Restriction map for
pFN21AE1842
(Circular) (Six-base) MAPSORT of: pFN21AE1842.seq Check: 6720 from: 1 to: 5779
pFN21AE1842 5779 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2067 2149 2628 2670 2067
Size: 82 479 42 5176
Fragments arranged by size:
5176 479 82 42
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2186 3589 278
Size: 53 83 186 936 650 1403 2468
Fragments arranged by size:
2468 1403 936 650 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3122 1937
Size: 1185 4594
Acc65I G'GTAC_C
Cuts at: 3100 3100
Size: 5779
AclI AA'CG_TT
Cuts at: 1563 3906 4279 1563
Size: 2343 373 3063
Fragments arranged by size:
3063 2343 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3842 5226 5494 1632
Size: 2210 1384 268 1917
Fragments arranged by size:
2210 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5582
AflIII A'CryG_T
Cuts at: 1203 4678 5655 1203
Size: 3475 977 1327
Fragments arranged by size:
3475 1327 977
AgeI A'CCGG_T
Cuts at: 4610 4610
Size: 5779
AhdI GACnn_n'nnGTC
Cuts at: 4508 4508
Size: 5779
AleI CACnn'nnGTG
Cuts at: 1166 2696 1166
Size: 1530 4249
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2075 1184
Size: 891 4888
AlwNI CAG_nnn'CTG
Cuts at: 3522 5094 3522
Size: 1572 4207
ApaI G_GGCC'C
Cuts at: 2120 2294 2348 2385 2750 2120
Size: 174 54 37 365 5149
Fragments arranged by size:
5149 365 174 54 37
ApaLI G'TGCA_C
Cuts at: 3836 4992 3836
Size: 1156 4623
ApoI r'AATT_y
Cuts at: 1493 3088 3314 3466 5575 1493
Size: 1595 226 152 2109 1697
Fragments arranged by size:
2109 1697 1595 226 152
AscI GG'CGCG_CC
Cuts at: 2334 2334
Size: 5779
AseI AT'TA_AT
Cuts at: 160 1032 4333 160
Size: 872 3301 1606
Fragments arranged by size:
3301 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5779
AvaI C'yCGr_G
Cuts at: 1870 1943 2106 2329 2366 3104 1870
Size: 73 163 223 37 738 4545
Fragments arranged by size:
4545 738 223 163 73 37
BamHI G'GATC_C
Cuts at: 3109 3476 3109
Size: 367 5412
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2354 2458 2729 2774 3100
Size: 325 217 627 567 104 271 45 326
Cuts at: 3100 4555 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 567 326 325 271 217
104 45
BanII G_rGCy'C
Cuts at: 1391 2069 2120 2294 2348 2385 2439 2496 2750
Size: 678 51 174 54 37 54 57 254
Cuts at: 2750 3050 3098 1391
Size: 300 48 4072
Fragments arranged by size:
4072 678 300 254 174 57 54 54
51 48 37
BbeI G_GCGC'C
Cuts at: 2358 2462 2778 2358
Size: 104 316 5359
Fragments arranged by size:
5359 316 104
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4808
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2866 2900 3970 4004 1903
Size: 34 929 34 1070 34 3678
Fragments arranged by size:
3678 1070 929 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3671 4881 3671
Size: 1210 4569
BclI T'GATC_A
Cuts at: 1548 2481 1548
Size: 933 4846
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2282 2298 2352
Size: 107 122 71 631 605 610 16 54
Cuts at: 2352 2745 4390 136
Size: 393 1645 1525
Fragments arranged by size:
1645 1525 631 610 605 393 122 107
71 54 16
BglII A'GATC_T
Cuts at: 5774 5774
Size: 5779
BlpI GC'TnA_GC
Cuts at: 2996 3196 2996
Size: 200 5579
Bme1580I G_kGCm'C
Cuts at: 1790 2120 2294 2348 2385 2703 2750 3840 4996
Size: 330 174 54 37 318 47 1090 1156
Cuts at: 4996 1790
Size: 2573
Fragments arranged by size:
2573 1156 1090 330 318 174 54 47
37
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5779
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3510 4468 442
Size: 416 53 83 399 24 2093 958 1753
Fragments arranged by size:
2093 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5779
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2453 2969 4439 1239
Size: 1214 516 1470 2579
Fragments arranged by size:
2579 1470 1214 516
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2747 3904 4769 5067 2747
Size: 1157 865 298 3459
Fragments arranged by size:
3459 1157 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4442 882
Size: 617 2943 2219
Fragments arranged by size:
2943 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3570 493
Size: 711 2366 2702
Fragments arranged by size:
2702 2366 711
BsaBI GATnn'nnATC
Cuts at: 5773 5773
Size: 5779
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2183 2355 2459 2775
Size: 53 83 186 936 650 172 104 316
Cuts at: 2775 3586 3968 4603 275
Size: 811 382 635 1451
Fragments arranged by size:
1451 936 811 650 635 382 316 186
172 104 83 53
BsaWI w'CCGG_w
Cuts at: 4212 4610 4884 5031 5442 4212
Size: 398 274 147 411 4549
Fragments arranged by size:
4549 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2426 2456 1178
Size: 30 165 30 1023 30 4501
Fragments arranged by size:
4501 1023 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2516 1186
Size: 1330 4449
BseYI C'CCAG_C
Cuts at: 2081 2158 2702 2770 4982 5478 2081
Size: 77 544 68 2212 496 2382
Fragments arranged by size:
2382 2212 544 496 77 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5206
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2016 2217 2286 2409 2474 3229
Size: 275 186 15 201 69 123 65 755
Cuts at: 3229 3990 5018 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 761 755 275 201 186 123
69 65 15
BsiHKAI G_wGCw'C
Cuts at: 2178 3050 3840 3925 4996 2178
Size: 872 790 85 1071 2961
Fragments arranged by size:
2961 1071 872 790 85
BsmI GAATG_Cn'
Cuts at: 3290 3383 3290
Size: 93 5686
BsmBI CGTCTCn'nnnn_
Cuts at: 2174 2924 2174
Size: 750 5029
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2069 2120 2178 2294 2348 2385 2439
Size: 399 279 51 58 116 54 37 54
Cuts at: 2439 2496 2703 2750 3050 3098 3840 3925 4996
Size: 57 207 47 300 48 742 85 1071
Cuts at: 4996 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 300 279 207 116
85 58 57 54 54 51 48 47
37
BspEI T'CCGG_A
Cuts at: 5442 5442
Size: 5779
BspHI T'CATG_A
Cuts at: 3668 5398 3668
Size: 1730 4049
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2067 2149 2628 2670 3134 844
Size: 1223 82 479 42 464 3489
Fragments arranged by size:
3489 1223 479 464 82 42
BsrBI CCG'CTC
Cuts at: 1394 1823 3666 1394
Size: 429 1843 3507
Fragments arranged by size:
3507 1843 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4274 4448 65
Size: 1183 3026 174 1396
Fragments arranged by size:
3026 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2259 2411 2461 2531 2735 4423 4610
Size: 110 449 152 50 70 204 1688 187
Cuts at: 4610 1700
Size: 2869
Fragments arranged by size:
2869 1688 449 204 187 152 110 70
50
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5779
BssHII G'CGCG_C
Cuts at: 1925 2334 2757 1925
Size: 409 423 4947
Fragments arranged by size:
4947 423 409
BssSI C'ACGA_G
Cuts at: 2177 3839 4851 2177
Size: 1662 1012 3105
Fragments arranged by size:
3105 1662 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2944 794
Size: 2150 3629
BstBI TT'CG_AA
Cuts at: 4583 4583
Size: 5779
BstEII G'GTnAC_C
Cuts at: 1184 2672 1184
Size: 1488 4291
BstXI CCAn_nnnn'nTGG
Cuts at: 2521 2521
Size: 5779
BstYI r'GATC_y
Cuts at: 1972 3109 3476 3861 3878 5330 5416 5428 5774
Size: 1137 367 385 17 1452 86 12 346
Cuts at: 5774 1972
Size: 1977
Fragments arranged by size:
1977 1452 1137 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2036 2286 2295 2340 3063 513
Size: 552 939 32 250 9 45 723 3229
Fragments arranged by size:
3229 939 723 552 250 45 32 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2869 487
Size: 683 56 1643 3397
Fragments arranged by size:
3397 1643 683 56
BtsI GCAGTG_nn'
Cuts at: 2638 3295 4089 4109 2638
Size: 657 794 20 4308
Fragments arranged by size:
4308 794 657 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5779
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5744
DraI TTT'AAA
Cuts at: 3083 3083
Size: 5779
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4786 809
Size: 507 3470 1802
Fragments arranged by size:
3470 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 2214 2238
Size: 54 1425 50 275 18 183 201 24
Cuts at: 2238 2283 2406 2471 2529 2607 2644 3226 4115
Size: 45 123 65 58 78 37 582 889
Cuts at: 4115 5744 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 582 275 201 183 123
78 65 58 54 50 45 43 37
24 18
EagI C'GGCC_G
Cuts at: 1537 1812 2013 2214 2283 2406 2471 3226 1537
Size: 275 201 201 69 123 65 755 4090
Fragments arranged by size:
4090 755 275 201 201 123 69 65
EarI CTCTTCn'nnn_
Cuts at: 1353 2543 3709 1353
Size: 1190 1166 3423
Fragments arranged by size:
3423 1190 1166
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2045 3020 4366 4740 4886 387
Size: 1064 138 456 975 1346 374 146 1280
Fragments arranged by size:
1346 1280 1064 975 456 374 146 138
EcoICRI GAG'CTC
Cuts at: 3048 3048
Size: 5779
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2453 2969 3842 4439 5226 5494 1239
Size: 393 821 516 873 597 787 268 1524
Fragments arranged by size:
1524 873 821 787 597 516 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2117 2291 2345 2382 2747 2117
Size: 174 54 37 365 5149
Fragments arranged by size:
5149 365 174 54 37
EcoRI G'AATT_C
Cuts at: 3088 3466 3088
Size: 378 5401
FseI GG_CCGG'CC
Cuts at: 2535 2535
Size: 5779
FspI TGC'GCA
Cuts at: 2894 2978 4285 2894
Size: 84 1307 4388
Fragments arranged by size:
4388 1307 84
HaeII r_GCGC'y
Cuts at: 1678 2091 2202 2358 2462 2468 2528 2778 4926
Size: 413 111 156 104 6 60 250 2148
Cuts at: 4926 1678
Size: 2531
Fragments arranged by size:
2531 2148 413 250 156 111 104 60
6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4420 4452 4494 4526 1373
Size: 32 135 32 2848 32 42 32 2626
Fragments arranged by size:
2848 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3123 3369 669
Size: 988 281 1185 246 3079
Fragments arranged by size:
3079 1185 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5779
HpaI GTT'AAC
Cuts at: 3369 3369
Size: 5779
KasI G'GCGC_C
Cuts at: 2354 2458 2774 2354
Size: 104 316 5359
Fragments arranged by size:
5359 316 104
KpnI G_GTAC'C
Cuts at: 3104 3104
Size: 5779
MfeI C'AATT_G
Cuts at: 3378 3378
Size: 5779
MluI A'CGCG_T
Cuts at: 5655 5655
Size: 5779
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4893 5077 1305
Size: 3588 184 2007
Fragments arranged by size:
3588 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3957
Fragments arranged by size:
3957 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2696 2888 3737 4096 4255 518
Size: 648 1530 192 849 359 159 2042
Fragments arranged by size:
2042 1530 849 648 359 192 159
MspA1I CmG'CkG
Cuts at: 1606 2038 2158 2342 2476 2521 2811 2995 3037
Size: 432 120 184 134 45 290 184 42
Cuts at: 3037 3195 3872 5020 5265 1606
Size: 158 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 432 290 245 184 184
158 134 120 45 42
NaeI GCC'GGC
Cuts at: 2261 2413 2463 2533 2737 2261
Size: 152 50 70 204 5303
Fragments arranged by size:
5303 204 152 70 50
NarI GG'CG_CC
Cuts at: 2355 2459 2775 2355
Size: 104 316 5359
Fragments arranged by size:
5359 316 104
NcoI C'CATG_G
Cuts at: 513 1065 2004 3063 513
Size: 552 939 1059 3229
Fragments arranged by size:
3229 1059 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5779
NgoMIV G'CCGG_C
Cuts at: 2259 2411 2461 2531 2735 2259
Size: 152 50 70 204 5303
Fragments arranged by size:
5303 204 152 70 50
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5779
NotI GC'GGCC_GC
Cuts at: 2406 2471 3226 2406
Size: 65 755 4959
Fragments arranged by size:
4959 755 65
NspI r_CATG'y
Cuts at: 1132 1229 3137 4682 1132
Size: 97 1908 1545 2229
Fragments arranged by size:
2229 1908 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5779
PciI A'CATG_T
Cuts at: 4678 4678
Size: 5779
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5779
PmeI GTTT'AAAC
Cuts at: 3083 3083
Size: 5779
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3848 3880 5361 5393 3848
Size: 32 1481 32 4234
Fragments arranged by size:
4234 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5779
PsiI TTA'TAA
Cuts at: 3349 3349
Size: 5779
PspOMI G'GGCC_C
Cuts at: 2116 2290 2344 2381 2746 2116
Size: 174 54 37 365 5149
Fragments arranged by size:
5149 365 174 54 37
PstI C_TGCA'G
Cuts at: 830 2064 2625 2667 3131 830
Size: 1234 561 42 464 3478
Fragments arranged by size:
3478 1234 561 464 42
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5779
PvuII CAG'CTG
Cuts at: 2521 2995 3037 2521
Size: 474 42 5263
Fragments arranged by size:
5263 474 42
SacI G_AGCT'C
Cuts at: 3050 3050
Size: 5779
SacII CC_GC'GG
Cuts at: 2039 2343 2039
Size: 304 5475
SalI G'TCGA_C
Cuts at: 1936 3121 1936
Size: 1185 4594
SbfI CC_TGCA'GG
Cuts at: 3131 3131
Size: 5779
ScaI AGT'ACT
Cuts at: 4027 5739 4027
Size: 1712 4067
SfcI C'TryA_G
Cuts at: 826 1045 2060 2621 2663 3127 4262 4943 5134
Size: 219 1015 561 42 464 1135 681 191
Cuts at: 5134 826
Size: 1471
Fragments arranged by size:
1471 1135 1015 681 561 464 219 191
42
SfiI GGCCn_nnn'nGGCC
Cuts at: 2282 2745 2282
Size: 463 5316
SfoI GGC'GCC
Cuts at: 2356 2460 2776 2356
Size: 104 316 5359
Fragments arranged by size:
5359 316 104
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5779
SgrAI Cr'CCGG_yG
Cuts at: 1700 2461 1700
Size: 761 5018
SmaI CCC'GGG
Cuts at: 1872 2108 2331 2368 3106 1872
Size: 236 223 37 738 4545
Fragments arranged by size:
4545 738 236 223 37
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2762 3883 4784 5046 820
Size: 197 926 819 1121 901 262 1553
Fragments arranged by size:
1553 1121 926 901 819 262 197
SnaBI TAC'GTA
Cuts at: 493 3570 493
Size: 3077 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5779
SphI G_CATG'C
Cuts at: 1132 3137 1132
Size: 2005 3774
SspI AAT'ATT
Cuts at: 5 52 3703 5
Size: 47 3651 2081
Fragments arranged by size:
3651 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5779
StyI C'CwwG_G
Cuts at: 513 1065 2004 2277 3051 3063 3218 513
Size: 552 939 273 774 12 155 3074
Fragments arranged by size:
3074 939 774 552 273 155 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3806 3823 3976 4161 4606 325
Size: 1255 2226 17 153 185 445 1498
Fragments arranged by size:
2226 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4025 5737 96
Size: 274 80 33 51 1443 2048 1712 138
Fragments arranged by size:
2048 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2168 2200 5739 5771 2168
Size: 32 3539 32 2176
Fragments arranged by size:
3539 2176 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5779
XbaI T'CTAG_A
Cuts at: 3115 3115
Size: 5779
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2284 2833 2284
Size: 549 5230
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5779
XmaI C'CCGG_G
Cuts at: 1870 2106 2329 2366 3104 1870
Size: 236 223 37 738 4545
Fragments arranged by size:
4545 738 236 223 37
XmnI GAAnn'nnTTC
Cuts at: 3908 3908
Size: 5779
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2184 3587 276
Size: 53 83 186 936 650 1403 2468
Fragments arranged by size:
2468 1403 936 650 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SacI SacII SalI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FspAI
NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI
RsrII SanDI SapI SexAI SrfI SwaI