Restriction map for
pFN21AE1894
(Circular) (Six-base) MAPSORT of: pFN21AE1894.seq Check: 5782 from: 1 to: 5974
pFN21AE1894 5974 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3784 278
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3317 1937
Size: 1380 4594
Acc65I G'GTAC_C
Cuts at: 3295 3295
Size: 5974
AclI AA'CG_TT
Cuts at: 1563 4101 4474 1563
Size: 2538 373 3063
Fragments arranged by size:
3063 2538 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2222 2822 2912 4037 5421 5689 1632
Size: 590 600 90 1125 1384 268 1917
Fragments arranged by size:
1917 1384 1125 600 590 268 90
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5777
AflIII A'CryG_T
Cuts at: 1203 2134 4873 5850 1203
Size: 931 2739 977 1327
Fragments arranged by size:
2739 1327 977 931
AgeI A'CCGG_T
Cuts at: 4805 4805
Size: 5974
AhdI GACnn_n'nnGTC
Cuts at: 4703 4703
Size: 5974
AleI CACnn'nnGTG
Cuts at: 1166 2792 1166
Size: 1626 4348
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5974
AlwNI CAG_nnn'CTG
Cuts at: 3717 5289 3717
Size: 1572 4402
ApaI G_GGCC'C
Cuts at: 2278 2278
Size: 5974
ApaLI G'TGCA_C
Cuts at: 4031 5187 4031
Size: 1156 4818
ApoI r'AATT_y
Cuts at: 1493 2346 3283 3509 3661 5770 1493
Size: 853 937 226 152 2109 1697
Fragments arranged by size:
2109 1697 937 853 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4528 160
Size: 872 3496 1606
Fragments arranged by size:
3496 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
AvaI C'yCGr_G
Cuts at: 1870 1943 2959 3299 1870
Size: 73 1016 340 4545
Fragments arranged by size:
4545 1016 340 73
BamHI G'GATC_C
Cuts at: 3304 3671 3304
Size: 367 5607
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2725 3295 4750 618
Size: 325 217 627 938 570 1455 1842
Fragments arranged by size:
1842 1455 938 627 570 325 217
BanII G_rGCy'C
Cuts at: 1391 2040 2278 2937 3293 1391
Size: 649 238 659 356 4072
Fragments arranged by size:
4072 659 649 356 238
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5003
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2483 2517 4165 4199 1903
Size: 34 546 34 1648 34 3678
Fragments arranged by size:
3678 1648 546 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3866 5076 3866
Size: 1210 4764
BclI T'GATC_A
Cuts at: 1548 2589 2842 3207 1548
Size: 1041 253 365 4315
Fragments arranged by size:
4315 1041 365 253
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4585 136
Size: 107 122 71 631 605 2913 1525
Fragments arranged by size:
2913 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2584 5969 2584
Size: 3385 2589
BlpI GC'TnA_GC
Cuts at: 3391 3391
Size: 5974
Bme1580I G_kGCm'C
Cuts at: 1790 2278 2730 3113 4035 5191 1790
Size: 488 452 383 922 1156 2573
Fragments arranged by size:
2573 1156 922 488 452 383
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5974
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3705 4663 442
Size: 416 53 83 399 24 2288 958 1753
Fragments arranged by size:
2288 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5974
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2925 2925
Size: 5974
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2933 2975 4634 1239
Size: 1694 42 1659 2579
Fragments arranged by size:
2579 1694 1659 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2297 2722 4099 4964 5262 2297
Size: 425 1377 865 298 3009
Fragments arranged by size:
3009 1377 865 425 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2879 4637 882
Size: 617 1380 1758 2219
Fragments arranged by size:
2219 1758 1380 617
BsaAI yAC'GTr
Cuts at: 493 1204 2137 2554 3765 493
Size: 711 933 417 1211 2702
Fragments arranged by size:
2702 1211 933 711 417
BsaBI GATnn'nnATC
Cuts at: 2682 5968 2682
Size: 3286 2688
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3781 4163 4798 275
Size: 53 83 186 936 2248 382 635 1451
Fragments arranged by size:
2248 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4407 4805 5079 5226 5637 4407
Size: 398 274 147 411 4744
Fragments arranged by size:
4744 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2000 2030 2789 2819 1178
Size: 30 165 30 597 30 759 30 4333
Fragments arranged by size:
4333 759 597 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2111 2789 3119 1186
Size: 925 678 330 4041
Fragments arranged by size:
4041 925 678 330
BseYI C'CCAG_C
Cuts at: 2048 2995 5177 5673 2048
Size: 947 2182 496 2349
Fragments arranged by size:
2349 2182 947 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2770 1163
Size: 573 1034 4367
Fragments arranged by size:
4367 1034 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3424 4185 5213 1540
Size: 275 186 1423 761 1028 2301
Fragments arranged by size:
2301 1423 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2937 4035 4120 5191 2937
Size: 1098 85 1071 3720
Fragments arranged by size:
3720 1098 1071 85
BsmI GAATG_Cn'
Cuts at: 3485 3578 3485
Size: 93 5881
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2278 2730 2937 3113 3293 4035
Size: 399 250 238 452 207 176 180 742
Cuts at: 4035 4120 5191 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 452 399 250 238 207
180 176 85
BspEI T'CCGG_A
Cuts at: 5637 5637
Size: 5974
BspHI T'CATG_A
Cuts at: 3243 3863 5593 3243
Size: 620 1730 3624
Fragments arranged by size:
3624 1730 620
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2949 3329 844
Size: 2105 380 3489
Fragments arranged by size:
3489 2105 380
BsrBI CCG'CTC
Cuts at: 1394 1823 3861 1394
Size: 429 2038 3507
Fragments arranged by size:
3507 2038 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2821 4469 4643 65
Size: 1183 1573 1648 174 1396
Fragments arranged by size:
1648 1573 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4618 4805 1700
Size: 110 2808 187 2869
Fragments arranged by size:
2869 2808 187 110
BsrGI T'GTAC_A
Cuts at: 96 2184 96
Size: 2088 3886
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5974
BssSI C'ACGA_G
Cuts at: 4034 5046 4034
Size: 1012 4962
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2371 3125 794
Size: 1577 754 3643
Fragments arranged by size:
3643 1577 754
BstBI TT'CG_AA
Cuts at: 4778 4778
Size: 5974
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5974
BstXI CCAn_nnnn'nTGG
Cuts at: 2254 2254
Size: 5974
BstYI r'GATC_y
Cuts at: 1972 2584 3304 3671 4056 4073 5525 5611 5623
Size: 612 720 367 385 17 1452 86 12
Cuts at: 5623 5969 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 720 612 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2790 513
Size: 552 939 786 3697
Fragments arranged by size:
3697 939 786 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2409 487
Size: 683 56 1183 4052
Fragments arranged by size:
4052 1183 683 56
BtsI GCAGTG_nn'
Cuts at: 3490 4284 4304 3490
Size: 794 20 5160
Fragments arranged by size:
5160 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5974
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5939
DraI TTT'AAA
Cuts at: 2644 3278 2644
Size: 634 5340
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4981 809
Size: 507 3665 1802
Fragments arranged by size:
3665 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3421 4310 5939
Size: 54 1425 50 275 18 1591 889 1629
Cuts at: 5939 8
Size: 43
Fragments arranged by size:
1629 1591 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3421 1537
Size: 275 1609 4090
Fragments arranged by size:
4090 1609 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2834 3904 1353
Size: 1481 1070 3423
Fragments arranged by size:
3423 1481 1070
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2146 4561 4935 5081 387
Size: 1064 138 557 2415 374 146 1280
Fragments arranged by size:
2415 1280 1064 557 374 146 138
EcoICRI GAG'CTC
Cuts at: 2935 2935
Size: 5974
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2222 2822 2912 2933 2975 4037 4634
Size: 393 590 600 90 21 42 1062 597
Cuts at: 4634 5421 5689 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1062 787 600 597 590 393 268
90 42 21
EcoO109I rG'GnC_Cy
Cuts at: 2206 2274 2596 3195 2206
Size: 68 322 599 4985
Fragments arranged by size:
4985 599 322 68
EcoRI G'AATT_C
Cuts at: 3283 3661 3283
Size: 378 5596
FspI TGC'GCA
Cuts at: 2375 4480 2375
Size: 2105 3869
HaeII r_GCGC'y
Cuts at: 1678 5121 1678
Size: 3443 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2588 2620 2728 2760 2765
Size: 32 135 32 1016 32 108 32 5
Cuts at: 2765 2797 4615 4647 4689 4721 1373
Size: 32 1818 32 42 32 2626
Fragments arranged by size:
2626 1818 1016 135 108 42 32 32
32 32 32 32 32 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3318 3564 669
Size: 988 281 1380 246 3079
Fragments arranged by size:
3079 1380 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5974
HpaI GTT'AAC
Cuts at: 3564 3564
Size: 5974
KpnI G_GTAC'C
Cuts at: 3299 3299
Size: 5974
MfeI C'AATT_G
Cuts at: 3573 3573
Size: 5974
MluI A'CGCG_T
Cuts at: 5850 5850
Size: 5974
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5088 5272 1305
Size: 3783 184 2007
Fragments arranged by size:
3783 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4152
Fragments arranged by size:
4152 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2792 3932 4291 4450 518
Size: 648 1626 1140 359 159 2042
Fragments arranged by size:
2042 1626 1140 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2407 2525 2995 3390 4067 5215 5460 1606
Size: 801 118 470 395 677 1148 245 2120
Fragments arranged by size:
2120 1148 801 677 470 395 245 118
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4483
Fragments arranged by size:
4483 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5974
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5974
NotI GC'GGCC_GC
Cuts at: 3421 3421
Size: 5974
NspI r_CATG'y
Cuts at: 1132 1229 3332 4877 1132
Size: 97 2103 1545 2229
Fragments arranged by size:
2229 2103 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5974
PciI A'CATG_T
Cuts at: 4873 4873
Size: 5974
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5974
PfoI T'CCnGG_A
Cuts at: 2766 2766
Size: 5974
PmeI GTTT'AAAC
Cuts at: 3278 3278
Size: 5974
PmlI CAC'GTG
Cuts at: 2137 2137
Size: 5974
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4043 4075 5556 5588 4043
Size: 32 1481 32 4429
Fragments arranged by size:
4429 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2596 2596
Size: 5974
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5974
PsiI TTA'TAA
Cuts at: 3544 3544
Size: 5974
PspOMI G'GGCC_C
Cuts at: 2274 2274
Size: 5974
PstI C_TGCA'G
Cuts at: 830 2406 2530 3326 830
Size: 1576 124 796 3478
Fragments arranged by size:
3478 1576 796 124
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5974
PvuII CAG'CTG
Cuts at: 2407 2525 2995 2407
Size: 118 470 5386
Fragments arranged by size:
5386 470 118
SacI G_AGCT'C
Cuts at: 2937 2937
Size: 5974
SalI G'TCGA_C
Cuts at: 1936 3316 1936
Size: 1380 4594
SbfI CC_TGCA'GG
Cuts at: 3326 3326
Size: 5974
ScaI AGT'ACT
Cuts at: 4222 5934 4222
Size: 1712 4262
SfcI C'TryA_G
Cuts at: 826 1045 2402 2526 3219 3322 4457 5138 5329
Size: 219 1357 124 693 103 1135 681 191
Cuts at: 5329 826
Size: 1471
Fragments arranged by size:
1471 1357 1135 693 681 219 191 124
103
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5974
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5974
SmaI CCC'GGG
Cuts at: 1872 3301 1872
Size: 1429 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2312 2701 2959 4078 4979 5241
Size: 197 926 369 389 258 1119 901 262
Cuts at: 5241 820
Size: 1553
Fragments arranged by size:
1553 1119 926 901 389 369 262 258
197
SnaBI TAC'GTA
Cuts at: 493 3765 493
Size: 3272 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5974
SphI G_CATG'C
Cuts at: 1132 3332 1132
Size: 2200 3774
SspI AAT'ATT
Cuts at: 5 52 3898 5
Size: 47 3846 2081
Fragments arranged by size:
3846 2081 47
StuI AGG'CCT
Cuts at: 1517 2310 2498 1517
Size: 793 188 4993
Fragments arranged by size:
4993 793 188
StyI C'CwwG_G
Cuts at: 513 1065 2004 2253 3413 513
Size: 552 939 249 1160 3074
Fragments arranged by size:
3074 1160 939 552 249
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2139 3242 4001 4018 4171 4356 4801
Size: 1255 559 1103 759 17 153 185 445
Cuts at: 4801 325
Size: 1498
Fragments arranged by size:
1498 1255 1103 759 559 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2184 2805 4220
Size: 274 80 33 51 1443 207 621 1415
Cuts at: 4220 5932 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1415 621 274 207 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2817 2849 5934 5966 2817
Size: 32 3085 32 2825
Fragments arranged by size:
3085 2825 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5974
XbaI T'CTAG_A
Cuts at: 3310 3310
Size: 5974
XhoI C'TCGA_G
Cuts at: 1943 2959 1943
Size: 1016 4958
XmaI C'CCGG_G
Cuts at: 1870 3299 1870
Size: 1429 4545
XmnI GAAnn'nnTTC
Cuts at: 3236 4103 3236
Size: 867 5107
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3782 276
Size: 53 83 186 936 2248 2468
Fragments arranged by size:
2468 2248 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI