Restriction map for
pFN21AE1911
(Circular) (Six-base) MAPSORT of: pFN21AE1911.seq Check: 5910 from: 1 to: 6040
pFN21AE1911 6040 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2726 2726
Size: 6040
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3850 278
Size: 53 83 186 936 2314 2468
Fragments arranged by size:
2468 2314 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3383 1937
Size: 1446 4594
Acc65I G'GTAC_C
Cuts at: 2447 3361 2447
Size: 914 5126
AclI AA'CG_TT
Cuts at: 1563 4167 4540 1563
Size: 2604 373 3063
Fragments arranged by size:
3063 2604 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2623 4103 5487 5755 1632
Size: 991 1480 1384 268 1917
Fragments arranged by size:
1917 1480 1384 991 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5843
AflIII A'CryG_T
Cuts at: 1203 2148 2499 4939 5916 1203
Size: 945 351 2440 977 1327
Fragments arranged by size:
2440 1327 977 945 351
AgeI A'CCGG_T
Cuts at: 4871 4871
Size: 6040
AhdI GACnn_n'nnGTC
Cuts at: 3259 4769 3259
Size: 1510 4530
AleI CACnn'nnGTG
Cuts at: 1166 2540 1166
Size: 1374 4666
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2198 1184
Size: 1014 5026
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3293 3325 3293
Size: 32 6008
AlwNI CAG_nnn'CTG
Cuts at: 3133 3783 5355 3133
Size: 650 1572 3818
Fragments arranged by size:
3818 1572 650
ApaI G_GGCC'C
Cuts at: 2244 2244
Size: 6040
ApaLI G'TGCA_C
Cuts at: 2018 4097 5253 2018
Size: 2079 1156 2805
Fragments arranged by size:
2805 2079 1156
ApoI r'AATT_y
Cuts at: 1493 3349 3575 3727 5836 1493
Size: 1856 226 152 2109 1697
Fragments arranged by size:
2109 1856 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4594 160
Size: 872 3562 1606
Fragments arranged by size:
3562 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6040
AvaI C'yCGr_G
Cuts at: 1870 1943 2236 2864 3078 3365 1870
Size: 73 293 628 214 287 4545
Fragments arranged by size:
4545 628 293 287 214 73
BamHI G'GATC_C
Cuts at: 3036 3370 3737 3036
Size: 334 367 5339
Fragments arranged by size:
5339 367 334
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2054 2105 2216 2447 2512
Size: 325 217 627 267 51 111 231 65
Cuts at: 2512 3361 4816 618
Size: 849 1455 1842
Fragments arranged by size:
1842 1455 849 627 325 267 231 217
111 65 51
BanII G_rGCy'C
Cuts at: 1391 2214 2244 2550 2871 3359 1391
Size: 823 30 306 321 488 4072
Fragments arranged by size:
4072 823 488 321 306 30
BbeI G_GCGC'C
Cuts at: 2058 2058
Size: 6040
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5069
BbvCI CC'TCA_GC
Cuts at: 3324 3324
Size: 6040
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2032 2066 4231 4265 1903
Size: 34 95 34 2165 34 3678
Fragments arranged by size:
3678 2165 95 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3932 5142 3932
Size: 1210 4830
BclI T'GATC_A
Cuts at: 1548 2073 1548
Size: 525 5515
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2248 4651 136
Size: 107 122 71 631 605 576 2403 1525
Fragments arranged by size:
2403 1525 631 605 576 122 107 71
BglII A'GATC_T
Cuts at: 2253 6035 2253
Size: 3782 2258
BlpI GC'TnA_GC
Cuts at: 3457 3457
Size: 6040
Bme1580I G_kGCm'C
Cuts at: 1790 2022 2108 2187 2244 2319 2865 4101 5257
Size: 232 86 79 57 75 546 1236 1156
Cuts at: 5257 1790
Size: 2573
Fragments arranged by size:
2573 1236 1156 546 232 86 79 75
57
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6040
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2314 2442 3771
Size: 416 53 83 399 24 897 128 1329
Cuts at: 3771 4729 442
Size: 958 1753
Fragments arranged by size:
1753 1329 958 897 416 399 128 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6040
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2228 4700 1239
Size: 989 2472 2579
Fragments arranged by size:
2579 2472 989
Bpu10I CC'TnA_GC
Cuts at: 3324 3324
Size: 6040
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4165 5030 5328 4165
Size: 865 298 4877
Fragments arranged by size:
4877 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2591 4703 882
Size: 617 1092 2112 2219
Fragments arranged by size:
2219 2112 1092 617
BsaAI yAC'GTr
Cuts at: 493 1204 2182 3831 493
Size: 711 978 1649 2702
Fragments arranged by size:
2702 1649 978 711
BsaBI GATnn'nnATC
Cuts at: 6034 6034
Size: 6040
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2055 3847 4229 4864
Size: 53 83 186 936 522 1792 382 635
Cuts at: 4864 275
Size: 1451
Fragments arranged by size:
1792 1451 936 635 522 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4473 4871 5145 5292 5703 4473
Size: 398 274 147 411 4810
Fragments arranged by size:
4810 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3293 3323 1178
Size: 30 165 30 1890 30 3895
Fragments arranged by size:
3895 1890 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2444 2489 3311 3314 3329 3332 3341 3344
Size: 1258 45 822 3 15 3 9 3
Cuts at: 3344 1186
Size: 3882
Fragments arranged by size:
3882 1258 822 45 15 9 3 3
3
BseYI C'CCAG_C
Cuts at: 2294 2874 5243 5739 2294
Size: 580 2369 496 2595
Fragments arranged by size:
2595 2369 580 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2777 3173 1163
Size: 573 1041 396 4030
Fragments arranged by size:
4030 1041 573 396
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2264 3490 4251 5279 1540
Size: 275 186 263 1226 761 1028 2301
Fragments arranged by size:
2301 1226 1028 761 275 263 186
BsiHKAI G_wGCw'C
Cuts at: 2022 2088 2199 2871 4101 4186 5257 2022
Size: 66 111 672 1230 85 1071 2805
Fragments arranged by size:
2805 1230 1071 672 111 85 66
BsmI GAATG_Cn'
Cuts at: 3551 3644 3551
Size: 93 5947
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2088 2108 2187 2199 2214 2244
Size: 399 232 66 20 79 12 15 30
Cuts at: 2244 2319 2550 2865 2871 3359 4101 4186 5257
Size: 75 231 315 6 488 742 85 1071
Cuts at: 5257 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 488 399 315 232 231
85 79 75 66 30 20 15 12
6
BspEI T'CCGG_A
Cuts at: 5703 5703
Size: 6040
BspHI T'CATG_A
Cuts at: 2493 3929 5659 2493
Size: 1436 1730 2874
Fragments arranged by size:
2874 1730 1436
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2136 2726 2760 3395 844
Size: 1292 590 34 635 3489
Fragments arranged by size:
3489 1292 635 590 34
BsrBI CCG'CTC
Cuts at: 1394 1823 3927 1394
Size: 429 2104 3507
Fragments arranged by size:
3507 2104 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4535 4709 65
Size: 1183 3287 174 1396
Fragments arranged by size:
3287 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2168 4684 4871 1700
Size: 110 358 2516 187 2869
Fragments arranged by size:
2869 2516 358 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6040
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6040
BssSI C'ACGA_G
Cuts at: 2198 4100 5112 2198
Size: 1902 1012 3126
Fragments arranged by size:
3126 1902 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6040
BstBI TT'CG_AA
Cuts at: 4844 4844
Size: 6040
BstEII G'GTnAC_C
Cuts at: 1184 2672 2710 1184
Size: 1488 38 4514
Fragments arranged by size:
4514 1488 38
BstYI r'GATC_y
Cuts at: 1972 2203 2253 2466 3036 3370 3737 4122 4139
Size: 231 50 213 570 334 367 385 17
Cuts at: 4139 5591 5677 5689 6035 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 570 385 367 346 334 231
213 86 50 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2100 2113 2220 2923 513
Size: 552 1035 13 107 703 3630
Fragments arranged by size:
3630 1035 703 552 107 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2094 487
Size: 683 56 868 4433
Fragments arranged by size:
4433 868 683 56
BtsI GCAGTG_nn'
Cuts at: 2042 3556 4350 4370 2042
Size: 1514 794 20 3712
Fragments arranged by size:
3712 1514 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6040
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3088 3123 529
Size: 35 2524 35 3446
Fragments arranged by size:
3446 2524 35 35
DraI TTT'AAA
Cuts at: 3344 3344
Size: 6040
DraIII CAC_nnn'GTG
Cuts at: 2689 2689
Size: 6040
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5047 809
Size: 507 3731 1802
Fragments arranged by size:
3731 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2261 2926 3120
Size: 54 1425 50 275 18 431 665 194
Cuts at: 3120 3162 3336 3487 4376 6005 8
Size: 42 174 151 889 1629 43
Fragments arranged by size:
1629 1425 889 665 431 275 194 174
151 54 50 43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 2261 3487 1537
Size: 275 449 1226 4090
Fragments arranged by size:
4090 1226 449 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3970 1353
Size: 2617 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3085 4627 5001 5147 387
Size: 1064 138 1496 1542 374 146 1280
Fragments arranged by size:
1542 1496 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2869 2869
Size: 6040
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2228 2623 4103 4700 5487 5755 1239
Size: 393 596 395 1480 597 787 268 1524
Fragments arranged by size:
1524 1480 787 597 596 395 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2240 2241 2240
Size: 1 6039
EcoRI G'AATT_C
Cuts at: 3349 3727 3349
Size: 378 5662
FspI TGC'GCA
Cuts at: 4546 4546
Size: 6040
HaeII r_GCGC'y
Cuts at: 1678 2058 2855 5187 1678
Size: 380 797 2332 2531
Fragments arranged by size:
2531 2332 797 380
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3245 3277 4681 4713 4755
Size: 32 135 32 1673 32 1404 32 42
Cuts at: 4755 4787 1373
Size: 32 2626
Fragments arranged by size:
2626 1673 1404 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3384 3630 669
Size: 988 281 1446 246 3079
Fragments arranged by size:
3079 1446 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6040
HpaI GTT'AAC
Cuts at: 3630 3630
Size: 6040
KasI G'GCGC_C
Cuts at: 2054 2054
Size: 6040
KpnI G_GTAC'C
Cuts at: 2451 3365 2451
Size: 914 5126
MfeI C'AATT_G
Cuts at: 3639 3639
Size: 6040
MluI A'CGCG_T
Cuts at: 5916 5916
Size: 6040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5154 5338 1305
Size: 3849 184 2007
Fragments arranged by size:
3849 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4218
Fragments arranged by size:
4218 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2147 2540 2969 3164 3998 4357 4516
Size: 648 981 393 429 195 834 359 159
Cuts at: 4516 518
Size: 2042
Fragments arranged by size:
2042 981 834 648 429 393 359 195
159
MspA1I CmG'CkG
Cuts at: 1606 2134 2407 3456 4133 5281 5526 1606
Size: 528 273 1049 677 1148 245 2120
Fragments arranged by size:
2120 1148 1049 677 528 273 245
NarI GG'CG_CC
Cuts at: 2055 2055
Size: 6040
NcoI C'CATG_G
Cuts at: 513 1065 2220 2923 513
Size: 552 1155 703 3630
Fragments arranged by size:
3630 1155 703 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6040
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6040
NotI GC'GGCC_GC
Cuts at: 3487 3487
Size: 6040
NspI r_CATG'y
Cuts at: 1132 1229 2968 3398 4943 1132
Size: 97 1739 430 1545 2229
Fragments arranged by size:
2229 1739 1545 430 97
PasI CC'CwG_GG
Cuts at: 1348 2419 1348
Size: 1071 4969
PciI A'CATG_T
Cuts at: 4939 4939
Size: 6040
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2064 2419 1108
Size: 956 355 4729
Fragments arranged by size:
4729 956 355
PfoI T'CCnGG_A
Cuts at: 2469 3126 2469
Size: 657 5383
PmeI GTTT'AAAC
Cuts at: 3344 3344
Size: 6040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3209 3241 3293 3325 4109 4141 5622 5654 3209
Size: 32 52 32 784 32 1481 32 3595
Fragments arranged by size:
3595 1481 784 52 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6040
PsiI TTA'TAA
Cuts at: 3610 3610
Size: 6040
PspOMI G'GGCC_C
Cuts at: 2240 2240
Size: 6040
PstI C_TGCA'G
Cuts at: 830 2133 2397 3392 830
Size: 1303 264 995 3478
Fragments arranged by size:
3478 1303 995 264
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6040
PvuII CAG'CTG
Cuts at: 2134 2407 2134
Size: 273 5767
SacI G_AGCT'C
Cuts at: 2871 2871
Size: 6040
SalI G'TCGA_C
Cuts at: 1936 3382 1936
Size: 1446 4594
SbfI CC_TGCA'GG
Cuts at: 2397 3392 2397
Size: 995 5045
ScaI AGT'ACT
Cuts at: 4288 6000 4288
Size: 1712 4328
SexAI A'CCwGG_T
Cuts at: 2685 3255 2685
Size: 570 5470
SfcI C'TryA_G
Cuts at: 826 1045 2129 2393 3388 4523 5204 5395 826
Size: 219 1084 264 995 1135 681 191 1471
Fragments arranged by size:
1471 1135 1084 995 681 264 219 191
SfoI GGC'GCC
Cuts at: 2056 2056
Size: 6040
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6040
SgrAI Cr'CCGG_yG
Cuts at: 1700 2168 1700
Size: 468 5572
SmaI CCC'GGG
Cuts at: 1872 3367 1872
Size: 1495 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4144 5045 5307 820
Size: 197 926 2201 901 262 1553
Fragments arranged by size:
2201 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3831 493
Size: 3338 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6040
SphI G_CATG'C
Cuts at: 1132 3398 1132
Size: 2266 3774
SspI AAT'ATT
Cuts at: 5 52 3964 5
Size: 47 3912 2081
Fragments arranged by size:
3912 2081 47
StuI AGG'CCT
Cuts at: 1517 3182 1517
Size: 1665 4375
StyI C'CwwG_G
Cuts at: 513 1065 2220 2310 2923 3479 513
Size: 552 1155 90 613 556 3074
Fragments arranged by size:
3074 1155 613 556 552 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2270 2411 2888 4067 4084 4237 4422
Size: 1255 690 141 477 1179 17 153 185
Cuts at: 4422 4867 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1179 690 477 445 185 153
141 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2151 4286 5998
Size: 274 80 33 51 1443 174 2135 1712
Cuts at: 5998 96
Size: 138
Fragments arranged by size:
2135 1712 1443 274 174 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6000 6032 6000
Size: 32 6008
Tth111I GACn'n_nGTC
Cuts at: 1316 2434 1316
Size: 1118 4922
XbaI T'CTAG_A
Cuts at: 3376 3376
Size: 6040
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2420 2899 2977 2420
Size: 479 78 5483
Fragments arranged by size:
5483 479 78
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6040
XmaI C'CCGG_G
Cuts at: 1870 3365 1870
Size: 1495 4545
XmnI GAAnn'nnTTC
Cuts at: 4169 4169
Size: 6040
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3848 276
Size: 53 83 186 936 2314 2468
Fragments arranged by size:
2468 2314 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstXI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI
NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI
PsrI RsrII SacII SanDI SapI SfiI SrfI SwaI