Restriction map for
pFN21AE1903
(Circular) (Six-base) MAPSORT of: pFN21AE1903.seq Check: 3456 from: 1 to: 6004
pFN21AE1903 6004 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3814 278
Size: 53 83 186 936 2278 2468
Fragments arranged by size:
2468 2278 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3347 1937
Size: 1410 4594
Acc65I G'GTAC_C
Cuts at: 3325 3325
Size: 6004
AclI AA'CG_TT
Cuts at: 1563 4131 4504 1563
Size: 2568 373 3063
Fragments arranged by size:
3063 2568 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3239 4067 5451 5719 1632
Size: 1607 828 1384 268 1917
Fragments arranged by size:
1917 1607 1384 828 268
AfeI AGC'GCT
Cuts at: 2036 2036
Size: 6004
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5807
AflIII A'CryG_T
Cuts at: 1203 3012 3018 4903 5880 1203
Size: 1809 6 1885 977 1327
Fragments arranged by size:
1885 1809 1327 977 6
AgeI A'CCGG_T
Cuts at: 2760 4835 2760
Size: 2075 3929
AhdI GACnn_n'nnGTC
Cuts at: 4733 4733
Size: 6004
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6004
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2239 1184
Size: 1055 4949
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2087 2119 2087
Size: 32 5972
AlwNI CAG_nnn'CTG
Cuts at: 3747 5319 3747
Size: 1572 4432
ApaLI G'TGCA_C
Cuts at: 4061 5217 4061
Size: 1156 4848
ApoI r'AATT_y
Cuts at: 1493 2498 3313 3539 3691 5800 1493
Size: 1005 815 226 152 2109 1697
Fragments arranged by size:
2109 1697 1005 815 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4558 160
Size: 872 3526 1606
Fragments arranged by size:
3526 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6004
AvaI C'yCGr_G
Cuts at: 1870 1943 2047 3329 1870
Size: 73 104 1282 4545
Fragments arranged by size:
4545 1282 104 73
BamHI G'GATC_C
Cuts at: 3334 3701 3334
Size: 367 5637
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3325 4780 618
Size: 325 217 627 1538 1455 1842
Fragments arranged by size:
1842 1538 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2074 2973 2993 3082 3323 1391
Size: 683 899 20 89 241 4072
Fragments arranged by size:
4072 899 683 241 89 20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5033
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4195 4229 1903
Size: 34 2258 34 3678
Fragments arranged by size:
3678 2258 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3896 5106 3896
Size: 1210 4794
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6004
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2025 4615
Size: 107 122 71 631 605 337 16 2590
Cuts at: 4615 136
Size: 1525
Fragments arranged by size:
2590 1525 631 605 337 122 107 71
16
BglII A'GATC_T
Cuts at: 2961 5999 2961
Size: 3038 2966
BlpI GC'TnA_GC
Cuts at: 2849 2972 3421 2849
Size: 123 449 5432
Fragments arranged by size:
5432 449 123
Bme1580I G_kGCm'C
Cuts at: 1790 4065 5221 1790
Size: 2275 1156 2573
Fragments arranged by size:
2573 2275 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6004
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3079 3735 4693
Size: 416 53 83 399 24 1662 656 958
Cuts at: 4693 442
Size: 1753
Fragments arranged by size:
1753 1662 958 656 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6004
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2051 2978 4664 1239
Size: 812 927 1686 2579
Fragments arranged by size:
2579 1686 927 812
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4129 4994 5292 4129
Size: 865 298 4841
Fragments arranged by size:
4841 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4667 882
Size: 617 3168 2219
Fragments arranged by size:
3168 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2128 3795 493
Size: 711 924 1667 2702
Fragments arranged by size:
2702 1667 924 711
BsaBI GATnn'nnATC
Cuts at: 5998 5998
Size: 6004
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3811 4193 4828 275
Size: 53 83 186 936 2278 382 635 1451
Fragments arranged by size:
2278 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2760 2841 2919 4437 4835 5109 5256 5667 2760
Size: 81 78 1518 398 274 147 411 3097
Fragments arranged by size:
3097 1518 411 398 274 147 81 78
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2468 2498 2694 2724 3134
Size: 30 165 30 1065 30 196 30 410
Cuts at: 3134 3164 1178
Size: 30 4018
Fragments arranged by size:
4018 1065 410 196 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2720 1186
Size: 1534 4470
BseYI C'CCAG_C
Cuts at: 5207 5703 5207
Size: 496 5508
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5431
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3454 4215 5243 1540
Size: 275 186 1453 761 1028 2301
Fragments arranged by size:
2301 1453 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2133 2973 2993 4065 4150 5221 2133
Size: 840 20 1072 85 1071 2916
Fragments arranged by size:
2916 1072 1071 840 85 20
BsmI GAATG_Cn'
Cuts at: 3515 3608 3515
Size: 93 5911
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2074 2133 2973 2993 3082 3323 4065
Size: 399 284 59 840 20 89 241 742
Cuts at: 4065 4150 5221 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 840 742 399 284 241 89
85 59 20
BspEI T'CCGG_A
Cuts at: 2841 2919 5667 2841
Size: 78 2748 3178
Fragments arranged by size:
3178 2748 78
BspHI T'CATG_A
Cuts at: 3893 5623 3893
Size: 1730 4274
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3359 844
Size: 2515 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2146 3891 1394
Size: 429 323 1745 3507
Fragments arranged by size:
3507 1745 429 323
BsrDI GCAATG_nn'
Cuts at: 65 1248 2233 3228 4499 4673 65
Size: 1183 985 995 1271 174 1396
Fragments arranged by size:
1396 1271 1183 995 985 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2760 4648 4835 1700
Size: 110 950 1888 187 2869
Fragments arranged by size:
2869 1888 950 187 110
BsrGI T'GTAC_A
Cuts at: 96 2683 3015 3057 96
Size: 2587 332 42 3043
Fragments arranged by size:
3043 2587 332 42
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6004
BssSI C'ACGA_G
Cuts at: 4064 5076 4064
Size: 1012 4992
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2157 794
Size: 1363 4641
BstBI TT'CG_AA
Cuts at: 4808 4808
Size: 6004
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6004
BstYI r'GATC_y
Cuts at: 1972 2961 3334 3701 4086 4103 5555 5641 5653
Size: 989 373 367 385 17 1452 86 12
Cuts at: 5653 5999 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 989 385 373 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2280 3042 513
Size: 552 1215 762 3475
Fragments arranged by size:
3475 1215 762 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5265
Fragments arranged by size:
5265 683 56
BtsI GCAGTG_nn'
Cuts at: 2155 3520 4314 4334 2155
Size: 1365 794 20 3825
Fragments arranged by size:
3825 1365 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6004
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5969
DraI TTT'AAA
Cuts at: 3308 3308
Size: 6004
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5011 809
Size: 507 3695 1802
Fragments arranged by size:
3695 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2343 3451 4340
Size: 54 1425 50 275 18 513 1108 889
Cuts at: 4340 5969 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1108 889 513 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3451 1537
Size: 275 1639 4090
Fragments arranged by size:
4090 1639 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2907 3934 1353
Size: 1554 1027 3423
Fragments arranged by size:
3423 1554 1027
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4591 4965 5111 387
Size: 1064 138 3002 374 146 1280
Fragments arranged by size:
3002 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2971 2991 2971
Size: 20 5984
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2978 3239 4067 4664 5451 5719
Size: 393 419 927 261 828 597 787 268
Cuts at: 5719 1239
Size: 1524
Fragments arranged by size:
1524 927 828 787 597 419 393 268
261
EcoNI CCTnn'n_nnAGG
Cuts at: 3221 3221
Size: 6004
EcoO109I rG'GnC_Cy
Cuts at: 2017 2661 2017
Size: 644 5360
EcoRI G'AATT_C
Cuts at: 2498 3313 3691 2498
Size: 815 378 4811
Fragments arranged by size:
4811 815 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2790 2790
Size: 6004
FspI TGC'GCA
Cuts at: 4510 4510
Size: 6004
HaeII r_GCGC'y
Cuts at: 1678 2038 5151 1678
Size: 360 3113 2531
Fragments arranged by size:
3113 2531 360
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2393 2425 2871 2903 3084
Size: 32 135 32 821 32 446 32 181
Cuts at: 3084 3116 4645 4677 4719 4751 1373
Size: 32 1529 32 42 32 2626
Fragments arranged by size:
2626 1529 821 446 181 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2140 2542 3348 3594 669
Size: 988 281 202 402 806 246 3079
Fragments arranged by size:
3079 988 806 402 281 246 202
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6004
HpaI GTT'AAC
Cuts at: 2542 3594 2542
Size: 1052 4952
KpnI G_GTAC'C
Cuts at: 3329 3329
Size: 6004
MfeI C'AATT_G
Cuts at: 2559 3603 2559
Size: 1044 4960
MluI A'CGCG_T
Cuts at: 5880 5880
Size: 6004
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5118 5302 1305
Size: 3813 184 2007
Fragments arranged by size:
3813 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2345 10
Size: 54 1425 343 513 3669
Fragments arranged by size:
3669 1425 513 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3962 4321 4480 518
Size: 648 2796 359 159 2042
Fragments arranged by size:
2796 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3420 4097 5245 5490 1606
Size: 1814 677 1148 245 2120
Fragments arranged by size:
2120 1814 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2280 3042 513
Size: 552 1215 762 3475
Fragments arranged by size:
3475 1215 762 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6004
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6004
NotI GC'GGCC_GC
Cuts at: 3451 3451
Size: 6004
NspI r_CATG'y
Cuts at: 1132 1229 3022 3064 3362 4907 1132
Size: 97 1793 42 298 1545 2229
Fragments arranged by size:
2229 1793 1545 298 97 42
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6004
PciI A'CATG_T
Cuts at: 3018 4903 3018
Size: 1885 4119
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3043 1108
Size: 1935 4069
PmeI GTTT'AAAC
Cuts at: 3308 3308
Size: 6004
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4073 4105 5586 5618 4073
Size: 32 1481 32 4459
Fragments arranged by size:
4459 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2661 2661
Size: 6004
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6004
PsiI TTA'TAA
Cuts at: 3574 3574
Size: 6004
PstI C_TGCA'G
Cuts at: 830 2045 2079 3356 830
Size: 1215 34 1277 3478
Fragments arranged by size:
3478 1277 1215 34
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6004
SacI G_AGCT'C
Cuts at: 2973 2993 2973
Size: 20 5984
SalI G'TCGA_C
Cuts at: 1936 3346 1936
Size: 1410 4594
SanDI GG'GwC_CC
Cuts at: 2661 2661
Size: 6004
SbfI CC_TGCA'GG
Cuts at: 3356 3356
Size: 6004
ScaI AGT'ACT
Cuts at: 2165 2526 4252 5964 2165
Size: 361 1726 1712 2205
Fragments arranged by size:
2205 1726 1712 361
SfcI C'TryA_G
Cuts at: 826 1045 2041 2075 3112 3352 4487 5168 5359
Size: 219 996 34 1037 240 1135 681 191
Cuts at: 5359 826
Size: 1471
Fragments arranged by size:
1471 1135 1037 996 681 240 219 191
34
SfiI GGCCn_nnn'nGGCC
Cuts at: 2025 2025
Size: 6004
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6004
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6004
SmaI CCC'GGG
Cuts at: 1872 2049 3331 1872
Size: 177 1282 4545
Fragments arranged by size:
4545 1282 177
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4108 5009 5271 820
Size: 197 926 2165 901 262 1553
Fragments arranged by size:
2165 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3795 493
Size: 3302 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6004
SphI G_CATG'C
Cuts at: 1132 3362 1132
Size: 2230 3774
SrfI GCCC'GGGC
Cuts at: 2049 2049
Size: 6004
SspI AAT'ATT
Cuts at: 5 52 3928 5
Size: 47 3876 2081
Fragments arranged by size:
3876 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6004
StyI C'CwwG_G
Cuts at: 513 1065 2280 2811 2829 3042 3443 513
Size: 552 1215 531 18 213 401 3074
Fragments arranged by size:
3074 1215 552 531 401 213 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2376 3035 4031 4048 4201 4386 4831
Size: 1255 796 659 996 17 153 185 445
Cuts at: 4831 325
Size: 1498
Fragments arranged by size:
1498 1255 996 796 659 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2163 2524 2683
Size: 274 80 33 51 1443 186 361 159
Cuts at: 2683 3015 3057 3132 4250 5962 96
Size: 332 42 75 1118 1712 138
Fragments arranged by size:
1712 1443 1118 361 332 274 186 159
138 80 75 51 42 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2468 2500 2924 2956 5964 5996 2468
Size: 32 424 32 3008 32 2476
Fragments arranged by size:
3008 2476 424 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2885 1316
Size: 1569 4435
XbaI T'CTAG_A
Cuts at: 3340 3340
Size: 6004
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2263 2881 3235 2263
Size: 618 354 5032
Fragments arranged by size:
5032 618 354
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6004
XmaI C'CCGG_G
Cuts at: 1870 2047 3329 1870
Size: 177 1282 4545
Fragments arranged by size:
4545 1282 177
XmnI GAAnn'nnTTC
Cuts at: 2374 4133 2374
Size: 1759 4245
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3812 276
Size: 53 83 186 936 2278 2468
Fragments arranged by size:
2468 2278 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SacI SalI SanDI SbfI ScaI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII
RsrII SacII SapI SexAI SfoI SwaI