Restriction map for
pFN21AE1860
(Circular) (Six-base) MAPSORT of: pFN21AE1860.seq Check: 6052 from: 1 to: 5845
pFN21AE1860 5845 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2170 2500 2170
Size: 330 5515
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3655 278
Size: 53 83 186 936 2119 2468
Fragments arranged by size:
2468 2119 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3188 1937
Size: 1251 4594
Acc65I G'GTAC_C
Cuts at: 3166 3166
Size: 5845
AclI AA'CG_TT
Cuts at: 1563 3972 4345 1563
Size: 2409 373 3063
Fragments arranged by size:
3063 2409 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2086 3908 5292 5560 1632
Size: 454 1822 1384 268 1917
Fragments arranged by size:
1917 1822 1384 454 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5648
AflIII A'CryG_T
Cuts at: 1203 4744 5721 1203
Size: 3541 977 1327
Fragments arranged by size:
3541 1327 977
AgeI A'CCGG_T
Cuts at: 4676 4676
Size: 5845
AhdI GACnn_n'nnGTC
Cuts at: 4574 4574
Size: 5845
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5845
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2969 1184
Size: 1785 4060
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2387 2419 3121 3153 2387
Size: 32 702 32 5079
Fragments arranged by size:
5079 702 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3124 3588 5160 3124
Size: 464 1572 3809
Fragments arranged by size:
3809 1572 464
ApaI G_GGCC'C
Cuts at: 2358 2651 2674 2748 2358
Size: 293 23 74 5455
Fragments arranged by size:
5455 293 74 23
ApaLI G'TGCA_C
Cuts at: 3902 5058 3902
Size: 1156 4689
ApoI r'AATT_y
Cuts at: 1493 3154 3380 3532 5641 1493
Size: 1661 226 152 2109 1697
Fragments arranged by size:
2109 1697 1661 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4399 160
Size: 872 3367 1606
Fragments arranged by size:
3367 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5845
AvaI C'yCGr_G
Cuts at: 1870 1943 3170 1870
Size: 73 1227 4545
Fragments arranged by size:
4545 1227 73
BamHI G'GATC_C
Cuts at: 3175 3542 3175
Size: 367 5478
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3166 4621 618
Size: 325 217 627 1379 1455 1842
Fragments arranged by size:
1842 1455 1379 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2358 2651 2674 2748 3164 1391
Size: 967 293 23 74 416 4072
Fragments arranged by size:
4072 967 416 293 74 23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2964 928
Size: 971 1065 3809
Fragments arranged by size:
3809 1065 971
BbvCI CC'TCA_GC
Cuts at: 3125 3125
Size: 5845
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4036 4070 1903
Size: 34 2099 34 3678
Fragments arranged by size:
3678 2099 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3737 4947 3737
Size: 1210 4635
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5845
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2321 2336 2816
Size: 107 122 71 631 605 649 15 480
Cuts at: 2816 4456 136
Size: 1640 1525
Fragments arranged by size:
1640 1525 649 631 605 480 122 107
71 15
BglII A'GATC_T
Cuts at: 2115 5840 2115
Size: 3725 2120
BlpI GC'TnA_GC
Cuts at: 3262 3262
Size: 5845
Bme1580I G_kGCm'C
Cuts at: 1790 2332 2358 2651 2674 2748 3906 5062 1790
Size: 542 26 293 23 74 1158 1156 2573
Fragments arranged by size:
2573 1158 1156 542 293 74 26 23
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5845
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2299 3576 4534
Size: 416 53 83 399 24 882 1277 958
Cuts at: 4534 442
Size: 1753
Fragments arranged by size:
1753 1277 958 882 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5845
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2723 2752 2723
Size: 29 5816
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2526 2718 3001 4505 1239
Size: 1287 192 283 1504 2579
Fragments arranged by size:
2579 1504 1287 283 192
Bpu10I CC'TnA_GC
Cuts at: 3125 3125
Size: 5845
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3970 4835 5133 3970
Size: 865 298 4682
Fragments arranged by size:
4682 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2476 2920 2987 4508 882
Size: 617 977 444 67 1521 2219
Fragments arranged by size:
2219 1521 977 617 444 67
BsaAI yAC'GTr
Cuts at: 493 1204 3636 493
Size: 711 2432 2702
Fragments arranged by size:
2702 2432 711
BsaBI GATnn'nnATC
Cuts at: 5839 5839
Size: 5845
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3652 4034 4669 275
Size: 53 83 186 936 2119 382 635 1451
Fragments arranged by size:
2119 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4278 4676 4950 5097 5508 4278
Size: 398 274 147 411 4615
Fragments arranged by size:
4615 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2387 2417 2616 2646 1178
Size: 30 165 30 984 30 199 30 4377
Fragments arranged by size:
4377 984 199 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2426 2529 2744 2897 1186
Size: 1240 103 215 153 4134
Fragments arranged by size:
4134 1240 215 153 103
BseYI C'CCAG_C
Cuts at: 2368 2504 2584 2666 3011 5048 5544 2368
Size: 136 80 82 345 2037 496 2669
Fragments arranged by size:
2669 2037 496 345 136 82 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2607 2989 1163
Size: 573 871 382 4019
Fragments arranged by size:
4019 871 573 382
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3295 4056 5084 1540
Size: 275 186 1294 761 1028 2301
Fragments arranged by size:
2301 1294 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2457 3906 3991 5062 2457
Size: 1449 85 1071 3240
Fragments arranged by size:
3240 1449 1071 85
BsmI GAATG_Cn'
Cuts at: 3356 3449 3356
Size: 93 5752
BsmBI CGTCTCn'nnnn_
Cuts at: 2006 2006
Size: 5845
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2332 2358 2457 2651 2674 2748 3164
Size: 399 542 26 99 194 23 74 416
Cuts at: 3164 3906 3991 5062 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 542 416 399 194 99
85 74 26 23
BspEI T'CCGG_A
Cuts at: 5508 5508
Size: 5845
BspHI T'CATG_A
Cuts at: 3734 5464 3734
Size: 1730 4115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2170 2500 3200 844
Size: 1326 330 700 3489
Fragments arranged by size:
3489 1326 700 330
BsrBI CCG'CTC
Cuts at: 1394 1823 3732 1394
Size: 429 1909 3507
Fragments arranged by size:
3507 1909 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4340 4514 65
Size: 1183 3092 174 1396
Fragments arranged by size:
3092 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2315 4489 4676 1700
Size: 110 505 2174 187 2869
Fragments arranged by size:
2869 2174 505 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5845
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5845
BssSI C'ACGA_G
Cuts at: 3905 4917 3905
Size: 1012 4833
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2693 794
Size: 1899 3946
BstBI TT'CG_AA
Cuts at: 4649 4649
Size: 5845
BstEII G'GTnAC_C
Cuts at: 1184 2980 1184
Size: 1796 4049
BstYI r'GATC_y
Cuts at: 1972 2115 3175 3542 3927 3944 5396 5482 5494
Size: 143 1060 367 385 17 1452 86 12
Cuts at: 5494 5840 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1060 385 367 346 143 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2737 2737
Size: 5845
BtgI C'CryG_G
Cuts at: 513 1065 2130 513
Size: 552 1065 4228
Fragments arranged by size:
4228 1065 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2083 487
Size: 683 56 857 4249
Fragments arranged by size:
4249 857 683 56
BtsI GCAGTG_nn'
Cuts at: 3361 4155 4175 3361
Size: 794 20 5031
Fragments arranged by size:
5031 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5845
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2824 2859 529
Size: 35 2260 35 3515
Fragments arranged by size:
3515 2260 35 35
DraI TTT'AAA
Cuts at: 3149 3149
Size: 5845
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4852 809
Size: 507 3536 1802
Fragments arranged by size:
3536 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2133 2322 2682
Size: 54 1425 50 275 18 303 189 360
Cuts at: 2682 3292 4181 5810 8
Size: 610 889 1629 43
Fragments arranged by size:
1629 1425 889 610 360 303 275 189
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3292 1537
Size: 275 1480 4090
Fragments arranged by size:
4090 1480 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2793 3057 3775 1353
Size: 1440 264 718 3423
Fragments arranged by size:
3423 1440 718 264
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4432 4806 4952 387
Size: 1064 138 2843 374 146 1280
Fragments arranged by size:
2843 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2086 2526 2718 3001 3908 4505 5292
Size: 393 454 440 192 283 907 597 787
Cuts at: 5292 5560 1239
Size: 268 1524
Fragments arranged by size:
1524 907 787 597 454 440 393 283
268 192
EcoNI CCTnn'n_nnAGG
Cuts at: 2541 2956 2541
Size: 415 5430
EcoO109I rG'GnC_Cy
Cuts at: 2090 2272 2355 2528 2546 2581 2647 2648 2670
Size: 182 83 173 18 35 66 1 22
Cuts at: 2670 2671 2744 2090
Size: 1 73 5191
Fragments arranged by size:
5191 182 173 83 73 66 35 22
18 1 1
EcoRI G'AATT_C
Cuts at: 3154 3532 3154
Size: 378 5467
FspI TGC'GCA
Cuts at: 4351 4351
Size: 5845
HaeII r_GCGC'y
Cuts at: 1678 2467 4992 1678
Size: 789 2525 2531
Fragments arranged by size:
2531 2525 789
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4486 4518 4560 4592 1373
Size: 32 135 32 2914 32 42 32 2626
Fragments arranged by size:
2914 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3189 3435 669
Size: 988 281 1251 246 3079
Fragments arranged by size:
3079 1251 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5845
HpaI GTT'AAC
Cuts at: 3435 3435
Size: 5845
KpnI G_GTAC'C
Cuts at: 3170 3170
Size: 5845
MfeI C'AATT_G
Cuts at: 3444 3444
Size: 5845
MluI A'CGCG_T
Cuts at: 5721 5721
Size: 5845
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2322 2430 4959 5143 1305
Size: 1017 108 2529 184 2007
Fragments arranged by size:
2529 2007 1017 184 108
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2324 10
Size: 54 1425 343 492 3531
Fragments arranged by size:
3531 1425 492 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2042 3803 4162 4321 518
Size: 648 876 1761 359 159 2042
Fragments arranged by size:
2042 1761 876 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2504 2588 3261 3938 5086 5331 1606
Size: 898 84 673 677 1148 245 2120
Fragments arranged by size:
2120 1148 898 677 673 245 84
NaeI GCC'GGC
Cuts at: 2317 2317
Size: 5845
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5293
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5845
NgoMIV G'CCGG_C
Cuts at: 2315 2315
Size: 5845
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5845
NotI GC'GGCC_GC
Cuts at: 3292 3292
Size: 5845
NspI r_CATG'y
Cuts at: 1132 1229 3203 4748 1132
Size: 97 1974 1545 2229
Fragments arranged by size:
2229 1974 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2931 1348
Size: 1583 4262
PciI A'CATG_T
Cuts at: 4744 4744
Size: 5845
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2182 1108
Size: 1074 4771
PfoI T'CCnGG_A
Cuts at: 3117 3117
Size: 5845
PmeI GTTT'AAAC
Cuts at: 3149 3149
Size: 5845
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2387 2419 3914 3946 5427 5459 2387
Size: 32 1495 32 1481 32 2773
Fragments arranged by size:
2773 1495 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2090 2528 2546 2581 2090
Size: 438 18 35 5354
Fragments arranged by size:
5354 438 35 18
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5845
PsiI TTA'TAA
Cuts at: 3415 3415
Size: 5845
PspOMI G'GGCC_C
Cuts at: 2354 2647 2670 2744 2354
Size: 293 23 74 5455
Fragments arranged by size:
5455 293 74 23
PstI C_TGCA'G
Cuts at: 830 2593 3197 830
Size: 1763 604 3478
Fragments arranged by size:
3478 1763 604
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5845
PvuII CAG'CTG
Cuts at: 2588 2588
Size: 5845
SalI G'TCGA_C
Cuts at: 1936 3187 1936
Size: 1251 4594
SanDI GG'GwC_CC
Cuts at: 2090 2581 2090
Size: 491 5354
SbfI CC_TGCA'GG
Cuts at: 3197 3197
Size: 5845
ScaI AGT'ACT
Cuts at: 4093 5805 4093
Size: 1712 4133
SexAI A'CCwGG_T
Cuts at: 2381 2381
Size: 5845
SfcI C'TryA_G
Cuts at: 826 1045 2589 3035 3193 4328 5009 5200 826
Size: 219 1544 446 158 1135 681 191 1471
Fragments arranged by size:
1544 1471 1135 681 446 219 191 158
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5845
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5845
SmaI CCC'GGG
Cuts at: 1872 3172 1872
Size: 1300 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3949 4850 5112 820
Size: 197 926 2006 901 262 1553
Fragments arranged by size:
2006 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3636 493
Size: 3143 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5845
SphI G_CATG'C
Cuts at: 1132 3203 1132
Size: 2071 3774
SspI AAT'ATT
Cuts at: 5 52 3769 5
Size: 47 3717 2081
Fragments arranged by size:
3717 2081 47
StuI AGG'CCT
Cuts at: 1517 2099 2679 2735 1517
Size: 582 580 56 4627
Fragments arranged by size:
4627 582 580 56
StyI C'CwwG_G
Cuts at: 513 1065 2859 2957 3284 513
Size: 552 1794 98 327 3074
Fragments arranged by size:
3074 1794 552 327 98
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2377 3872 3889 4042 4227 4672 325
Size: 1255 797 1495 17 153 185 445 1498
Fragments arranged by size:
1498 1495 1255 797 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4091 5803 96
Size: 274 80 33 51 1443 2114 1712 138
Fragments arranged by size:
2114 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2616 2648 5805 5837 2616
Size: 32 3157 32 2624
Fragments arranged by size:
3157 2624 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5845
XbaI T'CTAG_A
Cuts at: 3181 3181
Size: 5845
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5845
XmaI C'CCGG_G
Cuts at: 1870 3170 1870
Size: 1300 4545
XmnI GAAnn'nnTTC
Cuts at: 3974 3974
Size: 5845
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3653 276
Size: 53 83 186 936 2119 2468
Fragments arranged by size:
2468 2119 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstXI
BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PmlI PspXI PsrI RsrII
SacI SacII SapI SfiI SfoI SrfI SwaI XcmI