Restriction map for
pFN21AE1895
(Circular) (Six-base) MAPSORT of: pFN21AE1895.seq Check: 9914 from: 1 to: 5956
pFN21AE1895 5956 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2219 2878 2219
Size: 659 5297
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3766 278
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3299 1937
Size: 1362 4594
Acc65I G'GTAC_C
Cuts at: 3277 3277
Size: 5956
AclI AA'CG_TT
Cuts at: 1563 4083 4456 1563
Size: 2520 373 3063
Fragments arranged by size:
3063 2520 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2195 4019 5403 5671 1632
Size: 563 1824 1384 268 1917
Fragments arranged by size:
1917 1824 1384 563 268
AfeI AGC'GCT
Cuts at: 2573 3146 2573
Size: 573 5383
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5759
AflIII A'CryG_T
Cuts at: 1203 4855 5832 1203
Size: 3652 977 1327
Fragments arranged by size:
3652 1327 977
AgeI A'CCGG_T
Cuts at: 4787 4787
Size: 5956
AhdI GACnn_n'nnGTC
Cuts at: 4685 4685
Size: 5956
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5956
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3257 1184
Size: 2073 3883
AlwNI CAG_nnn'CTG
Cuts at: 2154 3699 5271 2154
Size: 1545 1572 2839
Fragments arranged by size:
2839 1572 1545
ApaI G_GGCC'C
Cuts at: 2071 2077 2443 3002 2071
Size: 6 366 559 5025
Fragments arranged by size:
5025 559 366 6
ApaLI G'TGCA_C
Cuts at: 4013 5169 4013
Size: 1156 4800
ApoI r'AATT_y
Cuts at: 1493 3265 3491 3643 5752 1493
Size: 1772 226 152 2109 1697
Fragments arranged by size:
2109 1772 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4510 160
Size: 872 3478 1606
Fragments arranged by size:
3478 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
AvaI C'yCGr_G
Cuts at: 1870 1943 2070 2861 3281 1870
Size: 73 127 791 420 4545
Fragments arranged by size:
4545 791 420 127 73
BamHI G'GATC_C
Cuts at: 3286 3653 3286
Size: 367 5589
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3150 3277 4732 618
Size: 325 217 627 1363 127 1455 1842
Fragments arranged by size:
1842 1455 1363 627 325 217 127
BanII G_rGCy'C
Cuts at: 1391 2071 2077 2196 2443 2926 3002 3275 1391
Size: 680 6 119 247 483 76 273 4072
Fragments arranged by size:
4072 680 483 273 247 119 76 6
BbeI G_GCGC'C
Cuts at: 3154 3154
Size: 5956
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3231 928
Size: 971 1332 3653
Fragments arranged by size:
3653 1332 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4147 4181 1903
Size: 34 2210 34 3678
Fragments arranged by size:
3678 2210 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3848 5058 3848
Size: 1210 4746
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5956
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2505 4567 136
Size: 107 122 71 631 605 833 2062 1525
Fragments arranged by size:
2062 1525 833 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2814 5951 2814
Size: 3137 2819
BlpI GC'TnA_GC
Cuts at: 3373 3373
Size: 5956
Bme1580I G_kGCm'C
Cuts at: 1790 2071 2077 2169 2275 2443 3002 4017 5173
Size: 281 6 92 106 168 559 1015 1156
Cuts at: 5173 1790
Size: 2573
Fragments arranged by size:
2573 1156 1015 559 281 168 106 92
6
BmgBI CAC'GTC
Cuts at: 1318 3016 1318
Size: 1698 4258
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3687 4645 442
Size: 416 53 83 399 24 2270 958 1753
Fragments arranged by size:
2270 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5956
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2317 2317
Size: 5956
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2336 2614 3215 4616 1239
Size: 1097 278 601 1401 2579
Fragments arranged by size:
2579 1401 1097 601 278
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3125 4081 4946 5244 3125
Size: 956 865 298 3837
Fragments arranged by size:
3837 956 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2858 3114 4619 882
Size: 617 1359 256 1505 2219
Fragments arranged by size:
2219 1505 1359 617 256
BsaAI yAC'GTr
Cuts at: 493 1204 3747 493
Size: 711 2543 2702
Fragments arranged by size:
2702 2543 711
BsaBI GATnn'nnATC
Cuts at: 5950 5950
Size: 5956
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3151 3763 4145 4780
Size: 53 83 186 936 1618 612 382 635
Cuts at: 4780 275
Size: 1451
Fragments arranged by size:
1618 1451 936 635 612 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4389 4787 5061 5208 5619 4389
Size: 398 274 147 411 4726
Fragments arranged by size:
4726 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5731
Fragments arranged by size:
5731 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2039 1186
Size: 853 5103
BseYI C'CCAG_C
Cuts at: 2062 2158 2720 2915 2965 3031 3064 5159 5655
Size: 96 562 195 50 66 33 2095 496
Cuts at: 5655 2062
Size: 2363
Fragments arranged by size:
2363 2095 562 496 195 96 66 50
33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2429 2803 1163
Size: 573 693 374 4316
Fragments arranged by size:
4316 693 573 374
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2018 3112 3406 4167 5195 1540
Size: 275 186 17 1094 294 761 1028 2301
Fragments arranged by size:
2301 1094 1028 761 294 275 186 17
BsiHKAI G_wGCw'C
Cuts at: 2552 2622 2926 4017 4102 5173 2552
Size: 70 304 1091 85 1071 3335
Fragments arranged by size:
3335 1091 1071 304 85 70
BsmI GAATG_Cn'
Cuts at: 3467 3560 3467
Size: 93 5863
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2071 2077 2169 2196 2275 2443 2552
Size: 399 281 6 92 27 79 168 109
Cuts at: 2552 2622 2926 3002 3275 4017 4102 5173 1391
Size: 70 304 76 273 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 304 281 273 168
109 92 85 79 76 70 27 6
BspEI T'CCGG_A
Cuts at: 5619 5619
Size: 5956
BspHI T'CATG_A
Cuts at: 3845 5575 3845
Size: 1730 4226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2219 2504 2878 3311 844
Size: 1375 285 374 433 3489
Fragments arranged by size:
3489 1375 433 374 285
BsrBI CCG'CTC
Cuts at: 1394 1823 3843 1394
Size: 429 2020 3507
Fragments arranged by size:
3507 2020 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4451 4625 65
Size: 1183 3203 174 1396
Fragments arranged by size:
3203 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2111 2746 2969 4600 4787 1700
Size: 110 301 635 223 1631 187 2869
Fragments arranged by size:
2869 1631 635 301 223 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5956
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5956
BssSI C'ACGA_G
Cuts at: 2545 4016 5028 2545
Size: 1471 1012 3473
Fragments arranged by size:
3473 1471 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2154 794
Size: 1360 4596
BstBI TT'CG_AA
Cuts at: 4760 4760
Size: 5956
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5956
BstYI r'GATC_y
Cuts at: 1972 2814 3286 3653 4038 4055 5507 5593 5605
Size: 842 472 367 385 17 1452 86 12
Cuts at: 5605 5951 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 842 472 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2018 3093 3165 513
Size: 552 939 14 1075 72 3304
Fragments arranged by size:
3304 1075 939 552 72 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2538 487
Size: 683 56 1312 3905
Fragments arranged by size:
3905 1312 683 56
BtsI GCAGTG_nn'
Cuts at: 2337 2680 2812 3472 4266 4286 2337
Size: 343 132 660 794 20 4007
Fragments arranged by size:
4007 794 660 343 132 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5956
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2197 2232 529
Size: 35 1633 35 4253
Fragments arranged by size:
4253 1633 35 35
DraI TTT'AAA
Cuts at: 3260 3260
Size: 5956
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4963 809
Size: 507 3647 1802
Fragments arranged by size:
3647 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2015 2334 2748
Size: 54 1425 50 275 18 185 319 414
Cuts at: 2748 3109 3403 4292 5921 8
Size: 361 294 889 1629 43
Fragments arranged by size:
1629 1425 889 414 361 319 294 275
185 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2015 3109 3403 1537
Size: 275 203 1094 294 4090
Fragments arranged by size:
4090 1094 294 275 203
EarI CTCTTCn'nnn_
Cuts at: 1353 3886 1353
Size: 2533 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4543 4917 5063 387
Size: 1064 138 2954 374 146 1280
Fragments arranged by size:
2954 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2924 2924
Size: 5956
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2195 2336 2614 3215 4019 4616 5403
Size: 393 563 141 278 601 804 597 787
Cuts at: 5403 5671 1239
Size: 268 1524
Fragments arranged by size:
1524 804 787 601 597 563 393 278
268 141
EcoO109I rG'GnC_Cy
Cuts at: 2067 2179 2439 2440 2493 2067
Size: 112 260 1 53 5530
Fragments arranged by size:
5530 260 112 53 1
EcoRI G'AATT_C
Cuts at: 3265 3643 3265
Size: 378 5578
FspI TGC'GCA
Cuts at: 3059 3233 4462 3059
Size: 174 1229 4553
Fragments arranged by size:
4553 1229 174
FspAI rTGC'GCAy
Cuts at: 3059 3059
Size: 5956
HaeII r_GCGC'y
Cuts at: 1678 2048 2315 2575 2778 2879 3043 3148 3154
Size: 370 267 260 203 101 164 105 6
Cuts at: 3154 5103 1678
Size: 1949 2531
Fragments arranged by size:
2531 1949 370 267 260 203 164 105
101 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2716 2748 4597 4629 4671
Size: 32 135 32 1144 32 1849 32 42
Cuts at: 4671 4703 1373
Size: 32 2626
Fragments arranged by size:
2626 1849 1144 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3300 3546 669
Size: 988 281 1362 246 3079
Fragments arranged by size:
3079 1362 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5956
HpaI GTT'AAC
Cuts at: 3546 3546
Size: 5956
KasI G'GCGC_C
Cuts at: 3150 3150
Size: 5956
KpnI G_GTAC'C
Cuts at: 3281 3281
Size: 5956
MfeI C'AATT_G
Cuts at: 3555 3555
Size: 5956
MluI A'CGCG_T
Cuts at: 5832 5832
Size: 5956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5070 5254 1305
Size: 3765 184 2007
Fragments arranged by size:
3765 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4134
Fragments arranged by size:
4134 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2523 3914 4273 4432 518
Size: 648 1357 1391 359 159 2042
Fragments arranged by size:
2042 1391 1357 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2014 2020 2162 2277 2346 2582 2844 2936
Size: 408 6 142 115 69 236 262 92
Cuts at: 2936 3167 3372 4049 5197 5442 1606
Size: 231 205 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 408 262 245 236 231
205 142 115 92 69 6
NaeI GCC'GGC
Cuts at: 2971 2971
Size: 5956
NarI GG'CG_CC
Cuts at: 3151 3151
Size: 5956
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4465
Fragments arranged by size:
4465 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5956
NgoMIV G'CCGG_C
Cuts at: 2969 2969
Size: 5956
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5956
NotI GC'GGCC_GC
Cuts at: 2015 3403 2015
Size: 1388 4568
NspI r_CATG'y
Cuts at: 1132 1229 3314 4859 1132
Size: 97 2085 1545 2229
Fragments arranged by size:
2229 2085 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2077 2554 1348
Size: 729 477 4750
Fragments arranged by size:
4750 729 477
PciI A'CATG_T
Cuts at: 4855 4855
Size: 5956
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2833 1108
Size: 1725 4231
PmeI GTTT'AAAC
Cuts at: 3260 3260
Size: 5956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4025 4057 5538 5570 4025
Size: 32 1481 32 4411
Fragments arranged by size:
4411 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2493 2493
Size: 5956
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5956
PsiI TTA'TAA
Cuts at: 3526 3526
Size: 5956
PspOMI G'GGCC_C
Cuts at: 2067 2073 2439 2998 2067
Size: 6 366 559 5025
Fragments arranged by size:
5025 559 366 6
PstI C_TGCA'G
Cuts at: 830 2713 2994 3246 3308 830
Size: 1883 281 252 62 3478
Fragments arranged by size:
3478 1883 281 252 62
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5956
PvuII CAG'CTG
Cuts at: 2844 2844
Size: 5956
SacI G_AGCT'C
Cuts at: 2926 2926
Size: 5956
SacII CC_GC'GG
Cuts at: 2021 3168 2021
Size: 1147 4809
SalI G'TCGA_C
Cuts at: 1936 3298 1936
Size: 1362 4594
SbfI CC_TGCA'GG
Cuts at: 3308 3308
Size: 5956
ScaI AGT'ACT
Cuts at: 4204 5916 4204
Size: 1712 4244
SfcI C'TryA_G
Cuts at: 826 1045 2709 2990 3242 3304 4439 5120 5311
Size: 219 1664 281 252 62 1135 681 191
Cuts at: 5311 826
Size: 1471
Fragments arranged by size:
1664 1471 1135 681 281 252 219 191
62
SfoI GGC'GCC
Cuts at: 3152 3152
Size: 5956
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5956
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5956
SmaI CCC'GGG
Cuts at: 1872 2072 3283 1872
Size: 200 1211 4545
Fragments arranged by size:
4545 1211 200
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3140 4060 4961 5223 820
Size: 197 926 1197 920 901 262 1553
Fragments arranged by size:
1553 1197 926 920 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3747 493
Size: 3254 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5956
SphI G_CATG'C
Cuts at: 1132 3314 1132
Size: 2182 3774
SrfI GCCC'GGGC
Cuts at: 2072 2072
Size: 5956
SspI AAT'ATT
Cuts at: 5 52 3880 5
Size: 47 3828 2081
Fragments arranged by size:
3828 2081 47
StuI AGG'CCT
Cuts at: 1517 2285 2300 1517
Size: 768 15 5173
Fragments arranged by size:
5173 768 15
StyI C'CwwG_G
Cuts at: 513 1065 2004 2766 3395 513
Size: 552 939 762 629 3074
Fragments arranged by size:
3074 939 762 629 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2768 3983 4000 4153 4338 4783 325
Size: 1255 1188 1215 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1215 1188 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4202 5914 96
Size: 274 80 33 51 1443 2225 1712 138
Fragments arranged by size:
2225 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5916 5948 5916
Size: 32 5924
Tth111I GACn'n_nGTC
Cuts at: 1316 2896 3026 1316
Size: 1580 130 4246
Fragments arranged by size:
4246 1580 130
XbaI T'CTAG_A
Cuts at: 3292 3292
Size: 5956
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2129 2551 3039 2129
Size: 422 488 5046
Fragments arranged by size:
5046 488 422
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5956
XmaI C'CCGG_G
Cuts at: 1870 2070 3281 1870
Size: 200 1211 4545
Fragments arranged by size:
4545 1211 200
XmnI GAAnn'nnTTC
Cuts at: 3050 4085 3050
Size: 1035 4921
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3764 276
Size: 53 83 186 936 2230 2468
Fragments arranged by size:
2468 2230 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI NruI NsiI PacI PfoI PmlI PspXI PsrI
RsrII SanDI SapI SexAI SfiI SwaI