Restriction map for
pFN21AE1870
(Circular) (Six-base) MAPSORT of: pFN21AE1870.seq Check: 4505 from: 1 to: 5878
pFN21AE1870 5878 bp
With 182 enzymes: SgfI *
March 28, 2012 12:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3688 278
Size: 53 83 186 936 2152 2468
Fragments arranged by size:
2468 2152 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2077 2130 3221 1937
Size: 140 53 1091 4594
Fragments arranged by size:
4594 1091 140 53
Acc65I G'GTAC_C
Cuts at: 3199 3199
Size: 5878
AclI AA'CG_TT
Cuts at: 1563 2631 4005 4378 1563
Size: 1068 1374 373 3063
Fragments arranged by size:
3063 1374 1068 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2495 3941 5325 5593 1632
Size: 863 1446 1384 268 1917
Fragments arranged by size:
1917 1446 1384 863 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5681
AflIII A'CryG_T
Cuts at: 1203 4777 5754 1203
Size: 3574 977 1327
Fragments arranged by size:
3574 1327 977
AgeI A'CCGG_T
Cuts at: 4709 4709
Size: 5878
AhdI GACnn_n'nnGTC
Cuts at: 4607 4607
Size: 5878
AleI CACnn'nnGTG
Cuts at: 1166 2789 1166
Size: 1623 4255
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5878
AlwNI CAG_nnn'CTG
Cuts at: 2921 3621 5193 2921
Size: 700 1572 3606
Fragments arranged by size:
3606 1572 700
ApaLI G'TGCA_C
Cuts at: 3935 5091 3935
Size: 1156 4722
ApoI r'AATT_y
Cuts at: 1493 2476 2616 2715 3187 3413 3565 5674 1493
Size: 983 140 99 472 226 152 2109 1697
Fragments arranged by size:
2109 1697 983 472 226 152 140 99
AseI AT'TA_AT
Cuts at: 160 1032 4432 160
Size: 872 3400 1606
Fragments arranged by size:
3400 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5878
AvaI C'yCGr_G
Cuts at: 1870 1943 3203 1870
Size: 73 1260 4545
Fragments arranged by size:
4545 1260 73
BamHI G'GATC_C
Cuts at: 3208 3575 3208
Size: 367 5511
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3199 4654 618
Size: 325 217 627 1412 1455 1842
Fragments arranged by size:
1842 1455 1412 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2013 2234 3197 1391
Size: 622 221 963 4072
Fragments arranged by size:
4072 963 622 221
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4907
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2170 2204 4069 4103 1903
Size: 34 233 34 1865 34 3678
Fragments arranged by size:
3678 1865 233 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3770 4980 3770
Size: 1210 4668
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5878
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4489 136
Size: 107 122 71 631 605 2817 1525
Fragments arranged by size:
2817 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5873 5873
Size: 5878
BlpI GC'TnA_GC
Cuts at: 3295 3295
Size: 5878
Bme1580I G_kGCm'C
Cuts at: 1790 3939 5095 1790
Size: 2149 1156 2573
Fragments arranged by size:
2573 2149 1156
BmgBI CAC'GTC
Cuts at: 1318 2791 1318
Size: 1473 4405
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3609 4567 442
Size: 416 53 83 399 24 2192 958 1753
Fragments arranged by size:
2192 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5878
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2045 3054 4538 1239
Size: 806 1009 1484 2579
Fragments arranged by size:
2579 1484 1009 806
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4003 4868 5166 4003
Size: 865 298 4715
Fragments arranged by size:
4715 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2388 4541 882
Size: 617 889 2153 2219
Fragments arranged by size:
2219 2153 889 617
BsaAI yAC'GTr
Cuts at: 493 1204 3669 493
Size: 711 2465 2702
Fragments arranged by size:
2702 2465 711
BsaBI GATnn'nnATC
Cuts at: 2742 5872 2742
Size: 3130 2748
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3685 4067 4702 275
Size: 53 83 186 936 2152 382 635 1451
Fragments arranged by size:
2152 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4311 4709 4983 5130 5541 4311
Size: 398 274 147 411 4648
Fragments arranged by size:
4648 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5653
Fragments arranged by size:
5653 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5878
BseYI C'CCAG_C
Cuts at: 5081 5577 5081
Size: 496 5382
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2067 2966 1163
Size: 573 331 899 4075
Fragments arranged by size:
4075 899 573 331
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3328 4089 5117 1540
Size: 275 186 1327 761 1028 2301
Fragments arranged by size:
2301 1327 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3939 4024 5095 3939
Size: 85 1071 4722
Fragments arranged by size:
4722 1071 85
BsmI GAATG_Cn'
Cuts at: 2469 3389 3482 2469
Size: 920 93 4865
Fragments arranged by size:
4865 920 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2234 3197 3939 4024 5095 1391
Size: 399 223 221 963 742 85 1071 2174
Fragments arranged by size:
2174 1071 963 742 399 223 221 85
BspEI T'CCGG_A
Cuts at: 5541 5541
Size: 5878
BspHI T'CATG_A
Cuts at: 3767 5497 3767
Size: 1730 4148
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2089 2938 3233 844
Size: 1245 849 295 3489
Fragments arranged by size:
3489 1245 849 295
BsrBI CCG'CTC
Cuts at: 1394 1823 3765 1394
Size: 429 1942 3507
Fragments arranged by size:
3507 1942 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2604 2819 4373 4547 65
Size: 1183 1356 215 1554 174 1396
Fragments arranged by size:
1554 1396 1356 1183 215 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2262 4522 4709 1700
Size: 110 452 2260 187 2869
Fragments arranged by size:
2869 2260 452 187 110
BsrGI T'GTAC_A
Cuts at: 96 2578 96
Size: 2482 3396
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5878
BssSI C'ACGA_G
Cuts at: 3938 4950 3938
Size: 1012 4866
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5878
BstBI TT'CG_AA
Cuts at: 4682 4682
Size: 5878
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5878
BstYI r'GATC_y
Cuts at: 1972 3208 3575 3960 3977 5429 5515 5527 5873
Size: 1236 367 385 17 1452 86 12 346
Cuts at: 5873 1972
Size: 1977
Fragments arranged by size:
1977 1452 1236 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2078 2131 2078
Size: 53 5825
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4387
Fragments arranged by size:
4387 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5139
Fragments arranged by size:
5139 683 56
BtsI GCAGTG_nn'
Cuts at: 3394 4188 4208 3394
Size: 794 20 5064
Fragments arranged by size:
5064 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5878
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5843
DraI TTT'AAA
Cuts at: 3182 3182
Size: 5878
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4885 809
Size: 507 3569 1802
Fragments arranged by size:
3569 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2260 3325 4214
Size: 54 1425 50 275 18 430 1065 889
Cuts at: 4214 5843 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1065 889 430 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3325 1537
Size: 275 1513 4090
Fragments arranged by size:
4090 1513 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2187 2841 3808 1353
Size: 834 654 967 3423
Fragments arranged by size:
3423 967 834 654
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4465 4839 4985 387
Size: 1064 138 2876 374 146 1280
Fragments arranged by size:
2876 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2045 2495 3054 3941 4538 5325 5593
Size: 393 413 450 559 887 597 787 268
Cuts at: 5593 1239
Size: 1524
Fragments arranged by size:
1524 887 787 597 559 450 413 393
268
EcoRI G'AATT_C
Cuts at: 3187 3565 3187
Size: 378 5500
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2789 2789
Size: 5878
FspI TGC'GCA
Cuts at: 4384 4384
Size: 5878
HaeII r_GCGC'y
Cuts at: 1678 5025 1678
Size: 3347 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2192 2224 4519 4551 4593
Size: 32 135 32 620 32 2295 32 42
Cuts at: 4593 4625 1373
Size: 32 2626
Fragments arranged by size:
2626 2295 620 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2110 3222 3468 669
Size: 988 281 172 1112 246 3079
Fragments arranged by size:
3079 1112 988 281 246 172
HindIII A'AGCT_T
Cuts at: 748 2507 2696 748
Size: 1759 189 3930
Fragments arranged by size:
3930 1759 189
HpaI GTT'AAC
Cuts at: 3468 3468
Size: 5878
KpnI G_GTAC'C
Cuts at: 3203 3203
Size: 5878
MfeI C'AATT_G
Cuts at: 2172 3477 2172
Size: 1305 4573
MluI A'CGCG_T
Cuts at: 5754 5754
Size: 5878
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2480 4992 5176 1305
Size: 1175 2512 184 2007
Fragments arranged by size:
2512 2007 1175 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4056
Fragments arranged by size:
4056 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2789 3836 4195 4354 518
Size: 648 1623 1047 359 159 2042
Fragments arranged by size:
2042 1623 1047 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2037 3294 3971 5119 5364 1606
Size: 431 1257 677 1148 245 2120
Fragments arranged by size:
2120 1257 1148 677 431 245
NaeI GCC'GGC
Cuts at: 2264 2264
Size: 5878
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4387
Fragments arranged by size:
4387 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5878
NgoMIV G'CCGG_C
Cuts at: 2262 2262
Size: 5878
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5878
NotI GC'GGCC_GC
Cuts at: 3325 3325
Size: 5878
NspI r_CATG'y
Cuts at: 1132 1229 2053 3146 3236 4781 1132
Size: 97 824 1093 90 1545 2229
Fragments arranged by size:
2229 1545 1093 824 97 90
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5878
PciI A'CATG_T
Cuts at: 4777 4777
Size: 5878
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5878
PmeI GTTT'AAAC
Cuts at: 3182 3182
Size: 5878
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3947 3979 5460 5492 3947
Size: 32 1481 32 4333
Fragments arranged by size:
4333 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5878
PsiI TTA'TAA
Cuts at: 3448 3448
Size: 5878
PstI C_TGCA'G
Cuts at: 830 2710 2938 3230 830
Size: 1880 228 292 3478
Fragments arranged by size:
3478 1880 292 228
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5878
SalI G'TCGA_C
Cuts at: 1936 3220 1936
Size: 1284 4594
SapI GCTCTTCn'nnn_
Cuts at: 2841 2841
Size: 5878
SbfI CC_TGCA'GG
Cuts at: 3230 3230
Size: 5878
ScaI AGT'ACT
Cuts at: 4126 5838 4126
Size: 1712 4166
SfcI C'TryA_G
Cuts at: 826 1045 2706 2934 3226 4361 5042 5233 826
Size: 219 1661 228 292 1135 681 191 1471
Fragments arranged by size:
1661 1471 1135 681 292 228 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5878
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5878
SmaI CCC'GGG
Cuts at: 1872 3205 1872
Size: 1333 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3982 4883 5145 820
Size: 197 926 2039 901 262 1553
Fragments arranged by size:
2039 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3669 493
Size: 3176 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5878
SphI G_CATG'C
Cuts at: 1132 2053 3236 1132
Size: 921 1183 3774
Fragments arranged by size:
3774 1183 921
SspI AAT'ATT
Cuts at: 5 52 2644 3802 5
Size: 47 2592 1158 2081
Fragments arranged by size:
2592 2081 1158 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5878
StyI C'CwwG_G
Cuts at: 513 1065 2004 3058 3317 513
Size: 552 939 1054 259 3074
Fragments arranged by size:
3074 1054 939 552 259
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3905 3922 4075 4260 4705 325
Size: 1255 2325 17 153 185 445 1498
Fragments arranged by size:
2325 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2578 4124 5836
Size: 274 80 33 51 1443 601 1546 1712
Cuts at: 5836 96
Size: 138
Fragments arranged by size:
1712 1546 1443 601 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5838 5870 5838
Size: 32 5846
Tth111I GACn'n_nGTC
Cuts at: 1316 2917 1316
Size: 1601 4277
XbaI T'CTAG_A
Cuts at: 2311 3214 2311
Size: 903 4975
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5878
XmaI C'CCGG_G
Cuts at: 1870 3203 1870
Size: 1333 4545
XmnI GAAnn'nnTTC
Cuts at: 2297 2715 4007 2297
Size: 418 1292 4168
Fragments arranged by size:
4168 1292 418
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3686 276
Size: 53 83 186 936 2152 2468
Fragments arranged by size:
2468 2152 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI
NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI XcmI