Restriction map for
pFN21AE1865
(Circular) (Six-base) MAPSORT of: pFN21AE1865.seq Check: 1167 from: 1 to: 5863
pFN21AE1865 5863 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2419 2419
Size: 5863
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3673 278
Size: 53 83 186 936 2137 2468
Fragments arranged by size:
2468 2137 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3134 3206 1937
Size: 1197 72 4594
Fragments arranged by size:
4594 1197 72
Acc65I G'GTAC_C
Cuts at: 3184 3184
Size: 5863
AclI AA'CG_TT
Cuts at: 1563 3990 4363 1563
Size: 2427 373 3063
Fragments arranged by size:
3063 2427 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2894 3026 3926 5310 5578 1632
Size: 1262 132 900 1384 268 1917
Fragments arranged by size:
1917 1384 1262 900 268 132
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5666
AflIII A'CryG_T
Cuts at: 1203 2079 2235 4762 5739 1203
Size: 876 156 2527 977 1327
Fragments arranged by size:
2527 1327 977 876 156
AgeI A'CCGG_T
Cuts at: 4694 4694
Size: 5863
AhdI GACnn_n'nnGTC
Cuts at: 4592 4592
Size: 5863
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5863
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2153 1184
Size: 969 4894
AlwNI CAG_nnn'CTG
Cuts at: 3606 5178 3606
Size: 1572 4291
ApaI G_GGCC'C
Cuts at: 2398 2398
Size: 5863
ApaLI G'TGCA_C
Cuts at: 2606 3920 5076 2606
Size: 1314 1156 3393
Fragments arranged by size:
3393 1314 1156
ApoI r'AATT_y
Cuts at: 1493 2879 2894 3067 3172 3398 3550 5659 1493
Size: 1386 15 173 105 226 152 2109 1697
Fragments arranged by size:
2109 1697 1386 226 173 152 105 15
AseI AT'TA_AT
Cuts at: 160 1032 4417 160
Size: 872 3385 1606
Fragments arranged by size:
3385 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5863
AvaI C'yCGr_G
Cuts at: 1870 1943 2123 2448 2625 2643 2844 2934 3188
Size: 73 180 325 177 18 201 90 254
Cuts at: 3188 1870
Size: 4545
Fragments arranged by size:
4545 325 254 201 180 177 90 73
18
BamHI G'GATC_C
Cuts at: 3193 3560 3193
Size: 367 5496
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3184 4639 618
Size: 325 217 627 1397 1455 1842
Fragments arranged by size:
1842 1455 1397 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2226 2398 2455 2851 3182 1391
Size: 835 172 57 396 331 4072
Fragments arranged by size:
4072 835 396 331 172 57
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2142 2299 928
Size: 971 243 157 4492
Fragments arranged by size:
4492 971 243 157
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4054 4088 1903
Size: 34 50 34 2033 34 3678
Fragments arranged by size:
3678 2033 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3755 4965 3755
Size: 1210 4653
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5863
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2063 2230 2855
Size: 107 122 71 631 605 391 167 625
Cuts at: 2855 4474 136
Size: 1619 1525
Fragments arranged by size:
1619 1525 631 625 605 391 167 122
107 71
BglII A'GATC_T
Cuts at: 2662 2769 3092 5858 2662
Size: 107 323 2766 2667
Fragments arranged by size:
2766 2667 323 107
BlpI GC'TnA_GC
Cuts at: 3280 3280
Size: 5863
Bme1580I G_kGCm'C
Cuts at: 1790 2398 2610 3924 5080 1790
Size: 608 212 1314 1156 2573
Fragments arranged by size:
2573 1314 1156 608 212
BmgBI CAC'GTC
Cuts at: 1318 3088 1318
Size: 1770 4093
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3594 4552 442
Size: 416 53 83 399 24 2177 958 1753
Fragments arranged by size:
2177 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5863
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2786 4523 1239
Size: 1547 1737 2579
Fragments arranged by size:
2579 1737 1547
Bpu10I CC'TnA_GC
Cuts at: 3150 3150
Size: 5863
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2516 2696 3988 4853 5151 2516
Size: 180 1292 865 298 3228
Fragments arranged by size:
3228 1292 865 298 180
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4526 882
Size: 617 3027 2219
Fragments arranged by size:
3027 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2137 2236 2524 3654 493
Size: 711 933 99 288 1130 2702
Fragments arranged by size:
2702 1130 933 711 288 99
BsaBI GATnn'nnATC
Cuts at: 2057 5857 2057
Size: 3800 2063
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3670 4052 4687 275
Size: 53 83 186 936 2137 382 635 1451
Fragments arranged by size:
2137 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4296 4694 4968 5115 5526 4296
Size: 398 274 147 411 4633
Fragments arranged by size:
4633 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2084 2114 1178
Size: 30 165 30 681 30 4927
Fragments arranged by size:
4927 681 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5863
BseYI C'CCAG_C
Cuts at: 3073 5066 5562 3073
Size: 1993 496 3374
Fragments arranged by size:
3374 1993 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2258 2391 2491 1163
Size: 573 522 133 100 4535
Fragments arranged by size:
4535 573 522 133 100
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3313 4074 5102 1540
Size: 275 186 1312 761 1028 2301
Fragments arranged by size:
2301 1312 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2261 2532 2610 3924 4009 5080 2261
Size: 271 78 1314 85 1071 3044
Fragments arranged by size:
3044 1314 1071 271 85 78
BsmI GAATG_Cn'
Cuts at: 3374 3467 3374
Size: 93 5770
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2226 2261 2398 2455 2532 2610 2851
Size: 399 436 35 137 57 77 78 241
Cuts at: 2851 3182 3924 4009 5080 1391
Size: 331 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 436 399 331 241 137
85 78 77 57 35
BspEI T'CCGG_A
Cuts at: 5526 5526
Size: 5863
BspHI T'CATG_A
Cuts at: 2667 3752 5482 2667
Size: 1085 1730 3048
Fragments arranged by size:
3048 1730 1085
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2419 2562 2604 2734 2826 3218 844
Size: 1575 143 42 130 92 392 3489
Fragments arranged by size:
3489 1575 392 143 130 92 42
BsrBI CCG'CTC
Cuts at: 1394 1823 2511 3750 1394
Size: 429 688 1239 3507
Fragments arranged by size:
3507 1239 688 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4358 4532 65
Size: 1183 3110 174 1396
Fragments arranged by size:
3110 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4507 4694 1700
Size: 110 2697 187 2869
Fragments arranged by size:
2869 2697 187 110
BsrGI T'GTAC_A
Cuts at: 96 2082 96
Size: 1986 3877
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5863
BssSI C'ACGA_G
Cuts at: 3923 4935 3923
Size: 1012 4851
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2605 794
Size: 1811 4052
BstBI TT'CG_AA
Cuts at: 4667 4667
Size: 5863
BstEII G'GTnAC_C
Cuts at: 1184 2951 1184
Size: 1767 4096
BstYI r'GATC_y
Cuts at: 1972 2290 2662 2769 3092 3124 3193 3560 3945
Size: 318 372 107 323 32 69 367 385
Cuts at: 3945 3962 5414 5500 5512 5858 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 372 367 346 323 318
107 86 69 32 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5311
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3072 487
Size: 683 56 1846 3278
Fragments arranged by size:
3278 1846 683 56
BtsI GCAGTG_nn'
Cuts at: 2455 3379 4173 4193 2455
Size: 924 794 20 4125
Fragments arranged by size:
4125 924 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5863
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5828
DraI TTT'AAA
Cuts at: 3167 3167
Size: 5863
DraIII CAC_nnn'GTG
Cuts at: 2527 2527
Size: 5863
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2324 2785 4870 809
Size: 507 1008 461 2085 1802
Fragments arranged by size:
2085 1802 1008 507 461
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 3310 4199
Size: 54 1425 50 275 18 225 1255 889
Cuts at: 4199 5828 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1255 889 275 225 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3310 1537
Size: 275 1498 4090
Fragments arranged by size:
4090 1498 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2173 3793 1353
Size: 820 1620 3423
Fragments arranged by size:
3423 1620 820
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4450 4824 4970 387
Size: 1064 138 2861 374 146 1280
Fragments arranged by size:
2861 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2786 2894 3026 3926 4523 5310 5578
Size: 393 1154 108 132 900 597 787 268
Cuts at: 5578 1239
Size: 1524
Fragments arranged by size:
1524 1154 900 787 597 393 268 132
108
EcoO109I rG'GnC_Cy
Cuts at: 2395 2620 2800 2836 2395
Size: 225 180 36 5422
Fragments arranged by size:
5422 225 180 36
EcoRI G'AATT_C
Cuts at: 2879 2894 3172 3550 2879
Size: 15 278 378 5192
Fragments arranged by size:
5192 378 278 15
FspI TGC'GCA
Cuts at: 4369 4369
Size: 5863
HaeII r_GCGC'y
Cuts at: 1678 2161 3010 5010 1678
Size: 483 849 2000 2531
Fragments arranged by size:
2531 2000 849 483
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2662 2694 3116 3148 4504
Size: 32 135 32 1090 32 422 32 1356
Cuts at: 4504 4536 4578 4610 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1356 1090 422 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2477 3207 3453 669
Size: 988 281 539 730 246 3079
Fragments arranged by size:
3079 988 730 539 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5863
HpaI GTT'AAC
Cuts at: 3453 3453
Size: 5863
KpnI G_GTAC'C
Cuts at: 3188 3188
Size: 5863
MfeI C'AATT_G
Cuts at: 3462 3462
Size: 5863
MluI A'CGCG_T
Cuts at: 5739 5739
Size: 5863
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4977 5161 1305
Size: 3672 184 2007
Fragments arranged by size:
3672 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2057 10
Size: 54 1425 343 225 3816
Fragments arranged by size:
3816 1425 343 225 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2135 2202 2282 2687 2970 3821 4180
Size: 648 969 67 80 405 283 851 359
Cuts at: 4180 4339 518
Size: 159 2042
Fragments arranged by size:
2042 969 851 648 405 359 283 159
80 67
MspA1I CmG'CkG
Cuts at: 1606 2572 2764 3279 3956 5104 5349 1606
Size: 966 192 515 677 1148 245 2120
Fragments arranged by size:
2120 1148 966 677 515 245 192
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5311
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5863
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5863
NotI GC'GGCC_GC
Cuts at: 3310 3310
Size: 5863
NspI r_CATG'y
Cuts at: 1132 1229 2083 2134 3221 4766 1132
Size: 97 854 51 1087 1545 2229
Fragments arranged by size:
2229 1545 1087 854 97 51
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5863
PciI A'CATG_T
Cuts at: 2079 4762 2079
Size: 2683 3180
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5863
PfoI T'CCnGG_A
Cuts at: 2616 2616
Size: 5863
PmeI GTTT'AAAC
Cuts at: 3167 3167
Size: 5863
PmlI CAC'GTG
Cuts at: 2137 2236 2524 2137
Size: 99 288 5476
Fragments arranged by size:
5476 288 99
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3932 3964 5445 5477 3932
Size: 32 1481 32 4318
Fragments arranged by size:
4318 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2620 2836 2620
Size: 216 5647
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5863
PsiI TTA'TAA
Cuts at: 3433 3433
Size: 5863
PspOMI G'GGCC_C
Cuts at: 2394 2394
Size: 5863
PspXI vC'TCGA_Gb
Cuts at: 2844 2844
Size: 5863
PstI C_TGCA'G
Cuts at: 830 2462 2559 2808 3215 830
Size: 1632 97 249 407 3478
Fragments arranged by size:
3478 1632 407 249 97
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5863
PvuII CAG'CTG
Cuts at: 2764 2764
Size: 5863
SalI G'TCGA_C
Cuts at: 1936 3205 1936
Size: 1269 4594
SanDI GG'GwC_CC
Cuts at: 2620 2620
Size: 5863
SapI GCTCTTCn'nnn_
Cuts at: 2173 2173
Size: 5863
SbfI CC_TGCA'GG
Cuts at: 2808 3215 2808
Size: 407 5456
ScaI AGT'ACT
Cuts at: 4111 5823 4111
Size: 1712 4151
SexAI A'CCwGG_T
Cuts at: 2347 2610 2347
Size: 263 5600
SfcI C'TryA_G
Cuts at: 826 1045 2458 2515 2555 2804 3211 4346 5027
Size: 219 1413 57 40 249 407 1135 681
Cuts at: 5027 5218 826
Size: 191 1471
Fragments arranged by size:
1471 1413 1135 681 407 249 219 191
57 40
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5863
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5863
SmaI CCC'GGG
Cuts at: 1872 2450 2627 3190 1872
Size: 578 177 563 4545
Fragments arranged by size:
4545 578 563 177
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2531 2711 2844 3967 4868 5130
Size: 197 926 588 180 133 1123 901 262
Cuts at: 5130 820
Size: 1553
Fragments arranged by size:
1553 1123 926 901 588 262 197 180
133
SnaBI TAC'GTA
Cuts at: 493 3654 493
Size: 3161 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5863
SphI G_CATG'C
Cuts at: 1132 2134 3221 1132
Size: 1002 1087 3774
Fragments arranged by size:
3774 1087 1002
SspI AAT'ATT
Cuts at: 5 52 3787 5
Size: 47 3735 2081
Fragments arranged by size:
3735 2081 47
StuI AGG'CCT
Cuts at: 1517 2504 1517
Size: 987 4876
StyI C'CwwG_G
Cuts at: 513 1065 2796 3302 513
Size: 552 1731 506 3074
Fragments arranged by size:
3074 1731 552 506
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2214 2908 2939 3890 3907 4060 4245
Size: 1255 634 694 31 951 17 153 185
Cuts at: 4245 4690 325
Size: 445 1498
Fragments arranged by size:
1498 1255 951 694 634 445 185 153
31 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2082 4109 5821
Size: 274 80 33 51 1443 105 2027 1712
Cuts at: 5821 96
Size: 138
Fragments arranged by size:
2027 1712 1443 274 138 105 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5823 5855 5823
Size: 32 5831
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5863
XbaI T'CTAG_A
Cuts at: 3199 3199
Size: 5863
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3070 3070
Size: 5863
XhoI C'TCGA_G
Cuts at: 1943 2844 1943
Size: 901 4962
XmaI C'CCGG_G
Cuts at: 1870 2448 2625 3188 1870
Size: 578 177 563 4545
Fragments arranged by size:
4545 578 563 177
XmnI GAAnn'nnTTC
Cuts at: 3992 3992
Size: 5863
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3671 276
Size: 53 83 186 936 2137 2468
Fragments arranged by size:
2468 2137 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SalI SanDI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PsrI RsrII SacI SacII SfiI
SfoI SrfI SwaI