Restriction map for
pFN21AE1843
(Circular) (Six-base) MAPSORT of: pFN21AE1843.seq Check: 4173 from: 1 to: 5779
pFN21AE1843 5779 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2514 2514
Size: 5779
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3589 278
Size: 53 83 186 936 2053 2468
Fragments arranged by size:
2468 2053 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3122 1937
Size: 1185 4594
Acc65I G'GTAC_C
Cuts at: 3100 3100
Size: 5779
AclI AA'CG_TT
Cuts at: 1563 3906 4279 1563
Size: 2343 373 3063
Fragments arranged by size:
3063 2343 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2076 3842 5226 5494 1632
Size: 444 1766 1384 268 1917
Fragments arranged by size:
1917 1766 1384 444 268
AfeI AGC'GCT
Cuts at: 2098 2098
Size: 5779
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5582
AflIII A'CryG_T
Cuts at: 1203 2988 4678 5655 1203
Size: 1785 1690 977 1327
Fragments arranged by size:
1785 1690 1327 977
AgeI A'CCGG_T
Cuts at: 4610 4610
Size: 5779
AhdI GACnn_n'nnGTC
Cuts at: 4508 4508
Size: 5779
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5779
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5779
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2928 2960 2928
Size: 32 5747
AlwNI CAG_nnn'CTG
Cuts at: 2604 3522 5094 2604
Size: 918 1572 3289
Fragments arranged by size:
3289 1572 918
ApaLI G'TGCA_C
Cuts at: 3836 4992 3836
Size: 1156 4623
ApoI r'AATT_y
Cuts at: 1493 3088 3314 3466 5575 1493
Size: 1595 226 152 2109 1697
Fragments arranged by size:
2109 1697 1595 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4333 160
Size: 872 3301 1606
Fragments arranged by size:
3301 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5779
AvaI C'yCGr_G
Cuts at: 1870 1943 3104 1870
Size: 73 1161 4545
Fragments arranged by size:
4545 1161 73
BamHI G'GATC_C
Cuts at: 3109 3476 3109
Size: 367 5412
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3100 4555 618
Size: 325 217 627 1313 1455 1842
Fragments arranged by size:
1842 1455 1313 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2311 2645 3098 1391
Size: 920 334 453 4072
Fragments arranged by size:
4072 920 453 334
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2298 928
Size: 971 399 4409
Fragments arranged by size:
4409 971 399
BbvCI CC'TCA_GC
Cuts at: 2093 2093
Size: 5779
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3970 4004 1903
Size: 34 50 34 1949 34 3678
Fragments arranged by size:
3678 1949 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2333 3671 4881 2333
Size: 1338 1210 3231
Fragments arranged by size:
3231 1338 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5779
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4390 136
Size: 107 122 71 631 605 337 2381 1525
Fragments arranged by size:
2381 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5774 5774
Size: 5779
BlpI GC'TnA_GC
Cuts at: 3196 3196
Size: 5779
Bme1580I G_kGCm'C
Cuts at: 1790 3840 4996 1790
Size: 2050 1156 2573
Fragments arranged by size:
2573 2050 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5779
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2614 2734 3510
Size: 416 53 83 399 24 1197 120 776
Cuts at: 3510 4468 442
Size: 958 1753
Fragments arranged by size:
1753 1197 958 776 416 399 120 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5779
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2883 2883
Size: 5779
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4439 1239
Size: 3200 2579
Bpu10I CC'TnA_GC
Cuts at: 2093 2093
Size: 5779
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2295 3904 4769 5067 2295
Size: 1609 865 298 3007
Fragments arranged by size:
3007 1609 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2112 2423 4442 882
Size: 617 613 311 2019 2219
Fragments arranged by size:
2219 2019 617 613 311
BsaAI yAC'GTr
Cuts at: 493 1204 3570 493
Size: 711 2366 2702
Fragments arranged by size:
2702 2366 711
BsaBI GATnn'nnATC
Cuts at: 2214 5773 2214
Size: 3559 2220
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3586 3968 4603 275
Size: 53 83 186 936 2053 382 635 1451
Fragments arranged by size:
2053 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4212 4610 4884 5031 5442 4212
Size: 398 274 147 411 4549
Fragments arranged by size:
4549 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5554
Fragments arranged by size:
5554 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2042 2687 2807 1186
Size: 856 645 120 4158
Fragments arranged by size:
4158 856 645 120
BseYI C'CCAG_C
Cuts at: 4982 5478 4982
Size: 496 5283
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2764 1163
Size: 573 1028 4178
Fragments arranged by size:
4178 1028 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3229 3990 5018 1540
Size: 275 186 1228 761 1028 2301
Fragments arranged by size:
2301 1228 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2311 2645 3840 3925 4996 2311
Size: 334 1195 85 1071 3094
Fragments arranged by size:
3094 1195 1071 334 85
BsmI GAATG_Cn'
Cuts at: 3290 3383 3290
Size: 93 5686
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2311 2645 3098 3840 3925 4996 1391
Size: 399 521 334 453 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 521 453 399 334 85
BspEI T'CCGG_A
Cuts at: 5442 5442
Size: 5779
BspHI T'CATG_A
Cuts at: 3668 5398 3668
Size: 1730 4049
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2514 3134 844
Size: 1670 620 3489
Fragments arranged by size:
3489 1670 620
BsrBI CCG'CTC
Cuts at: 1394 1823 3666 1394
Size: 429 1843 3507
Fragments arranged by size:
3507 1843 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4274 4448 65
Size: 1183 3026 174 1396
Fragments arranged by size:
3026 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4423 4610 1700
Size: 110 2613 187 2869
Fragments arranged by size:
2869 2613 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5779
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5779
BssSI C'ACGA_G
Cuts at: 3839 4851 3839
Size: 1012 4767
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5779
BstBI TT'CG_AA
Cuts at: 4583 4583
Size: 5779
BstEII G'GTnAC_C
Cuts at: 1184 2921 2954 1184
Size: 1737 33 4009
Fragments arranged by size:
4009 1737 33
BstYI r'GATC_y
Cuts at: 1972 2031 2635 3109 3476 3861 3878 5330 5416
Size: 59 604 474 367 385 17 1452 86
Cuts at: 5416 5428 5774 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 604 474 385 367 346 86
59 17 12
Bsu36I CC'TnA_GG
Cuts at: 2025 2025
Size: 5779
BtgI C'CryG_G
Cuts at: 513 1065 2917 513
Size: 552 1852 3375
Fragments arranged by size:
3375 1852 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2410 487
Size: 683 56 1184 3856
Fragments arranged by size:
3856 1184 683 56
BtsI GCAGTG_nn'
Cuts at: 3054 3295 4089 4109 3054
Size: 241 794 20 4724
Fragments arranged by size:
4724 794 241 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5779
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3043 3078 529
Size: 35 2479 35 3230
Fragments arranged by size:
3230 2479 35 35
DraI TTT'AAA
Cuts at: 3083 3083
Size: 5779
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4786 809
Size: 507 3470 1802
Fragments arranged by size:
3470 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3226 4115 5744
Size: 54 1425 50 275 18 1396 889 1629
Cuts at: 5744 8
Size: 43
Fragments arranged by size:
1629 1425 1396 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3226 1537
Size: 275 1414 4090
Fragments arranged by size:
4090 1414 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2052 2426 2438 2506 2658 2685 2829 2844
Size: 699 374 12 68 152 27 144 15
Cuts at: 2844 3709 1353
Size: 865 3423
Fragments arranged by size:
3423 865 699 374 152 144 68 27
15 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4366 4740 4886 387
Size: 1064 138 2777 374 146 1280
Fragments arranged by size:
2777 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2309 2643 2309
Size: 334 5445
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2076 3842 4439 5226 5494 1239
Size: 393 444 1766 597 787 268 1524
Fragments arranged by size:
1766 1524 787 597 444 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2024 2474 2024
Size: 450 5329
EcoO109I rG'GnC_Cy
Cuts at: 2463 2902 2463
Size: 439 5340
EcoRI G'AATT_C
Cuts at: 3088 3466 3088
Size: 378 5401
FspI TGC'GCA
Cuts at: 4285 4285
Size: 5779
HaeII r_GCGC'y
Cuts at: 1678 2100 4926 1678
Size: 422 2826 2531
Fragments arranged by size:
2826 2531 422
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2627 2659 2739 2771 2924
Size: 32 135 32 1055 32 80 32 153
Cuts at: 2924 2956 4420 4452 4494 4526 1373
Size: 32 1464 32 42 32 2626
Fragments arranged by size:
2626 1464 1055 153 135 80 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3123 3369 669
Size: 988 281 1185 246 3079
Fragments arranged by size:
3079 1185 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5779
HpaI GTT'AAC
Cuts at: 3369 3369
Size: 5779
KpnI G_GTAC'C
Cuts at: 3104 3104
Size: 5779
MfeI C'AATT_G
Cuts at: 3378 3378
Size: 5779
MluI A'CGCG_T
Cuts at: 5655 5655
Size: 5779
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2971 4893 5077 1305
Size: 1666 1922 184 2007
Fragments arranged by size:
2007 1922 1666 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3957
Fragments arranged by size:
3957 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3737 4096 4255 518
Size: 648 2571 359 159 2042
Fragments arranged by size:
2571 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2223 3195 3872 5020 5265 1606
Size: 617 972 677 1148 245 2120
Fragments arranged by size:
2120 1148 972 677 617 245
NcoI C'CATG_G
Cuts at: 513 1065 2917 513
Size: 552 1852 3375
Fragments arranged by size:
3375 1852 552
NdeI CA'TA_TG
Cuts at: 387 2659 2698 387
Size: 2272 39 3468
Fragments arranged by size:
3468 2272 39
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5779
NotI GC'GGCC_GC
Cuts at: 3226 3226
Size: 5779
NspI r_CATG'y
Cuts at: 1132 1229 2992 3137 4682 1132
Size: 97 1763 145 1545 2229
Fragments arranged by size:
2229 1763 1545 145 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5779
PciI A'CATG_T
Cuts at: 2988 4678 2988
Size: 1690 4089
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2604 2732 1108
Size: 1496 128 4155
Fragments arranged by size:
4155 1496 128
PfoI T'CCnGG_A
Cuts at: 2043 2043
Size: 5779
PmeI GTTT'AAAC
Cuts at: 3083 3083
Size: 5779
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3848 3880 5361 5393 3848
Size: 32 1481 32 4234
Fragments arranged by size:
4234 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2463 2463
Size: 5779
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5779
PsiI TTA'TAA
Cuts at: 3349 3349
Size: 5779
PstI C_TGCA'G
Cuts at: 830 3131 830
Size: 2301 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5779
SacI G_AGCT'C
Cuts at: 2311 2645 2311
Size: 334 5445
SalI G'TCGA_C
Cuts at: 1936 3121 1936
Size: 1185 4594
SapI GCTCTTCn'nnn_
Cuts at: 2438 2829 2438
Size: 391 5388
SbfI CC_TGCA'GG
Cuts at: 3131 3131
Size: 5779
ScaI AGT'ACT
Cuts at: 4027 5739 4027
Size: 1712 4067
SfcI C'TryA_G
Cuts at: 826 1045 2787 3127 4262 4943 5134 826
Size: 219 1742 340 1135 681 191 1471
Fragments arranged by size:
1742 1471 1135 681 340 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5779
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5779
SmaI CCC'GGG
Cuts at: 1872 3106 1872
Size: 1234 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2310 3883 4784 5046 820
Size: 197 926 367 1573 901 262 1553
Fragments arranged by size:
1573 1553 926 901 367 262 197
SnaBI TAC'GTA
Cuts at: 493 3570 493
Size: 3077 2702
SpeI A'CTAG_T
Cuts at: 152 2582 152
Size: 2430 3349
SphI G_CATG'C
Cuts at: 1132 3137 1132
Size: 2005 3774
SspI AAT'ATT
Cuts at: 5 52 3703 5
Size: 47 3651 2081
Fragments arranged by size:
3651 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5779
StyI C'CwwG_G
Cuts at: 513 1065 2179 2917 3218 513
Size: 552 1114 738 301 3074
Fragments arranged by size:
3074 1114 738 552 301
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2596 2992 3806 3823 3976 4161 4606
Size: 1255 1016 396 814 17 153 185 445
Cuts at: 4606 325
Size: 1498
Fragments arranged by size:
1498 1255 1016 814 445 396 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4025 5737 96
Size: 274 80 33 51 1443 2048 1712 138
Fragments arranged by size:
2048 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5739 5771 5739
Size: 32 5747
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5779
XbaI T'CTAG_A
Cuts at: 2845 3115 2845
Size: 270 5509
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2605 2605
Size: 5779
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5779
XmaI C'CCGG_G
Cuts at: 1870 3104 1870
Size: 1234 4545
XmnI GAAnn'nnTTC
Cuts at: 2061 2515 3908 2061
Size: 454 1393 3932
Fragments arranged by size:
3932 1393 454
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3587 276
Size: 53 83 186 936 2053 2468
Fragments arranged by size:
2468 2053 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI
BstXI BstZ17I DraIII EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI
PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI