Restriction map for
pFN21AE1848
(Circular) (Six-base) MAPSORT of: pFN21AE1848.seq Check: 4740 from: 1 to: 5800
pFN21AE1848 5800 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2654 2786 2825 3098 2654
Size: 132 39 273 5356
Fragments arranged by size:
5356 273 132 39
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3610 278
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3084 3143 1937
Size: 1147 59 4594
Fragments arranged by size:
4594 1147 59
Acc65I G'GTAC_C
Cuts at: 3121 3121
Size: 5800
AclI AA'CG_TT
Cuts at: 1563 3927 4300 1563
Size: 2364 373 3063
Fragments arranged by size:
3063 2364 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2210 2753 3863 5247 5515 1632
Size: 578 543 1110 1384 268 1917
Fragments arranged by size:
1917 1384 1110 578 543 268
AfeI AGC'GCT
Cuts at: 2306 2447 2306
Size: 141 5659
AflII C'TTAA_G
Cuts at: 820 1017 2519 820
Size: 197 1502 4101
Fragments arranged by size:
4101 1502 197
AflIII A'CryG_T
Cuts at: 1203 4699 5676 1203
Size: 3496 977 1327
Fragments arranged by size:
3496 1327 977
AgeI A'CCGG_T
Cuts at: 4631 4631
Size: 5800
AhdI GACnn_n'nnGTC
Cuts at: 4529 4529
Size: 5800
AleI CACnn'nnGTG
Cuts at: 1166 2278 1166
Size: 1112 4688
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2296 2591 1184
Size: 1112 295 4393
Fragments arranged by size:
4393 1112 295
AlwNI CAG_nnn'CTG
Cuts at: 3543 5115 3543
Size: 1572 4228
ApaLI G'TGCA_C
Cuts at: 2271 2873 3064 3857 5013 2271
Size: 602 191 793 1156 3058
Fragments arranged by size:
3058 1156 793 602 191
ApoI r'AATT_y
Cuts at: 1493 2546 3109 3335 3487 5596 1493
Size: 1053 563 226 152 2109 1697
Fragments arranged by size:
2109 1697 1053 563 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4354 160
Size: 872 3322 1606
Fragments arranged by size:
3322 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
AvaI C'yCGr_G
Cuts at: 1870 1943 2230 2276 2827 2920 3125 1870
Size: 73 287 46 551 93 205 4545
Fragments arranged by size:
4545 551 287 205 93 73 46
BamHI G'GATC_C
Cuts at: 3130 3497 3130
Size: 367 5433
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2464 2704 2798 2924 2940
Size: 325 217 627 677 240 94 126 16
Cuts at: 2940 3121 4576 618
Size: 181 1455 1842
Fragments arranged by size:
1842 1455 677 627 325 240 217 181
126 94 16
BanII G_rGCy'C
Cuts at: 1391 2118 2584 3119 1391
Size: 727 466 535 4072
Fragments arranged by size:
4072 727 535 466
BbeI G_GCGC'C
Cuts at: 2708 2708
Size: 5800
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4829
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2518 2552 2780 2814 3991
Size: 34 50 34 497 34 228 34 1177
Cuts at: 3991 4025 1903
Size: 34 3678
Fragments arranged by size:
3678 1177 497 228 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3692 4902 3692
Size: 1210 4590
BclI T'GATC_A
Cuts at: 1548 2622 1548
Size: 1074 4726
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2162 2792 2831
Size: 107 122 71 631 605 490 630 39
Cuts at: 2831 2907 4411 136
Size: 76 1504 1525
Fragments arranged by size:
1525 1504 631 630 605 490 122 107
76 71 39
BglII A'GATC_T
Cuts at: 5795 5795
Size: 5800
BlpI GC'TnA_GC
Cuts at: 3217 3217
Size: 5800
Bme1580I G_kGCm'C
Cuts at: 1790 2275 2877 2927 3068 3861 5017 1790
Size: 485 602 50 141 793 1156 2573
Fragments arranged by size:
2573 1156 793 602 485 141 50
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5800
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3531 4489 442
Size: 416 53 83 399 24 2114 958 1753
Fragments arranged by size:
2114 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5800
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2162 2216 2342 2495 2847 4460 1239
Size: 923 54 126 153 352 1613 2579
Fragments arranged by size:
2579 1613 923 352 153 126 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2333 2741 3925 4790 5088 2333
Size: 408 1184 865 298 3045
Fragments arranged by size:
3045 1184 865 408 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2633 4463 882
Size: 617 1134 1830 2219
Fragments arranged by size:
2219 1830 1134 617
BsaAI yAC'GTr
Cuts at: 493 1204 3591 493
Size: 711 2387 2702
Fragments arranged by size:
2702 2387 711
BsaBI GATnn'nnATC
Cuts at: 5794 5794
Size: 5800
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2705 3607 3989 4624
Size: 53 83 186 936 1172 902 382 635
Cuts at: 4624 275
Size: 1451
Fragments arranged by size:
1451 1172 936 902 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4233 4631 4905 5052 5463 4233
Size: 398 274 147 411 4570
Fragments arranged by size:
4570 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5575
Fragments arranged by size:
5575 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2380 3092 1186
Size: 1194 712 3894
Fragments arranged by size:
3894 1194 712
BseYI C'CCAG_C
Cuts at: 2630 5003 5499 2630
Size: 2373 496 2931
Fragments arranged by size:
2931 2373 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2299 2446 3076 1163
Size: 573 563 147 630 3887
Fragments arranged by size:
3887 630 573 563 147
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2088 2157 2185 3250 4011 5039
Size: 275 186 87 69 28 1065 761 1028
Cuts at: 5039 1540
Size: 2301
Fragments arranged by size:
2301 1065 1028 761 275 186 87 69
28
BsiHKAI G_wGCw'C
Cuts at: 2275 2877 3068 3861 3946 5017 2275
Size: 602 191 793 85 1071 3058
Fragments arranged by size:
3058 1071 793 602 191 85
BsmI GAATG_Cn'
Cuts at: 3311 3404 3311
Size: 93 5707
BsmBI CGTCTCn'nnnn_
Cuts at: 2237 2741 2237
Size: 504 5296
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2118 2275 2584 2877 2927 3068 3119
Size: 399 328 157 309 293 50 141 51
Cuts at: 3119 3861 3946 5017 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 328 309 293 157
141 85 51 50
BspEI T'CCGG_A
Cuts at: 5463 5463
Size: 5800
BspHI T'CATG_A
Cuts at: 3689 5419 3689
Size: 1730 4070
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2462 2654 2696 2786 2825 3098 3155 844
Size: 1618 192 42 90 39 273 57 3489
Fragments arranged by size:
3489 1618 273 192 90 57 42 39
BsrBI CCG'CTC
Cuts at: 1394 1823 2200 3687 1394
Size: 429 377 1487 3507
Fragments arranged by size:
3507 1487 429 377
BsrDI GCAATG_nn'
Cuts at: 65 1248 4295 4469 65
Size: 1183 3047 174 1396
Fragments arranged by size:
3047 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2184 2786 4444 4631 1700
Size: 110 374 602 1658 187 2869
Fragments arranged by size:
2869 1658 602 374 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5800
BssHII G'CGCG_C
Cuts at: 1925 2027 1925
Size: 102 5698
BssSI C'ACGA_G
Cuts at: 2606 3860 4872 2606
Size: 1254 1012 3534
Fragments arranged by size:
3534 1254 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3063 794
Size: 2269 3531
BstBI TT'CG_AA
Cuts at: 4604 4604
Size: 5800
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5800
BstYI r'GATC_y
Cuts at: 1972 2572 3130 3497 3882 3899 5351 5437 5449
Size: 600 558 367 385 17 1452 86 12
Cuts at: 5449 5795 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 600 558 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2040 2656 2813 2980 513
Size: 552 975 616 157 167 3333
Fragments arranged by size:
3333 975 616 552 167 157
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2472 487
Size: 683 56 1246 3815
Fragments arranged by size:
3815 1246 683 56
BtsI GCAGTG_nn'
Cuts at: 2501 3068 3316 4110 4130 2501
Size: 567 248 794 20 4171
Fragments arranged by size:
4171 794 567 248 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5800
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5765
DraI TTT'AAA
Cuts at: 3104 3104
Size: 5800
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4807 809
Size: 507 3491 1802
Fragments arranged by size:
3491 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2037 2085 2154
Size: 54 1425 50 275 18 207 48 69
Cuts at: 2154 2415 2908 2932 2977 2983 3247 4136 5765
Size: 261 493 24 45 6 264 889 1629
Cuts at: 5765 8
Size: 43
Fragments arranged by size:
1629 1425 889 493 275 264 261 207
69 54 50 48 45 43 24 18
6
EagI C'GGCC_G
Cuts at: 1537 1812 2085 2154 3247 1537
Size: 275 273 69 1093 4090
Fragments arranged by size:
4090 1093 275 273 69
EarI CTCTTCn'nnn_
Cuts at: 1353 3730 1353
Size: 2377 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4387 4761 4907 387
Size: 1064 138 2798 374 146 1280
Fragments arranged by size:
2798 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2162 2210 2216 2342 2495 2753 2847
Size: 393 530 48 6 126 153 258 94
Cuts at: 2847 3863 4460 5247 5515 1239
Size: 1016 597 787 268 1524
Fragments arranged by size:
1524 1016 787 597 530 393 268 258
153 126 94 48 6
EcoO109I rG'GnC_Cy
Cuts at: 2016 2227 3015 2016
Size: 211 788 4801
Fragments arranged by size:
4801 788 211
EcoRI G'AATT_C
Cuts at: 2546 3109 3487 2546
Size: 563 378 4859
Fragments arranged by size:
4859 563 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2374 2374
Size: 5800
FspI TGC'GCA
Cuts at: 4306 4306
Size: 5800
HaeII r_GCGC'y
Cuts at: 1678 2308 2449 2708 4947 1678
Size: 630 141 259 2239 2531
Fragments arranged by size:
2531 2239 630 259 141
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4441 4473 4515 4547 1373
Size: 32 135 32 2869 32 42 32 2626
Fragments arranged by size:
2869 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3144 3390 669
Size: 988 281 1206 246 3079
Fragments arranged by size:
3079 1206 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5800
HpaI GTT'AAC
Cuts at: 3390 3390
Size: 5800
KasI G'GCGC_C
Cuts at: 2704 2704
Size: 5800
KpnI G_GTAC'C
Cuts at: 3125 3125
Size: 5800
MfeI C'AATT_G
Cuts at: 3399 3399
Size: 5800
MluI A'CGCG_T
Cuts at: 5676 5676
Size: 5800
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4914 5098 1305
Size: 3609 184 2007
Fragments arranged by size:
3609 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2417 10
Size: 54 1425 343 585 3393
Fragments arranged by size:
3393 1425 585 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2278 2742 2880 3758 4117 4276 518
Size: 648 1112 464 138 878 359 159 2042
Fragments arranged by size:
2042 1112 878 648 464 359 159 138
MspA1I CmG'CkG
Cuts at: 1606 2023 2042 2134 2209 2851 2930 2982 3216
Size: 417 19 92 75 642 79 52 234
Cuts at: 3216 3893 5041 5286 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 642 417 245 234 92
79 75 52 19
NaeI GCC'GGC
Cuts at: 2788 2788
Size: 5800
NarI GG'CG_CC
Cuts at: 2705 2705
Size: 5800
NcoI C'CATG_G
Cuts at: 513 1065 2656 513
Size: 552 1591 3657
Fragments arranged by size:
3657 1591 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5800
NgoMIV G'CCGG_C
Cuts at: 2786 2786
Size: 5800
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5800
NotI GC'GGCC_GC
Cuts at: 2154 3247 2154
Size: 1093 4707
NspI r_CATG'y
Cuts at: 1132 1229 2218 3158 4703 1132
Size: 97 989 940 1545 2229
Fragments arranged by size:
2229 1545 989 940 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5800
PciI A'CATG_T
Cuts at: 4699 4699
Size: 5800
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2719 1108
Size: 1611 4189
PmeI GTTT'AAAC
Cuts at: 3104 3104
Size: 5800
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2270 2302 3869 3901 5382 5414 2270
Size: 32 1567 32 1481 32 2656
Fragments arranged by size:
2656 1567 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2016 2227 2016
Size: 211 5589
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5800
PsiI TTA'TAA
Cuts at: 3370 3370
Size: 5800
PspXI vC'TCGA_Gb
Cuts at: 2276 2276
Size: 5800
PstI C_TGCA'G
Cuts at: 830 3152 830
Size: 2322 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5800
SacII CC_GC'GG
Cuts at: 2043 2983 2043
Size: 940 4860
SalI G'TCGA_C
Cuts at: 1936 3142 1936
Size: 1206 4594
SanDI GG'GwC_CC
Cuts at: 2016 2227 2016
Size: 211 5589
SbfI CC_TGCA'GG
Cuts at: 3152 3152
Size: 5800
ScaI AGT'ACT
Cuts at: 4048 5760 4048
Size: 1712 4088
SfcI C'TryA_G
Cuts at: 826 1045 3148 4283 4964 5155 826
Size: 219 2103 1135 681 191 1471
Fragments arranged by size:
2103 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2706 2706
Size: 5800
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5800
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5800
SmaI CCC'GGG
Cuts at: 1872 2232 2922 3127 1872
Size: 360 690 205 4545
Fragments arranged by size:
4545 690 360 205
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2276 2348 2519 2756 3904 4805
Size: 197 926 333 72 171 237 1148 901
Cuts at: 4805 5067 820
Size: 262 1553
Fragments arranged by size:
1553 1148 926 901 333 262 237 197
171 72
SnaBI TAC'GTA
Cuts at: 493 3591 493
Size: 3098 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5800
SphI G_CATG'C
Cuts at: 1132 3158 1132
Size: 2026 3774
SspI AAT'ATT
Cuts at: 5 52 3724 5
Size: 47 3672 2081
Fragments arranged by size:
3672 2081 47
StuI AGG'CCT
Cuts at: 1517 2177 2489 1517
Size: 660 312 4828
Fragments arranged by size:
4828 660 312
StyI C'CwwG_G
Cuts at: 513 1065 2418 2656 3239 513
Size: 552 1353 238 583 3074
Fragments arranged by size:
3074 1353 583 552 238
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2600 3827 3844 3997 4182 4627 325
Size: 1255 1020 1227 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1227 1020 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2523 4046 5758
Size: 274 80 33 51 1443 546 1523 1712
Cuts at: 5758 96
Size: 138
Fragments arranged by size:
1712 1523 1443 546 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5760 5792 5760
Size: 32 5768
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5800
XbaI T'CTAG_A
Cuts at: 3136 3136
Size: 5800
XhoI C'TCGA_G
Cuts at: 1943 2276 1943
Size: 333 5467
XmaI C'CCGG_G
Cuts at: 1870 2230 2920 3125 1870
Size: 360 690 205 4545
Fragments arranged by size:
4545 690 360 205
XmnI GAAnn'nnTTC
Cuts at: 3929 3929
Size: 5800
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3608 276
Size: 53 83 186 936 2074 2468
Fragments arranged by size:
2468 2074 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspXI PstI PvuI SacII
SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI
PspOMI PsrI PvuII RsrII SacI SapI SexAI SfiI
SrfI SwaI XcmI