Restriction map for
pFN21AE1883
(Circular) (Six-base) MAPSORT of: pFN21AE1883.seq Check: 4384 from: 1 to: 5932
pFN21AE1883 5932 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2899 2899
Size: 5932
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3742 278
Size: 53 83 186 936 2206 2468
Fragments arranged by size:
2468 2206 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2729 3156 3275 1937
Size: 792 427 119 4594
Fragments arranged by size:
4594 792 427 119
Acc65I G'GTAC_C
Cuts at: 3253 3253
Size: 5932
AclI AA'CG_TT
Cuts at: 1563 4059 4432 1563
Size: 2496 373 3063
Fragments arranged by size:
3063 2496 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2219 3084 3995 5379 5647 1632
Size: 587 865 911 1384 268 1917
Fragments arranged by size:
1917 1384 911 865 587 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5735
AflIII A'CryG_T
Cuts at: 1203 2985 4831 5808 1203
Size: 1782 1846 977 1327
Fragments arranged by size:
1846 1782 1327 977
AgeI A'CCGG_T
Cuts at: 4763 4763
Size: 5932
AhdI GACnn_n'nnGTC
Cuts at: 2017 4661 2017
Size: 2644 3288
AleI CACnn'nnGTG
Cuts at: 1166 2660 1166
Size: 1494 4438
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2813 2909 1184
Size: 1629 96 4207
Fragments arranged by size:
4207 1629 96
AlwNI CAG_nnn'CTG
Cuts at: 2483 3200 3675 5247 2483
Size: 717 475 1572 3168
Fragments arranged by size:
3168 1572 717 475
ApaLI G'TGCA_C
Cuts at: 3989 5145 3989
Size: 1156 4776
ApoI r'AATT_y
Cuts at: 1493 3007 3241 3467 3619 5728 1493
Size: 1514 234 226 152 2109 1697
Fragments arranged by size:
2109 1697 1514 234 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4486 160
Size: 872 3454 1606
Fragments arranged by size:
3454 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5932
AvaI C'yCGr_G
Cuts at: 1870 1943 2120 2636 3257 1870
Size: 73 177 516 621 4545
Fragments arranged by size:
4545 621 516 177 73
BamHI G'GATC_C
Cuts at: 2145 2473 3262 3629 2145
Size: 328 789 367 4448
Fragments arranged by size:
4448 789 367 328
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2893 3253 4708 618
Size: 325 217 627 1106 360 1455 1842
Fragments arranged by size:
1842 1455 1106 627 360 325 217
BanII G_rGCy'C
Cuts at: 1391 2825 3251 1391
Size: 1434 426 4072
Fragments arranged by size:
4072 1434 426
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4961
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3117 3151 4123 4157 1903
Size: 34 1180 34 972 34 3678
Fragments arranged by size:
3678 1180 972 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3824 5034 3824
Size: 1210 4722
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5932
BfrBI ATG'CAT
Cuts at: 2504 2504
Size: 5932
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4543 136
Size: 107 122 71 631 605 2871 1525
Fragments arranged by size:
2871 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2554 5927 2554
Size: 3373 2559
BlpI GC'TnA_GC
Cuts at: 3349 3349
Size: 5932
Bme1580I G_kGCm'C
Cuts at: 1790 2740 2898 3993 5149 1790
Size: 950 158 1095 1156 2573
Fragments arranged by size:
2573 1156 1095 950 158
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5932
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3663 4621 442
Size: 416 53 83 399 24 2246 958 1753
Fragments arranged by size:
2246 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5932
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2844 2844
Size: 5932
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4592 1239
Size: 3353 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4057 4922 5220 4057
Size: 865 298 4769
Fragments arranged by size:
4769 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4595 882
Size: 617 3096 2219
Fragments arranged by size:
3096 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3723 493
Size: 711 2519 2702
Fragments arranged by size:
2702 2519 711
BsaBI GATnn'nnATC
Cuts at: 2885 5926 2885
Size: 3041 2891
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3739 4121 4756 275
Size: 53 83 186 936 2206 382 635 1451
Fragments arranged by size:
2206 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2454 2718 4365 4763 5037 5184 5595 2454
Size: 264 1647 398 274 147 411 2791
Fragments arranged by size:
2791 1647 411 398 274 264 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5707
Fragments arranged by size:
5707 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5932
BseYI C'CCAG_C
Cuts at: 2239 2703 5135 5631 2239
Size: 464 2432 496 2540
Fragments arranged by size:
2540 2432 496 464
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2927 1163
Size: 573 1191 4168
Fragments arranged by size:
4168 1191 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3382 4143 5171 1540
Size: 275 186 1381 761 1028 2301
Fragments arranged by size:
2301 1381 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2574 2825 3993 4078 5149 2574
Size: 251 1168 85 1071 3357
Fragments arranged by size:
3357 1168 1071 251 85
BsmI GAATG_Cn'
Cuts at: 2398 3443 3536 2398
Size: 1045 93 4794
Fragments arranged by size:
4794 1045 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2344 2344
Size: 5932
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2574 2740 2825 2898 3251 3993 4078
Size: 399 784 166 85 73 353 742 85
Cuts at: 4078 5149 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 784 742 399 353 166 85
85 73
BspEI T'CCGG_A
Cuts at: 2454 5595 2454
Size: 3141 2791
BspHI T'CATG_A
Cuts at: 2182 3821 5551 2182
Size: 1639 1730 2563
Fragments arranged by size:
2563 1730 1639
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2007 2899 3287 844
Size: 1163 892 388 3489
Fragments arranged by size:
3489 1163 892 388
BsrBI CCG'CTC
Cuts at: 1394 1823 3819 1394
Size: 429 1996 3507
Fragments arranged by size:
3507 1996 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3123 4427 4601 65
Size: 1183 1875 1304 174 1396
Fragments arranged by size:
1875 1396 1304 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4576 4763 1700
Size: 110 2766 187 2869
Fragments arranged by size:
2869 2766 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5932
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5932
BssSI C'ACGA_G
Cuts at: 3992 5004 3992
Size: 1012 4920
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3011 3113 794
Size: 2217 102 3613
Fragments arranged by size:
3613 2217 102
BstBI TT'CG_AA
Cuts at: 4736 4736
Size: 5932
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5932
BstYI r'GATC_y
Cuts at: 1972 2145 2473 2554 3262 3629 4014 4031 5483
Size: 173 328 81 708 367 385 17 1452
Cuts at: 5483 5569 5581 5927 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 708 385 367 346 328 173
86 81 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2206 2274 2779 513
Size: 552 1141 68 505 3666
Fragments arranged by size:
3666 1141 552 505 68
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3120 487
Size: 683 56 1894 3299
Fragments arranged by size:
3299 1894 683 56
BtsI GCAGTG_nn'
Cuts at: 2604 2807 3448 4242 4262 2604
Size: 203 641 794 20 4274
Fragments arranged by size:
4274 794 641 203 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5932
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5897
DraI TTT'AAA
Cuts at: 3236 3236
Size: 5932
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4939 809
Size: 507 3623 1802
Fragments arranged by size:
3623 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3379 4268 5897
Size: 54 1425 50 275 18 1549 889 1629
Cuts at: 5897 8
Size: 43
Fragments arranged by size:
1629 1549 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3379 1537
Size: 275 1567 4090
Fragments arranged by size:
4090 1567 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2066 3862 1353
Size: 713 1796 3423
Fragments arranged by size:
3423 1796 713
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2261 4519 4893 5039 387
Size: 1064 138 672 2258 374 146 1280
Fragments arranged by size:
2258 1280 1064 672 374 146 138
EcoICRI GAG'CTC
Cuts at: 2823 2823
Size: 5932
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2219 3084 3995 4592 5379 5647 1239
Size: 393 587 865 911 597 787 268 1524
Fragments arranged by size:
1524 911 865 787 597 587 393 268
EcoRI G'AATT_C
Cuts at: 3241 3619 3241
Size: 378 5554
FspI TGC'GCA
Cuts at: 4438 4438
Size: 5932
HaeII r_GCGC'y
Cuts at: 1678 5079 1678
Size: 3401 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2003 2035 2465 2497 2524
Size: 32 135 32 431 32 430 32 27
Cuts at: 2524 2556 2571 2603 2663 2695 4573 4605 4647
Size: 32 15 32 60 32 1878 32 42
Cuts at: 4647 4679 1373
Size: 32 2626
Fragments arranged by size:
2626 1878 431 430 135 60 42 32
32 32 32 32 32 32 32 32
27 15
HincII GTy'rAC
Cuts at: 669 1657 1938 2730 3276 3522 669
Size: 988 281 792 546 246 3079
Fragments arranged by size:
3079 988 792 546 281 246
HindIII A'AGCT_T
Cuts at: 748 3096 748
Size: 2348 3584
HpaI GTT'AAC
Cuts at: 3522 3522
Size: 5932
KpnI G_GTAC'C
Cuts at: 3257 3257
Size: 5932
MfeI C'AATT_G
Cuts at: 3531 3531
Size: 5932
MluI A'CGCG_T
Cuts at: 5808 5808
Size: 5932
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2160 2656 3205 5046 5230 1305
Size: 855 496 549 1841 184 2007
Fragments arranged by size:
2007 1841 855 549 496 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4110
Fragments arranged by size:
4110 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2279 2660 2795 2891 3890 4249 4408
Size: 648 1113 381 135 96 999 359 159
Cuts at: 4408 518
Size: 2042
Fragments arranged by size:
2042 1113 999 648 381 359 159 135
96
MspA1I CmG'CkG
Cuts at: 1606 2239 2833 2877 2933 2962 3348 4025 5173
Size: 633 594 44 56 29 386 677 1148
Cuts at: 5173 5418 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 633 594 386 245 56
44 29
NcoI C'CATG_G
Cuts at: 513 1065 2206 2274 513
Size: 552 1141 68 4171
Fragments arranged by size:
4171 1141 552 68
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5932
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5932
NotI GC'GGCC_GC
Cuts at: 3379 3379
Size: 5932
NsiI A_TGCA'T
Cuts at: 2506 2506
Size: 5932
NspI r_CATG'y
Cuts at: 1132 1229 2800 2989 3290 4835 1132
Size: 97 1571 189 301 1545 2229
Fragments arranged by size:
2229 1571 1545 301 189 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5932
PciI A'CATG_T
Cuts at: 2985 4831 2985
Size: 1846 4086
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2046 1108
Size: 938 4994
PmeI GTTT'AAAC
Cuts at: 3236 3236
Size: 5932
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4001 4033 5514 5546 4001
Size: 32 1481 32 4387
Fragments arranged by size:
4387 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5932
PsiI TTA'TAA
Cuts at: 3502 3502
Size: 5932
PstI C_TGCA'G
Cuts at: 830 2018 3284 830
Size: 1188 1266 3478
Fragments arranged by size:
3478 1266 1188
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5932
PvuII CAG'CTG
Cuts at: 2239 2877 2933 2239
Size: 638 56 5238
Fragments arranged by size:
5238 638 56
SacI G_AGCT'C
Cuts at: 2825 2825
Size: 5932
SalI G'TCGA_C
Cuts at: 1936 2728 3274 1936
Size: 792 546 4594
Fragments arranged by size:
4594 792 546
SbfI CC_TGCA'GG
Cuts at: 3284 3284
Size: 5932
ScaI AGT'ACT
Cuts at: 4180 5892 4180
Size: 1712 4220
SfcI C'TryA_G
Cuts at: 826 1045 2014 2934 3192 3280 4415 5096 5287
Size: 219 969 920 258 88 1135 681 191
Cuts at: 5287 826
Size: 1471
Fragments arranged by size:
1471 1135 969 920 681 258 219 191
88
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5932
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5932
SmaI CCC'GGG
Cuts at: 1872 3259 1872
Size: 1387 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2120 4036 4937 5199 820
Size: 197 926 177 1916 901 262 1553
Fragments arranged by size:
1916 1553 926 901 262 197 177
SnaBI TAC'GTA
Cuts at: 493 3723 493
Size: 3230 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5932
SphI G_CATG'C
Cuts at: 1132 2800 3290 1132
Size: 1668 490 3774
Fragments arranged by size:
3774 1668 490
SspI AAT'ATT
Cuts at: 5 52 2313 3856 5
Size: 47 2261 1543 2081
Fragments arranged by size:
2261 2081 1543 47
StuI AGG'CCT
Cuts at: 1517 2179 1517
Size: 662 5270
StyI C'CwwG_G
Cuts at: 513 1065 2025 2206 2274 3371 513
Size: 552 960 181 68 1097 3074
Fragments arranged by size:
3074 1097 960 552 181 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2209 3959 3976 4129 4314 4759 325
Size: 1255 629 1750 17 153 185 445 1498
Fragments arranged by size:
1750 1498 1255 629 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4178 5890 96
Size: 274 80 33 51 1443 2201 1712 138
Fragments arranged by size:
2201 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5892 5924 5892
Size: 32 5900
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5932
XbaI T'CTAG_A
Cuts at: 3268 3268
Size: 5932
XhoI C'TCGA_G
Cuts at: 1943 2120 1943
Size: 177 5755
XmaI C'CCGG_G
Cuts at: 1870 3257 1870
Size: 1387 4545
XmnI GAAnn'nnTTC
Cuts at: 4061 4061
Size: 5932
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3740 276
Size: 53 83 186 936 2206 2468
Fragments arranged by size:
2468 2206 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI