Restriction map for
pFN21AE1907
(Circular) (Six-base) MAPSORT of: pFN21AE1907.seq Check: 8851 from: 1 to: 6031
pFN21AE1907 6031 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3841 278
Size: 53 83 186 936 2305 2468
Fragments arranged by size:
2468 2305 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2151 3374 1937
Size: 214 1223 4594
Fragments arranged by size:
4594 1223 214
Acc65I G'GTAC_C
Cuts at: 3352 3352
Size: 6031
AclI AA'CG_TT
Cuts at: 1563 4158 4531 1563
Size: 2595 373 3063
Fragments arranged by size:
3063 2595 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4094 5478 5746 1632
Size: 2462 1384 268 1917
Fragments arranged by size:
2462 1917 1384 268
AfeI AGC'GCT
Cuts at: 2501 2501
Size: 6031
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5834
AflIII A'CryG_T
Cuts at: 1203 2415 2629 3171 4930 5907 1203
Size: 1212 214 542 1759 977 1327
Fragments arranged by size:
1759 1327 1212 977 542 214
AgeI A'CCGG_T
Cuts at: 4862 4862
Size: 6031
AhdI GACnn_n'nnGTC
Cuts at: 4760 4760
Size: 6031
AleI CACnn'nnGTG
Cuts at: 1166 2099 1166
Size: 933 5098
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6031
AlwNI CAG_nnn'CTG
Cuts at: 3774 5346 3774
Size: 1572 4459
ApaI G_GGCC'C
Cuts at: 2044 2382 2683 2044
Size: 338 301 5392
Fragments arranged by size:
5392 338 301
ApaLI G'TGCA_C
Cuts at: 4088 5244 4088
Size: 1156 4875
ApoI r'AATT_y
Cuts at: 1493 3340 3566 3718 5827 1493
Size: 1847 226 152 2109 1697
Fragments arranged by size:
2109 1847 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4585 160
Size: 872 3553 1606
Fragments arranged by size:
3553 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6031
AvaI C'yCGr_G
Cuts at: 1870 1943 3356 1870
Size: 73 1413 4545
Fragments arranged by size:
4545 1413 73
BamHI G'GATC_C
Cuts at: 3361 3728 3361
Size: 367 5664
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2812 3291 3352 4807
Size: 325 217 627 221 804 479 61 1455
Cuts at: 4807 618
Size: 1842
Fragments arranged by size:
1842 1455 804 627 479 325 221 217
61
BanII G_rGCy'C
Cuts at: 1391 2044 2072 2127 2382 2683 3005 3350 1391
Size: 653 28 55 255 301 322 345 4072
Fragments arranged by size:
4072 653 345 322 301 255 55 28
BbeI G_GCGC'C
Cuts at: 2012 2012
Size: 6031
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5060
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3221 3255 4222 4256 1903
Size: 34 1284 34 967 34 3678
Fragments arranged by size:
3678 1284 967 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3923 5133 3923
Size: 1210 4821
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6031
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3224 4642 136
Size: 107 122 71 631 605 1552 1418 1525
Fragments arranged by size:
1552 1525 1418 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6026 6026
Size: 6031
BlpI GC'TnA_GC
Cuts at: 3448 3448
Size: 6031
Bme1580I G_kGCm'C
Cuts at: 1790 2044 2382 2683 4092 5248 1790
Size: 254 338 301 1409 1156 2573
Fragments arranged by size:
2573 1409 1156 338 301 254
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6031
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3762 4720 442
Size: 416 53 83 399 24 2345 958 1753
Fragments arranged by size:
2345 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6031
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3045 3045
Size: 6031
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2336 2567 2582 2841 2985 4691 1239
Size: 1097 231 15 259 144 1706 2579
Fragments arranged by size:
2579 1706 1097 259 231 144 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2101 4156 5021 5319 2101
Size: 2055 865 298 2813
Fragments arranged by size:
2813 2055 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2346 2756 4694 882
Size: 617 847 410 1938 2219
Fragments arranged by size:
2219 1938 847 617 410
BsaAI yAC'GTr
Cuts at: 493 1204 2149 3822 493
Size: 711 945 1673 2702
Fragments arranged by size:
2702 1673 945 711
BsaBI GATnn'nnATC
Cuts at: 6025 6025
Size: 6031
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2184 2389 3838
Size: 53 83 186 936 476 175 205 1449
Cuts at: 3838 4220 4855 275
Size: 382 635 1451
Fragments arranged by size:
1451 1449 936 635 476 382 205 186
175 83 53
BsaWI w'CCGG_w
Cuts at: 3132 4464 4862 5136 5283 5694 3132
Size: 1332 398 274 147 411 3469
Fragments arranged by size:
3469 1332 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2379 2409 2444 2474 2540
Size: 30 165 30 976 30 35 30 66
Cuts at: 2540 2570 2943 2958 2973 2988 3127 3157 1178
Size: 30 373 15 15 15 139 30 4052
Fragments arranged by size:
4052 976 373 165 139 66 35 30
30 30 30 30 30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2012 2384 2543 1186
Size: 826 372 159 4674
Fragments arranged by size:
4674 826 372 159
BseYI C'CCAG_C
Cuts at: 2336 2496 2676 2868 2901 5234 5730 2336
Size: 160 180 192 33 2333 496 2637
Fragments arranged by size:
2637 2333 496 192 180 160 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2870 1163
Size: 573 1134 4324
Fragments arranged by size:
4324 1134 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2116 3481 4242 5270 1540
Size: 275 186 115 1365 761 1028 2301
Fragments arranged by size:
2301 1365 1028 761 275 186 115
BsiHKAI G_wGCw'C
Cuts at: 3005 3108 4092 4177 5248 3005
Size: 103 984 85 1071 3788
Fragments arranged by size:
3788 1071 984 103 85
BsiWI C'GTAC_G
Cuts at: 2418 2418
Size: 6031
BsmI GAATG_Cn'
Cuts at: 3542 3635 3542
Size: 93 5938
BsmBI CGTCTCn'nnnn_
Cuts at: 2591 2591
Size: 6031
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2044 2072 2127 2382 2683 3005 3108
Size: 399 254 28 55 255 301 322 103
Cuts at: 3108 3350 4092 4177 5248 1391
Size: 242 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 322 301 255 254
242 103 85 55 28
BspEI T'CCGG_A
Cuts at: 3132 5694 3132
Size: 2562 3469
BspHI T'CATG_A
Cuts at: 3920 5650 3920
Size: 1730 4301
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2316 2463 3386 844
Size: 1472 147 923 3489
Fragments arranged by size:
3489 1472 923 147
BsrBI CCG'CTC
Cuts at: 1394 1823 3918 1394
Size: 429 2095 3507
Fragments arranged by size:
3507 2095 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4526 4700 65
Size: 1183 3278 174 1396
Fragments arranged by size:
3278 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4675 4862 1700
Size: 110 2865 187 2869
Fragments arranged by size:
2869 2865 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6031
BssHII G'CGCG_C
Cuts at: 1925 2608 1925
Size: 683 5348
BssSI C'ACGA_G
Cuts at: 2855 4091 5103 2855
Size: 1236 1012 3783
Fragments arranged by size:
3783 1236 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2167 2633 794
Size: 1373 466 4192
Fragments arranged by size:
4192 1373 466
BstBI TT'CG_AA
Cuts at: 4835 4835
Size: 6031
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6031
BstYI r'GATC_y
Cuts at: 1972 2614 3177 3361 3728 4113 4130 5582 5668
Size: 642 563 184 367 385 17 1452 86
Cuts at: 5668 5680 6026 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 642 563 385 367 346 184
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2300 2669 2300
Size: 369 5662
BtgI C'CryG_G
Cuts at: 513 1065 2004 2116 2139 2207 2216 2398 2469
Size: 552 939 112 23 68 9 182 71
Cuts at: 2469 2481 513
Size: 12 4063
Fragments arranged by size:
4063 939 552 182 112 71 68 23
12 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2119 487
Size: 683 56 893 4399
Fragments arranged by size:
4399 893 683 56
BtsI GCAGTG_nn'
Cuts at: 3213 3547 4341 4361 3213
Size: 334 794 20 4883
Fragments arranged by size:
4883 794 334 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6031
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5996
DraI TTT'AAA
Cuts at: 3335 3335
Size: 6031
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5038 809
Size: 507 3722 1802
Fragments arranged by size:
3722 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2113 3478 4367
Size: 54 1425 50 275 18 283 1365 889
Cuts at: 4367 5996 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1365 889 283 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2113 3478 1537
Size: 275 301 1365 4090
Fragments arranged by size:
4090 1365 301 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2993 3113 3961 1353
Size: 1640 120 848 3423
Fragments arranged by size:
3423 1640 848 120
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2303 2489 4618 4992 5138 387
Size: 1064 138 714 186 2129 374 146 1280
Fragments arranged by size:
2129 1280 1064 714 374 186 146 138
EcoICRI GAG'CTC
Cuts at: 3003 3003
Size: 6031
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2336 2567 2582 2841 2985 4094 4691
Size: 393 704 231 15 259 144 1109 597
Cuts at: 4691 5478 5746 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1109 787 704 597 393 268 259
231 144 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2447 2447
Size: 6031
EcoO109I rG'GnC_Cy
Cuts at: 2040 2041 2378 2452 2538 2680 2040
Size: 1 337 74 86 142 5391
Fragments arranged by size:
5391 337 142 86 74 1
EcoRI G'AATT_C
Cuts at: 3340 3718 3340
Size: 378 5653
FspI TGC'GCA
Cuts at: 4537 4537
Size: 6031
HaeII r_GCGC'y
Cuts at: 1678 2012 2503 5178 1678
Size: 334 491 2675 2531
Fragments arranged by size:
2675 2531 491 334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2324 2356 2379 2411 2423
Size: 32 135 32 752 32 23 32 12
Cuts at: 2423 2444 2455 2476 2943 2958 2975 2990 4672
Size: 21 11 21 467 15 17 15 1682
Cuts at: 4672 4704 4746 4778 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1682 752 467 135 42 32 32
32 32 32 32 23 21 21 17
15 15 12 11
HincII GTy'rAC
Cuts at: 669 1657 1938 3375 3621 669
Size: 988 281 1437 246 3079
Fragments arranged by size:
3079 1437 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3085 748
Size: 2337 3694
HpaI GTT'AAC
Cuts at: 3621 3621
Size: 6031
KasI G'GCGC_C
Cuts at: 2008 2008
Size: 6031
KpnI G_GTAC'C
Cuts at: 3356 3356
Size: 6031
MfeI C'AATT_G
Cuts at: 3630 3630
Size: 6031
MluI A'CGCG_T
Cuts at: 2415 5907 2415
Size: 3492 2539
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5145 5329 1305
Size: 3840 184 2007
Fragments arranged by size:
3840 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4209
Fragments arranged by size:
4209 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2099 2138 3989 4348 4507 518
Size: 648 933 39 1851 359 159 2042
Fragments arranged by size:
2042 1851 933 648 359 159 39
MspA1I CmG'CkG
Cuts at: 1606 2099 2118 2209 2400 2471 2722 2804 3057
Size: 493 19 91 191 71 251 82 253
Cuts at: 3057 3447 4124 5272 5517 1606
Size: 390 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 493 390 253 251 245
191 91 82 71 19
NarI GG'CG_CC
Cuts at: 2009 2009
Size: 6031
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4540
Fragments arranged by size:
4540 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6031
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6031
NotI GC'GGCC_GC
Cuts at: 3478 3478
Size: 6031
NspI r_CATG'y
Cuts at: 1132 1229 2844 3175 3389 4934 1132
Size: 97 1615 331 214 1545 2229
Fragments arranged by size:
2229 1615 1545 331 214 97
PasI CC'CwG_GG
Cuts at: 1348 2683 1348
Size: 1335 4696
PciI A'CATG_T
Cuts at: 3171 4930 3171
Size: 1759 4272
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6031
PfoI T'CCnGG_A
Cuts at: 2462 2462
Size: 6031
PmeI GTTT'AAAC
Cuts at: 3335 3335
Size: 6031
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4100 4132 5613 5645 4100
Size: 32 1481 32 4486
Fragments arranged by size:
4486 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2452 2538 2452
Size: 86 5945
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6031
PsiI TTA'TAA
Cuts at: 3601 3601
Size: 6031
PspOMI G'GGCC_C
Cuts at: 2040 2378 2679 2040
Size: 338 301 5392
Fragments arranged by size:
5392 338 301
PstI C_TGCA'G
Cuts at: 830 2776 3040 3383 830
Size: 1946 264 343 3478
Fragments arranged by size:
3478 1946 343 264
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6031
SacI G_AGCT'C
Cuts at: 3005 3005
Size: 6031
SacII CC_GC'GG
Cuts at: 2119 2210 2401 2472 2119
Size: 91 191 71 5678
Fragments arranged by size:
5678 191 91 71
SalI G'TCGA_C
Cuts at: 1936 3373 1936
Size: 1437 4594
SapI GCTCTTCn'nnn_
Cuts at: 2993 3113 2993
Size: 120 5911
SbfI CC_TGCA'GG
Cuts at: 2776 3383 2776
Size: 607 5424
ScaI AGT'ACT
Cuts at: 4279 5991 4279
Size: 1712 4319
SfcI C'TryA_G
Cuts at: 826 1045 2603 2772 3036 3379 4514 5195 5386
Size: 219 1558 169 264 343 1135 681 191
Cuts at: 5386 826
Size: 1471
Fragments arranged by size:
1558 1471 1135 681 343 264 219 191
169
SfoI GGC'GCC
Cuts at: 2010 2010
Size: 6031
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6031
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6031
SmaI CCC'GGG
Cuts at: 1872 3358 1872
Size: 1486 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2080 4135 5036 5298 820
Size: 197 926 137 2055 901 262 1553
Fragments arranged by size:
2055 1553 926 901 262 197 137
SnaBI TAC'GTA
Cuts at: 493 2149 3822 493
Size: 1656 1673 2702
Fragments arranged by size:
2702 1673 1656
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6031
SphI G_CATG'C
Cuts at: 1132 3389 1132
Size: 2257 3774
SspI AAT'ATT
Cuts at: 5 52 3955 5
Size: 47 3903 2081
Fragments arranged by size:
3903 2081 47
StuI AGG'CCT
Cuts at: 1517 2770 1517
Size: 1253 4778
StyI C'CwwG_G
Cuts at: 513 1065 2004 2268 2947 3112 3470 513
Size: 552 939 264 679 165 358 3074
Fragments arranged by size:
3074 939 679 552 358 264 165
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2069 2209 2304 2510 3155 4058 4075
Size: 1255 489 140 95 206 645 903 17
Cuts at: 4075 4228 4413 4858 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 903 645 489 445 206 185
153 140 95 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4277 5989 96
Size: 274 80 33 51 1443 2300 1712 138
Fragments arranged by size:
2300 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2540 2572 3127 3151 3159 3183 5991 6023 2540
Size: 32 555 24 8 24 2808 32 2548
Fragments arranged by size:
2808 2548 555 32 32 24 24 8
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6031
XbaI T'CTAG_A
Cuts at: 3367 3367
Size: 6031
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2333 2360 2917 2333
Size: 27 557 5447
Fragments arranged by size:
5447 557 27
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6031
XmaI C'CCGG_G
Cuts at: 1870 3356 1870
Size: 1486 4545
XmnI GAAnn'nnTTC
Cuts at: 4160 4160
Size: 6031
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3839 276
Size: 53 83 186 936 2305 2468
Fragments arranged by size:
2468 2305 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BstXI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII
RsrII SanDI SexAI SfiI SrfI SwaI