Restriction map for
pFN21AE1862
(Circular) (Six-base) MAPSORT of: pFN21AE1862.seq Check: 7758 from: 1 to: 5848
pFN21AE1862 5848 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2244 3658 278
Size: 53 83 186 936 708 1414 2468
Fragments arranged by size:
2468 1414 936 708 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3191 1937
Size: 1254 4594
Acc65I G'GTAC_C
Cuts at: 3169 3169
Size: 5848
AclI AA'CG_TT
Cuts at: 1563 3975 4348 1563
Size: 2412 373 3063
Fragments arranged by size:
3063 2412 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2799 3911 5295 5563 1632
Size: 1167 1112 1384 268 1917
Fragments arranged by size:
1917 1384 1167 1112 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5651
AflIII A'CryG_T
Cuts at: 1203 4747 5724 1203
Size: 3544 977 1327
Fragments arranged by size:
3544 1327 977
AgeI A'CCGG_T
Cuts at: 4679 4679
Size: 5848
AhdI GACnn_n'nnGTC
Cuts at: 4577 4577
Size: 5848
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5848
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2031 2240 1184
Size: 847 209 4792
Fragments arranged by size:
4792 847 209
AlwNI CAG_nnn'CTG
Cuts at: 2266 3591 5163 2266
Size: 1325 1572 2951
Fragments arranged by size:
2951 1572 1325
ApaLI G'TGCA_C
Cuts at: 3905 5061 3905
Size: 1156 4692
ApoI r'AATT_y
Cuts at: 1493 2089 2827 3157 3383 3535 5644 1493
Size: 596 738 330 226 152 2109 1697
Fragments arranged by size:
2109 1697 738 596 330 226 152
AscI GG'CGCG_CC
Cuts at: 2443 2443
Size: 5848
AseI AT'TA_AT
Cuts at: 160 1032 4402 160
Size: 872 3370 1606
Fragments arranged by size:
3370 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5848
AvaI C'yCGr_G
Cuts at: 1870 1943 3173 1870
Size: 73 1230 4545
Fragments arranged by size:
4545 1230 73
BamHI G'GATC_C
Cuts at: 3178 3545 3178
Size: 367 5481
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2819 3169 4624 618
Size: 325 217 627 1032 350 1455 1842
Fragments arranged by size:
1842 1455 1032 627 350 325 217
BanII G_rGCy'C
Cuts at: 1391 2326 2844 3136 3167 1391
Size: 935 518 292 31 4072
Fragments arranged by size:
4072 935 518 292 31
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4877
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4039 4073 1903
Size: 34 50 34 2018 34 3678
Fragments arranged by size:
3678 2018 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3740 4950 3740
Size: 1210 4638
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5848
BfrBI ATG'CAT
Cuts at: 2008 2008
Size: 5848
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4459 136
Size: 107 122 71 631 605 2787 1525
Fragments arranged by size:
2787 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5843 5843
Size: 5848
BlpI GC'TnA_GC
Cuts at: 3265 3265
Size: 5848
Bme1580I G_kGCm'C
Cuts at: 1790 3909 5065 1790
Size: 2119 1156 2573
Fragments arranged by size:
2573 2119 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5848
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3579 4537 442
Size: 416 53 83 399 24 2162 958 1753
Fragments arranged by size:
2162 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5848
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2213 4508 1239
Size: 974 2295 2579
Fragments arranged by size:
2579 2295 974
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2636 3973 4838 5136 2636
Size: 1337 865 298 3348
Fragments arranged by size:
3348 1337 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2596 3047 4511 882
Size: 617 1097 451 1464 2219
Fragments arranged by size:
2219 1464 1097 617 451
BsaAI yAC'GTr
Cuts at: 493 1204 3639 493
Size: 711 2435 2702
Fragments arranged by size:
2702 2435 711
BsaBI GATnn'nnATC
Cuts at: 2073 5842 2073
Size: 3769 2079
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2241 3655 4037 4672
Size: 53 83 186 936 708 1414 382 635
Cuts at: 4672 275
Size: 1451
Fragments arranged by size:
1451 1414 936 708 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3056 4281 4679 4953 5100 5511 3056
Size: 1225 398 274 147 411 3393
Fragments arranged by size:
3393 1225 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5623
Fragments arranged by size:
5623 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2288 2447 2480 2774 3075 3144 1186
Size: 1102 159 33 294 301 69 3890
Fragments arranged by size:
3890 1102 301 294 159 69 33
BseYI C'CCAG_C
Cuts at: 2278 2508 5051 5547 2278
Size: 230 2543 496 2579
Fragments arranged by size:
2579 2543 496 230
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2477 2522 1163
Size: 573 741 45 4489
Fragments arranged by size:
4489 741 573 45
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3298 4059 5087 1540
Size: 275 186 1297 761 1028 2301
Fragments arranged by size:
2301 1297 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2035 2163 2353 3136 3909 3994 5065 2035
Size: 128 190 783 773 85 1071 2818
Fragments arranged by size:
2818 1071 783 773 190 128 85
BsmI GAATG_Cn'
Cuts at: 3359 3452 3359
Size: 93 5755
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2035 2163 2326 2353 2844 3136 3167
Size: 399 245 128 163 27 491 292 31
Cuts at: 3167 3909 3994 5065 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 491 399 292 245 163
128 85 31 27
BspEI T'CCGG_A
Cuts at: 5511 5511
Size: 5848
BspHI T'CATG_A
Cuts at: 3737 5467 3737
Size: 1730 4118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2499 2525 3203 844
Size: 1655 26 678 3489
Fragments arranged by size:
3489 1655 678 26
BsrBI CCG'CTC
Cuts at: 1394 1823 3735 1394
Size: 429 1912 3507
Fragments arranged by size:
3507 1912 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3043 4343 4517 65
Size: 1183 1795 1300 174 1396
Fragments arranged by size:
1795 1396 1300 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2283 2530 4492 4679 1700
Size: 110 473 247 1962 187 2869
Fragments arranged by size:
2869 1962 473 247 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5848
BssHII G'CGCG_C
Cuts at: 1925 2313 2443 1925
Size: 388 130 5330
Fragments arranged by size:
5330 388 130
BssSI C'ACGA_G
Cuts at: 3908 4920 3908
Size: 1012 4836
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2266 2500 794
Size: 1472 234 4142
Fragments arranged by size:
4142 1472 234
BstBI TT'CG_AA
Cuts at: 4652 4652
Size: 5848
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5848
BstXI CCAn_nnnn'nTGG
Cuts at: 2353 3125 2353
Size: 772 5076
BstYI r'GATC_y
Cuts at: 1972 3114 3178 3545 3930 3947 5399 5485 5497
Size: 1142 64 367 385 17 1452 86 12
Cuts at: 5497 5843 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1142 385 367 346 86 64
17 12
Bsu36I CC'TnA_GG
Cuts at: 2913 2913
Size: 5848
BtgI C'CryG_G
Cuts at: 513 1065 2232 2454 2784 513
Size: 552 1167 222 330 3577
Fragments arranged by size:
3577 1167 552 330 222
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5109
Fragments arranged by size:
5109 683 56
BtsI GCAGTG_nn'
Cuts at: 2052 3364 4158 4178 2052
Size: 1312 794 20 3722
Fragments arranged by size:
3722 1312 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5848
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5813
DraI TTT'AAA
Cuts at: 3152 3152
Size: 5848
DraIII CAC_nnn'GTG
Cuts at: 2142 2500 2142
Size: 358 5490
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4855 809
Size: 507 3539 1802
Fragments arranged by size:
3539 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2052 2451 2892
Size: 54 1425 50 275 18 222 399 441
Cuts at: 2892 2935 3295 4184 5813 8
Size: 43 360 889 1629 43
Fragments arranged by size:
1629 1425 889 441 399 360 275 222
54 50 43 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3295 1537
Size: 275 1483 4090
Fragments arranged by size:
4090 1483 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2186 3036 3778 1353
Size: 833 850 742 3423
Fragments arranged by size:
3423 850 833 742
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4435 4809 4955 387
Size: 1064 138 2846 374 146 1280
Fragments arranged by size:
2846 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3134 3134
Size: 5848
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2213 2799 3911 4508 5295 5563 1239
Size: 393 581 586 1112 597 787 268 1524
Fragments arranged by size:
1524 1112 787 597 586 581 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2916 2916
Size: 5848
EcoO109I rG'GnC_Cy
Cuts at: 2131 2131
Size: 5848
EcoRI G'AATT_C
Cuts at: 2827 3157 3535 2827
Size: 330 378 5140
Fragments arranged by size:
5140 378 330
FspI TGC'GCA
Cuts at: 4354 4354
Size: 5848
HaeII r_GCGC'y
Cuts at: 1678 2428 4995 1678
Size: 750 2567 2531
Fragments arranged by size:
2567 2531 750
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2335 2367 4489 4521 4563
Size: 32 135 32 763 32 2122 32 42
Cuts at: 4563 4595 1373
Size: 32 2626
Fragments arranged by size:
2626 2122 763 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3192 3438 669
Size: 988 281 1254 246 3079
Fragments arranged by size:
3079 1254 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5848
HpaI GTT'AAC
Cuts at: 3438 3438
Size: 5848
KpnI G_GTAC'C
Cuts at: 3173 3173
Size: 5848
MfeI C'AATT_G
Cuts at: 3447 3447
Size: 5848
MluI A'CGCG_T
Cuts at: 5724 5724
Size: 5848
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4962 5146 1305
Size: 3657 184 2007
Fragments arranged by size:
3657 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2894 2937 10
Size: 54 1425 343 1062 43 2921
Fragments arranged by size:
2921 1425 1062 343 54 43
MslI CAynn'nnrTG
Cuts at: 518 1166 2084 2851 3806 4165 4324 518
Size: 648 918 767 955 359 159 2042
Fragments arranged by size:
2042 955 918 767 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2456 2716 3264 3941 5089 5334 1606
Size: 850 260 548 677 1148 245 2120
Fragments arranged by size:
2120 1148 850 677 548 260 245
NaeI GCC'GGC
Cuts at: 2285 2532 2285
Size: 247 5601
NcoI C'CATG_G
Cuts at: 513 1065 2784 513
Size: 552 1719 3577
Fragments arranged by size:
3577 1719 552
NdeI CA'TA_TG
Cuts at: 387 2704 387
Size: 2317 3531
NgoMIV G'CCGG_C
Cuts at: 2283 2530 2283
Size: 247 5601
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5848
NotI GC'GGCC_GC
Cuts at: 3295 3295
Size: 5848
NsiI A_TGCA'T
Cuts at: 2010 2010
Size: 5848
NspI r_CATG'y
Cuts at: 1132 1229 2221 3206 4751 1132
Size: 97 992 985 1545 2229
Fragments arranged by size:
2229 1545 992 985 97
PasI CC'CwG_GG
Cuts at: 1348 2794 1348
Size: 1446 4402
PciI A'CATG_T
Cuts at: 4747 4747
Size: 5848
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5848
PmeI GTTT'AAAC
Cuts at: 3152 3152
Size: 5848
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3917 3949 5430 5462 3917
Size: 32 1481 32 4303
Fragments arranged by size:
4303 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2131 2131
Size: 5848
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5848
PsiI TTA'TAA
Cuts at: 3418 3418
Size: 5848
PstI C_TGCA'G
Cuts at: 830 2047 2262 2370 2931 3200 830
Size: 1217 215 108 561 269 3478
Fragments arranged by size:
3478 1217 561 269 215 108
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5848
PvuII CAG'CTG
Cuts at: 2716 2716
Size: 5848
SacI G_AGCT'C
Cuts at: 3136 3136
Size: 5848
SacII CC_GC'GG
Cuts at: 2457 2457
Size: 5848
SalI G'TCGA_C
Cuts at: 1936 3190 1936
Size: 1254 4594
SapI GCTCTTCn'nnn_
Cuts at: 2186 2186
Size: 5848
SbfI CC_TGCA'GG
Cuts at: 3200 3200
Size: 5848
ScaI AGT'ACT
Cuts at: 4096 5808 4096
Size: 1712 4136
SfcI C'TryA_G
Cuts at: 826 1045 2043 2258 2366 2927 3196 4331 5012
Size: 219 998 215 108 561 269 1135 681
Cuts at: 5012 5203 826
Size: 191 1471
Fragments arranged by size:
1471 1135 998 681 561 269 219 215
191 108
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5848
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5848
SmaI CCC'GGG
Cuts at: 1872 3175 1872
Size: 1303 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2615 3952 4853 5115 820
Size: 197 926 672 1337 901 262 1553
Fragments arranged by size:
1553 1337 926 901 672 262 197
SnaBI TAC'GTA
Cuts at: 493 3639 493
Size: 3146 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5848
SphI G_CATG'C
Cuts at: 1132 2221 3206 1132
Size: 1089 985 3774
Fragments arranged by size:
3774 1089 985
SspI AAT'ATT
Cuts at: 5 52 3091 3772 5
Size: 47 3039 681 2081
Fragments arranged by size:
3039 2081 681 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5848
StyI C'CwwG_G
Cuts at: 513 1065 2784 3287 513
Size: 552 1719 503 3074
Fragments arranged by size:
3074 1719 552 503
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2741 2951 3087 3875 3892 4045 4230
Size: 1255 1161 210 136 788 17 153 185
Cuts at: 4230 4675 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1161 788 445 210 185 153
136 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4094 5806 96
Size: 274 80 33 51 1443 2117 1712 138
Fragments arranged by size:
2117 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5808 5840 5808
Size: 32 5816
Tth111I GACn'n_nGTC
Cuts at: 1316 2240 1316
Size: 924 4924
XbaI T'CTAG_A
Cuts at: 3184 3184
Size: 5848
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2743 2791 2887 2945 2743
Size: 48 96 58 5646
Fragments arranged by size:
5646 96 58 48
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5848
XmaI C'CCGG_G
Cuts at: 1870 3173 1870
Size: 1303 4545
XmnI GAAnn'nnTTC
Cuts at: 3977 3977
Size: 5848
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2242 3656 276
Size: 53 83 186 936 708 1414 2468
Fragments arranged by size:
2468 1414 936 708 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SacI SacII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I EcoRV FalI FseI
FspAI KasI NarI NruI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SanDI SexAI SfiI SfoI SrfI
SwaI