Restriction map for
pFN21AE1855
(Circular) (Six-base) MAPSORT of: pFN21AE1855.seq Check: 8403 from: 1 to: 5824
pFN21AE1855 5824 bp
With 182 enzymes: SgfI *
August 16, 2010 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3634 278
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2950 3167 1937
Size: 1013 217 4594
Fragments arranged by size:
4594 1013 217
Acc65I G'GTAC_C
Cuts at: 3015 3145 3015
Size: 130 5694
AclI AA'CG_TT
Cuts at: 1563 3951 4324 1563
Size: 2388 373 3063
Fragments arranged by size:
3063 2388 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2642 3887 5271 5539 1632
Size: 1010 1245 1384 268 1917
Fragments arranged by size:
1917 1384 1245 1010 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5627
AflIII A'CryG_T
Cuts at: 1203 3121 4723 5700 1203
Size: 1918 1602 977 1327
Fragments arranged by size:
1918 1602 1327 977
AgeI A'CCGG_T
Cuts at: 4655 4655
Size: 5824
AhdI GACnn_n'nnGTC
Cuts at: 2679 2782 4553 2679
Size: 103 1771 3950
Fragments arranged by size:
3950 1771 103
AleI CACnn'nnGTG
Cuts at: 1166 2228 1166
Size: 1062 4762
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5824
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3055 3087 3055
Size: 32 5792
AlwNI CAG_nnn'CTG
Cuts at: 2198 2299 3105 3567 5139 2198
Size: 101 806 462 1572 2883
Fragments arranged by size:
2883 1572 806 462 101
ApaLI G'TGCA_C
Cuts at: 2417 3881 5037 2417
Size: 1464 1156 3204
Fragments arranged by size:
3204 1464 1156
ApoI r'AATT_y
Cuts at: 1493 3133 3359 3511 5620 1493
Size: 1640 226 152 2109 1697
Fragments arranged by size:
2109 1697 1640 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4378 160
Size: 872 3346 1606
Fragments arranged by size:
3346 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
AvaI C'yCGr_G
Cuts at: 1870 1943 3149 1870
Size: 73 1206 4545
Fragments arranged by size:
4545 1206 73
BamHI G'GATC_C
Cuts at: 3154 3521 3154
Size: 367 5457
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2849 2936 3015 3145 4600
Size: 325 217 627 1062 87 79 130 1455
Cuts at: 4600 618
Size: 1842
Fragments arranged by size:
1842 1455 1062 627 325 217 130 87
79
BanII G_rGCy'C
Cuts at: 1391 2103 3143 1391
Size: 712 1040 4072
Fragments arranged by size:
4072 1040 712
BbeI G_GCGC'C
Cuts at: 2853 2853
Size: 5824
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2017 2618 928
Size: 971 118 601 4134
Fragments arranged by size:
4134 971 601 118
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2894 2928 4015 4049 1903
Size: 34 50 34 873 34 1087 34 3678
Fragments arranged by size:
3678 1087 873 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3716 4926 3716
Size: 1210 4614
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5824
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2504 2847 4435
Size: 107 122 71 631 605 832 343 1588
Cuts at: 4435 136
Size: 1525
Fragments arranged by size:
1588 1525 832 631 605 343 122 107
71
BglII A'GATC_T
Cuts at: 5819 5819
Size: 5824
BlpI GC'TnA_GC
Cuts at: 3241 3241
Size: 5824
Bme1580I G_kGCm'C
Cuts at: 1790 2421 2939 3885 5041 1790
Size: 631 518 946 1156 2573
Fragments arranged by size:
2573 1156 946 631 518
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5824
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3555 4513 442
Size: 416 53 83 399 24 2138 958 1753
Fragments arranged by size:
2138 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5824
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2140 2140
Size: 5824
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2027 2087 2285 4484 1239
Size: 788 60 198 2199 2579
Fragments arranged by size:
2579 2199 788 198 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2789 3949 4814 5112 2789
Size: 1160 865 298 3501
Fragments arranged by size:
3501 1160 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2045 2176 2666 4487 882
Size: 617 546 131 490 1821 2219
Fragments arranged by size:
2219 1821 617 546 490 131
BsaAI yAC'GTr
Cuts at: 493 1204 3615 493
Size: 711 2411 2702
Fragments arranged by size:
2702 2411 711
BsaBI GATnn'nnATC
Cuts at: 5818 5818
Size: 5824
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2850 3631 4013 4648
Size: 53 83 186 936 1317 781 382 635
Cuts at: 4648 275
Size: 1451
Fragments arranged by size:
1451 1317 936 781 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4257 4655 4929 5076 5487 4257
Size: 398 274 147 411 4594
Fragments arranged by size:
4594 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2426 2456 3055 3085 1178
Size: 30 165 30 1023 30 599 30 3917
Fragments arranged by size:
3917 1023 599 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2878 1186
Size: 1692 4132
BseYI C'CCAG_C
Cuts at: 2127 2486 2698 5027 5523 2127
Size: 359 212 2329 496 2428
Fragments arranged by size:
2428 2329 496 359 212
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5251
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3274 4035 5063 1540
Size: 275 186 1273 761 1028 2301
Fragments arranged by size:
2301 1273 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2421 3885 3970 5041 2421
Size: 1464 85 1071 3204
Fragments arranged by size:
3204 1464 1071 85
BsmI GAATG_Cn'
Cuts at: 3335 3428 3335
Size: 93 5731
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2103 2421 2939 3143 3885 3970 5041
Size: 399 313 318 518 204 742 85 1071
Cuts at: 5041 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 518 399 318 313 204
85
BspEI T'CCGG_A
Cuts at: 5487 5487
Size: 5824
BspHI T'CATG_A
Cuts at: 3713 5443 3713
Size: 1730 4094
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2747 3042 3179 844
Size: 1903 295 137 3489
Fragments arranged by size:
3489 1903 295 137
BsrBI CCG'CTC
Cuts at: 1394 1823 3711 1394
Size: 429 1888 3507
Fragments arranged by size:
3507 1888 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2910 4319 4493 65
Size: 1183 1662 1409 174 1396
Fragments arranged by size:
1662 1409 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4468 4655 1700
Size: 110 2658 187 2869
Fragments arranged by size:
2869 2658 187 110
BsrGI T'GTAC_A
Cuts at: 96 3087 96
Size: 2991 2833
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5824
BssSI C'ACGA_G
Cuts at: 3884 4896 3884
Size: 1012 4812
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5824
BstBI TT'CG_AA
Cuts at: 4628 4628
Size: 5824
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5824
BstXI CCAn_nnnn'nTGG
Cuts at: 2258 2258
Size: 5824
BstYI r'GATC_y
Cuts at: 1972 3154 3521 3906 3923 5375 5461 5473 5819
Size: 1182 367 385 17 1452 86 12 346
Cuts at: 5819 1972
Size: 1977
Fragments arranged by size:
1977 1452 1182 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2951 2951
Size: 5824
BtgI C'CryG_G
Cuts at: 513 1065 2012 2166 2508 2648 3003 513
Size: 552 947 154 342 140 355 3334
Fragments arranged by size:
3334 947 552 355 342 154 140
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5085
Fragments arranged by size:
5085 683 56
BtsI GCAGTG_nn'
Cuts at: 2724 3340 4134 4154 2724
Size: 616 794 20 4394
Fragments arranged by size:
4394 794 616 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5824
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2317 2352 529
Size: 35 1753 35 4001
Fragments arranged by size:
4001 1753 35 35
DraI TTT'AAA
Cuts at: 3128 3128
Size: 5824
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2268 2282 4831 809
Size: 507 952 14 2549 1802
Fragments arranged by size:
2549 1802 952 507 14
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2505 3271 4160
Size: 54 1425 50 275 18 675 766 889
Cuts at: 4160 5789 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 766 675 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3271 1537
Size: 275 1459 4090
Fragments arranged by size:
4090 1459 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3754 1353
Size: 2401 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4411 4785 4931 387
Size: 1064 138 2822 374 146 1280
Fragments arranged by size:
2822 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2027 2087 2285 2642 3887 4484 5271
Size: 393 395 60 198 357 1245 597 787
Cuts at: 5271 5539 1239
Size: 268 1524
Fragments arranged by size:
1524 1245 787 597 395 393 357 268
198 60
EcoNI CCTnn'n_nnAGG
Cuts at: 2624 2624
Size: 5824
EcoO109I rG'GnC_Cy
Cuts at: 2736 2736
Size: 5824
EcoRI G'AATT_C
Cuts at: 3133 3511 3133
Size: 378 5446
FspI TGC'GCA
Cuts at: 4330 4330
Size: 5824
HaeII r_GCGC'y
Cuts at: 1678 2853 4971 1678
Size: 1175 2118 2531
Fragments arranged by size:
2531 2118 1175
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2665 2697 2768 2800 4465
Size: 32 135 32 1093 32 71 32 1665
Cuts at: 4465 4497 4539 4571 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1665 1093 135 71 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3168 3414 669
Size: 988 281 1230 246 3079
Fragments arranged by size:
3079 1230 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5824
HpaI GTT'AAC
Cuts at: 3414 3414
Size: 5824
KasI G'GCGC_C
Cuts at: 2849 2849
Size: 5824
KpnI G_GTAC'C
Cuts at: 3019 3149 3019
Size: 130 5694
MfeI C'AATT_G
Cuts at: 3423 3423
Size: 5824
MluI A'CGCG_T
Cuts at: 5700 5700
Size: 5824
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4938 5122 1305
Size: 3633 184 2007
Fragments arranged by size:
3633 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4002
Fragments arranged by size:
4002 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2228 3051 3782 4141 4300 518
Size: 648 1062 823 731 359 159 2042
Fragments arranged by size:
2042 1062 823 731 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2218 3240 3917 5065 5310 1606
Size: 612 1022 677 1148 245 2120
Fragments arranged by size:
2120 1148 1022 677 612 245
NarI GG'CG_CC
Cuts at: 2850 2850
Size: 5824
NcoI C'CATG_G
Cuts at: 513 1065 2508 3003 513
Size: 552 1443 495 3334
Fragments arranged by size:
3334 1443 552 495
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5824
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5824
NotI GC'GGCC_GC
Cuts at: 3271 3271
Size: 5824
NspI r_CATG'y
Cuts at: 1132 1229 3182 4727 1132
Size: 97 1953 1545 2229
Fragments arranged by size:
2229 1953 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2446 2917 1348
Size: 1098 471 4255
Fragments arranged by size:
4255 1098 471
PciI A'CATG_T
Cuts at: 4723 4723
Size: 5824
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5824
PfoI T'CCnGG_A
Cuts at: 2063 2772 2063
Size: 709 5115
PmeI GTTT'AAAC
Cuts at: 3128 3128
Size: 5824
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3055 3087 3893 3925 5406 5438 3055
Size: 32 806 32 1481 32 3441
Fragments arranged by size:
3441 1481 806 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2736 2736
Size: 5824
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5824
PsiI TTA'TAA
Cuts at: 2826 3394 2826
Size: 568 5256
PstI C_TGCA'G
Cuts at: 830 2210 2291 2313 2968 3176 830
Size: 1380 81 22 655 208 3478
Fragments arranged by size:
3478 1380 655 208 81 22
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5824
PvuII CAG'CTG
Cuts at: 2218 2218
Size: 5824
SalI G'TCGA_C
Cuts at: 1936 3166 1936
Size: 1230 4594
SbfI CC_TGCA'GG
Cuts at: 3176 3176
Size: 5824
ScaI AGT'ACT
Cuts at: 4072 5784 4072
Size: 1712 4112
SfcI C'TryA_G
Cuts at: 826 1045 2206 2287 2309 2964 3172 4307 4988
Size: 219 1161 81 22 655 208 1135 681
Cuts at: 4988 5179 826
Size: 191 1471
Fragments arranged by size:
1471 1161 1135 681 655 219 208 191
81 22
SfoI GGC'GCC
Cuts at: 2851 2851
Size: 5824
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5824
SmaI CCC'GGG
Cuts at: 1872 3151 1872
Size: 1279 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2804 3928 4829 5091 820
Size: 197 926 861 1124 901 262 1553
Fragments arranged by size:
1553 1124 926 901 861 262 197
SnaBI TAC'GTA
Cuts at: 493 3615 493
Size: 3122 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5824
SphI G_CATG'C
Cuts at: 1132 3182 1132
Size: 2050 3774
SspI AAT'ATT
Cuts at: 5 52 3748 5
Size: 47 3696 2081
Fragments arranged by size:
3696 2081 47
StuI AGG'CCT
Cuts at: 1517 2048 2145 1517
Size: 531 97 5196
Fragments arranged by size:
5196 531 97
StyI C'CwwG_G
Cuts at: 513 1065 2410 2425 2508 2881 3003 3263 513
Size: 552 1345 15 83 373 122 260 3074
Fragments arranged by size:
3074 1345 552 373 260 122 83 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2328 2438 2971 3851 3868 4021 4206
Size: 1255 748 110 533 880 17 153 185
Cuts at: 4206 4651 325
Size: 445 1498
Fragments arranged by size:
1498 1255 880 748 533 445 185 153
110 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3087 4070 5782
Size: 274 80 33 51 1443 1110 983 1712
Cuts at: 5782 96
Size: 138
Fragments arranged by size:
1712 1443 1110 983 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2537 2569 5784 5816 2537
Size: 32 3215 32 2545
Fragments arranged by size:
3215 2545 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5824
XbaI T'CTAG_A
Cuts at: 3160 3160
Size: 5824
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2333 2655 3034 2333
Size: 322 379 5123
Fragments arranged by size:
5123 379 322
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5824
XmaI C'CCGG_G
Cuts at: 1870 3149 1870
Size: 1279 4545
XmnI GAAnn'nnTTC
Cuts at: 3953 3953
Size: 5824
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3632 276
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SrfI SwaI