Restriction map for
pFN21AE1861
(Circular) (Six-base) MAPSORT of: pFN21AE1861.seq Check: 4272 from: 1 to: 5845
pFN21AE1861 5845 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2495 2495
Size: 5845
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2990 3655 278
Size: 53 83 186 936 1454 665 2468
Fragments arranged by size:
2468 1454 936 665 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2904 3188 1937
Size: 967 284 4594
Fragments arranged by size:
4594 967 284
Acc65I G'GTAC_C
Cuts at: 3166 3166
Size: 5845
AclI AA'CG_TT
Cuts at: 1563 3972 4345 1563
Size: 2409 373 3063
Fragments arranged by size:
3063 2409 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2771 2822 3908 5292 5560 1632
Size: 1139 51 1086 1384 268 1917
Fragments arranged by size:
1917 1384 1139 1086 268 51
AfeI AGC'GCT
Cuts at: 3065 3065
Size: 5845
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5648
AflIII A'CryG_T
Cuts at: 1203 4744 5721 1203
Size: 3541 977 1327
Fragments arranged by size:
3541 1327 977
AgeI A'CCGG_T
Cuts at: 4676 4676
Size: 5845
AhdI GACnn_n'nnGTC
Cuts at: 4574 4574
Size: 5845
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5845
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2402 1184
Size: 1218 4627
AlwNI CAG_nnn'CTG
Cuts at: 3588 5160 3588
Size: 1572 4273
ApaLI G'TGCA_C
Cuts at: 3112 3902 5058 3112
Size: 790 1156 3899
Fragments arranged by size:
3899 1156 790
ApoI r'AATT_y
Cuts at: 1493 3154 3380 3532 5641 1493
Size: 1661 226 152 2109 1697
Fragments arranged by size:
2109 1697 1661 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4399 160
Size: 872 3367 1606
Fragments arranged by size:
3367 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5845
AvaI C'yCGr_G
Cuts at: 1870 1943 3170 1870
Size: 73 1227 4545
Fragments arranged by size:
4545 1227 73
BamHI G'GATC_C
Cuts at: 3175 3542 3175
Size: 367 5478
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3166 4621 618
Size: 325 217 627 1379 1455 1842
Fragments arranged by size:
1842 1455 1379 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2633 3015 3164 1391
Size: 1242 382 149 4072
Fragments arranged by size:
4072 1242 382 149
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2061 2303 2759 928
Size: 971 162 242 456 4014
Fragments arranged by size:
4014 971 456 242 162
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3103 3137 4036 4070 1903
Size: 34 1166 34 899 34 3678
Fragments arranged by size:
3678 1166 899 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3737 4947 3737
Size: 1210 4635
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5845
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2472 4456 136
Size: 107 122 71 631 605 800 1984 1525
Fragments arranged by size:
1984 1525 800 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5840 5840
Size: 5845
BlpI GC'TnA_GC
Cuts at: 3262 3262
Size: 5845
Bme1580I G_kGCm'C
Cuts at: 1790 2963 3116 3906 5062 1790
Size: 1173 153 790 1156 2573
Fragments arranged by size:
2573 1173 1156 790 153
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5845
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3576 4534 442
Size: 416 53 83 399 24 2159 958 1753
Fragments arranged by size:
2159 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5845
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4505 1239
Size: 3266 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2217 3970 4835 5133 2217
Size: 1753 865 298 2929
Fragments arranged by size:
2929 1753 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4508 882
Size: 617 3009 2219
Fragments arranged by size:
3009 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3636 493
Size: 711 2432 2702
Fragments arranged by size:
2702 2432 711
BsaBI GATnn'nnATC
Cuts at: 5839 5839
Size: 5845
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2598 2987 3652 4034
Size: 53 83 186 936 1065 389 665 382
Cuts at: 4034 4669 275
Size: 635 1451
Fragments arranged by size:
1451 1065 936 665 635 389 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4278 4676 4950 5097 5508 4278
Size: 398 274 147 411 4615
Fragments arranged by size:
4615 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5620
Fragments arranged by size:
5620 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5845
BseYI C'CCAG_C
Cuts at: 2915 2956 5048 5544 2915
Size: 41 2092 496 3216
Fragments arranged by size:
3216 2092 496 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2648 3074 1163
Size: 573 912 426 3934
Fragments arranged by size:
3934 912 573 426
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2683 3028 3295 4056 5084 1540
Size: 275 186 682 345 267 761 1028 2301
Fragments arranged by size:
2301 1028 761 682 345 275 267 186
BsiHKAI G_wGCw'C
Cuts at: 2200 2647 2948 3015 3116 3906 3991 5062 2200
Size: 447 301 67 101 790 85 1071 2983
Fragments arranged by size:
2983 1071 790 447 301 101 85 67
BsmI GAATG_Cn'
Cuts at: 2318 3356 3449 2318
Size: 1038 93 4714
Fragments arranged by size:
4714 1038 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2399 2399
Size: 5845
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2200 2633 2647 2948 2963 3015 3116
Size: 399 410 433 14 301 15 52 101
Cuts at: 3116 3164 3906 3991 5062 1391
Size: 48 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 433 410 399 301 101
85 52 48 15 14
BspEI T'CCGG_A
Cuts at: 5508 5508
Size: 5845
BspHI T'CATG_A
Cuts at: 2655 3734 5464 2655
Size: 1079 1730 3036
Fragments arranged by size:
3036 1730 1079
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2495 2529 2670 3200 844
Size: 1651 34 141 530 3489
Fragments arranged by size:
3489 1651 530 141 34
BsrBI CCG'CTC
Cuts at: 1394 1823 2608 3732 1394
Size: 429 785 1124 3507
Fragments arranged by size:
3507 1124 785 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2070 2944 4340 4514 65
Size: 1183 822 874 1396 174 1396
Fragments arranged by size:
1396 1396 1183 874 822 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2678 2948 4489 4676 1700
Size: 110 868 270 1541 187 2869
Fragments arranged by size:
2869 1541 868 270 187 110
BsrGI T'GTAC_A
Cuts at: 96 2694 96
Size: 2598 3247
BssHII G'CGCG_C
Cuts at: 1925 2507 1925
Size: 582 5263
BssSI C'ACGA_G
Cuts at: 3905 4917 3905
Size: 1012 4833
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3111 794
Size: 2317 3528
BstBI TT'CG_AA
Cuts at: 4649 4649
Size: 5845
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5845
BstYI r'GATC_y
Cuts at: 1972 3175 3542 3927 3944 5396 5482 5494 5840
Size: 1203 367 385 17 1452 86 12 346
Cuts at: 5840 1972
Size: 1977
Fragments arranged by size:
1977 1452 1203 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2525 2725 3028 513
Size: 552 1460 200 303 3330
Fragments arranged by size:
3330 1460 552 303 200
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5106
Fragments arranged by size:
5106 683 56
BtsI GCAGTG_nn'
Cuts at: 3070 3361 4155 4175 3070
Size: 291 794 20 4740
Fragments arranged by size:
4740 794 291 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5845
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5810
DraI TTT'AAA
Cuts at: 3149 3149
Size: 5845
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4852 809
Size: 507 3536 1802
Fragments arranged by size:
3536 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2094 2680 2826
Size: 54 1425 50 275 18 264 586 146
Cuts at: 2826 3025 3292 4181 5810 8
Size: 199 267 889 1629 43
Fragments arranged by size:
1629 1425 889 586 275 267 264 199
146 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2680 3025 3292 1537
Size: 275 868 345 267 4090
Fragments arranged by size:
4090 868 345 275 267
EarI CTCTTCn'nnn_
Cuts at: 1353 3775 1353
Size: 2422 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4432 4806 4952 387
Size: 1064 138 2843 374 146 1280
Fragments arranged by size:
2843 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3013 3013
Size: 5845
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2771 2822 3908 4505 5292 5560 1239
Size: 393 1139 51 1086 597 787 268 1524
Fragments arranged by size:
1524 1139 1086 787 597 393 268 51
EcoO109I rG'GnC_Cy
Cuts at: 2518 2518
Size: 5845
EcoRI G'AATT_C
Cuts at: 3154 3532 3154
Size: 378 5467
FseI GG_CCGG'CC
Cuts at: 2682 2682
Size: 5845
FspI TGC'GCA
Cuts at: 2936 4351 2936
Size: 1415 4430
HaeII r_GCGC'y
Cuts at: 1678 3067 4992 1678
Size: 1389 1925 2531
Fragments arranged by size:
2531 1925 1389
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2351 2383 4486 4518 4560
Size: 32 135 32 779 32 2103 32 42
Cuts at: 4560 4592 1373
Size: 32 2626
Fragments arranged by size:
2626 2103 779 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2866 3189 3435 669
Size: 988 281 928 323 246 3079
Fragments arranged by size:
3079 988 928 323 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5845
HpaI GTT'AAC
Cuts at: 3435 3435
Size: 5845
KpnI G_GTAC'C
Cuts at: 3170 3170
Size: 5845
MfeI C'AATT_G
Cuts at: 3444 3444
Size: 5845
MluI A'CGCG_T
Cuts at: 5721 5721
Size: 5845
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2353 2970 4959 5143 1305
Size: 1048 617 1989 184 2007
Fragments arranged by size:
2007 1989 1048 617 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2096 10
Size: 54 1425 343 264 3759
Fragments arranged by size:
3759 1425 343 264 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2654 3803 4162 4321 518
Size: 648 1488 1149 359 159 2042
Fragments arranged by size:
2042 1488 1149 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2527 2770 3261 3938 5086 5331 1606
Size: 921 243 491 677 1148 245 2120
Fragments arranged by size:
2120 1148 921 677 491 245 243
NaeI GCC'GGC
Cuts at: 2680 2680
Size: 5845
NcoI C'CATG_G
Cuts at: 513 1065 2725 513
Size: 552 1660 3633
Fragments arranged by size:
3633 1660 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5845
NgoMIV G'CCGG_C
Cuts at: 2678 2678
Size: 5845
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5845
NotI GC'GGCC_GC
Cuts at: 3292 3292
Size: 5845
NspI r_CATG'y
Cuts at: 1132 1229 2275 2760 3203 4748 1132
Size: 97 1046 485 443 1545 2229
Fragments arranged by size:
2229 1545 1046 485 443 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5845
PciI A'CATG_T
Cuts at: 4744 4744
Size: 5845
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2139 1108
Size: 1031 4814
PmeI GTTT'AAAC
Cuts at: 3149 3149
Size: 5845
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3914 3946 5427 5459 3914
Size: 32 1481 32 4300
Fragments arranged by size:
4300 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2518 2518
Size: 5845
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5845
PsiI TTA'TAA
Cuts at: 3415 3415
Size: 5845
PstI C_TGCA'G
Cuts at: 830 2213 2351 2498 2769 3197 830
Size: 1383 138 147 271 428 3478
Fragments arranged by size:
3478 1383 428 271 147 138
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5845
PvuII CAG'CTG
Cuts at: 2770 2770
Size: 5845
SacI G_AGCT'C
Cuts at: 3015 3015
Size: 5845
SacII CC_GC'GG
Cuts at: 2528 2528
Size: 5845
SalI G'TCGA_C
Cuts at: 1936 3187 1936
Size: 1251 4594
SbfI CC_TGCA'GG
Cuts at: 3197 3197
Size: 5845
ScaI AGT'ACT
Cuts at: 4093 5805 4093
Size: 1712 4133
SexAI A'CCwGG_T
Cuts at: 2898 2898
Size: 5845
SfcI C'TryA_G
Cuts at: 826 1045 2209 2347 2494 2765 3193 4328 5009
Size: 219 1164 138 147 271 428 1135 681
Cuts at: 5009 5200 826
Size: 191 1471
Fragments arranged by size:
1471 1164 1135 681 428 271 219 191
147 138
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5845
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5845
SmaI CCC'GGG
Cuts at: 1872 3172 1872
Size: 1300 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2232 3949 4850 5112 820
Size: 197 926 289 1717 901 262 1553
Fragments arranged by size:
1717 1553 926 901 289 262 197
SnaBI TAC'GTA
Cuts at: 493 3636 493
Size: 3143 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5845
SphI G_CATG'C
Cuts at: 1132 3203 1132
Size: 2071 3774
SspI AAT'ATT
Cuts at: 5 52 2025 3769 5
Size: 47 1973 1744 2081
Fragments arranged by size:
2081 1973 1744 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5845
StyI C'CwwG_G
Cuts at: 513 1065 2514 2583 2625 2725 3284 513
Size: 552 1449 69 42 100 559 3074
Fragments arranged by size:
3074 1449 559 552 100 69 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2780 3872 3889 4042 4227 4672 325
Size: 1255 1200 1092 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1200 1092 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2277 2694 4091
Size: 274 80 33 51 1443 300 417 1397
Cuts at: 4091 5803 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1397 417 300 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5805 5837 5805
Size: 32 5813
Tth111I GACn'n_nGTC
Cuts at: 1316 2900 1316
Size: 1584 4261
XbaI T'CTAG_A
Cuts at: 3181 3181
Size: 5845
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2590 2896 2590
Size: 306 5539
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5845
XmaI C'CCGG_G
Cuts at: 1870 3170 1870
Size: 1300 4545
XmnI GAAnn'nnTTC
Cuts at: 3974 3974
Size: 5845
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2988 3653 276
Size: 53 83 186 936 1454 665 2468
Fragments arranged by size:
2468 1454 936 665 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SacII SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoRV FalI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SanDI SapI
SfiI SfoI SrfI SwaI