Restriction map for
pFN21AE1845
(Circular) (Six-base) MAPSORT of: pFN21AE1845.seq Check: 9705 from: 1 to: 5788
pFN21AE1845 5788 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3598 278
Size: 53 83 186 936 2062 2468
Fragments arranged by size:
2468 2062 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3131 1937
Size: 1194 4594
Acc65I G'GTAC_C
Cuts at: 3109 3109
Size: 5788
AclI AA'CG_TT
Cuts at: 1563 3915 4288 1563
Size: 2352 373 3063
Fragments arranged by size:
3063 2352 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2429 2742 3851 5235 5503 1632
Size: 797 313 1109 1384 268 1917
Fragments arranged by size:
1917 1384 1109 797 313 268
AfeI AGC'GCT
Cuts at: 2070 2070
Size: 5788
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5591
AflIII A'CryG_T
Cuts at: 1203 4687 5664 1203
Size: 3484 977 1327
Fragments arranged by size:
3484 1327 977
AgeI A'CCGG_T
Cuts at: 4619 4619
Size: 5788
AhdI GACnn_n'nnGTC
Cuts at: 4517 4517
Size: 5788
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5788
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5788
AlwNI CAG_nnn'CTG
Cuts at: 2166 3010 3531 5103 2166
Size: 844 521 1572 2851
Fragments arranged by size:
2851 1572 844 521
ApaLI G'TGCA_C
Cuts at: 3845 5001 3845
Size: 1156 4632
ApoI r'AATT_y
Cuts at: 1493 2574 3097 3323 3475 5584 1493
Size: 1081 523 226 152 2109 1697
Fragments arranged by size:
2109 1697 1081 523 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4342 160
Size: 872 3310 1606
Fragments arranged by size:
3310 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
AvaI C'yCGr_G
Cuts at: 1870 1943 2025 2290 2834 3113 1870
Size: 73 82 265 544 279 4545
Fragments arranged by size:
4545 544 279 265 82 73
BamHI G'GATC_C
Cuts at: 3118 3485 3118
Size: 367 5421
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2839 3109 4564 618
Size: 325 217 627 1052 270 1455 1842
Fragments arranged by size:
1842 1455 1052 627 325 270 217
BanII G_rGCy'C
Cuts at: 1391 2297 2559 3107 1391
Size: 906 262 548 4072
Fragments arranged by size:
4072 906 548 262
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4817
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3979 4013 1903
Size: 34 2042 34 3678
Fragments arranged by size:
3678 2042 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3680 4890 3680
Size: 1210 4578
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5788
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2966 4399 136
Size: 107 122 71 631 605 1294 1433 1525
Fragments arranged by size:
1525 1433 1294 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5783 5783
Size: 5788
BlpI GC'TnA_GC
Cuts at: 3205 3205
Size: 5788
Bme1580I G_kGCm'C
Cuts at: 1790 3849 5005 1790
Size: 2059 1156 2573
Fragments arranged by size:
2573 2059 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5788
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3519 4477 442
Size: 416 53 83 399 24 2102 958 1753
Fragments arranged by size:
2102 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2906 3032 4448 1239
Size: 1667 126 1416 2579
Fragments arranged by size:
2579 1667 1416 126
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2609 3047 3913 4778 5076 2609
Size: 438 866 865 298 3321
Fragments arranged by size:
3321 866 865 438 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4451 882
Size: 617 2952 2219
Fragments arranged by size:
2952 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3579 493
Size: 711 2375 2702
Fragments arranged by size:
2702 2375 711
BsaBI GATnn'nnATC
Cuts at: 5782 5782
Size: 5788
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3595 3977 4612 275
Size: 53 83 186 936 2062 382 635 1451
Fragments arranged by size:
2062 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4221 4619 4893 5040 5451 4221
Size: 398 274 147 411 4558
Fragments arranged by size:
4558 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5563
Fragments arranged by size:
5563 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2281 1186
Size: 1095 4693
BseYI C'CCAG_C
Cuts at: 4991 5487 4991
Size: 496 5292
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2985 1163
Size: 573 1249 3966
Fragments arranged by size:
3966 1249 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2064 3238 3999 5027 1540
Size: 275 186 63 1174 761 1028 2301
Fragments arranged by size:
2301 1174 1028 761 275 186 63
BsiHKAI G_wGCw'C
Cuts at: 2361 2559 3849 3934 5005 2361
Size: 198 1290 85 1071 3144
Fragments arranged by size:
3144 1290 1071 198 85
BsmI GAATG_Cn'
Cuts at: 3299 3392 3299
Size: 93 5695
BsmBI CGTCTCn'nnnn_
Cuts at: 2293 2293
Size: 5788
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2297 2361 2559 3107 3849 3934 5005
Size: 399 507 64 198 548 742 85 1071
Cuts at: 5005 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 548 507 399 198 85
64
BspEI T'CCGG_A
Cuts at: 5451 5451
Size: 5788
BspHI T'CATG_A
Cuts at: 3677 5407 3677
Size: 1730 4058
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3143 844
Size: 2299 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2211 3675 1394
Size: 429 388 1464 3507
Fragments arranged by size:
3507 1464 429 388
BsrDI GCAATG_nn'
Cuts at: 65 1248 4283 4457 65
Size: 1183 3035 174 1396
Fragments arranged by size:
3035 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2198 4432 4619 1700
Size: 110 388 2234 187 2869
Fragments arranged by size:
2869 2234 388 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5788
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5788
BssSI C'ACGA_G
Cuts at: 3848 4860 3848
Size: 1012 4776
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5788
BstBI TT'CG_AA
Cuts at: 4592 4592
Size: 5788
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5788
BstYI r'GATC_y
Cuts at: 1972 2651 3118 3485 3870 3887 5339 5425 5437
Size: 679 467 367 385 17 1452 86 12
Cuts at: 5437 5783 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 679 467 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5236
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5049
Fragments arranged by size:
5049 683 56
BtsI GCAGTG_nn'
Cuts at: 2119 2456 3304 4098 4118 2119
Size: 337 848 794 20 3789
Fragments arranged by size:
3789 848 794 337 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5788
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2167 2202 529
Size: 35 1603 35 4115
Fragments arranged by size:
4115 1603 35 35
DraI TTT'AAA
Cuts at: 3092 3092
Size: 5788
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4795 809
Size: 507 3479 1802
Fragments arranged by size:
3479 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2061 2100 3235
Size: 54 1425 50 275 18 231 39 1135
Cuts at: 3235 4124 5753 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1135 889 275 231 54 50
43 39 18
EagI C'GGCC_G
Cuts at: 1537 1812 2061 3235 1537
Size: 275 249 1174 4090
Fragments arranged by size:
4090 1174 275 249
EarI CTCTTCn'nnn_
Cuts at: 1353 2284 2616 3718 1353
Size: 931 332 1102 3423
Fragments arranged by size:
3423 1102 931 332
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4375 4749 4895 387
Size: 1064 138 2786 374 146 1280
Fragments arranged by size:
2786 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2557 2557
Size: 5788
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2429 2742 2906 3032 3851 4448 5235
Size: 393 797 313 164 126 819 597 787
Cuts at: 5235 5503 1239
Size: 268 1524
Fragments arranged by size:
1524 819 797 787 597 393 313 268
164 126
EcoRI G'AATT_C
Cuts at: 3097 3475 3097
Size: 378 5410
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2459 2459
Size: 5788
FspI TGC'GCA
Cuts at: 4294 4294
Size: 5788
HaeII r_GCGC'y
Cuts at: 1678 2072 2142 2257 4935 1678
Size: 394 70 115 2678 2531
Fragments arranged by size:
2678 2531 394 115 70
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2546 2578 4429 4461 4503
Size: 32 135 32 974 32 1851 32 42
Cuts at: 4503 4535 1373
Size: 32 2626
Fragments arranged by size:
2626 1851 974 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3132 3378 669
Size: 988 281 1194 246 3079
Fragments arranged by size:
3079 1194 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5788
HpaI GTT'AAC
Cuts at: 3378 3378
Size: 5788
KpnI G_GTAC'C
Cuts at: 3113 3113
Size: 5788
MfeI C'AATT_G
Cuts at: 3387 3387
Size: 5788
MluI A'CGCG_T
Cuts at: 5664 5664
Size: 5788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4902 5086 1305
Size: 3597 184 2007
Fragments arranged by size:
3597 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2102 10
Size: 54 1425 343 270 3696
Fragments arranged by size:
3696 1425 343 270 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3746 4105 4264 518
Size: 648 2580 359 159 2042
Fragments arranged by size:
2580 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2106 2163 2220 2828 3204 3881 5029 5274
Size: 500 57 57 608 376 677 1148 245
Cuts at: 5274 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 608 500 376 245 57
57
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5236
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5788
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5788
NotI GC'GGCC_GC
Cuts at: 2061 3235 2061
Size: 1174 4614
NspI r_CATG'y
Cuts at: 1132 1229 3146 4691 1132
Size: 97 1917 1545 2229
Fragments arranged by size:
2229 1917 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5788
PciI A'CATG_T
Cuts at: 4687 4687
Size: 5788
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5788
PmeI GTTT'AAAC
Cuts at: 3092 3092
Size: 5788
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3857 3889 5370 5402 3857
Size: 32 1481 32 4243
Fragments arranged by size:
4243 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5788
PsiI TTA'TAA
Cuts at: 3358 3358
Size: 5788
PstI C_TGCA'G
Cuts at: 830 3140 830
Size: 2310 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5788
PvuII CAG'CTG
Cuts at: 2163 2220 2828 2163
Size: 57 608 5123
Fragments arranged by size:
5123 608 57
SacI G_AGCT'C
Cuts at: 2559 2559
Size: 5788
SalI G'TCGA_C
Cuts at: 1936 3130 1936
Size: 1194 4594
SbfI CC_TGCA'GG
Cuts at: 3140 3140
Size: 5788
ScaI AGT'ACT
Cuts at: 4036 5748 4036
Size: 1712 4076
SfcI C'TryA_G
Cuts at: 826 1045 3136 4271 4952 5143 826
Size: 219 2091 1135 681 191 1471
Fragments arranged by size:
2091 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
SgrAI Cr'CCGG_yG
Cuts at: 1700 2198 1700
Size: 498 5290
SmaI CCC'GGG
Cuts at: 1872 2027 3115 1872
Size: 155 1088 4545
Fragments arranged by size:
4545 1088 155
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2588 3026 3892 4793 5055 820
Size: 197 926 645 438 866 901 262 1553
Fragments arranged by size:
1553 926 901 866 645 438 262 197
SnaBI TAC'GTA
Cuts at: 493 3579 493
Size: 3086 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5788
SphI G_CATG'C
Cuts at: 1132 3146 1132
Size: 2014 3774
SspI AAT'ATT
Cuts at: 5 52 3712 5
Size: 47 3660 2081
Fragments arranged by size:
3660 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5788
StyI C'CwwG_G
Cuts at: 513 1065 3227 513
Size: 552 2162 3074
Fragments arranged by size:
3074 2162 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3815 3832 3985 4170 4615 325
Size: 1255 2235 17 153 185 445 1498
Fragments arranged by size:
2235 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2764 4034 5746
Size: 274 80 33 51 1443 787 1270 1712
Cuts at: 5746 96
Size: 138
Fragments arranged by size:
1712 1443 1270 787 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5748 5780 5748
Size: 32 5756
Tth111I GACn'n_nGTC
Cuts at: 1316 2395 1316
Size: 1079 4709
XbaI T'CTAG_A
Cuts at: 3124 3124
Size: 5788
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2864 2864
Size: 5788
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5788
XmaI C'CCGG_G
Cuts at: 1870 2025 3113 1870
Size: 155 1088 4545
Fragments arranged by size:
4545 1088 155
XmnI GAAnn'nnTTC
Cuts at: 2442 2988 3917 2442
Size: 546 929 4313
Fragments arranged by size:
4313 929 546
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3596 276
Size: 53 83 186 936 2062 2468
Fragments arranged by size:
2468 2062 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI