Restriction map for
pFN21AE1873
(Circular) (Six-base) MAPSORT of: pFN21AE1873.seq Check: 9841 from: 1 to: 5887
pFN21AE1873 5887 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3697 278
Size: 53 83 186 936 2161 2468
Fragments arranged by size:
2468 2161 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3126 3230 1937
Size: 1189 104 4594
Fragments arranged by size:
4594 1189 104
Acc65I G'GTAC_C
Cuts at: 3208 3208
Size: 5887
AclI AA'CG_TT
Cuts at: 1563 4014 4387 1563
Size: 2451 373 3063
Fragments arranged by size:
3063 2451 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2294 3950 5334 5602 1632
Size: 662 1656 1384 268 1917
Fragments arranged by size:
1917 1656 1384 662 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5690
AflIII A'CryG_T
Cuts at: 1203 2328 4786 5763 1203
Size: 1125 2458 977 1327
Fragments arranged by size:
2458 1327 1125 977
AgeI A'CCGG_T
Cuts at: 4718 4718
Size: 5887
AhdI GACnn_n'nnGTC
Cuts at: 4616 4616
Size: 5887
AleI CACnn'nnGTG
Cuts at: 1166 2078 1166
Size: 912 4975
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5887
AlwNI CAG_nnn'CTG
Cuts at: 2987 3630 5202 2987
Size: 643 1572 3672
Fragments arranged by size:
3672 1572 643
ApaLI G'TGCA_C
Cuts at: 3944 5100 3944
Size: 1156 4731
ApoI r'AATT_y
Cuts at: 1493 2945 3196 3422 3574 5683 1493
Size: 1452 251 226 152 2109 1697
Fragments arranged by size:
2109 1697 1452 251 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4441 160
Size: 872 3409 1606
Fragments arranged by size:
3409 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5887
AvaI C'yCGr_G
Cuts at: 1870 1943 2218 3212 1870
Size: 73 275 994 4545
Fragments arranged by size:
4545 994 275 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3147 3180 3147
Size: 33 5854
BamHI G'GATC_C
Cuts at: 2256 2373 2794 3217 3584 2256
Size: 117 421 423 367 4559
Fragments arranged by size:
4559 423 421 367 117
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3208 4663 618
Size: 325 217 627 1421 1455 1842
Fragments arranged by size:
1842 1455 1421 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3206 1391
Size: 1815 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2691 2861 3013 928
Size: 971 792 170 152 3802
Fragments arranged by size:
3802 971 792 170 152
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2225 2259 4078 4112 1903
Size: 34 288 34 1819 34 3678
Fragments arranged by size:
3678 1819 288 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3779 4989 3779
Size: 1210 4677
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5887
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4498 136
Size: 107 122 71 631 605 2826 1525
Fragments arranged by size:
2826 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5882 5882
Size: 5887
BlpI GC'TnA_GC
Cuts at: 2124 3304 2124
Size: 1180 4707
Bme1580I G_kGCm'C
Cuts at: 1790 3948 5104 1790
Size: 2158 1156 2573
Fragments arranged by size:
2573 2158 1156
BmgBI CAC'GTC
Cuts at: 1318 2761 1318
Size: 1443 4444
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2379 3618 4576
Size: 416 53 83 399 24 962 1239 958
Cuts at: 4576 442
Size: 1753
Fragments arranged by size:
1753 1239 962 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5887
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2233 2233
Size: 5887
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2416 2655 4547 1239
Size: 1177 239 1892 2579
Fragments arranged by size:
2579 1892 1177 239
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4012 4877 5175 4012
Size: 865 298 4724
Fragments arranged by size:
4724 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3102 4550 882
Size: 617 1603 1448 2219
Fragments arranged by size:
2219 1603 1448 617
BsaAI yAC'GTr
Cuts at: 493 1204 2329 2671 3678 493
Size: 711 1125 342 1007 2702
Fragments arranged by size:
2702 1125 1007 711 342
BsaBI GATnn'nnATC
Cuts at: 5881 5881
Size: 5887
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3694 4076 4711 275
Size: 53 83 186 936 2161 382 635 1451
Fragments arranged by size:
2161 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2301 3048 4320 4718 4992 5139 5550 2301
Size: 747 1272 398 274 147 411 2638
Fragments arranged by size:
2638 1272 747 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2222 2252 2291 2321 1178
Size: 30 165 30 819 30 39 30 4744
Fragments arranged by size:
4744 819 165 39 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2011 2056 2222 1186
Size: 825 45 166 4851
Fragments arranged by size:
4851 825 166 45
BseYI C'CCAG_C
Cuts at: 2097 2106 2162 5090 5586 2097
Size: 9 56 2928 496 2398
Fragments arranged by size:
2928 2398 496 56 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5314
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2067 3337 4098 5126 1540
Size: 275 186 66 1270 761 1028 2301
Fragments arranged by size:
2301 1270 1028 761 275 186 66
BsiHKAI G_wGCw'C
Cuts at: 2075 3948 4033 5104 2075
Size: 1873 85 1071 2858
Fragments arranged by size:
2858 1873 1071 85
BsmI GAATG_Cn'
Cuts at: 2432 2882 3398 3491 2432
Size: 450 516 93 4828
Fragments arranged by size:
4828 516 450 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2075 3206 3948 4033 5104 1391
Size: 399 285 1131 742 85 1071 2174
Fragments arranged by size:
2174 1131 1071 742 399 285 85
BspEI T'CCGG_A
Cuts at: 3048 5550 3048
Size: 2502 3385
BspHI T'CATG_A
Cuts at: 3776 5506 3776
Size: 1730 4157
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2756 3242 844
Size: 1912 486 3489
Fragments arranged by size:
3489 1912 486
BsrBI CCG'CTC
Cuts at: 1394 1823 3774 1394
Size: 429 1951 3507
Fragments arranged by size:
3507 1951 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2141 4382 4556 65
Size: 1183 893 2241 174 1396
Fragments arranged by size:
2241 1396 1183 893 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4531 4718 1700
Size: 110 2721 187 2869
Fragments arranged by size:
2869 2721 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5887
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5887
BssSI C'ACGA_G
Cuts at: 2261 3947 4959 2261
Size: 1686 1012 3189
Fragments arranged by size:
3189 1686 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5887
BstBI TT'CG_AA
Cuts at: 4691 4691
Size: 5887
BstEII G'GTnAC_C
Cuts at: 1184 2809 2926 1184
Size: 1625 117 4145
Fragments arranged by size:
4145 1625 117
BstXI CCAn_nnnn'nTGG
Cuts at: 2476 2476
Size: 5887
BstYI r'GATC_y
Cuts at: 1972 2256 2373 2794 3217 3584 3969 3986 5438
Size: 284 117 421 423 367 385 17 1452
Cuts at: 5438 5524 5536 5882 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 423 421 385 367 346 284
117 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2039 2039
Size: 5887
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4396
Fragments arranged by size:
4396 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5148
Fragments arranged by size:
5148 683 56
BtsI GCAGTG_nn'
Cuts at: 3403 4197 4217 3403
Size: 794 20 5073
Fragments arranged by size:
5073 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5887
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2787 2822 529
Size: 35 2223 35 3594
Fragments arranged by size:
3594 2223 35 35
DraI TTT'AAA
Cuts at: 3191 3191
Size: 5887
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4894 809
Size: 507 3578 1802
Fragments arranged by size:
3578 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2064 2763 3334
Size: 54 1425 50 275 18 234 699 571
Cuts at: 3334 4223 5852 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 699 571 275 234 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2064 3334 1537
Size: 275 252 1270 4090
Fragments arranged by size:
4090 1270 275 252
EarI CTCTTCn'nnn_
Cuts at: 1353 2508 2856 3817 1353
Size: 1155 348 961 3423
Fragments arranged by size:
3423 1155 961 348
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2069 4474 4848 4994 387
Size: 1064 138 480 2405 374 146 1280
Fragments arranged by size:
2405 1280 1064 480 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2294 2416 2655 3950 4547 5334 5602
Size: 393 662 122 239 1295 597 787 268
Cuts at: 5602 1239
Size: 1524
Fragments arranged by size:
1524 1295 787 662 597 393 268 239
122
EcoRI G'AATT_C
Cuts at: 3196 3574 3196
Size: 378 5509
FspI TGC'GCA
Cuts at: 4393 4393
Size: 5887
HaeII r_GCGC'y
Cuts at: 1678 5034 1678
Size: 3356 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4528 4560 4602 4634 1373
Size: 32 135 32 2956 32 42 32 2626
Fragments arranged by size:
2956 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3231 3477 669
Size: 988 281 1293 246 3079
Fragments arranged by size:
3079 1293 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5887
HpaI GTT'AAC
Cuts at: 3477 3477
Size: 5887
KpnI G_GTAC'C
Cuts at: 3212 3212
Size: 5887
MfeI C'AATT_G
Cuts at: 3486 3486
Size: 5887
MluI A'CGCG_T
Cuts at: 5763 5763
Size: 5887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5001 5185 1305
Size: 3696 184 2007
Fragments arranged by size:
3696 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2765 10
Size: 54 1425 343 933 3132
Fragments arranged by size:
3132 1425 933 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2078 3080 3845 4204 4363 518
Size: 648 843 69 1002 765 359 159 2042
Fragments arranged by size:
2042 1002 843 765 648 359 159 69
MspA1I CmG'CkG
Cuts at: 1606 2028 2166 3148 3303 3980 5128 5373 1606
Size: 422 138 982 155 677 1148 245 2120
Fragments arranged by size:
2120 1148 982 677 422 245 155 138
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4396
Fragments arranged by size:
4396 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5887
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5887
NotI GC'GGCC_GC
Cuts at: 3334 3334
Size: 5887
NspI r_CATG'y
Cuts at: 1132 1229 3245 4790 1132
Size: 97 2016 1545 2229
Fragments arranged by size:
2229 2016 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5887
PciI A'CATG_T
Cuts at: 4786 4786
Size: 5887
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2923 1108
Size: 1815 4072
PfoI T'CCnGG_A
Cuts at: 2754 2754
Size: 5887
PmeI GTTT'AAAC
Cuts at: 3191 3191
Size: 5887
PmlI CAC'GTG
Cuts at: 2329 2329
Size: 5887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2855 2887 3956 3988 5469 5501 2855
Size: 32 1069 32 1481 32 3241
Fragments arranged by size:
3241 1481 1069 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5887
PsiI TTA'TAA
Cuts at: 3457 3457
Size: 5887
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2297 2329 2297
Size: 32 5855
PstI C_TGCA'G
Cuts at: 830 2895 3239 830
Size: 2065 344 3478
Fragments arranged by size:
3478 2065 344
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5887
PvuII CAG'CTG
Cuts at: 2166 3148 2166
Size: 982 4905
SalI G'TCGA_C
Cuts at: 1936 3229 1936
Size: 1293 4594
SapI GCTCTTCn'nnn_
Cuts at: 2856 2856
Size: 5887
SbfI CC_TGCA'GG
Cuts at: 3239 3239
Size: 5887
ScaI AGT'ACT
Cuts at: 4135 5847 4135
Size: 1712 4175
SfcI C'TryA_G
Cuts at: 826 1045 2891 3235 4370 5051 5242 826
Size: 219 1846 344 1135 681 191 1471
Fragments arranged by size:
1846 1471 1135 681 344 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5887
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5887
SmaI CCC'GGG
Cuts at: 1872 3214 1872
Size: 1342 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2218 3991 4892 5154 820
Size: 197 926 275 1773 901 262 1553
Fragments arranged by size:
1773 1553 926 901 275 262 197
SnaBI TAC'GTA
Cuts at: 493 3678 493
Size: 3185 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5887
SphI G_CATG'C
Cuts at: 1132 3245 1132
Size: 2113 3774
SspI AAT'ATT
Cuts at: 5 52 2941 3811 5
Size: 47 2889 870 2081
Fragments arranged by size:
2889 2081 870 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5887
StyI C'CwwG_G
Cuts at: 513 1065 2004 2394 3326 513
Size: 552 939 390 932 3074
Fragments arranged by size:
3074 939 932 552 390
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3017 3914 3931 4084 4269 4714 325
Size: 1255 1437 897 17 153 185 445 1498
Fragments arranged by size:
1498 1437 1255 897 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2694 4133 5845
Size: 274 80 33 51 1443 717 1439 1712
Cuts at: 5845 96
Size: 138
Fragments arranged by size:
1712 1443 1439 717 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5847 5879 5847
Size: 32 5855
Tth111I GACn'n_nGTC
Cuts at: 1316 2912 1316
Size: 1596 4291
XbaI T'CTAG_A
Cuts at: 3223 3223
Size: 5887
XhoI C'TCGA_G
Cuts at: 1943 2218 1943
Size: 275 5612
XmaI C'CCGG_G
Cuts at: 1870 3212 1870
Size: 1342 4545
XmnI GAAnn'nnTTC
Cuts at: 4016 4016
Size: 5887
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3695 276
Size: 53 83 186 936 2161 2468
Fragments arranged by size:
2468 2161 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PsrI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI