Restriction map for
pFN21AE1902
(Circular) (Six-base) MAPSORT of: pFN21AE1902.seq Check: 3114 from: 1 to: 5992
pFN21AE1902 5992 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2430 3802 278
Size: 53 83 186 936 894 1372 2468
Fragments arranged by size:
2468 1372 936 894 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3335 1937
Size: 1398 4594
Acc65I G'GTAC_C
Cuts at: 3313 3313
Size: 5992
AclI AA'CG_TT
Cuts at: 1563 4119 4492 1563
Size: 2556 373 3063
Fragments arranged by size:
3063 2556 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3197 3223 4055 5439 5707 1632
Size: 1565 26 832 1384 268 1917
Fragments arranged by size:
1917 1565 1384 832 268 26
AfeI AGC'GCT
Cuts at: 2690 2690
Size: 5992
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5795
AflIII A'CryG_T
Cuts at: 1203 2493 4891 5868 1203
Size: 1290 2398 977 1327
Fragments arranged by size:
2398 1327 1290 977
AgeI A'CCGG_T
Cuts at: 4823 4823
Size: 5992
AhdI GACnn_n'nnGTC
Cuts at: 2020 2935 4721 2020
Size: 915 1786 3291
Fragments arranged by size:
3291 1786 915
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5992
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5992
AlwNI CAG_nnn'CTG
Cuts at: 2095 2335 2338 2506 2848 2899 3735 5307 2095
Size: 240 3 168 342 51 836 1572 2780
Fragments arranged by size:
2780 1572 836 342 240 168 51 3
ApaLI G'TGCA_C
Cuts at: 4049 5205 4049
Size: 1156 4836
ApoI r'AATT_y
Cuts at: 1493 3301 3527 3679 5788 1493
Size: 1808 226 152 2109 1697
Fragments arranged by size:
2109 1808 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4546 160
Size: 872 3514 1606
Fragments arranged by size:
3514 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5992
AvaI C'yCGr_G
Cuts at: 1870 1943 2010 2017 2412 2765 2868 3036 3317
Size: 73 67 7 395 353 103 168 281
Cuts at: 3317 1870
Size: 4545
Fragments arranged by size:
4545 395 353 281 168 103 73 67
7
BamHI G'GATC_C
Cuts at: 3322 3689 3322
Size: 367 5625
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2255 3115 3313 4768 618
Size: 325 217 627 468 860 198 1455 1842
Fragments arranged by size:
1842 1455 860 627 468 325 217 198
BanII G_rGCy'C
Cuts at: 1391 2727 2766 2965 3264 3311 1391
Size: 1336 39 199 299 47 4072
Fragments arranged by size:
4072 1336 299 199 47 39
BbeI G_GCGC'C
Cuts at: 2259 2259
Size: 5992
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2673 928
Size: 971 774 4247
Fragments arranged by size:
4247 971 774
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4183 4217 1903
Size: 34 2246 34 3678
Fragments arranged by size:
3678 2246 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3884 5094 3884
Size: 1210 4782
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5992
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2159 2264 4603
Size: 107 122 71 631 605 487 105 2339
Cuts at: 4603 136
Size: 1525
Fragments arranged by size:
2339 1525 631 605 487 122 107 105
71
BglII A'GATC_T
Cuts at: 2784 5987 2784
Size: 3203 2789
BlpI GC'TnA_GC
Cuts at: 2339 3409 2339
Size: 1070 4922
Bme1580I G_kGCm'C
Cuts at: 1790 2592 3221 4053 5209 1790
Size: 802 629 832 1156 2573
Fragments arranged by size:
2573 1156 832 802 629
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5992
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3150 3723 4681
Size: 416 53 83 399 24 1733 573 958
Cuts at: 4681 442
Size: 1753
Fragments arranged by size:
1753 1733 958 573 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5992
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2189 2228 2842 3005 3043 3148 4652 1239
Size: 950 39 614 163 38 105 1504 2579
Fragments arranged by size:
2579 1504 950 614 163 105 39 38
Bpu10I CC'TnA_GC
Cuts at: 2738 2738
Size: 5992
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4117 4982 5280 4117
Size: 865 298 4829
Fragments arranged by size:
4829 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2447 4655 882
Size: 617 948 2208 2219
Fragments arranged by size:
2219 2208 948 617
BsaAI yAC'GTr
Cuts at: 493 1204 2716 3783 493
Size: 711 1512 1067 2702
Fragments arranged by size:
2702 1512 1067 711
BsaBI GATnn'nnATC
Cuts at: 5986 5986
Size: 5992
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2130 2178 2256 2427
Size: 53 83 186 936 597 48 78 171
Cuts at: 2427 3048 3799 4181 4816 275
Size: 621 751 382 635 1451
Fragments arranged by size:
1451 936 751 635 621 597 382 186
171 83 78 53 48
BsaWI w'CCGG_w
Cuts at: 4425 4823 5097 5244 5655 4425
Size: 398 274 147 411 4762
Fragments arranged by size:
4762 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5767
Fragments arranged by size:
5767 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 2401 3254 1186
Size: 865 350 853 3924
Fragments arranged by size:
3924 865 853 350
BseYI C'CCAG_C
Cuts at: 2031 3065 3147 5195 5691 2031
Size: 1034 82 2048 496 2332
Fragments arranged by size:
2332 2048 1034 496 82
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2050 2912 2993 1163
Size: 573 314 862 81 4162
Fragments arranged by size:
4162 862 573 314 81
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2953 3055 3442 4203 5231 1540
Size: 275 186 952 102 387 761 1028 2301
Fragments arranged by size:
2301 1028 952 761 387 275 186 102
BsiHKAI G_wGCw'C
Cuts at: 3264 4053 4138 5209 3264
Size: 789 85 1071 4047
Fragments arranged by size:
4047 1071 789 85
BsmI GAATG_Cn'
Cuts at: 3503 3596 3503
Size: 93 5899
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2592 2727 2766 2965 3221 3264 3311
Size: 399 802 135 39 199 256 43 47
Cuts at: 3311 4053 4138 5209 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 802 742 399 256 199 135
85 47 43 39
BspEI T'CCGG_A
Cuts at: 5655 5655
Size: 5992
BspHI T'CATG_A
Cuts at: 3881 5611 3881
Size: 1730 4262
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2203 3347 844
Size: 1359 1144 3489
Fragments arranged by size:
3489 1359 1144
BsrBI CCG'CTC
Cuts at: 1394 1823 2439 2887 3879 1394
Size: 429 616 448 992 3507
Fragments arranged by size:
3507 992 616 448 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4487 4661 65
Size: 1183 3239 174 1396
Fragments arranged by size:
3239 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3050 4636 4823 1700
Size: 110 1240 1586 187 2869
Fragments arranged by size:
2869 1586 1240 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5992
BssHII G'CGCG_C
Cuts at: 1925 2047 2219 2407 1925
Size: 122 172 188 5510
Fragments arranged by size:
5510 188 172 122
BssSI C'ACGA_G
Cuts at: 2078 2354 2759 4052 5064 2078
Size: 276 405 1293 1012 3006
Fragments arranged by size:
3006 1293 1012 405 276
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2335 794
Size: 1541 4451
BstBI TT'CG_AA
Cuts at: 4796 4796
Size: 5992
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5992
BstXI CCAn_nnnn'nTGG
Cuts at: 2518 3088 2518
Size: 570 5422
BstYI r'GATC_y
Cuts at: 1972 2751 2784 3322 3689 4074 4091 5543 5629
Size: 779 33 538 367 385 17 1452 86
Cuts at: 5629 5641 5987 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 779 538 385 367 346 86
33 17 12
Bsu36I CC'TnA_GG
Cuts at: 3266 3266
Size: 5992
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5440
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2122 487
Size: 683 56 896 4357
Fragments arranged by size:
4357 896 683 56
BtsI GCAGTG_nn'
Cuts at: 3223 3239 3508 4302 4322 3223
Size: 16 269 794 20 4893
Fragments arranged by size:
4893 794 269 20 16
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5992
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5957
DraI TTT'AAA
Cuts at: 3296 3296
Size: 5992
DraIII CAC_nnn'GTG
Cuts at: 3016 3016
Size: 5992
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4999 809
Size: 507 3683 1802
Fragments arranged by size:
3683 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2160 2445 2508
Size: 54 1425 50 275 18 330 285 63
Cuts at: 2508 3052 3090 3439 4328 5957 8
Size: 544 38 349 889 1629 43
Fragments arranged by size:
1629 1425 889 544 349 330 285 275
63 54 50 43 38 18
EagI C'GGCC_G
Cuts at: 1537 1812 3052 3439 1537
Size: 275 1240 387 4090
Fragments arranged by size:
4090 1240 387 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2805 3252 3922 1353
Size: 1452 447 670 3423
Fragments arranged by size:
3423 1452 670 447
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4579 4953 5099 387
Size: 1064 138 2990 374 146 1280
Fragments arranged by size:
2990 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3262 3262
Size: 5992
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2189 2228 2842 3005 3043 3148 3197
Size: 393 557 39 614 163 38 105 49
Cuts at: 3197 3223 4055 4652 5439 5707 1239
Size: 26 832 597 787 268 1524
Fragments arranged by size:
1524 832 787 614 597 557 393 268
163 105 49 39 38 26
EcoO109I rG'GnC_Cy
Cuts at: 2014 2041 3144 3208 2014
Size: 27 1103 64 4798
Fragments arranged by size:
4798 1103 64 27
EcoRI G'AATT_C
Cuts at: 3301 3679 3301
Size: 378 5614
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2947 2947
Size: 5992
FspI TGC'GCA
Cuts at: 2733 4498 2733
Size: 1765 4227
HaeII r_GCGC'y
Cuts at: 1678 2259 2692 5139 1678
Size: 581 433 2447 2531
Fragments arranged by size:
2531 2447 581 433
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2006 2038 2921 2953 4633
Size: 32 135 32 434 32 883 32 1680
Cuts at: 4633 4665 4707 4739 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1680 883 434 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3336 3582 669
Size: 988 281 1398 246 3079
Fragments arranged by size:
3079 1398 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5992
HpaI GTT'AAC
Cuts at: 3582 3582
Size: 5992
KasI G'GCGC_C
Cuts at: 2255 2255
Size: 5992
KpnI G_GTAC'C
Cuts at: 3317 3317
Size: 5992
MfeI C'AATT_G
Cuts at: 3591 3591
Size: 5992
MluI A'CGCG_T
Cuts at: 5868 5868
Size: 5992
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2553 2787 2862 5106 5290 1305
Size: 1248 234 75 2244 184 2007
Fragments arranged by size:
2244 2007 1248 234 184 75
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2447 2510 10
Size: 54 1425 343 615 63 3492
Fragments arranged by size:
3492 1425 615 343 63 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2583 3950 4309 4468 518
Size: 648 1417 1367 359 159 2042
Fragments arranged by size:
2042 1417 1367 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2054 2092 2236 2335 2464 3408 4085 5233
Size: 448 38 144 99 129 944 677 1148
Cuts at: 5233 5478 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 944 677 448 245 144 129
99 38
NaeI GCC'GGC
Cuts at: 3052 3052
Size: 5992
NarI GG'CG_CC
Cuts at: 2256 2256
Size: 5992
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5440
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5992
NgoMIV G'CCGG_C
Cuts at: 3050 3050
Size: 5992
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5992
NotI GC'GGCC_GC
Cuts at: 3439 3439
Size: 5992
NspI r_CATG'y
Cuts at: 1132 1229 2497 2537 3275 3350 4895 1132
Size: 97 1268 40 738 75 1545 2229
Fragments arranged by size:
2229 1545 1268 738 97 75 40
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5992
PciI A'CATG_T
Cuts at: 2493 4891 2493
Size: 2398 3594
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5992
PfoI T'CCnGG_A
Cuts at: 2226 2858 3020 2226
Size: 632 162 5198
Fragments arranged by size:
5198 632 162
PmeI GTTT'AAAC
Cuts at: 3296 3296
Size: 5992
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4061 4093 5574 5606 4061
Size: 32 1481 32 4447
Fragments arranged by size:
4447 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2014 3144 3208 2014
Size: 1130 64 4798
Fragments arranged by size:
4798 1130 64
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5992
PsiI TTA'TAA
Cuts at: 3562 3562
Size: 5992
PstI C_TGCA'G
Cuts at: 830 2109 2751 3169 3234 3344 830
Size: 1279 642 418 65 110 3478
Fragments arranged by size:
3478 1279 642 418 110 65
PvuI CG_AT'CG
Cuts at: 2001 2953 2001
Size: 952 5040
PvuII CAG'CTG
Cuts at: 2054 2236 2335 2464 2054
Size: 182 99 129 5582
Fragments arranged by size:
5582 182 129 99
SacI G_AGCT'C
Cuts at: 3264 3264
Size: 5992
SalI G'TCGA_C
Cuts at: 1936 3334 1936
Size: 1398 4594
SanDI GG'GwC_CC
Cuts at: 2014 3144 2014
Size: 1130 4862
SapI GCTCTTCn'nnn_
Cuts at: 3252 3252
Size: 5992
SbfI CC_TGCA'GG
Cuts at: 2109 3344 2109
Size: 1235 4757
ScaI AGT'ACT
Cuts at: 4240 5952 4240
Size: 1712 4280
SexAI A'CCwGG_T
Cuts at: 3012 3012
Size: 5992
SfcI C'TryA_G
Cuts at: 826 1045 2105 2747 3165 3230 3340 4475 5156
Size: 219 1060 642 418 65 110 1135 681
Cuts at: 5156 5347 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1060 681 642 418 219 191
110 65
SfoI GGC'GCC
Cuts at: 2257 2257
Size: 5992
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5992
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5992
SmaI CCC'GGG
Cuts at: 1872 2012 2019 2414 2870 3319 1872
Size: 140 7 395 456 449 4545
Fragments arranged by size:
4545 456 449 395 140 7
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3036 4096 4997 5259 820
Size: 197 926 1093 1060 901 262 1553
Fragments arranged by size:
1553 1093 1060 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3783 493
Size: 3290 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5992
SphI G_CATG'C
Cuts at: 1132 2537 3350 1132
Size: 1405 813 3774
Fragments arranged by size:
3774 1405 813
SrfI GCCC'GGGC
Cuts at: 2414 2414
Size: 5992
SspI AAT'ATT
Cuts at: 5 52 3916 5
Size: 47 3864 2081
Fragments arranged by size:
3864 2081 47
StuI AGG'CCT
Cuts at: 1517 3133 1517
Size: 1616 4376
StyI C'CwwG_G
Cuts at: 513 1065 2442 3431 513
Size: 552 1377 989 3074
Fragments arranged by size:
3074 1377 989 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4019 4036 4189 4374 4819 325
Size: 1255 2439 17 153 185 445 1498
Fragments arranged by size:
2439 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4238 5950 96
Size: 274 80 33 51 1443 2261 1712 138
Fragments arranged by size:
2261 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3002 3034 3200 3232 5952 5984 3002
Size: 32 166 32 2720 32 3010
Fragments arranged by size:
3010 2720 166 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3142 1316
Size: 1826 4166
XbaI T'CTAG_A
Cuts at: 3328 3328
Size: 5992
XhoI C'TCGA_G
Cuts at: 1943 3036 1943
Size: 1093 4899
XmaI C'CCGG_G
Cuts at: 1870 2010 2017 2412 2868 3317 1870
Size: 140 7 395 456 449 4545
Fragments arranged by size:
4545 456 449 395 140 7
XmnI GAAnn'nnTTC
Cuts at: 4121 4121
Size: 5992
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2428 3800 276
Size: 53 83 186 936 894 1372 2468
Fragments arranged by size:
2468 1372 936 894 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I EcoNI EcoRV FseI FspAI
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacII SfiI SwaI XcmI