Restriction map for
pFN21AE1867
(Circular) (Six-base) MAPSORT of: pFN21AE1867.seq Check: 5440 from: 1 to: 5575
pFN21AE1867 5575 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2579 2579
Size: 5575
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2781 3385 278
Size: 53 83 186 936 1245 604 2468
Fragments arranged by size:
2468 1245 936 604 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2918 1937
Size: 981 4594
Acc65I G'GTAC_C
Cuts at: 2896 2896
Size: 5575
AclI AA'CG_TT
Cuts at: 1563 3702 4075 1563
Size: 2139 373 3063
Fragments arranged by size:
3063 2139 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2242 2255 3638 5022 5290 1632
Size: 610 13 1383 1384 268 1917
Fragments arranged by size:
1917 1384 1383 610 268 13
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5378
AflIII A'CryG_T
Cuts at: 1203 4474 5451 1203
Size: 3271 977 1327
Fragments arranged by size:
3271 1327 977
AgeI A'CCGG_T
Cuts at: 4406 4406
Size: 5575
AhdI GACnn_n'nnGTC
Cuts at: 4304 4304
Size: 5575
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5575
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2352 1184
Size: 1168 4407
AlwNI CAG_nnn'CTG
Cuts at: 2272 2290 3318 4890 2272
Size: 18 1028 1572 2957
Fragments arranged by size:
2957 1572 1028 18
ApaI G_GGCC'C
Cuts at: 2500 2500
Size: 5575
ApaLI G'TGCA_C
Cuts at: 3632 4788 3632
Size: 1156 4419
ApoI r'AATT_y
Cuts at: 1493 2460 2884 3110 3262 5371 1493
Size: 967 424 226 152 2109 1697
Fragments arranged by size:
2109 1697 967 424 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4129 160
Size: 872 3097 1606
Fragments arranged by size:
3097 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5575
AvaI C'yCGr_G
Cuts at: 1870 1943 2900 1870
Size: 73 957 4545
Fragments arranged by size:
4545 957 73
BamHI G'GATC_C
Cuts at: 2490 2905 3272 2490
Size: 415 367 4793
Fragments arranged by size:
4793 415 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2219 2896 4351 618
Size: 325 217 627 432 677 1455 1842
Fragments arranged by size:
1842 1455 677 627 432 325 217
BanII G_rGCy'C
Cuts at: 1391 2171 2500 2595 2894 1391
Size: 780 329 95 299 4072
Fragments arranged by size:
4072 780 329 299 95
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4604
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3766 3800 1903
Size: 34 50 34 1745 34 3678
Fragments arranged by size:
3678 1745 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3467 4677 3467
Size: 1210 4365
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5575
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4186 136
Size: 107 122 71 631 605 2514 1525
Fragments arranged by size:
2514 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5570 5570
Size: 5575
BlpI GC'TnA_GC
Cuts at: 2992 2992
Size: 5575
Bme1580I G_kGCm'C
Cuts at: 1790 2158 2222 2500 2765 3636 4792 1790
Size: 368 64 278 265 871 1156 2573
Fragments arranged by size:
2573 1156 871 368 278 265 64
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5575
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2655 3306 4264
Size: 416 53 83 399 24 1238 651 958
Cuts at: 4264 442
Size: 1753
Fragments arranged by size:
1753 1238 958 651 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5575
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2463 4235 1239
Size: 1224 1772 2579
Fragments arranged by size:
2579 1772 1224
Bpu10I CC'TnA_GC
Cuts at: 2170 2170
Size: 5575
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3700 4565 4863 3700
Size: 865 298 4412
Fragments arranged by size:
4412 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2039 4238 882
Size: 617 540 2199 2219
Fragments arranged by size:
2219 2199 617 540
BsaAI yAC'GTr
Cuts at: 493 1204 3366 493
Size: 711 2162 2702
Fragments arranged by size:
2702 2162 711
BsaBI GATnn'nnATC
Cuts at: 5569 5569
Size: 5575
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2778 3382 3764 4399
Size: 53 83 186 936 1245 604 382 635
Cuts at: 4399 275
Size: 1451
Fragments arranged by size:
1451 1245 936 635 604 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2319 4008 4406 4680 4827 5238 2319
Size: 1689 398 274 147 411 2656
Fragments arranged by size:
2656 1689 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2047 2077 2093 2123 1178
Size: 30 165 30 644 30 16 30 4630
Fragments arranged by size:
4630 644 165 30 30 30 30 16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2012 2114 1186
Size: 826 102 4647
Fragments arranged by size:
4647 826 102
BseYI C'CCAG_C
Cuts at: 2151 2179 2202 4778 5274 2151
Size: 28 23 2576 496 2452
Fragments arranged by size:
2576 2452 496 28 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5002
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3025 3786 4814 1540
Size: 275 186 1024 761 1028 2301
Fragments arranged by size:
2301 1028 1024 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2520 3636 3721 4792 2520
Size: 1116 85 1071 3303
Fragments arranged by size:
3303 1116 1071 85
BsmI GAATG_Cn'
Cuts at: 2404 3086 3179 2404
Size: 682 93 4800
Fragments arranged by size:
4800 682 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2158 2171 2222 2500 2520 2595 2765
Size: 399 368 13 51 278 20 75 170
Cuts at: 2765 2894 3636 3721 4792 1391
Size: 129 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 368 278 170 129
85 75 51 20 13
BspEI T'CCGG_A
Cuts at: 2319 5238 2319
Size: 2919 2656
BspHI T'CATG_A
Cuts at: 3464 5194 3464
Size: 1730 3845
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2196 2579 2930 844
Size: 1352 383 351 3489
Fragments arranged by size:
3489 1352 383 351
BsrBI CCG'CTC
Cuts at: 1394 1823 3462 1394
Size: 429 1639 3507
Fragments arranged by size:
3507 1639 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4070 4244 65
Size: 1183 2822 174 1396
Fragments arranged by size:
2822 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4219 4406 1700
Size: 110 2409 187 2869
Fragments arranged by size:
2869 2409 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5575
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5575
BssSI C'ACGA_G
Cuts at: 3635 4647 3635
Size: 1012 4563
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5575
BstBI TT'CG_AA
Cuts at: 4379 4379
Size: 5575
BstEII G'GTnAC_C
Cuts at: 1184 2183 1184
Size: 999 4576
BstXI CCAn_nnnn'nTGG
Cuts at: 2643 2643
Size: 5575
BstYI r'GATC_y
Cuts at: 1972 2370 2490 2905 3272 3657 3674 5126 5212
Size: 398 120 415 367 385 17 1452 86
Cuts at: 5212 5224 5570 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 415 398 385 367 346 120
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2248 513
Size: 552 1183 3840
Fragments arranged by size:
3840 1183 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4836
Fragments arranged by size:
4836 683 56
BtsI GCAGTG_nn'
Cuts at: 3091 3885 3905 3091
Size: 794 20 4761
Fragments arranged by size:
4761 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5575
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5540
DraI TTT'AAA
Cuts at: 2879 2879
Size: 5575
DraIII CAC_nnn'GTG
Cuts at: 2162 2162
Size: 5575
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4582 809
Size: 507 3266 1802
Fragments arranged by size:
3266 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2674 3022 3911
Size: 54 1425 50 275 18 844 348 889
Cuts at: 3911 5540 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 844 348 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3022 1537
Size: 275 1210 4090
Fragments arranged by size:
4090 1210 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2030 3505 1353
Size: 677 1475 3423
Fragments arranged by size:
3423 1475 677
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4162 4536 4682 387
Size: 1064 138 2573 374 146 1280
Fragments arranged by size:
2573 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2242 2255 2463 3638 4235 5022 5290
Size: 393 610 13 208 1175 597 787 268
Cuts at: 5290 1239
Size: 1524
Fragments arranged by size:
1524 1175 787 610 597 393 268 208
13
EcoNI CCTnn'n_nnAGG
Cuts at: 2027 2027
Size: 5575
EcoRI G'AATT_C
Cuts at: 2884 3262 2884
Size: 378 5197
FspI TGC'GCA
Cuts at: 4081 4081
Size: 5575
HaeII r_GCGC'y
Cuts at: 1678 4722 1678
Size: 3044 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2093 2125 2475 2507 2763
Size: 32 135 32 521 32 350 32 256
Cuts at: 2763 2795 2800 2832 4216 4248 4290 4322 1373
Size: 32 5 32 1384 32 42 32 2626
Fragments arranged by size:
2626 1384 521 350 256 135 42 32
32 32 32 32 32 32 32 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2919 3165 669
Size: 988 281 981 246 3079
Fragments arranged by size:
3079 988 981 281 246
HindIII A'AGCT_T
Cuts at: 748 2331 748
Size: 1583 3992
HpaI GTT'AAC
Cuts at: 3165 3165
Size: 5575
KpnI G_GTAC'C
Cuts at: 2900 2900
Size: 5575
MfeI C'AATT_G
Cuts at: 3174 3174
Size: 5575
MluI A'CGCG_T
Cuts at: 5451 5451
Size: 5575
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4689 4873 1305
Size: 3384 184 2007
Fragments arranged by size:
3384 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3753
Fragments arranged by size:
3753 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3533 3892 4051 518
Size: 648 2367 359 159 2042
Fragments arranged by size:
2367 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2107 2151 2758 2991 3668 4816 5061 1606
Size: 501 44 607 233 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 607 501 245 233 44
NcoI C'CATG_G
Cuts at: 513 1065 2248 513
Size: 552 1183 3840
Fragments arranged by size:
3840 1183 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5575
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5575
NotI GC'GGCC_GC
Cuts at: 3022 3022
Size: 5575
NspI r_CATG'y
Cuts at: 1132 1229 2122 2933 4478 1132
Size: 97 893 811 1545 2229
Fragments arranged by size:
2229 1545 893 811 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5575
PciI A'CATG_T
Cuts at: 4474 4474
Size: 5575
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5575
PfoI T'CCnGG_A
Cuts at: 2479 2479
Size: 5575
PmeI GTTT'AAAC
Cuts at: 2879 2879
Size: 5575
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3644 3676 5157 5189 3644
Size: 32 1481 32 4030
Fragments arranged by size:
4030 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5575
PsiI TTA'TAA
Cuts at: 3145 3145
Size: 5575
PspOMI G'GGCC_C
Cuts at: 2496 2496
Size: 5575
PstI C_TGCA'G
Cuts at: 830 2193 2685 2927 830
Size: 1363 492 242 3478
Fragments arranged by size:
3478 1363 492 242
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5575
PvuII CAG'CTG
Cuts at: 2107 2151 2107
Size: 44 5531
SalI G'TCGA_C
Cuts at: 1936 2917 1936
Size: 981 4594
SbfI CC_TGCA'GG
Cuts at: 2927 2927
Size: 5575
ScaI AGT'ACT
Cuts at: 3823 5535 3823
Size: 1712 3863
SfcI C'TryA_G
Cuts at: 826 1045 2189 2389 2681 2923 4058 4739 4930
Size: 219 1144 200 292 242 1135 681 191
Cuts at: 4930 826
Size: 1471
Fragments arranged by size:
1471 1144 1135 681 292 242 219 200
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5575
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5575
SmaI CCC'GGG
Cuts at: 1872 2902 1872
Size: 1030 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3679 4580 4842 820
Size: 197 926 1736 901 262 1553
Fragments arranged by size:
1736 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3366 493
Size: 2873 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5575
SphI G_CATG'C
Cuts at: 1132 2122 2933 1132
Size: 990 811 3774
Fragments arranged by size:
3774 990 811
SspI AAT'ATT
Cuts at: 5 52 3499 5
Size: 47 3447 2081
Fragments arranged by size:
3447 2081 47
StuI AGG'CCT
Cuts at: 1517 2642 1517
Size: 1125 4450
StyI C'CwwG_G
Cuts at: 513 1065 2248 2455 3014 513
Size: 552 1183 207 559 3074
Fragments arranged by size:
3074 1183 559 552 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2171 2716 3602 3619 3772 3957 4402
Size: 1255 591 545 886 17 153 185 445
Cuts at: 4402 325
Size: 1498
Fragments arranged by size:
1498 1255 886 591 545 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3821 5533 96
Size: 274 80 33 51 1443 1844 1712 138
Fragments arranged by size:
1844 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2728 2760 5535 5567 2728
Size: 32 2775 32 2736
Fragments arranged by size:
2775 2736 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2210 1316
Size: 894 4681
XbaI T'CTAG_A
Cuts at: 2911 2911
Size: 5575
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2381 2721 2381
Size: 340 5235
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5575
XmaI C'CCGG_G
Cuts at: 1870 2900 1870
Size: 1030 4545
XmnI GAAnn'nnTTC
Cuts at: 3704 3704
Size: 5575
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2779 3383 276
Size: 53 83 186 936 1245 604 2468
Fragments arranged by size:
2468 1245 936 604 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI