 32 1570 32 42 32 2626
Fragments arranged by size:
2626 1570 539 397 349 153 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3411 3657 669
Size: 988 281 1473 246 3079
Fragments arranged by size:
3079 1473 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6067
HpaI GTT'AAC
Cuts at: 3657 3657
Size: 6067
KpnI G_GTAC'C
Cuts at: 3392 3392
Size: 6067
MfeI C'AATT_G
Cuts at: 3315 3666 3315
Size: 351 5716
MluI A'CGCG_T
Cuts at: 5943 5943
Size: 6067
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2103 2706 3154 5181 5365 1305
Size: 798 603 448 2027 184 2007
Fragments arranged by size:
2027 2007 798 603 448 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4245
Fragments arranged by size:
4245 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2163 3183 4025 4384 4543 518
Size: 648 997 1020 842 359 159 2042
Fragments arranged by size:
2042 1020 997 842 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2734 2835 3097 3483 4160 5308 5553 1606
Size: 1128 101 262 386 677 1148 245 2120
Fragments arranged by size:
2120 1148 1128 677 386 262 245 101
NcoI C'CATG_G
Cuts at: 513 1065 2004 3204 513
Size: 552 939 1200 3376
Fragments arranged by size:
3376 1200 939 552
NdeI CA'TA_TG
Cuts at: 387 2290 387
Size: 1903 4164
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6067
NotI GC'GGCC_GC
Cuts at: 3514 3514
Size: 6067
NspI r_CATG'y
Cuts at: 1132 1229 2704 3425 4970 1132
Size: 97 1475 721 1545 2229
Fragments arranged by size:
2229 1545 1475 721 97
PasI CC'CwG_GG
Cuts at: 1348 2869 1348
Size: 1521 4546
PciI A'CATG_T
Cuts at: 2700 4966 2700
Size: 2266 3801
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6067
PfoI T'CCnGG_A
Cuts at: 2383 2383
Size: 6067
PmeI GTTT'AAAC
Cuts at: 3371 3371
Size: 6067
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2448 2480 4136 4168 5649 5681 2448
Size: 32 1656 32 1481 32 2834
Fragments arranged by size:
2834 1656 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2873 2873
Size: 6067
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6067
PsiI TTA'TAA
Cuts at: 3637 3637
Size: 6067
PspOMI G'GGCC_C
Cuts at: 3001 3001
Size: 6067
PstI C_TGCA'G
Cuts at: 830 2157 2244 3419 830
Size: 1327 87 1175 3478
Fragments arranged by size:
3478 1327 1175 87
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6067
PvuII CAG'CTG
Cuts at: 2734 2835 3097 2734
Size: 101 262 5704
Fragments arranged by size:
5704 262 101
SacI G_AGCT'C
Cuts at: 2584 3069 2584
Size: 485 5582
SalI G'TCGA_C
Cuts at: 1936 3409 1936
Size: 1473 4594
SapI GCTCTTCn'nnn_
Cuts at: 2357 2357
Size: 6067
SbfI CC_TGCA'GG
Cuts at: 3419 3419
Size: 6067
ScaI AGT'ACT
Cuts at: 4315 6027 4315
Size: 1712 4355
SexAI A'CCwGG_T
Cuts at: 2391 2391
Size: 6067
SfcI C'TryA_G
Cuts at: 826 1045 2153 2240 3415 4550 5231 5422 826
Size: 219 1108 87 1175 1135 681 191 1471
Fragments arranged by size:
1471 1175 1135 1108 681 219 191 87
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6067
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6067
SmaI CCC'GGG
Cuts at: 1872 3394 1872
Size: 1522 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2465 3283 4171 5072 5334 820
Size: 197 926 522 818 888 901 262 1553
Fragments arranged by size:
1553 926 901 888 818 522 262 197
SnaBI TAC'GTA
Cuts at: 493 3858 493
Size: 3365 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6067
SphI G_CATG'C
Cuts at: 1132 3425 1132
Size: 2293 3774
SspI AAT'ATT
Cuts at: 5 52 3991 5
Size: 47 3939 2081
Fragments arranged by size:
3939 2081 47
StuI AGG'CCT
Cuts at: 1517 2863 1517
Size: 1346 4721
StyI C'CwwG_G
Cuts at: 513 1065 2004 2071 2141 2234 2298 2997 3164
Size: 552 939 67 70 93 64 699 167
Cuts at: 3164 3204 3506 513
Size: 40 302 3074
Fragments arranged by size:
3074 939 699 552 302 167 93 70
67 64 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4094 4111 4264 4449 4894 325
Size: 1255 2514 17 153 185 445 1498
Fragments arranged by size:
2514 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2481 4313 6025
Size: 274 80 33 51 1443 504 1832 1712
Cuts at: 6025 96
Size: 138
Fragments arranged by size:
1832 1712 1443 504 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6027 6059 6027
Size: 32 6035
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6067
XbaI T'CTAG_A
Cuts at: 3403 3403
Size: 6067
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6067
XmaI C'CCGG_G
Cuts at: 1870 3392 1870
Size: 1522 4545
XmnI GAAnn'nnTTC
Cuts at: 2307 4196 2307
Size: 1889 4178
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3875 276
Size: 53 83 186 936 2341 2468
Fragments arranged by size:
2468 2341 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BfrBI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacII SanDI SfiI SfoI
SrfI SwaI XcmI