Restriction map for
pFN21AE1854
(Circular) (Six-base) MAPSORT of: pFN21AE1854.seq Check: 2402 from: 1 to: 5764
pFN21AE1854 5764 bp
With 182 enzymes: SgfI *
August 16, 2010 14:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2235 3574 278
Size: 53 83 186 936 699 1339 2468
Fragments arranged by size:
2468 1339 936 699 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3107 1937
Size: 1170 4594
Acc65I G'GTAC_C
Cuts at: 3085 3085
Size: 5764
AclI AA'CG_TT
Cuts at: 1563 3891 4264 1563
Size: 2328 373 3063
Fragments arranged by size:
3063 2328 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2171 3827 5211 5479 1632
Size: 539 1656 1384 268 1917
Fragments arranged by size:
1917 1656 1384 539 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5567
AflIII A'CryG_T
Cuts at: 1203 2604 2619 4663 5640 1203
Size: 1401 15 2044 977 1327
Fragments arranged by size:
2044 1401 1327 977 15
AgeI A'CCGG_T
Cuts at: 4595 4595
Size: 5764
AhdI GACnn_n'nnGTC
Cuts at: 4493 4493
Size: 5764
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5764
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5764
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2290 2322 2290
Size: 32 5732
AlwNI CAG_nnn'CTG
Cuts at: 3507 5079 3507
Size: 1572 4192
ApaI G_GGCC'C
Cuts at: 2284 2284
Size: 5764
ApaLI G'TGCA_C
Cuts at: 2792 3821 4977 2792
Size: 1029 1156 3579
Fragments arranged by size:
3579 1156 1029
ApoI r'AATT_y
Cuts at: 1493 3073 3299 3451 5560 1493
Size: 1580 226 152 2109 1697
Fragments arranged by size:
2109 1697 1580 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4318 160
Size: 872 3286 1606
Fragments arranged by size:
3286 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5764
AvaI C'yCGr_G
Cuts at: 1870 1943 2239 2490 2864 3089 1870
Size: 73 296 251 374 225 4545
Fragments arranged by size:
4545 374 296 251 225 73
BamHI G'GATC_C
Cuts at: 3094 3461 3094
Size: 367 5397
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2202 2243 2506 2948 3085
Size: 325 217 627 415 41 263 442 137
Cuts at: 3085 4540 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 442 415 325 263 217
137 41
BanII G_rGCy'C
Cuts at: 1391 2284 2403 2655 3083 1391
Size: 893 119 252 428 4072
Fragments arranged by size:
4072 893 428 252 119
BbeI G_GCGC'C
Cuts at: 2510 2510
Size: 5764
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2745 2933 928
Size: 971 846 188 3759
Fragments arranged by size:
3759 971 846 188
BbvCI CC'TCA_GC
Cuts at: 2082 2082
Size: 5764
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2275 2309 3955 3989 1903
Size: 34 338 34 1646 34 3678
Fragments arranged by size:
3678 1646 338 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3656 4866 3656
Size: 1210 4554
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5764
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2804 4375
Size: 107 122 71 631 605 337 795 1571
Cuts at: 4375 136
Size: 1525
Fragments arranged by size:
1571 1525 795 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 2940 5759 2940
Size: 2819 2945
BlpI GC'TnA_GC
Cuts at: 3181 3181
Size: 5764
Bme1580I G_kGCm'C
Cuts at: 1790 2246 2284 2796 3825 4981 1790
Size: 456 38 512 1029 1156 2573
Fragments arranged by size:
2573 1156 1029 512 456 38
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5764
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2399 3495 4453
Size: 416 53 83 399 24 982 1096 958
Cuts at: 4453 442
Size: 1753
Fragments arranged by size:
1753 1096 982 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4424 1239
Size: 3185 2579
Bpu10I CC'TnA_GC
Cuts at: 2082 2082
Size: 5764
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3889 4754 5052 3889
Size: 865 298 4601
Fragments arranged by size:
4601 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4427 882
Size: 617 2928 2219
Fragments arranged by size:
2928 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2362 2791 3555 493
Size: 711 1158 429 764 2702
Fragments arranged by size:
2702 1158 764 711 429
BsaBI GATnn'nnATC
Cuts at: 5758 5758
Size: 5764
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2232 2507 3571 3953
Size: 53 83 186 936 699 275 1064 382
Cuts at: 3953 4588 275
Size: 635 1451
Fragments arranged by size:
1451 1064 936 699 635 382 275 186
83 53
BsaWI w'CCGG_w
Cuts at: 3054 4197 4595 4869 5016 5427 3054
Size: 1143 398 274 147 411 3391
Fragments arranged by size:
3391 1143 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2099 2129 2168 2198 2591
Size: 30 165 30 696 30 39 30 393
Cuts at: 2591 2621 1178
Size: 30 4321
Fragments arranged by size:
4321 696 393 165 39 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2156 2405 2951 1186
Size: 970 249 546 3999
Fragments arranged by size:
3999 970 546 249
BseYI C'CCAG_C
Cuts at: 4967 5463 4967
Size: 496 5268
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5191
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3214 3975 5003 1540
Size: 275 186 1213 761 1028 2301
Fragments arranged by size:
2301 1213 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2403 2484 2796 3825 3910 4981 2403
Size: 81 312 1029 85 1071 3186
Fragments arranged by size:
3186 1071 1029 312 85 81
BsmI GAATG_Cn'
Cuts at: 3275 3368 3275
Size: 93 5671
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2246 2284 2403 2484 2655 2796 3083
Size: 399 456 38 119 81 171 141 287
Cuts at: 3083 3825 3910 4981 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 456 399 287 171 141
119 85 81 38
BspEI T'CCGG_A
Cuts at: 5427 5427
Size: 5764
BspHI T'CATG_A
Cuts at: 3653 5383 3653
Size: 1730 4034
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3119 844
Size: 2275 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3651 1394
Size: 429 1828 3507
Fragments arranged by size:
3507 1828 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4259 4433 65
Size: 1183 3011 174 1396
Fragments arranged by size:
3011 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2199 2246 2565 2846 4408 4595 1700
Size: 110 389 47 319 281 1562 187 2869
Fragments arranged by size:
2869 1562 389 319 281 187 110 47
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5764
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5764
BssSI C'ACGA_G
Cuts at: 2138 3824 4836 2138
Size: 1686 1012 3066
Fragments arranged by size:
3066 1686 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2256 794
Size: 1462 4302
BstBI TT'CG_AA
Cuts at: 4568 4568
Size: 5764
BstEII G'GTnAC_C
Cuts at: 1184 2365 2686 1184
Size: 1181 321 4262
Fragments arranged by size:
4262 1181 321
BstXI CCAn_nnnn'nTGG
Cuts at: 2683 3007 2683
Size: 324 5440
BstYI r'GATC_y
Cuts at: 1972 2586 2940 3094 3461 3846 3863 5315 5401
Size: 614 354 154 367 385 17 1452 86
Cuts at: 5401 5413 5759 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 614 385 367 354 346 154
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4273
Fragments arranged by size:
4273 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5025
Fragments arranged by size:
5025 683 56
BtsI GCAGTG_nn'
Cuts at: 2071 3280 4074 4094 2071
Size: 1209 794 20 3741
Fragments arranged by size:
3741 1209 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5764
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5729
DraI TTT'AAA
Cuts at: 3068 3068
Size: 5764
DraIII CAC_nnn'GTG
Cuts at: 2365 2365
Size: 5764
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4771 809
Size: 507 3455 1802
Fragments arranged by size:
3455 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2050 2553 2640
Size: 54 1425 50 275 18 220 503 87
Cuts at: 2640 3211 4100 5729 8
Size: 571 889 1629 43
Fragments arranged by size:
1629 1425 889 571 503 275 220 87
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3211 1537
Size: 275 1399 4090
Fragments arranged by size:
4090 1399 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2213 2459 3694 1353
Size: 860 246 1235 3423
Fragments arranged by size:
3423 1235 860 246
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2072 4351 4725 4871 387
Size: 1064 138 483 2279 374 146 1280
Fragments arranged by size:
2279 1280 1064 483 374 146 138
EcoICRI GAG'CTC
Cuts at: 2401 2401
Size: 5764
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2171 3827 4424 5211 5479 1239
Size: 393 539 1656 597 787 268 1524
Fragments arranged by size:
1656 1524 787 597 539 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2210 2705 2210
Size: 495 5269
EcoO109I rG'GnC_Cy
Cuts at: 2280 2986 2280
Size: 706 5058
EcoRI G'AATT_C
Cuts at: 3073 3451 3073
Size: 378 5386
FspI TGC'GCA
Cuts at: 2165 4270 2165
Size: 2105 3659
FspAI rTGC'GCAy
Cuts at: 2165 2165
Size: 5764
HaeII r_GCGC'y
Cuts at: 1678 2510 4911 1678
Size: 832 2401 2531
Fragments arranged by size:
2531 2401 832
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2078 2110 2222 2254 4405
Size: 32 135 32 506 32 112 32 2151
Cuts at: 4405 4437 4479 4511 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2151 506 135 112 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3108 3354 669
Size: 988 281 1170 246 3079
Fragments arranged by size:
3079 1170 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5764
HpaI GTT'AAC
Cuts at: 3354 3354
Size: 5764
KasI G'GCGC_C
Cuts at: 2506 2506
Size: 5764
KpnI G_GTAC'C
Cuts at: 3089 3089
Size: 5764
MfeI C'AATT_G
Cuts at: 3363 3363
Size: 5764
MluI A'CGCG_T
Cuts at: 5640 5640
Size: 5764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4878 5062 1305
Size: 3573 184 2007
Fragments arranged by size:
3573 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2052 2642 10
Size: 54 1425 343 220 590 3132
Fragments arranged by size:
3132 1425 590 343 220 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2039 2957 3722 4081 4240 518
Size: 648 873 918 765 359 159 2042
Fragments arranged by size:
2042 918 873 765 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2683 3025 3180 3857 5005 5250 1606
Size: 1077 342 155 677 1148 245 2120
Fragments arranged by size:
2120 1148 1077 677 342 245 155
NarI GG'CG_CC
Cuts at: 2507 2507
Size: 5764
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4273
Fragments arranged by size:
4273 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5764
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5764
NotI GC'GGCC_GC
Cuts at: 3211 3211
Size: 5764
NspI r_CATG'y
Cuts at: 1132 1229 2263 2623 2962 3122 4667 1132
Size: 97 1034 360 339 160 1545 2229
Fragments arranged by size:
2229 1545 1034 360 339 160 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5764
PciI A'CATG_T
Cuts at: 2619 4663 2619
Size: 2044 3720
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5764
PmeI GTTT'AAAC
Cuts at: 3068 3068
Size: 5764
PmlI CAC'GTG
Cuts at: 2362 2362
Size: 5764
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3833 3865 5346 5378 3833
Size: 32 1481 32 4219
Fragments arranged by size:
4219 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2986 2986
Size: 5764
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5764
PsiI TTA'TAA
Cuts at: 3334 3334
Size: 5764
PspOMI G'GGCC_C
Cuts at: 2280 2280
Size: 5764
PstI C_TGCA'G
Cuts at: 830 3116 830
Size: 2286 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5764
PvuII CAG'CTG
Cuts at: 2683 3025 2683
Size: 342 5422
SacI G_AGCT'C
Cuts at: 2403 2403
Size: 5764
SalI G'TCGA_C
Cuts at: 1936 3106 1936
Size: 1170 4594
SbfI CC_TGCA'GG
Cuts at: 3116 3116
Size: 5764
ScaI AGT'ACT
Cuts at: 4012 5724 4012
Size: 1712 4052
SfcI C'TryA_G
Cuts at: 826 1045 3112 4247 4928 5119 826
Size: 219 2067 1135 681 191 1471
Fragments arranged by size:
2067 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2508 2508
Size: 5764
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5764
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5764
SmaI CCC'GGG
Cuts at: 1872 2492 3091 1872
Size: 620 599 4545
Fragments arranged by size:
4545 620 599
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3868 4769 5031 820
Size: 197 926 1925 901 262 1553
Fragments arranged by size:
1925 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3555 493
Size: 3062 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5764
SphI G_CATG'C
Cuts at: 1132 2962 3122 1132
Size: 1830 160 3774
Fragments arranged by size:
3774 1830 160
SspI AAT'ATT
Cuts at: 5 52 3688 5
Size: 47 3636 2081
Fragments arranged by size:
3636 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5764
StyI C'CwwG_G
Cuts at: 513 1065 2004 2271 3203 513
Size: 552 939 267 932 3074
Fragments arranged by size:
3074 939 932 552 267
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3791 3808 3961 4146 4591 325
Size: 1255 2211 17 153 185 445 1498
Fragments arranged by size:
2211 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2571 4010 5722
Size: 274 80 33 51 1443 594 1439 1712
Cuts at: 5722 96
Size: 138
Fragments arranged by size:
1712 1443 1439 594 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5724 5756 5724
Size: 32 5732
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5764
XbaI T'CTAG_A
Cuts at: 3100 3100
Size: 5764
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5764
XmaI C'CCGG_G
Cuts at: 1870 2490 3089 1870
Size: 620 599 4545
Fragments arranged by size:
4545 620 599
XmnI GAAnn'nnTTC
Cuts at: 3893 3893
Size: 5764
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2233 3572 276
Size: 53 83 186 936 699 1339 2468
Fragments arranged by size:
2468 1339 936 699 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I EcoRV FalI FseI NaeI NgoMIV
NruI NsiI PacI PfoI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SrfI SwaI XcmI