Restriction map for
pFN21AE1887
(Circular) (Six-base) MAPSORT of: pFN21AE1887.seq Check: 292 from: 1 to: 5944
pFN21AE1887 5944 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2076 3754 278
Size: 53 83 186 936 540 1678 2468
Fragments arranged by size:
2468 1678 936 540 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2658 3287 1937
Size: 721 629 4594
Fragments arranged by size:
4594 721 629
Acc65I G'GTAC_C
Cuts at: 3265 3265
Size: 5944
AclI AA'CG_TT
Cuts at: 1563 4071 4444 1563
Size: 2508 373 3063
Fragments arranged by size:
3063 2508 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4007 5391 5659 1632
Size: 2375 1384 268 1917
Fragments arranged by size:
2375 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5747
AflIII A'CryG_T
Cuts at: 1203 4843 5820 1203
Size: 3640 977 1327
Fragments arranged by size:
3640 1327 977
AgeI A'CCGG_T
Cuts at: 4775 4775
Size: 5944
AhdI GACnn_n'nnGTC
Cuts at: 4673 4673
Size: 5944
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5944
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5944
AlwNI CAG_nnn'CTG
Cuts at: 2434 3687 5259 2434
Size: 1253 1572 3119
Fragments arranged by size:
3119 1572 1253
ApaLI G'TGCA_C
Cuts at: 4001 5157 4001
Size: 1156 4788
ApoI r'AATT_y
Cuts at: 1493 3253 3479 3631 5740 1493
Size: 1760 226 152 2109 1697
Fragments arranged by size:
2109 1760 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4498 160
Size: 872 3466 1606
Fragments arranged by size:
3466 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5944
AvaI C'yCGr_G
Cuts at: 1870 1943 2015 2454 3269 1870
Size: 73 72 439 815 4545
Fragments arranged by size:
4545 815 439 73 72
BamHI G'GATC_C
Cuts at: 3274 3641 3274
Size: 367 5577
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2041 2263 3265 4720 618
Size: 325 217 627 254 222 1002 1455 1842
Fragments arranged by size:
1842 1455 1002 627 325 254 222 217
BanII G_rGCy'C
Cuts at: 1391 2993 3263 1391
Size: 1602 270 4072
Fragments arranged by size:
4072 1602 270
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2644 3030 3147 3165 928
Size: 971 745 386 117 18 3707
Fragments arranged by size:
3707 971 745 386 117 18
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2098 2132 4135 4169 1903
Size: 34 161 34 2003 34 3678
Fragments arranged by size:
3678 2003 161 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3836 5046 3836
Size: 1210 4734
BclI T'GATC_A
Cuts at: 1548 2460 2643 1548
Size: 912 183 4849
Fragments arranged by size:
4849 912 183
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4555 136
Size: 107 122 71 631 605 2883 1525
Fragments arranged by size:
2883 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2783 5939 2783
Size: 3156 2788
BlpI GC'TnA_GC
Cuts at: 3361 3361
Size: 5944
Bme1580I G_kGCm'C
Cuts at: 1790 2268 2846 4005 5161 1790
Size: 478 578 1159 1156 2573
Fragments arranged by size:
2573 1159 1156 578 478
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5944
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3675 4633 442
Size: 416 53 83 399 24 2258 958 1753
Fragments arranged by size:
2258 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5944
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2647 2678 3052 4604 1239
Size: 1408 31 374 1552 2579
Fragments arranged by size:
2579 1552 1408 374 31
Bpu10I CC'TnA_GC
Cuts at: 2738 2738
Size: 5944
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2804 4069 4934 5232 2804
Size: 1265 865 298 3516
Fragments arranged by size:
3516 1265 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2363 2561 4607 882
Size: 617 864 198 2046 2219
Fragments arranged by size:
2219 2046 864 617 198
BsaAI yAC'GTr
Cuts at: 493 1204 3106 3735 493
Size: 711 1902 629 2702
Fragments arranged by size:
2702 1902 711 629
BsaBI GATnn'nnATC
Cuts at: 5938 5938
Size: 5944
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2073 3751 4133 4768
Size: 53 83 186 936 540 1678 382 635
Cuts at: 4768 275
Size: 1451
Fragments arranged by size:
1678 1451 936 635 540 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4377 4775 5049 5196 5607 4377
Size: 398 274 147 411 4714
Fragments arranged by size:
4714 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3008 3038 3205 3235 1178
Size: 30 165 30 1605 30 167 30 3887
Fragments arranged by size:
3887 1605 167 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5944
BseYI C'CCAG_C
Cuts at: 5147 5643 5147
Size: 496 5448
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5371
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3394 4155 5183 1540
Size: 275 186 1393 761 1028 2301
Fragments arranged by size:
2301 1393 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2811 4005 4090 5161 2811
Size: 1194 85 1071 3594
Fragments arranged by size:
3594 1194 1071 85
BsmI GAATG_Cn'
Cuts at: 3455 3548 3455
Size: 93 5851
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2268 2811 2846 2993 3263 4005 4090
Size: 399 478 543 35 147 270 742 85
Cuts at: 4090 5161 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 543 478 399 270 147
85 35
BspEI T'CCGG_A
Cuts at: 5607 5607
Size: 5944
BspHI T'CATG_A
Cuts at: 3833 5563 3833
Size: 1730 4214
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2579 3024 3299 844
Size: 1735 445 275 3489
Fragments arranged by size:
3489 1735 445 275
BsrBI CCG'CTC
Cuts at: 1394 1823 3831 1394
Size: 429 2008 3507
Fragments arranged by size:
3507 2008 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2161 2175 2604 3064 4439 4613 65
Size: 1183 913 14 429 460 1375 174 1396
Fragments arranged by size:
1396 1375 1183 913 460 429 174 14
BsrFI r'CCGG_y
Cuts at: 1700 1810 4588 4775 1700
Size: 110 2778 187 2869
Fragments arranged by size:
2869 2778 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5944
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5944
BssSI C'ACGA_G
Cuts at: 4004 5016 4004
Size: 1012 4932
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5944
BstBI TT'CG_AA
Cuts at: 4748 4748
Size: 5944
BstEII G'GTnAC_C
Cuts at: 1184 2203 1184
Size: 1019 4925
BstYI r'GATC_y
Cuts at: 1972 2783 3274 3641 4026 4043 5495 5581 5593
Size: 811 491 367 385 17 1452 86 12
Cuts at: 5593 5939 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 811 491 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4453
Fragments arranged by size:
4453 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5205
Fragments arranged by size:
5205 683 56
BtsI GCAGTG_nn'
Cuts at: 2265 2394 3236 3460 4254 4274 2265
Size: 129 842 224 794 20 3935
Fragments arranged by size:
3935 842 794 224 129 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5944
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5909
DraI TTT'AAA
Cuts at: 3248 3248
Size: 5944
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2072 4951 809
Size: 507 756 2879 1802
Fragments arranged by size:
2879 1802 756 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2139 2358 3391
Size: 54 1425 50 275 18 309 219 1033
Cuts at: 3391 4280 5909 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1033 889 309 275 219 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3391 1537
Size: 275 1579 4090
Fragments arranged by size:
4090 1579 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2617 3874 1353
Size: 1264 1257 3423
Fragments arranged by size:
3423 1264 1257
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2776 4531 4905 5051 387
Size: 1064 138 1187 1755 374 146 1280
Fragments arranged by size:
1755 1280 1187 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2647 2678 3052 4007 4604 5391 5659
Size: 393 1015 31 374 955 597 787 268
Cuts at: 5659 1239
Size: 1524
Fragments arranged by size:
1524 1015 955 787 597 393 374 268
31
EcoNI CCTnn'n_nnAGG
Cuts at: 2741 2741
Size: 5944
EcoRI G'AATT_C
Cuts at: 3253 3631 3253
Size: 378 5566
EcoRV GAT'ATC
Cuts at: 3101 3101
Size: 5944
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2815 2815
Size: 5944
FspI TGC'GCA
Cuts at: 3032 4450 3032
Size: 1418 4526
HaeII r_GCGC'y
Cuts at: 1678 2031 5091 1678
Size: 353 3060 2531
Fragments arranged by size:
3060 2531 353
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3085 3117 4585 4617 4659
Size: 32 135 32 1513 32 1468 32 42
Cuts at: 4659 4691 1373
Size: 32 2626
Fragments arranged by size:
2626 1513 1468 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2881 3288 3534 669
Size: 988 281 943 407 246 3079
Fragments arranged by size:
3079 988 943 407 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5944
HpaI GTT'AAC
Cuts at: 2881 3534 2881
Size: 653 5291
KpnI G_GTAC'C
Cuts at: 3269 3269
Size: 5944
MfeI C'AATT_G
Cuts at: 3543 3543
Size: 5944
MluI A'CGCG_T
Cuts at: 5820 5820
Size: 5944
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5058 5242 1305
Size: 3753 184 2007
Fragments arranged by size:
3753 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2141 10
Size: 54 1425 343 309 3813
Fragments arranged by size:
3813 1425 343 309 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2120 2342 2468 2513 2837 3902 4261
Size: 648 954 222 126 45 324 1065 359
Cuts at: 4261 4420 518
Size: 159 2042
Fragments arranged by size:
2042 1065 954 648 359 324 222 159
126 45
MspA1I CmG'CkG
Cuts at: 1606 3360 4037 5185 5430 1606
Size: 1754 677 1148 245 2120
Fragments arranged by size:
2120 1754 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4453
Fragments arranged by size:
4453 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5944
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5944
NotI GC'GGCC_GC
Cuts at: 3391 3391
Size: 5944
NspI r_CATG'y
Cuts at: 1132 1229 2894 3302 4847 1132
Size: 97 1665 408 1545 2229
Fragments arranged by size:
2229 1665 1545 408 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5944
PciI A'CATG_T
Cuts at: 4843 4843
Size: 5944
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5944
PfoI T'CCnGG_A
Cuts at: 2898 3012 2898
Size: 114 5830
PmeI GTTT'AAAC
Cuts at: 3248 3248
Size: 5944
PmlI CAC'GTG
Cuts at: 3106 3106
Size: 5944
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4013 4045 5526 5558 4013
Size: 32 1481 32 4399
Fragments arranged by size:
4399 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5944
PsiI TTA'TAA
Cuts at: 3514 3514
Size: 5944
PstI C_TGCA'G
Cuts at: 830 2199 3296 830
Size: 1369 1097 3478
Fragments arranged by size:
3478 1369 1097
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5944
SalI G'TCGA_C
Cuts at: 1936 3286 1936
Size: 1350 4594
SbfI CC_TGCA'GG
Cuts at: 3296 3296
Size: 5944
ScaI AGT'ACT
Cuts at: 4192 5904 4192
Size: 1712 4232
SfcI C'TryA_G
Cuts at: 826 1045 2195 3292 4427 5108 5299 826
Size: 219 1150 1097 1135 681 191 1471
Fragments arranged by size:
1471 1150 1135 1097 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5944
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5944
SmaI CCC'GGG
Cuts at: 1872 2017 2456 3271 1872
Size: 145 439 815 4545
Fragments arranged by size:
4545 815 439 145
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2819 4048 4949 5211 820
Size: 197 926 876 1229 901 262 1553
Fragments arranged by size:
1553 1229 926 901 876 262 197
SnaBI TAC'GTA
Cuts at: 493 3735 493
Size: 3242 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5944
SphI G_CATG'C
Cuts at: 1132 3302 1132
Size: 2170 3774
SspI AAT'ATT
Cuts at: 5 52 3868 5
Size: 47 3816 2081
Fragments arranged by size:
3816 2081 47
StuI AGG'CCT
Cuts at: 1517 2490 3050 1517
Size: 973 560 4411
Fragments arranged by size:
4411 973 560
StyI C'CwwG_G
Cuts at: 513 1065 2004 3383 513
Size: 552 939 1379 3074
Fragments arranged by size:
3074 1379 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2059 2089 2549 3971 3988 4141 4326
Size: 1255 479 30 460 1422 17 153 185
Cuts at: 4326 4771 325
Size: 445 1498
Fragments arranged by size:
1498 1422 1255 479 460 445 185 153
30 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3224 4190 5902
Size: 274 80 33 51 1443 1247 966 1712
Cuts at: 5902 96
Size: 138
Fragments arranged by size:
1712 1443 1247 966 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2081 2113 3008 3040 3090 3122 5904 5936 2081
Size: 32 895 32 50 32 2782 32 2089
Fragments arranged by size:
2782 2089 895 50 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5944
XbaI T'CTAG_A
Cuts at: 3280 3280
Size: 5944
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5944
XmaI C'CCGG_G
Cuts at: 1870 2015 2454 3269 1870
Size: 145 439 815 4545
Fragments arranged by size:
4545 815 439 145
XmnI GAAnn'nnTTC
Cuts at: 4073 4073
Size: 5944
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2074 3752 276
Size: 53 83 186 936 540 1678 2468
Fragments arranged by size:
2468 1678 936 540 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoO109I FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI