Restriction map for
pFN21AE1914
(Circular) (Six-base) MAPSORT of: pFN21AE1914.seq Check: 928 from: 1 to: 6058
pFN21AE1914 6058 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3868 278
Size: 53 83 186 936 2332 2468
Fragments arranged by size:
2468 2332 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3401 1937
Size: 1464 4594
Acc65I G'GTAC_C
Cuts at: 3379 3379
Size: 6058
AclI AA'CG_TT
Cuts at: 1563 4185 4558 1563
Size: 2622 373 3063
Fragments arranged by size:
3063 2622 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2488 2669 4121 5505 5773 1632
Size: 856 181 1452 1384 268 1917
Fragments arranged by size:
1917 1452 1384 856 268 181
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5861
AflIII A'CryG_T
Cuts at: 1203 3355 4957 5934 1203
Size: 2152 1602 977 1327
Fragments arranged by size:
2152 1602 1327 977
AgeI A'CCGG_T
Cuts at: 4889 4889
Size: 6058
AhdI GACnn_n'nnGTC
Cuts at: 4787 4787
Size: 6058
AleI CACnn'nnGTG
Cuts at: 1166 2373 2540 1166
Size: 1207 167 4684
Fragments arranged by size:
4684 1207 167
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6058
AlwNI CAG_nnn'CTG
Cuts at: 2992 3021 3801 5373 2992
Size: 29 780 1572 3677
Fragments arranged by size:
3677 1572 780 29
ApaLI G'TGCA_C
Cuts at: 3188 4115 5271 3188
Size: 927 1156 3975
Fragments arranged by size:
3975 1156 927
ApoI r'AATT_y
Cuts at: 1493 2497 3224 3367 3593 3745 5854 1493
Size: 1004 727 143 226 152 2109 1697
Fragments arranged by size:
2109 1697 1004 727 226 152 143
AseI AT'TA_AT
Cuts at: 160 1032 4612 160
Size: 872 3580 1606
Fragments arranged by size:
3580 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6058
AvaI C'yCGr_G
Cuts at: 1870 1943 2187 2731 3078 3383 1870
Size: 73 244 544 347 305 4545
Fragments arranged by size:
4545 544 347 305 244 73
BamHI G'GATC_C
Cuts at: 3388 3755 3388
Size: 367 5691
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3379 4834 618
Size: 325 217 627 1592 1455 1842
Fragments arranged by size:
1842 1592 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2254 2550 2854 2871 3377 1391
Size: 863 296 304 17 506 4072
Fragments arranged by size:
4072 863 506 304 296 17
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5087
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2032 2066 4249 4283 1903
Size: 34 50 34 11 34 2183 34 3678
Fragments arranged by size:
3678 2183 50 34 34 34 34 11
BciVI GTATCCnnnnn_n'
Cuts at: 3271 3950 5160 3271
Size: 679 1210 4169
Fragments arranged by size:
4169 1210 679
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6058
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2040 2397 3082
Size: 107 122 71 631 605 368 357 685
Cuts at: 3082 4669 136
Size: 1587 1525
Fragments arranged by size:
1587 1525 685 631 605 368 357 122
107 71
BglII A'GATC_T
Cuts at: 3132 6053 3132
Size: 2921 3137
BlpI GC'TnA_GC
Cuts at: 3086 3475 3086
Size: 389 5669
Bme1580I G_kGCm'C
Cuts at: 1790 2187 2432 2450 3192 4119 5275 1790
Size: 397 245 18 742 927 1156 2573
Fragments arranged by size:
2573 1156 927 742 397 245 18
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6058
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2314 3789 4747
Size: 416 53 83 399 24 897 1475 958
Cuts at: 4747 442
Size: 1753
Fragments arranged by size:
1753 1475 958 897 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6058
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3175 3175
Size: 6058
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 4718 1239
Size: 917 2562 2579
Fragments arranged by size:
2579 2562 917
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4183 5048 5346 4183
Size: 865 298 4895
Fragments arranged by size:
4895 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3151 4721 882
Size: 617 1652 1570 2219
Fragments arranged by size:
2219 1652 1570 617
BsaAI yAC'GTr
Cuts at: 493 1204 3849 493
Size: 711 2645 2702
Fragments arranged by size:
2702 2645 711
BsaBI GATnn'nnATC
Cuts at: 6052 6052
Size: 6058
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3865 4247 4882 275
Size: 53 83 186 936 2332 382 635 1451
Fragments arranged by size:
2332 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2487 2724 4491 4889 5163 5310 5721 2487
Size: 237 1767 398 274 147 411 2824
Fragments arranged by size:
2824 1767 411 398 274 237 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5833
Fragments arranged by size:
5833 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2489 3338 1186
Size: 1303 849 3906
Fragments arranged by size:
3906 1303 849
BseYI C'CCAG_C
Cuts at: 2102 2874 5261 5757 2102
Size: 772 2387 496 2403
Fragments arranged by size:
2403 2387 772 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2777 3023 1163
Size: 573 1041 246 4198
Fragments arranged by size:
4198 1041 573 246
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2113 3508 4269 5297 1540
Size: 275 186 112 1395 761 1028 2301
Fragments arranged by size:
2301 1395 1028 761 275 186 112
BsiHKAI G_wGCw'C
Cuts at: 2254 2871 3192 4119 4204 5275 2254
Size: 617 321 927 85 1071 3037
Fragments arranged by size:
3037 1071 927 617 321 85
BsmI GAATG_Cn'
Cuts at: 2229 2593 3569 3662 2229
Size: 364 976 93 4625
Fragments arranged by size:
4625 976 364 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2187 2254 2432 2450 2550 2854 2871
Size: 399 397 67 178 18 100 304 17
Cuts at: 2871 3192 3377 4119 4204 5275 1391
Size: 321 185 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 397 321 304 185
178 100 85 67 18 17
BspEI T'CCGG_A
Cuts at: 5721 5721
Size: 6058
BspHI T'CATG_A
Cuts at: 2457 2493 3947 5677 2457
Size: 36 1454 1730 2838
Fragments arranged by size:
2838 1730 1454 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2760 3413 844
Size: 1916 653 3489
Fragments arranged by size:
3489 1916 653
BsrBI CCG'CTC
Cuts at: 1394 1823 2706 3945 1394
Size: 429 883 1239 3507
Fragments arranged by size:
3507 1239 883 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4553 4727 65
Size: 1183 3305 174 1396
Fragments arranged by size:
3305 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4702 4889 1700
Size: 110 2892 187 2869
Fragments arranged by size:
2892 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6058
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6058
BssSI C'ACGA_G
Cuts at: 2369 4118 5130 2369
Size: 1749 1012 3297
Fragments arranged by size:
3297 1749 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6058
BstBI TT'CG_AA
Cuts at: 4862 4862
Size: 6058
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6058
BstXI CCAn_nnnn'nTGG
Cuts at: 2980 2980
Size: 6058
BstYI r'GATC_y
Cuts at: 1972 2674 3132 3388 3755 4140 4157 5609 5695
Size: 702 458 256 367 385 17 1452 86
Cuts at: 5695 5707 6053 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 702 458 385 367 346 256
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2113 2278 2859 2901 2923 513
Size: 552 1048 165 581 42 22 3648
Fragments arranged by size:
3648 1048 581 552 165 42 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5319
Fragments arranged by size:
5319 683 56
BtsI GCAGTG_nn'
Cuts at: 3574 4368 4388 3574
Size: 794 20 5244
Fragments arranged by size:
5244 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6058
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3088 3123 529
Size: 35 2524 35 3464
Fragments arranged by size:
3464 2524 35 35
DraI TTT'AAA
Cuts at: 3362 3362
Size: 6058
DraIII CAC_nnn'GTG
Cuts at: 2689 2689
Size: 6058
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2273 5065 809
Size: 507 957 2792 1802
Fragments arranged by size:
2792 1802 957 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2032 2862 2926
Size: 54 1425 50 275 18 202 830 64
Cuts at: 2926 3093 3120 3505 4394 6023 8
Size: 167 27 385 889 1629 43
Fragments arranged by size:
1629 1425 889 830 385 275 202 167
64 54 50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 3505 1537
Size: 275 1693 4090
Fragments arranged by size:
4090 1693 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3306 3988 1353
Size: 1953 682 3423
Fragments arranged by size:
3423 1953 682
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2909 4645 5019 5165 387
Size: 1064 138 1320 1736 374 146 1280
Fragments arranged by size:
1736 1320 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2252 2869 2252
Size: 617 5441
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2488 2669 4121 4718 5505 5773
Size: 393 524 332 181 1452 597 787 268
Cuts at: 5773 1239
Size: 1524
Fragments arranged by size:
1524 1452 787 597 524 393 332 268
181
EcoNI CCTnn'n_nnAGG
Cuts at: 2170 2170
Size: 6058
EcoO109I rG'GnC_Cy
Cuts at: 2118 2620 2118
Size: 502 5556
EcoRI G'AATT_C
Cuts at: 2497 3367 3745 2497
Size: 870 378 4810
Fragments arranged by size:
4810 870 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3198 3198
Size: 6058
FspI TGC'GCA
Cuts at: 2756 4564 2756
Size: 1808 4250
HaeII r_GCGC'y
Cuts at: 1678 5205 1678
Size: 3527 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2434 2466 3245 3277 4699
Size: 32 135 32 862 32 779 32 1422
Cuts at: 4699 4731 4773 4805 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1422 862 779 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3402 3648 669
Size: 988 281 1464 246 3079
Fragments arranged by size:
3079 1464 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3180 748
Size: 2432 3626
HpaI GTT'AAC
Cuts at: 3648 3648
Size: 6058
KpnI G_GTAC'C
Cuts at: 3383 3383
Size: 6058
MfeI C'AATT_G
Cuts at: 3657 3657
Size: 6058
MluI A'CGCG_T
Cuts at: 5934 5934
Size: 6058
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2511 5172 5356 1305
Size: 1206 2661 184 2007
Fragments arranged by size:
2661 2007 1206 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2034 10
Size: 54 1425 343 202 4034
Fragments arranged by size:
4034 1425 343 202 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2373 2540 4016 4375 4534 518
Size: 648 1207 167 1476 359 159 2042
Fragments arranged by size:
2042 1476 1207 648 359 167 159
MspA1I CmG'CkG
Cuts at: 1606 2115 2134 2578 3021 3474 4151 5299 5544
Size: 509 19 444 443 453 677 1148 245
Cuts at: 5544 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 509 453 444 443 245
19
NcoI C'CATG_G
Cuts at: 513 1065 2278 2901 513
Size: 552 1213 623 3670
Fragments arranged by size:
3670 1213 623 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6058
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6058
NotI GC'GGCC_GC
Cuts at: 3505 3505
Size: 6058
NspI r_CATG'y
Cuts at: 1132 1229 3359 3416 4961 1132
Size: 97 2130 57 1545 2229
Fragments arranged by size:
2229 2130 1545 97 57
PasI CC'CwG_GG
Cuts at: 1348 2419 2830 1348
Size: 1071 411 4576
Fragments arranged by size:
4576 1071 411
PciI A'CATG_T
Cuts at: 3355 4957 3355
Size: 1602 4456
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2064 2419 3256 1108
Size: 956 355 837 3910
Fragments arranged by size:
3910 956 837 355
PfoI T'CCnGG_A
Cuts at: 2438 2438
Size: 6058
PmeI GTTT'AAAC
Cuts at: 3362 3362
Size: 6058
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4127 4159 5640 5672 4127
Size: 32 1481 32 4513
Fragments arranged by size:
4513 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6058
PsiI TTA'TAA
Cuts at: 3628 3628
Size: 6058
PstI C_TGCA'G
Cuts at: 830 2397 3410 830
Size: 1567 1013 3478
Fragments arranged by size:
3478 1567 1013
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6058
PvuII CAG'CTG
Cuts at: 2134 2578 3021 2134
Size: 444 443 5171
Fragments arranged by size:
5171 444 443
RsrII CG'GwC_CG
Cuts at: 2485 2485
Size: 6058
SacI G_AGCT'C
Cuts at: 2254 2871 2254
Size: 617 5441
SacII CC_GC'GG
Cuts at: 2116 2116
Size: 6058
SalI G'TCGA_C
Cuts at: 1936 3400 1936
Size: 1464 4594
SbfI CC_TGCA'GG
Cuts at: 2397 3410 2397
Size: 1013 5045
ScaI AGT'ACT
Cuts at: 4306 6018 4306
Size: 1712 4346
SfcI C'TryA_G
Cuts at: 826 1045 2393 3406 4541 5222 5413 826
Size: 219 1348 1013 1135 681 191 1471
Fragments arranged by size:
1471 1348 1135 1013 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6058
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6058
SmaI CCC'GGG
Cuts at: 1872 2733 3080 3385 1872
Size: 861 347 305 4545
Fragments arranged by size:
4545 861 347 305
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4162 5063 5325 820
Size: 197 926 2219 901 262 1553
Fragments arranged by size:
2219 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3849 493
Size: 3356 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6058
SphI G_CATG'C
Cuts at: 1132 3416 1132
Size: 2284 3774
SspI AAT'ATT
Cuts at: 5 52 3982 5
Size: 47 3930 2081
Fragments arranged by size:
3930 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6058
StyI C'CwwG_G
Cuts at: 513 1065 2278 2523 2901 2973 3497 513
Size: 552 1213 245 378 72 524 3074
Fragments arranged by size:
3074 1213 552 524 378 245 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2677 2888 4085 4102 4255 4440 4885
Size: 1255 1097 211 1197 17 153 185 445
Cuts at: 4885 325
Size: 1498
Fragments arranged by size:
1498 1255 1197 1097 445 211 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2151 4304 6016
Size: 274 80 33 51 1443 174 2153 1712
Cuts at: 6016 96
Size: 138
Fragments arranged by size:
2153 1712 1443 274 174 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2675 2707 6018 6050 2675
Size: 32 3311 32 2683
Fragments arranged by size:
3311 2683 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6058
XbaI T'CTAG_A
Cuts at: 3298 3394 3298
Size: 96 5962
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2027 2285 2977 3208 2027
Size: 258 692 231 4877
Fragments arranged by size:
4877 692 258 231
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6058
XmaI C'CCGG_G
Cuts at: 1870 2731 3078 3383 1870
Size: 861 347 305 4545
Fragments arranged by size:
4545 861 347 305
XmnI GAAnn'nnTTC
Cuts at: 2501 4187 2501
Size: 1686 4372
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3866 276
Size: 53 83 186 936 2332 2468
Fragments arranged by size:
2468 2332 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII RsrII SacI SacII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI SanDI SapI
SexAI SfiI SfoI SrfI SwaI