Restriction map for
pFN21AE1877
(Circular) (Six-base) MAPSORT of: pFN21AE1877.seq Check: 6405 from: 1 to: 5899
pFN21AE1877 5899 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2095 2516 2825 2999 2095
Size: 421 309 174 4995
Fragments arranged by size:
4995 421 309 174
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3709 278
Size: 53 83 186 936 2173 2468
Fragments arranged by size:
2468 2173 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3180 3242 1937
Size: 1243 62 4594
Fragments arranged by size:
4594 1243 62
Acc65I G'GTAC_C
Cuts at: 2924 3220 2924
Size: 296 5603
AclI AA'CG_TT
Cuts at: 1563 4026 4399 1563
Size: 2463 373 3063
Fragments arranged by size:
3063 2463 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3062 3962 5346 5614 1632
Size: 1430 900 1384 268 1917
Fragments arranged by size:
1917 1430 1384 900 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5702
AflIII A'CryG_T
Cuts at: 1203 2877 4798 5775 1203
Size: 1674 1921 977 1327
Fragments arranged by size:
1921 1674 1327 977
AgeI A'CCGG_T
Cuts at: 4730 4730
Size: 5899
AhdI GACnn_n'nnGTC
Cuts at: 2614 4628 2614
Size: 2014 3885
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5899
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5899
AlwNI CAG_nnn'CTG
Cuts at: 3642 5214 3642
Size: 1572 4327
ApaI G_GGCC'C
Cuts at: 2196 2468 3140 2196
Size: 272 672 4955
Fragments arranged by size:
4955 672 272
ApaLI G'TGCA_C
Cuts at: 3956 5112 3956
Size: 1156 4743
ApoI r'AATT_y
Cuts at: 1493 3208 3434 3586 5695 1493
Size: 1715 226 152 2109 1697
Fragments arranged by size:
2109 1715 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2234 4453 160
Size: 872 1202 2219 1606
Fragments arranged by size:
2219 1606 1202 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5899
AvaI C'yCGr_G
Cuts at: 1870 1943 3224 1870
Size: 73 1281 4545
Fragments arranged by size:
4545 1281 73
BamHI G'GATC_C
Cuts at: 3229 3596 3229
Size: 367 5532
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2351 2417 2924 3220 4675
Size: 325 217 627 564 66 507 296 1455
Cuts at: 4675 618
Size: 1842
Fragments arranged by size:
1842 1455 627 564 507 325 296 217
66
BanII G_rGCy'C
Cuts at: 1391 2196 2387 2468 2574 3074 3140 3218 1391
Size: 805 191 81 106 500 66 78 4072
Fragments arranged by size:
4072 805 500 191 106 81 78 66
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4928
BbvCI CC'TCA_GC
Cuts at: 2487 2487
Size: 5899
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4090 4124 1903
Size: 34 50 34 2069 34 3678
Fragments arranged by size:
3678 2069 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3791 5001 3791
Size: 1210 4689
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5899
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4510 136
Size: 107 122 71 631 605 2838 1525
Fragments arranged by size:
2838 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5894 5894
Size: 5899
BlpI GC'TnA_GC
Cuts at: 3316 3316
Size: 5899
Bme1580I G_kGCm'C
Cuts at: 1790 2196 2468 3140 3960 5116 1790
Size: 406 272 672 820 1156 2573
Fragments arranged by size:
2573 1156 820 672 406 272
BmgBI CAC'GTC
Cuts at: 1318 2698 1318
Size: 1380 4519
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3630 4588 442
Size: 416 53 83 399 24 2213 958 1753
Fragments arranged by size:
2213 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5899
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3088 3088
Size: 5899
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2163 2430 4559 1239
Size: 924 267 2129 2579
Fragments arranged by size:
2579 2129 924 267
Bpu10I CC'TnA_GC
Cuts at: 2487 2487
Size: 5899
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2658 4024 4889 5187 2658
Size: 1366 865 298 3370
Fragments arranged by size:
3370 1366 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2738 3052 4562 882
Size: 617 1239 314 1510 2219
Fragments arranged by size:
2219 1510 1239 617 314
BsaAI yAC'GTr
Cuts at: 493 1204 2395 3690 493
Size: 711 1191 1295 2702
Fragments arranged by size:
2702 1295 1191 711
BsaBI GATnn'nnATC
Cuts at: 5893 5893
Size: 5899
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3706 4088 4723 275
Size: 53 83 186 936 2173 382 635 1451
Fragments arranged by size:
2173 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2373 4332 4730 5004 5151 5562 2373
Size: 1959 398 274 147 411 2710
Fragments arranged by size:
2710 1959 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2390 2420 2598 2628 1178
Size: 30 165 30 987 30 178 30 4449
Fragments arranged by size:
4449 987 178 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2226 1186
Size: 1040 4859
BseYI C'CCAG_C
Cuts at: 2065 2338 3091 5102 5598 2065
Size: 273 753 2011 496 2366
Fragments arranged by size:
2366 2011 753 496 273
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2144 2149 1163
Size: 573 408 5 4913
Fragments arranged by size:
4913 573 408 5
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3349 4110 5138 1540
Size: 275 186 1348 761 1028 2301
Fragments arranged by size:
2301 1348 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2574 3960 4045 5116 2574
Size: 1386 85 1071 3357
Fragments arranged by size:
3357 1386 1071 85
BsmI GAATG_Cn'
Cuts at: 3410 3503 3410
Size: 93 5806
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2196 2387 2468 2574 3074 3140 3218
Size: 399 406 191 81 106 500 66 78
Cuts at: 3218 3960 4045 5116 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 500 406 399 191 106
85 81 78 66
BspEI T'CCGG_A
Cuts at: 2373 5562 2373
Size: 3189 2710
BspHI T'CATG_A
Cuts at: 3788 5518 3788
Size: 1730 4169
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2095 2516 2825 2999 3254 844
Size: 1251 421 309 174 255 3489
Fragments arranged by size:
3489 1251 421 309 255 174
BsrBI CCG'CTC
Cuts at: 1394 1823 3786 1394
Size: 429 1963 3507
Fragments arranged by size:
3507 1963 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4394 4568 65
Size: 1183 3146 174 1396
Fragments arranged by size:
3146 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2348 4543 4730 1700
Size: 110 538 2195 187 2869
Fragments arranged by size:
2869 2195 538 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5899
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5899
BssSI C'ACGA_G
Cuts at: 3959 4971 3959
Size: 1012 4887
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5899
BstBI TT'CG_AA
Cuts at: 4703 4703
Size: 5899
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5899
BstXI CCAn_nnnn'nTGG
Cuts at: 2022 2022
Size: 5899
BstYI r'GATC_y
Cuts at: 1972 3229 3596 3981 3998 5450 5536 5548 5894
Size: 1257 367 385 17 1452 86 12 346
Cuts at: 5894 1972
Size: 1977
Fragments arranged by size:
1977 1452 1257 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2264 2454 2518 513
Size: 552 1199 190 64 3894
Fragments arranged by size:
3894 1199 552 190 64
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5160
Fragments arranged by size:
5160 683 56
BtsI GCAGTG_nn'
Cuts at: 2161 3415 4209 4229 2161
Size: 1254 794 20 3831
Fragments arranged by size:
3831 1254 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5899
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2748 2783 529
Size: 35 2184 35 3645
Fragments arranged by size:
3645 2184 35 35
DraI TTT'AAA
Cuts at: 3203 3203
Size: 5899
DraIII CAC_nnn'GTG
Cuts at: 2497 2497
Size: 5899
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3044 4906 809
Size: 507 1728 1862 1802
Fragments arranged by size:
1862 1802 1728 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2151 3346 4235
Size: 54 1425 50 275 18 321 1195 889
Cuts at: 4235 5864 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1195 889 321 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3346 1537
Size: 275 1534 4090
Fragments arranged by size:
4090 1534 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2091 2724 3829 1353
Size: 738 633 1105 3423
Fragments arranged by size:
3423 1105 738 633
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4486 4860 5006 387
Size: 1064 138 2897 374 146 1280
Fragments arranged by size:
2897 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2572 2572
Size: 5899
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2163 2430 3062 3962 4559 5346 5614
Size: 393 531 267 632 900 597 787 268
Cuts at: 5614 1239
Size: 1524
Fragments arranged by size:
1524 900 787 632 597 531 393 268
267
EcoO109I rG'GnC_Cy
Cuts at: 2192 2465 2713 3137 2192
Size: 273 248 424 4954
Fragments arranged by size:
4954 424 273 248
EcoRI G'AATT_C
Cuts at: 3208 3586 3208
Size: 378 5521
FspI TGC'GCA
Cuts at: 4405 4405
Size: 5899
HaeII r_GCGC'y
Cuts at: 1678 5046 1678
Size: 3368 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2390 2422 2600 2632 2654
Size: 32 135 32 818 32 178 32 22
Cuts at: 2654 2686 4540 4572 4614 4646 1373
Size: 32 1854 32 42 32 2626
Fragments arranged by size:
2626 1854 818 178 135 42 32 32
32 32 32 32 32 22
HincII GTy'rAC
Cuts at: 669 1657 1938 3243 3489 669
Size: 988 281 1305 246 3079
Fragments arranged by size:
3079 1305 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5899
HpaI GTT'AAC
Cuts at: 3489 3489
Size: 5899
KpnI G_GTAC'C
Cuts at: 2928 3224 2928
Size: 296 5603
MfeI C'AATT_G
Cuts at: 3498 3498
Size: 5899
MluI A'CGCG_T
Cuts at: 5775 5775
Size: 5899
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2844 5013 5197 1305
Size: 1539 2169 184 2007
Fragments arranged by size:
2169 2007 1539 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4077
Fragments arranged by size:
4077 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2973 3857 4216 4375 518
Size: 648 843 964 884 359 159 2042
Fragments arranged by size:
2042 964 884 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2141 2854 3020 3315 3992 5140 5385 1606
Size: 535 713 166 295 677 1148 245 2120
Fragments arranged by size:
2120 1148 713 677 535 295 245 166
NaeI GCC'GGC
Cuts at: 2350 2350
Size: 5899
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5347
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5899
NgoMIV G'CCGG_C
Cuts at: 2348 2348
Size: 5899
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5899
NotI GC'GGCC_GC
Cuts at: 3346 3346
Size: 5899
NspI r_CATG'y
Cuts at: 1132 1229 3257 4802 1132
Size: 97 2028 1545 2229
Fragments arranged by size:
2229 2028 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5899
PciI A'CATG_T
Cuts at: 4798 4798
Size: 5899
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2202 1108
Size: 1094 4805
PfoI T'CCnGG_A
Cuts at: 2882 2882
Size: 5899
PmeI GTTT'AAAC
Cuts at: 3203 3203
Size: 5899
PmlI CAC'GTG
Cuts at: 2395 2395
Size: 5899
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3968 4000 5481 5513 3968
Size: 32 1481 32 4354
Fragments arranged by size:
4354 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2713 2713
Size: 5899
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5899
PsiI TTA'TAA
Cuts at: 3469 3469
Size: 5899
PspOMI G'GGCC_C
Cuts at: 2192 2464 3136 2192
Size: 272 672 4955
Fragments arranged by size:
4955 672 272
PstI C_TGCA'G
Cuts at: 830 2106 3251 830
Size: 1276 1145 3478
Fragments arranged by size:
3478 1276 1145
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5899
PvuII CAG'CTG
Cuts at: 2141 2854 2141
Size: 713 5186
SacI G_AGCT'C
Cuts at: 2574 2574
Size: 5899
SalI G'TCGA_C
Cuts at: 1936 3241 1936
Size: 1305 4594
SanDI GG'GwC_CC
Cuts at: 2713 2713
Size: 5899
SapI GCTCTTCn'nnn_
Cuts at: 2091 2091
Size: 5899
SbfI CC_TGCA'GG
Cuts at: 3251 3251
Size: 5899
ScaI AGT'ACT
Cuts at: 4147 5859 4147
Size: 1712 4187
SexAI A'CCwGG_T
Cuts at: 2802 2858 2802
Size: 56 5843
SfcI C'TryA_G
Cuts at: 826 1045 2102 2620 2849 3247 4382 5063 5254
Size: 219 1057 518 229 398 1135 681 191
Cuts at: 5254 826
Size: 1471
Fragments arranged by size:
1471 1135 1057 681 518 398 229 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5899
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5899
SmaI CCC'GGG
Cuts at: 1872 3226 1872
Size: 1354 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2673 4003 4904 5166 820
Size: 197 926 730 1330 901 262 1553
Fragments arranged by size:
1553 1330 926 901 730 262 197
SnaBI TAC'GTA
Cuts at: 493 3690 493
Size: 3197 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5899
SphI G_CATG'C
Cuts at: 1132 3257 1132
Size: 2125 3774
SspI AAT'ATT
Cuts at: 5 52 3823 5
Size: 47 3771 2081
Fragments arranged by size:
3771 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5899
StyI C'CwwG_G
Cuts at: 513 1065 2640 2766 3141 3338 513
Size: 552 1575 126 375 197 3074
Fragments arranged by size:
3074 1575 552 375 197 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2545 2776 3926 3943 4096 4281 4726
Size: 1255 965 231 1150 17 153 185 445
Cuts at: 4726 325
Size: 1498
Fragments arranged by size:
1498 1255 1150 965 445 231 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4145 5857 96
Size: 274 80 33 51 1443 2168 1712 138
Fragments arranged by size:
2168 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2598 2630 5859 5891 2598
Size: 32 3229 32 2606
Fragments arranged by size:
3229 2606 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5899
XbaI T'CTAG_A
Cuts at: 3235 3235
Size: 5899
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3148 3148
Size: 5899
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5899
XmaI C'CCGG_G
Cuts at: 1870 3224 1870
Size: 1354 4545
XmnI GAAnn'nnTTC
Cuts at: 2552 4028 2552
Size: 1476 4423
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3707 276
Size: 53 83 186 936 2173 2468
Fragments arranged by size:
2468 2173 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PspXI PsrI RsrII
SacII SfiI SfoI SrfI SwaI