Restriction map for
pFN21AE1884
(Circular) (Six-base) MAPSORT of: pFN21AE1884.seq Check: 5479 from: 1 to: 5935
pFN21AE1884 5935 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3745 278
Size: 53 83 186 936 2209 2468
Fragments arranged by size:
2468 2209 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2328 3278 1937
Size: 391 950 4594
Fragments arranged by size:
4594 950 391
Acc65I G'GTAC_C
Cuts at: 3256 3256
Size: 5935
AclI AA'CG_TT
Cuts at: 1563 4062 4435 1563
Size: 2499 373 3063
Fragments arranged by size:
3063 2499 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2021 2106 2750 2900 3998 5382 5650 1632
Size: 389 85 644 150 1098 1384 268 1917
Fragments arranged by size:
1917 1384 1098 644 389 268 150 85
AflII C'TTAA_G
Cuts at: 820 1017 2887 820
Size: 197 1870 3868
Fragments arranged by size:
3868 1870 197
AflIII A'CryG_T
Cuts at: 1203 4834 5811 1203
Size: 3631 977 1327
Fragments arranged by size:
3631 1327 977
AgeI A'CCGG_T
Cuts at: 4766 4766
Size: 5935
AhdI GACnn_n'nnGTC
Cuts at: 4664 4664
Size: 5935
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5935
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5935
AlwNI CAG_nnn'CTG
Cuts at: 2150 2354 3678 5250 2150
Size: 204 1324 1572 2835
Fragments arranged by size:
2835 1572 1324 204
ApaLI G'TGCA_C
Cuts at: 3992 5148 3992
Size: 1156 4779
ApoI r'AATT_y
Cuts at: 1493 2624 2712 2756 3177 3244 3470 3622 5731
Size: 1131 88 44 421 67 226 152 2109
Cuts at: 5731 1493
Size: 1697
Fragments arranged by size:
2109 1697 1131 421 226 152 88 67
44
AseI AT'TA_AT
Cuts at: 160 1032 4489 160
Size: 872 3457 1606
Fragments arranged by size:
3457 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5935
AvaI C'yCGr_G
Cuts at: 1870 1943 2425 2844 3260 1870
Size: 73 482 419 416 4545
Fragments arranged by size:
4545 482 419 416 73
BamHI G'GATC_C
Cuts at: 3265 3632 3265
Size: 367 5568
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3256 4711 618
Size: 325 217 627 1469 1455 1842
Fragments arranged by size:
1842 1469 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2508 2851 3254 1391
Size: 1117 343 403 4072
Fragments arranged by size:
4072 1117 403 343
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4964
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4126 4160 1903
Size: 34 2189 34 3678
Fragments arranged by size:
3678 2189 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3827 5037 3827
Size: 1210 4725
BclI T'GATC_A
Cuts at: 1548 2536 1548
Size: 988 4947
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4546 136
Size: 107 122 71 631 605 2874 1525
Fragments arranged by size:
2874 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2608 5930 2608
Size: 3322 2613
BlpI GC'TnA_GC
Cuts at: 3352 3352
Size: 5935
Bme1580I G_kGCm'C
Cuts at: 1790 3996 5152 1790
Size: 2206 1156 2573
Fragments arranged by size:
2573 2206 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5935
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3154 3666 4624
Size: 416 53 83 399 24 1737 512 958
Cuts at: 4624 442
Size: 1753
Fragments arranged by size:
1753 1737 958 512 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5935
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2599 4595 1239
Size: 1360 1996 2579
Fragments arranged by size:
2579 1996 1360
Bpu10I CC'TnA_GC
Cuts at: 2154 2154
Size: 5935
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2031 4060 4925 5223 2031
Size: 2029 865 298 2743
Fragments arranged by size:
2743 2029 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4598 882
Size: 617 3099 2219
Fragments arranged by size:
3099 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3726 493
Size: 711 2522 2702
Fragments arranged by size:
2702 2522 711
BsaBI GATnn'nnATC
Cuts at: 5929 5929
Size: 5935
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3742 4124 4759 275
Size: 53 83 186 936 2209 382 635 1451
Fragments arranged by size:
2209 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4368 4766 5040 5187 5598 4368
Size: 398 274 147 411 4705
Fragments arranged by size:
4705 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2727 2757 1178
Size: 30 165 30 1324 30 4356
Fragments arranged by size:
4356 1324 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3129 1186
Size: 1943 3992
BseYI C'CCAG_C
Cuts at: 2143 5138 5634 2143
Size: 2995 496 2444
Fragments arranged by size:
2995 2444 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5362
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3385 4146 5174 1540
Size: 275 186 1384 761 1028 2301
Fragments arranged by size:
2301 1384 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2836 3996 4081 5152 2836
Size: 1160 85 1071 3619
Fragments arranged by size:
3619 1160 1071 85
BsmI GAATG_Cn'
Cuts at: 3135 3446 3539 3135
Size: 311 93 5531
Fragments arranged by size:
5531 311 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2508 2836 2851 3254 3996 4081 5152
Size: 399 718 328 15 403 742 85 1071
Cuts at: 5152 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 718 403 399 328 85
15
BspEI T'CCGG_A
Cuts at: 5598 5598
Size: 5935
BspHI T'CATG_A
Cuts at: 3824 5554 3824
Size: 1730 4205
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3290 844
Size: 2446 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3822 1394
Size: 429 1999 3507
Fragments arranged by size:
3507 1999 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4430 4604 65
Size: 1183 3182 174 1396
Fragments arranged by size:
3182 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4579 4766 1700
Size: 110 2769 187 2869
Fragments arranged by size:
2869 2769 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5935
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5935
BssSI C'ACGA_G
Cuts at: 3995 5007 3995
Size: 1012 4923
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2831 794
Size: 2037 3898
BstBI TT'CG_AA
Cuts at: 4739 4739
Size: 5935
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5935
BstXI CCAn_nnnn'nTGG
Cuts at: 2581 2581
Size: 5935
BstYI r'GATC_y
Cuts at: 1972 2608 3265 3632 4017 4034 5486 5572 5584
Size: 636 657 367 385 17 1452 86 12
Cuts at: 5584 5930 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 657 636 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4444
Fragments arranged by size:
4444 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5196
Fragments arranged by size:
5196 683 56
BtsI GCAGTG_nn'
Cuts at: 2691 3067 3155 3451 4245 4265 2691
Size: 376 88 296 794 20 4361
Fragments arranged by size:
4361 794 376 296 88 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5935
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5900
DraI TTT'AAA
Cuts at: 2311 3239 2311
Size: 928 5007
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4942 809
Size: 507 3626 1802
Fragments arranged by size:
3626 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3382 4271 5900
Size: 54 1425 50 275 18 1552 889 1629
Cuts at: 5900 8
Size: 43
Fragments arranged by size:
1629 1552 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3382 1537
Size: 275 1570 4090
Fragments arranged by size:
4090 1570 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3085 3865 1353
Size: 1732 780 3423
Fragments arranged by size:
3423 1732 780
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4522 4896 5042 387
Size: 1064 138 2933 374 146 1280
Fragments arranged by size:
2933 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2021 2106 2599 2750 2900 3998 4595
Size: 393 389 85 493 151 150 1098 597
Cuts at: 4595 5382 5650 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1098 787 597 493 393 389 268
151 150 85
EcoRI G'AATT_C
Cuts at: 3244 3622 3244
Size: 378 5557
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2375 2375
Size: 5935
FspI TGC'GCA
Cuts at: 4441 4441
Size: 5935
HaeII r_GCGC'y
Cuts at: 1678 5082 1678
Size: 3404 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2356 2380 2388 2412 4576
Size: 32 135 32 784 24 8 24 2164
Cuts at: 4576 4608 4650 4682 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2164 784 135 42 32 32 32
32 24 24 8
HincII GTy'rAC
Cuts at: 669 1657 1938 3279 3525 669
Size: 988 281 1341 246 3079
Fragments arranged by size:
3079 1341 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2049 748
Size: 1301 4634
HpaI GTT'AAC
Cuts at: 3525 3525
Size: 5935
KpnI G_GTAC'C
Cuts at: 3260 3260
Size: 5935
MfeI C'AATT_G
Cuts at: 3534 3534
Size: 5935
MluI A'CGCG_T
Cuts at: 5811 5811
Size: 5935
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5049 5233 1305
Size: 3744 184 2007
Fragments arranged by size:
3744 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4113
Fragments arranged by size:
4113 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2532 3893 4252 4411 518
Size: 648 1366 1361 359 159 2042
Fragments arranged by size:
2042 1366 1361 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2521 3351 4028 5176 5421 1606
Size: 915 830 677 1148 245 2120
Fragments arranged by size:
2120 1148 915 830 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4444
Fragments arranged by size:
4444 939 552
NdeI CA'TA_TG
Cuts at: 387 2529 3028 387
Size: 2142 499 3294
Fragments arranged by size:
3294 2142 499
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5935
NotI GC'GGCC_GC
Cuts at: 3382 3382
Size: 5935
NspI r_CATG'y
Cuts at: 1132 1229 3293 4838 1132
Size: 97 2064 1545 2229
Fragments arranged by size:
2229 2064 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5935
PciI A'CATG_T
Cuts at: 4834 4834
Size: 5935
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5935
PmeI GTTT'AAAC
Cuts at: 3239 3239
Size: 5935
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4004 4036 5517 5549 4004
Size: 32 1481 32 4390
Fragments arranged by size:
4390 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5935
PsiI TTA'TAA
Cuts at: 3505 3505
Size: 5935
PspXI vC'TCGA_Gb
Cuts at: 2844 2844
Size: 5935
PstI C_TGCA'G
Cuts at: 830 3287 830
Size: 2457 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5935
PvuII CAG'CTG
Cuts at: 2521 2521
Size: 5935
SalI G'TCGA_C
Cuts at: 1936 3277 1936
Size: 1341 4594
SbfI CC_TGCA'GG
Cuts at: 3287 3287
Size: 5935
ScaI AGT'ACT
Cuts at: 4183 5895 4183
Size: 1712 4223
SfcI C'TryA_G
Cuts at: 826 1045 2954 3283 4418 5099 5290 826
Size: 219 1909 329 1135 681 191 1471
Fragments arranged by size:
1909 1471 1135 681 329 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5935
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5935
SmaI CCC'GGG
Cuts at: 1872 2427 3262 1872
Size: 555 835 4545
Fragments arranged by size:
4545 835 555
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2046 2844 2887 4039 4940 5202
Size: 197 926 103 798 43 1152 901 262
Cuts at: 5202 820
Size: 1553
Fragments arranged by size:
1553 1152 926 901 798 262 197 103
43
SnaBI TAC'GTA
Cuts at: 493 3726 493
Size: 3233 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5935
SphI G_CATG'C
Cuts at: 1132 3293 1132
Size: 2161 3774
SspI AAT'ATT
Cuts at: 5 52 3859 5
Size: 47 3807 2081
Fragments arranged by size:
3807 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5935
StyI C'CwwG_G
Cuts at: 513 1065 2004 3374 513
Size: 552 939 1370 3074
Fragments arranged by size:
3074 1370 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3962 3979 4132 4317 4762 325
Size: 1255 2382 17 153 185 445 1498
Fragments arranged by size:
2382 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2650 4181 5893
Size: 274 80 33 51 1443 673 1531 1712
Cuts at: 5893 96
Size: 138
Fragments arranged by size:
1712 1531 1443 673 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5895 5927 5895
Size: 32 5903
Tth111I GACn'n_nGTC
Cuts at: 1316 2405 3160 1316
Size: 1089 755 4091
Fragments arranged by size:
4091 1089 755
XbaI T'CTAG_A
Cuts at: 3271 3271
Size: 5935
XhoI C'TCGA_G
Cuts at: 1943 2844 1943
Size: 901 5034
XmaI C'CCGG_G
Cuts at: 1870 2425 3260 1870
Size: 555 835 4545
Fragments arranged by size:
4545 835 555
XmnI GAAnn'nnTTC
Cuts at: 2133 4064 2133
Size: 1931 4004
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3743 276
Size: 53 83 186 936 2209 2468
Fragments arranged by size:
2468 2209 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI