Restriction map for
pFN21AE1897
(Circular) (Six-base) MAPSORT of: pFN21AE1897.seq Check: 3663 from: 1 to: 7114
pFN21AE1897 7114 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4924 278
Size: 53 83 186 936 3388 2468
Fragments arranged by size:
3388 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4457 1937
Size: 2520 4594
Acc65I G'GTAC_C
Cuts at: 2495 3470 4435 2495
Size: 975 965 5174
Fragments arranged by size:
5174 975 965
AclI AA'CG_TT
Cuts at: 1563 5241 5614 1563
Size: 3678 373 3063
Fragments arranged by size:
3678 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2562 3156 3341 4314 5177 6561 6829 1632
Size: 930 594 185 973 863 1384 268 1917
Fragments arranged by size:
1917 1384 973 930 863 594 268 185
AflII C'TTAA_G
Cuts at: 820 1017 3374 3884 820
Size: 197 2357 510 4050
Fragments arranged by size:
4050 2357 510 197
AflIII A'CryG_T
Cuts at: 1203 3515 6013 6990 1203
Size: 2312 2498 977 1327
Fragments arranged by size:
2498 2312 1327 977
AgeI A'CCGG_T
Cuts at: 2453 5945 2453
Size: 3492 3622
AhdI GACnn_n'nnGTC
Cuts at: 5843 5843
Size: 7114
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7114
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7114
AlwNI CAG_nnn'CTG
Cuts at: 3716 3922 4857 6429 3716
Size: 206 935 1572 4401
Fragments arranged by size:
4401 1572 935 206
ApaI G_GGCC'C
Cuts at: 2014 2014
Size: 7114
ApaLI G'TGCA_C
Cuts at: 5171 6327 5171
Size: 1156 5958
ApoI r'AATT_y
Cuts at: 1493 2690 3464 3749 3845 4423 4649 4801 6910
Size: 1197 774 285 96 578 226 152 2109
Cuts at: 6910 1493
Size: 1697
Fragments arranged by size:
2109 1697 1197 774 578 285 226 152
96
AseI AT'TA_AT
Cuts at: 160 1032 4065 5668 160
Size: 872 3033 1603 1606
Fragments arranged by size:
3033 1606 1603 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7114
AvaI C'yCGr_G
Cuts at: 1870 1943 4439 1870
Size: 73 2496 4545
Fragments arranged by size:
4545 2496 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2479 2512 3986 4019 2479
Size: 33 1474 33 5574
Fragments arranged by size:
5574 1474 33 33
BamHI G'GATC_C
Cuts at: 4444 4811 4444
Size: 367 6747
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2495 3470 4435 5890 618
Size: 325 217 627 708 975 965 1455 1842
Fragments arranged by size:
1842 1455 975 965 708 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2014 2880 4281 4433 1391
Size: 623 866 1401 152 4072
Fragments arranged by size:
4072 1401 866 623 152
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3504 3537 4314 4326 928
Size: 971 1605 33 777 12 3716
Fragments arranged by size:
3716 1605 971 777 33 12
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5305 5339 1903
Size: 34 3368 34 3678
Fragments arranged by size:
3678 3368 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2782 5006 6216 2782
Size: 2224 1210 3680
Fragments arranged by size:
3680 2224 1210
BclI T'GATC_A
Cuts at: 1548 3417 4083 1548
Size: 1869 666 4579
Fragments arranged by size:
4579 1869 666
BfrBI ATG'CAT
Cuts at: 2192 2192
Size: 7114
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5725 136
Size: 107 122 71 631 605 337 3716 1525
Fragments arranged by size:
3716 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 7109 7109
Size: 7114
BlpI GC'TnA_GC
Cuts at: 4531 4531
Size: 7114
Bme1580I G_kGCm'C
Cuts at: 1790 2014 5175 6331 1790
Size: 224 3161 1156 2573
Fragments arranged by size:
3161 2573 1156 224
BmgBI CAC'GTC
Cuts at: 1318 3518 1318
Size: 2200 4914
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3010 4845 5803
Size: 416 53 83 399 24 1593 1835 958
Cuts at: 5803 442
Size: 1753
Fragments arranged by size:
1835 1753 1593 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7114
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2698 2698
Size: 7114
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5774 1239
Size: 4535 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5239 6104 6402 5239
Size: 865 298 5951
Fragments arranged by size:
5951 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3031 5777 882
Size: 617 1532 2746 2219
Fragments arranged by size:
2746 2219 1532 617
BsaAI yAC'GTr
Cuts at: 493 1204 3188 4905 493
Size: 711 1984 1717 2702
Fragments arranged by size:
2702 1984 1717 711
BsaBI GATnn'nnATC
Cuts at: 7108 7108
Size: 7114
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4921 5303 5938 275
Size: 53 83 186 936 3388 382 635 1451
Fragments arranged by size:
3388 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2453 5547 5945 6219 6366 6777 2453
Size: 3094 398 274 147 411 2790
Fragments arranged by size:
3094 2790 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3166 3196 3916 3946 1178
Size: 30 165 30 1763 30 720 30 4346
Fragments arranged by size:
4346 1763 720 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2888 1186
Size: 1702 5412
BseYI C'CCAG_C
Cuts at: 2023 2661 3629 6317 6813 2023
Size: 638 968 2688 496 2324
Fragments arranged by size:
2688 2324 968 638 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6541
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4564 5325 6353 1540
Size: 275 186 2563 761 1028 2301
Fragments arranged by size:
2563 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5175 5260 6331 5175
Size: 85 1071 5958
Fragments arranged by size:
5958 1071 85
BsmI GAATG_Cn'
Cuts at: 4233 4625 4718 4233
Size: 392 93 6629
Fragments arranged by size:
6629 392 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2014 2880 4281 4433 5175 5260 6331
Size: 399 224 866 1401 152 742 85 1071
Cuts at: 6331 1391
Size: 2174
Fragments arranged by size:
2174 1401 1071 866 742 399 224 152
85
BspEI T'CCGG_A
Cuts at: 6777 6777
Size: 7114
BspHI T'CATG_A
Cuts at: 3987 5003 6733 3987
Size: 1016 1730 4368
Fragments arranged by size:
4368 1730 1016
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2442 4469 844
Size: 1598 2027 3489
Fragments arranged by size:
3489 2027 1598
BsrBI CCG'CTC
Cuts at: 1394 1823 5001 1394
Size: 429 3178 3507
Fragments arranged by size:
3507 3178 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5609 5783 65
Size: 1183 4361 174 1396
Fragments arranged by size:
4361 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2453 5758 5945 1700
Size: 110 643 3305 187 2869
Fragments arranged by size:
3305 2869 643 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7114
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7114
BssSI C'ACGA_G
Cuts at: 5174 6186 5174
Size: 1012 6102
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7114
BstBI TT'CG_AA
Cuts at: 5918 5918
Size: 7114
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7114
BstYI r'GATC_y
Cuts at: 1972 2104 3209 4138 4444 4811 5196 5213 6665
Size: 132 1105 929 306 367 385 17 1452
Cuts at: 6665 6751 6763 7109 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1105 929 385 367 346 306
132 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2880 3111 513
Size: 552 1815 231 4516
Fragments arranged by size:
4516 1815 552 231
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6375
Fragments arranged by size:
6375 683 56
BtsI GCAGTG_nn'
Cuts at: 4630 5424 5444 4630
Size: 794 20 6300
Fragments arranged by size:
6300 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7114
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7079
DraI TTT'AAA
Cuts at: 2529 4418 2529
Size: 1889 5225
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2053 6121 809
Size: 507 737 4068 1802
Fragments arranged by size:
4068 1802 737 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2557 2581 3948
Size: 54 1425 50 275 18 727 24 1367
Cuts at: 3948 4561 5450 7079 8
Size: 613 889 1629 43
Fragments arranged by size:
1629 1425 1367 889 727 613 275 54
50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 4561 1537
Size: 275 2749 4090
Fragments arranged by size:
4090 2749 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2094 2469 2901 3360 3755 4269 4290 5044
Size: 741 375 432 459 395 514 21 754
Cuts at: 5044 1353
Size: 3423
Fragments arranged by size:
3423 754 741 514 459 432 395 375
21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2596 5701 6075 6221 387
Size: 1064 138 1007 3105 374 146 1280
Fragments arranged by size:
3105 1280 1064 1007 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2562 3156 3341 4314 5177 5774 6561
Size: 393 930 594 185 973 863 597 787
Cuts at: 6561 6829 1239
Size: 268 1524
Fragments arranged by size:
1524 973 930 863 787 597 594 393
268 185
EcoO109I rG'GnC_Cy
Cuts at: 2010 2011 3739 2010
Size: 1 1728 5385
Fragments arranged by size:
5385 1728 1
EcoRI G'AATT_C
Cuts at: 4423 4801 4423
Size: 378 6736
EcoRV GAT'ATC
Cuts at: 3019 3019
Size: 7114
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2251 2251
Size: 7114
FspI TGC'GCA
Cuts at: 5620 5620
Size: 7114
HaeII r_GCGC'y
Cuts at: 1678 3922 6261 1678
Size: 2244 2339 2531
Fragments arranged by size:
2531 2339 2244
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2546 2578 3638 3670 3916
Size: 32 135 32 974 32 1060 32 246
Cuts at: 3916 3948 5755 5787 5829 5861 1373
Size: 32 1807 32 42 32 2626
Fragments arranged by size:
2626 1807 1060 974 246 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2251 2565 4458 4704 669
Size: 988 281 313 314 1893 246 3079
Fragments arranged by size:
3079 1893 988 314 313 281 246
HindIII A'AGCT_T
Cuts at: 748 3072 3392 3482 748
Size: 2324 320 90 4380
Fragments arranged by size:
4380 2324 320 90
HpaI GTT'AAC
Cuts at: 2251 4704 2251
Size: 2453 4661
KpnI G_GTAC'C
Cuts at: 2499 3474 4439 2499
Size: 975 965 5174
Fragments arranged by size:
5174 975 965
MfeI C'AATT_G
Cuts at: 4713 4713
Size: 7114
MluI A'CGCG_T
Cuts at: 6990 6990
Size: 7114
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2157 2202 3581 6228 6412 1305
Size: 852 45 1379 2647 184 2007
Fragments arranged by size:
2647 2007 1379 852 184 45
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2559 2583 3950 10
Size: 54 1425 343 727 24 1367 3174
Fragments arranged by size:
3174 1425 1367 727 343 54 24
MslI CAynn'nnrTG
Cuts at: 518 1166 5072 5431 5590 518
Size: 648 3906 359 159 2042
Fragments arranged by size:
3906 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4081 4397 4530 5207 6355 6600 1606
Size: 2475 316 133 677 1148 245 2120
Fragments arranged by size:
2475 2120 1148 677 316 245 133
NcoI C'CATG_G
Cuts at: 513 1065 2880 3111 513
Size: 552 1815 231 4516
Fragments arranged by size:
4516 1815 552 231
NdeI CA'TA_TG
Cuts at: 387 4207 387
Size: 3820 3294
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7114
NotI GC'GGCC_GC
Cuts at: 4561 4561
Size: 7114
NsiI A_TGCA'T
Cuts at: 2194 2194
Size: 7114
NspI r_CATG'y
Cuts at: 1132 1229 2444 4096 4472 6017 1132
Size: 97 1215 1652 376 1545 2229
Fragments arranged by size:
2229 1652 1545 1215 376 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7114
PciI A'CATG_T
Cuts at: 6013 6013
Size: 7114
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3586 1108
Size: 2478 4636
PmeI GTTT'AAAC
Cuts at: 4418 4418
Size: 7114
PmlI CAC'GTG
Cuts at: 3188 3188
Size: 7114
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5183 5215 6696 6728 5183
Size: 32 1481 32 5569
Fragments arranged by size:
5569 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7114
PsiI TTA'TAA
Cuts at: 4684 4684
Size: 7114
PspOMI G'GGCC_C
Cuts at: 2010 2010
Size: 7114
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2728 2760 2728
Size: 32 7082
PstI C_TGCA'G
Cuts at: 830 2938 4466 830
Size: 2108 1528 3478
Fragments arranged by size:
3478 2108 1528
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7114
PvuII CAG'CTG
Cuts at: 4397 4397
Size: 7114
SalI G'TCGA_C
Cuts at: 1936 4456 1936
Size: 2520 4594
SapI GCTCTTCn'nnn_
Cuts at: 4269 4269
Size: 7114
SbfI CC_TGCA'GG
Cuts at: 4466 4466
Size: 7114
ScaI AGT'ACT
Cuts at: 5362 7074 5362
Size: 1712 5402
SfcI C'TryA_G
Cuts at: 826 1045 2934 4392 4462 5597 6278 6469 826
Size: 219 1889 1458 70 1135 681 191 1471
Fragments arranged by size:
1889 1471 1458 1135 681 219 191 70
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7114
SgrAI Cr'CCGG_yG
Cuts at: 1700 2453 1700
Size: 753 6361
SmaI CCC'GGG
Cuts at: 1872 4441 1872
Size: 2569 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3374 3884 5218 6119 6381 820
Size: 197 926 1431 510 1334 901 262 1553
Fragments arranged by size:
1553 1431 1334 926 901 510 262 197
SnaBI TAC'GTA
Cuts at: 493 4905 493
Size: 4412 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7114
SphI G_CATG'C
Cuts at: 1132 4472 1132
Size: 3340 3774
SspI AAT'ATT
Cuts at: 5 52 2816 5038 5
Size: 47 2764 2222 2081
Fragments arranged by size:
2764 2222 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7114
StyI C'CwwG_G
Cuts at: 513 1065 2491 2880 3111 3780 4553 513
Size: 552 1426 389 231 669 773 3074
Fragments arranged by size:
3074 1426 773 669 552 389 231
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2008 2047 4164 5141 5158 5311 5496
Size: 1255 428 39 2117 977 17 153 185
Cuts at: 5496 5941 325
Size: 445 1498
Fragments arranged by size:
2117 1498 1255 977 445 428 185 153
39 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2149 5360 7072
Size: 274 80 33 51 1443 172 3211 1712
Cuts at: 7072 96
Size: 138
Fragments arranged by size:
3211 1712 1443 274 172 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3166 3198 7074 7106 3166
Size: 32 3876 32 3174
Fragments arranged by size:
3876 3174 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7114
XbaI T'CTAG_A
Cuts at: 4450 4450
Size: 7114
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2476 2476
Size: 7114
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7114
XmaI C'CCGG_G
Cuts at: 1870 4439 1870
Size: 2569 4545
XmnI GAAnn'nnTTC
Cuts at: 2169 3764 5243 2169
Size: 1595 1479 4040
Fragments arranged by size:
4040 1595 1479
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4922 276
Size: 53 83 186 936 3388 2468
Fragments arranged by size:
3388 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI Bpu10I
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PfoI PpuMI PspXI RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI