Restriction map for
pFN21AE1913
(Circular) (Six-base) MAPSORT of: pFN21AE1913.seq Check: 5362 from: 1 to: 6055
pFN21AE1913 6055 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3865 278
Size: 53 83 186 936 2329 2468
Fragments arranged by size:
2468 2329 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2151 3398 1937
Size: 214 1247 4594
Fragments arranged by size:
4594 1247 214
Acc65I G'GTAC_C
Cuts at: 3376 3376
Size: 6055
AclI AA'CG_TT
Cuts at: 1563 4182 4555 1563
Size: 2619 373 3063
Fragments arranged by size:
3063 2619 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2242 2488 4118 5502 5770 1632
Size: 610 246 1630 1384 268 1917
Fragments arranged by size:
1917 1630 1384 610 268 246
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5858
AflIII A'CryG_T
Cuts at: 1203 4954 5931 1203
Size: 3751 977 1327
Fragments arranged by size:
3751 1327 977
AgeI A'CCGG_T
Cuts at: 4886 4886
Size: 6055
AhdI GACnn_n'nnGTC
Cuts at: 3259 4784 3259
Size: 1525 4530
AleI CACnn'nnGTG
Cuts at: 1166 2540 1166
Size: 1374 4681
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6055
AlwNI CAG_nnn'CTG
Cuts at: 2995 3133 3798 5370 2995
Size: 138 665 1572 3680
Fragments arranged by size:
3680 1572 665 138
ApaI G_GGCC'C
Cuts at: 2854 3350 2854
Size: 496 5559
ApaLI G'TGCA_C
Cuts at: 2018 4112 5268 2018
Size: 2094 1156 2805
Fragments arranged by size:
2805 2094 1156
ApoI r'AATT_y
Cuts at: 1493 3364 3590 3742 5851 1493
Size: 1871 226 152 2109 1697
Fragments arranged by size:
2109 1871 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4609 160
Size: 872 3577 1606
Fragments arranged by size:
3577 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
AvaI C'yCGr_G
Cuts at: 1870 1943 2187 2731 2829 2864 3380 1870
Size: 73 244 544 98 35 516 4545
Fragments arranged by size:
4545 544 516 244 98 73 35
BamHI G'GATC_C
Cuts at: 3036 3385 3752 3036
Size: 349 367 5339
Fragments arranged by size:
5339 367 349
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2776 3376 4831 618
Size: 325 217 627 989 600 1455 1842
Fragments arranged by size:
1842 1455 989 627 600 325 217
BanII G_rGCy'C
Cuts at: 1391 2438 2550 2854 2871 3350 3374 1391
Size: 1047 112 304 17 479 24 4072
Fragments arranged by size:
4072 1047 479 304 112 24 17
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3324 928
Size: 971 1425 3659
Fragments arranged by size:
3659 1425 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2032 2066 3007 3041 4246 4280 1903
Size: 34 95 34 941 34 1205 34 3678
Fragments arranged by size:
3678 1205 941 95 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2490 3947 5157 2490
Size: 1457 1210 3388
Fragments arranged by size:
3388 1457 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6055
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2040 2397 4666
Size: 107 122 71 631 605 368 357 2269
Cuts at: 4666 136
Size: 1525
Fragments arranged by size:
2269 1525 631 605 368 357 122 107
71
BglII A'GATC_T
Cuts at: 6050 6050
Size: 6055
BlpI GC'TnA_GC
Cuts at: 3472 3472
Size: 6055
Bme1580I G_kGCm'C
Cuts at: 1790 2022 2450 2517 2781 2854 2865 3350 4116
Size: 232 428 67 264 73 11 485 766
Cuts at: 4116 5272 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 766 485 428 264 232 73
67 11
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6055
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2314 3786 4744
Size: 416 53 83 399 24 897 1472 958
Cuts at: 4744 442
Size: 1753
Fragments arranged by size:
1753 1472 958 897 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6055
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 4715 1239
Size: 917 2559 2579
Fragments arranged by size:
2579 2559 917
Bpu10I CC'TnA_GC
Cuts at: 2236 2236
Size: 6055
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2633 3071 4180 5045 5343 2633
Size: 438 1109 865 298 3345
Fragments arranged by size:
3345 1109 865 438 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2591 4718 882
Size: 617 1092 2127 2219
Fragments arranged by size:
2219 2127 1092 617
BsaAI yAC'GTr
Cuts at: 493 1204 2113 2182 3846 493
Size: 711 909 69 1664 2702
Fragments arranged by size:
2702 1664 909 711 69
BsaBI GATnn'nnATC
Cuts at: 6049 6049
Size: 6055
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3285 3862 4244 4879
Size: 53 83 186 936 1752 577 382 635
Cuts at: 4879 275
Size: 1451
Fragments arranged by size:
1752 1451 936 635 577 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4488 4886 5160 5307 5718 4488
Size: 398 274 147 411 4825
Fragments arranged by size:
4825 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5830
Fragments arranged by size:
5830 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2489 3341 3344 1186
Size: 1303 852 3 3897
Fragments arranged by size:
3897 1303 852 3
BseYI C'CCAG_C
Cuts at: 2099 2419 2874 5258 5754 2099
Size: 320 455 2384 496 2400
Fragments arranged by size:
2400 2384 496 455 320
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2777 3173 1163
Size: 573 1041 396 4045
Fragments arranged by size:
4045 1041 573 396
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2488 2929 3294 3505 4266 5294
Size: 275 186 487 441 365 211 761 1028
Cuts at: 5294 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 487 441 365 275 211
186
BsiHKAI G_wGCw'C
Cuts at: 2022 2871 4116 4201 5272 2022
Size: 849 1245 85 1071 2805
Fragments arranged by size:
2805 1245 1071 849 85
BsmI GAATG_Cn'
Cuts at: 3566 3659 3566
Size: 93 5962
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2438 2450 2517 2550 2781 2854
Size: 399 232 416 12 67 33 231 73
Cuts at: 2854 2865 2871 3350 3374 4116 4201 5272 1391
Size: 11 6 479 24 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 479 416 399 232 231
85 73 67 33 24 12 11 6
BspEI T'CCGG_A
Cuts at: 5718 5718
Size: 6055
BspHI T'CATG_A
Cuts at: 2493 3944 5674 2493
Size: 1451 1730 2874
Fragments arranged by size:
2874 1730 1451
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2760 3410 844
Size: 1916 650 3489
Fragments arranged by size:
3489 1916 650
BsrBI CCG'CTC
Cuts at: 1394 1823 2140 2235 2706 3942 1394
Size: 429 317 95 471 1236 3507
Fragments arranged by size:
3507 1236 471 429 317 95
BsrDI GCAATG_nn'
Cuts at: 65 1248 4550 4724 65
Size: 1183 3302 174 1396
Fragments arranged by size:
3302 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2030 2294 4699 4886 1700
Size: 110 220 264 2405 187 2869
Fragments arranged by size:
2869 2405 264 220 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6055
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6055
BssSI C'ACGA_G
Cuts at: 4115 5127 4115
Size: 1012 5043
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6055
BstBI TT'CG_AA
Cuts at: 4859 4859
Size: 6055
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6055
BstXI CCAn_nnnn'nTGG
Cuts at: 2200 2200
Size: 6055
BstYI r'GATC_y
Cuts at: 1972 3036 3385 3752 4137 4154 5606 5692 5704
Size: 1064 349 367 385 17 1452 86 12
Cuts at: 5704 6050 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1064 385 367 349 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2220 2923 3079 3288 513
Size: 552 1155 703 156 209 3280
Fragments arranged by size:
3280 1155 703 552 209 156
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5316
Fragments arranged by size:
5316 683 56
BtsI GCAGTG_nn'
Cuts at: 3571 4365 4385 3571
Size: 794 20 5241
Fragments arranged by size:
5241 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6055
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6020
DraI TTT'AAA
Cuts at: 3359 3359
Size: 6055
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5062 809
Size: 507 3746 1802
Fragments arranged by size:
3746 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2032 2655 2694
Size: 54 1425 50 275 18 202 623 39
Cuts at: 2694 2763 2904 2926 2943 2997 3057 3120 3162
Size: 69 141 22 17 54 60 63 42
Cuts at: 3162 3291 3502 4391 6020 8
Size: 129 211 889 1629 43
Fragments arranged by size:
1629 1425 889 623 275 211 202 141
129 69 63 60 54 54 50 43
42 39 22 18 17
EagI C'GGCC_G
Cuts at: 1537 1812 2926 3291 3502 1537
Size: 275 1114 365 211 4090
Fragments arranged by size:
4090 1114 365 275 211
EarI CTCTTCn'nnn_
Cuts at: 1353 2173 2261 3294 3985 1353
Size: 820 88 1033 691 3423
Fragments arranged by size:
3423 1033 820 691 88
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2348 4642 5016 5162 387
Size: 1064 138 759 2294 374 146 1280
Fragments arranged by size:
2294 1280 1064 759 374 146 138
EcoICRI GAG'CTC
Cuts at: 2869 2869
Size: 6055
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2242 2488 4118 4715 5502 5770
Size: 393 524 86 246 1630 597 787 268
Cuts at: 5770 1239
Size: 1524
Fragments arranged by size:
1630 1524 787 597 524 393 268 246
86
EcoO109I rG'GnC_Cy
Cuts at: 2241 2674 2851 3346 3347 2241
Size: 433 177 495 1 4949
Fragments arranged by size:
4949 495 433 177 1
EcoRI G'AATT_C
Cuts at: 3364 3742 3364
Size: 378 5677
FseI GG_CCGG'CC
Cuts at: 2034 2034
Size: 6055
FspI TGC'GCA
Cuts at: 2756 4561 2756
Size: 1805 4250
HaeII r_GCGC'y
Cuts at: 1678 2624 5202 1678
Size: 946 2578 2531
Fragments arranged by size:
2578 2531 946
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3245 3277 3317 3349 4696
Size: 32 135 32 1673 32 40 32 1347
Cuts at: 4696 4728 4770 4802 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1673 1347 135 42 40 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2770 3399 3645 669
Size: 988 281 832 629 246 3079
Fragments arranged by size:
3079 988 832 629 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6055
HpaI GTT'AAC
Cuts at: 3645 3645
Size: 6055
KpnI G_GTAC'C
Cuts at: 3380 3380
Size: 6055
MfeI C'AATT_G
Cuts at: 3654 3654
Size: 6055
MluI A'CGCG_T
Cuts at: 5931 5931
Size: 6055
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3315 5169 5353 1305
Size: 2010 1854 184 2007
Fragments arranged by size:
2010 2007 1854 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2657 2945 2999 10
Size: 54 1425 343 825 288 54 3066
Fragments arranged by size:
3066 1425 825 343 288 54 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2198 2540 2969 3164 4013 4372 4531
Size: 648 1032 342 429 195 849 359 159
Cuts at: 4531 518
Size: 2042
Fragments arranged by size:
2042 1032 849 648 429 359 342 195
159
MspA1I CmG'CkG
Cuts at: 1606 2103 2134 2407 2578 3081 3471 4148 5296
Size: 497 31 273 171 503 390 677 1148
Cuts at: 5296 5541 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 503 497 390 273 245
171 31
NaeI GCC'GGC
Cuts at: 2032 2296 2032
Size: 264 5791
NcoI C'CATG_G
Cuts at: 513 1065 2220 513
Size: 552 1155 4348
Fragments arranged by size:
4348 1155 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6055
NgoMIV G'CCGG_C
Cuts at: 2030 2294 2030
Size: 264 5791
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6055
NotI GC'GGCC_GC
Cuts at: 3502 3502
Size: 6055
NspI r_CATG'y
Cuts at: 1132 1229 2968 3413 4958 1132
Size: 97 1739 445 1545 2229
Fragments arranged by size:
2229 1739 1545 445 97
PasI CC'CwG_GG
Cuts at: 1348 3343 1348
Size: 1995 4060
PciI A'CATG_T
Cuts at: 4954 4954
Size: 6055
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2064 2419 2578 2581 1108
Size: 956 355 159 3 4582
Fragments arranged by size:
4582 956 355 159 3
PfoI T'CCnGG_A
Cuts at: 2352 3126 2352
Size: 774 5281
PmeI GTTT'AAAC
Cuts at: 3359 3359
Size: 6055
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3209 3241 4124 4156 5637 5669 3209
Size: 32 883 32 1481 32 3595
Fragments arranged by size:
3595 1481 883 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2674 2674
Size: 6055
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6055
PsiI TTA'TAA
Cuts at: 3625 3625
Size: 6055
PspOMI G'GGCC_C
Cuts at: 2850 3346 2850
Size: 496 5559
PspXI vC'TCGA_Gb
Cuts at: 2187 2187
Size: 6055
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3005 3037 3005
Size: 32 6023
PstI C_TGCA'G
Cuts at: 830 2397 3407 830
Size: 1567 1010 3478
Fragments arranged by size:
3478 1567 1010
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6055
PvuII CAG'CTG
Cuts at: 2134 2407 2578 2134
Size: 273 171 5611
Fragments arranged by size:
5611 273 171
SacI G_AGCT'C
Cuts at: 2871 2871
Size: 6055
SacII CC_GC'GG
Cuts at: 3082 3082
Size: 6055
SalI G'TCGA_C
Cuts at: 1936 3397 1936
Size: 1461 4594
SbfI CC_TGCA'GG
Cuts at: 2397 3407 2397
Size: 1010 5045
ScaI AGT'ACT
Cuts at: 4303 6015 4303
Size: 1712 4343
SexAI A'CCwGG_T
Cuts at: 2685 3255 2685
Size: 570 5485
SfcI C'TryA_G
Cuts at: 826 1045 2393 3403 4538 5219 5410 826
Size: 219 1348 1010 1135 681 191 1471
Fragments arranged by size:
1471 1348 1135 1010 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6055
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6055
SmaI CCC'GGG
Cuts at: 1872 2733 2831 3382 1872
Size: 861 98 551 4545
Fragments arranged by size:
4545 861 551 98
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2187 2648 3086 4159 5060 5322
Size: 197 926 244 461 438 1073 901 262
Cuts at: 5322 820
Size: 1553
Fragments arranged by size:
1553 1073 926 901 461 438 262 244
197
SnaBI TAC'GTA
Cuts at: 493 3846 493
Size: 3353 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6055
SphI G_CATG'C
Cuts at: 1132 3413 1132
Size: 2281 3774
SspI AAT'ATT
Cuts at: 5 52 3979 5
Size: 47 3927 2081
Fragments arranged by size:
3927 2081 47
StuI AGG'CCT
Cuts at: 1517 2165 2263 3182 1517
Size: 648 98 919 4390
Fragments arranged by size:
4390 919 648 98
StyI C'CwwG_G
Cuts at: 513 1065 2220 2310 2523 3351 3494 513
Size: 552 1155 90 213 828 143 3074
Fragments arranged by size:
3074 1155 828 552 213 143 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2338 2536 2888 4082 4099 4252 4437
Size: 1255 758 198 352 1194 17 153 185
Cuts at: 4437 4882 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1194 758 445 352 198 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4301 6013 96
Size: 274 80 33 51 1443 2324 1712 138
Fragments arranged by size:
2324 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2359 2391 6015 6047 2359
Size: 32 3624 32 2367
Fragments arranged by size:
3624 2367 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2228 1316
Size: 912 5143
XbaI T'CTAG_A
Cuts at: 3391 3391
Size: 6055
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2575 2899 2977 2575
Size: 324 78 5653
Fragments arranged by size:
5653 324 78
XhoI C'TCGA_G
Cuts at: 1943 2187 1943
Size: 244 5811
XmaI C'CCGG_G
Cuts at: 1870 2731 2829 3380 1870
Size: 861 98 551 4545
Fragments arranged by size:
4545 861 551 98
XmnI GAAnn'nnTTC
Cuts at: 2501 4184 2501
Size: 1683 4372
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3863 276
Size: 53 83 186 936 2329 2468
Fragments arranged by size:
2468 2329 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII SacI SacII SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FspAI KasI NarI NruI NsiI PacI
PmlI RsrII SanDI SapI SfiI SfoI SrfI SwaI