Restriction map for
pFN21AE1840
(Circular) (Six-base) MAPSORT of: pFN21AE1840.seq Check: 1784 from: 1 to: 5776
pFN21AE1840 5776 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2681 3074 2681
Size: 393 5383
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3586 278
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3119 1937
Size: 1182 4594
Acc65I G'GTAC_C
Cuts at: 3097 3097
Size: 5776
AclI AA'CG_TT
Cuts at: 1563 3903 4276 1563
Size: 2340 373 3063
Fragments arranged by size:
3063 2340 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2180 2198 3839 5223 5491 1632
Size: 548 18 1641 1384 268 1917
Fragments arranged by size:
1917 1641 1384 548 268 18
AfeI AGC'GCT
Cuts at: 2294 2413 2429 2294
Size: 119 16 5641
Fragments arranged by size:
5641 119 16
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5579
AflIII A'CryG_T
Cuts at: 1203 2630 4675 5652 1203
Size: 1427 2045 977 1327
Fragments arranged by size:
2045 1427 1327 977
AgeI A'CCGG_T
Cuts at: 4607 4607
Size: 5776
AhdI GACnn_n'nnGTC
Cuts at: 4505 4505
Size: 5776
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5776
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5776
AlwNI CAG_nnn'CTG
Cuts at: 2716 2874 3519 5091 2716
Size: 158 645 1572 3401
Fragments arranged by size:
3401 1572 645 158
ApaI G_GGCC'C
Cuts at: 2155 2794 2155
Size: 639 5137
ApaLI G'TGCA_C
Cuts at: 3833 4989 3833
Size: 1156 4620
ApoI r'AATT_y
Cuts at: 1493 3085 3311 3463 5572 1493
Size: 1592 226 152 2109 1697
Fragments arranged by size:
2109 1697 1592 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4330 160
Size: 872 3298 1606
Fragments arranged by size:
3298 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
AvaI C'yCGr_G
Cuts at: 1870 1943 2400 2922 3101 1870
Size: 73 457 522 179 4545
Fragments arranged by size:
4545 522 457 179 73
BamHI G'GATC_C
Cuts at: 3106 3473 3106
Size: 367 5409
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3097 4552 618
Size: 325 217 627 1310 1455 1842
Fragments arranged by size:
1842 1455 1310 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2040 2155 2220 2794 3095 1391
Size: 649 115 65 574 301 4072
Fragments arranged by size:
4072 649 574 301 115 65
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4805
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3967 4001 1903
Size: 34 50 34 1946 34 3678
Fragments arranged by size:
3678 1946 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3668 4878 3668
Size: 1210 4566
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5776
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2313 4387 136
Size: 107 122 71 631 605 641 2074 1525
Fragments arranged by size:
2074 1525 641 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5771 5771
Size: 5776
BlpI GC'TnA_GC
Cuts at: 3193 3193
Size: 5776
Bme1580I G_kGCm'C
Cuts at: 1790 2126 2155 2569 2794 3837 4993 1790
Size: 336 29 414 225 1043 1156 2573
Fragments arranged by size:
2573 1156 1043 414 336 225 29
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5776
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3507 4465 442
Size: 416 53 83 399 24 2090 958 1753
Fragments arranged by size:
2090 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2330 2477 4436 1239
Size: 1091 147 1959 2579
Fragments arranged by size:
2579 1959 1091 147
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2285 2315 2486 3901 4766 5064 2285
Size: 30 171 1415 865 298 2997
Fragments arranged by size:
2997 1415 865 298 171 30
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2225 4439 882
Size: 617 726 2214 2219
Fragments arranged by size:
2219 2214 726 617
BsaAI yAC'GTr
Cuts at: 493 1204 2593 2631 3025 3567 493
Size: 711 1389 38 394 542 2702
Fragments arranged by size:
2702 1389 711 542 394 38
BsaBI GATnn'nnATC
Cuts at: 5770 5770
Size: 5776
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2105 2577 3583 3965
Size: 53 83 186 936 572 472 1006 382
Cuts at: 3965 4600 275
Size: 635 1451
Fragments arranged by size:
1451 1006 936 635 572 472 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4209 4607 4881 5028 5439 4209
Size: 398 274 147 411 4546
Fragments arranged by size:
4546 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3044 3074 1178
Size: 30 165 30 1641 30 3880
Fragments arranged by size:
3880 1641 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3065 1186
Size: 1879 3897
BseYI C'CCAG_C
Cuts at: 2414 2867 2882 4979 5475 2414
Size: 453 15 2097 496 2715
Fragments arranged by size:
2715 2097 496 453 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2416 3029 3065 1163
Size: 573 680 613 36 3874
Fragments arranged by size:
3874 680 613 573 36
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3226 3987 5015 1540
Size: 275 186 1225 761 1028 2301
Fragments arranged by size:
2301 1225 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2921 3837 3922 4993 2921
Size: 916 85 1071 3704
Fragments arranged by size:
3704 1071 916 85
BsmI GAATG_Cn'
Cuts at: 3287 3380 3287
Size: 93 5683
BsmBI CGTCTCn'nnnn_
Cuts at: 2696 2696
Size: 5776
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2040 2126 2155 2220 2569 2794 2921
Size: 399 250 86 29 65 349 225 127
Cuts at: 2921 3095 3837 3922 4993 1391
Size: 174 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 349 250 225 174
127 86 85 65 29
BspEI T'CCGG_A
Cuts at: 5439 5439
Size: 5776
BspHI T'CATG_A
Cuts at: 3665 5395 3665
Size: 1730 4046
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2181 2568 2681 2897 3074 3131 844
Size: 1337 387 113 216 177 57 3489
Fragments arranged by size:
3489 1337 387 216 177 113 57
BsrBI CCG'CTC
Cuts at: 1394 1823 3663 1394
Size: 429 1840 3507
Fragments arranged by size:
3507 1840 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3044 4271 4445 65
Size: 1183 1796 1227 174 1396
Fragments arranged by size:
1796 1396 1227 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2463 2877 4420 4607 1700
Size: 110 653 414 1543 187 2869
Fragments arranged by size:
2869 1543 653 414 187 110
BsrGI T'GTAC_A
Cuts at: 96 2850 3060 96
Size: 2754 210 2812
Fragments arranged by size:
2812 2754 210
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5776
BssSI C'ACGA_G
Cuts at: 3836 4848 3836
Size: 1012 4764
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2946 3043 794
Size: 2152 97 3527
Fragments arranged by size:
3527 2152 97
BstBI TT'CG_AA
Cuts at: 4580 4580
Size: 5776
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5776
BstXI CCAn_nnnn'nTGG
Cuts at: 2824 2824
Size: 5776
BstYI r'GATC_y
Cuts at: 1972 3106 3473 3858 3875 5327 5413 5425 5771
Size: 1134 367 385 17 1452 86 12 346
Cuts at: 5771 1972
Size: 1977
Fragments arranged by size:
1977 1452 1134 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2237 2621 2237
Size: 384 5392
BtgI C'CryG_G
Cuts at: 513 1065 2641 513
Size: 552 1576 3648
Fragments arranged by size:
3648 1576 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5037
Fragments arranged by size:
5037 683 56
BtsI GCAGTG_nn'
Cuts at: 2124 2171 2483 2998 3017 3292 4086 4106 2124
Size: 47 312 515 19 275 794 20 3794
Fragments arranged by size:
3794 794 515 312 275 47 20 19
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5776
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5741
DraI TTT'AAA
Cuts at: 3080 3080
Size: 5776
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2738 4783 809
Size: 507 1422 2045 1802
Fragments arranged by size:
2045 1802 1422 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2031 3223 4112
Size: 54 1425 50 275 18 201 1192 889
Cuts at: 4112 5741 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1192 889 275 201 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3223 1537
Size: 275 1411 4090
Fragments arranged by size:
4090 1411 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2606 3706 1353
Size: 1253 1100 3423
Fragments arranged by size:
3423 1253 1100
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4363 4737 4883 387
Size: 1064 138 2774 374 146 1280
Fragments arranged by size:
2774 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2180 2198 2330 2477 3839 4436 5223
Size: 393 548 18 132 147 1362 597 787
Cuts at: 5223 5491 1239
Size: 268 1524
Fragments arranged by size:
1524 1362 787 597 548 393 268 147
132 18
EcoO109I rG'GnC_Cy
Cuts at: 2151 2557 2791 2842 2151
Size: 406 234 51 5085
Fragments arranged by size:
5085 406 234 51
EcoRI G'AATT_C
Cuts at: 3085 3463 3085
Size: 378 5398
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2356 2356
Size: 5776
FspI TGC'GCA
Cuts at: 2250 2669 4282 2250
Size: 419 1613 3744
Fragments arranged by size:
3744 1613 419
FspAI rTGC'GCAy
Cuts at: 2669 2669
Size: 5776
HaeII r_GCGC'y
Cuts at: 1678 2054 2296 2415 2431 4923 1678
Size: 376 242 119 16 2492 2531
Fragments arranged by size:
2531 2492 376 242 119 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4417 4449 4491 4523 1373
Size: 32 135 32 2845 32 42 32 2626
Fragments arranged by size:
2845 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3120 3366 669
Size: 988 281 1182 246 3079
Fragments arranged by size:
3079 1182 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5776
HpaI GTT'AAC
Cuts at: 3366 3366
Size: 5776
KpnI G_GTAC'C
Cuts at: 3101 3101
Size: 5776
MfeI C'AATT_G
Cuts at: 3375 3375
Size: 5776
MluI A'CGCG_T
Cuts at: 5652 5652
Size: 5776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4890 5074 1305
Size: 3585 184 2007
Fragments arranged by size:
3585 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3954
Fragments arranged by size:
3954 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3734 4093 4252 518
Size: 648 2568 359 159 2042
Fragments arranged by size:
2568 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2934 3192 3869 5017 5262 1606
Size: 1328 258 677 1148 245 2120
Fragments arranged by size:
2120 1328 1148 677 258 245
NaeI GCC'GGC
Cuts at: 2465 2879 2465
Size: 414 5362
NcoI C'CATG_G
Cuts at: 513 1065 2641 513
Size: 552 1576 3648
Fragments arranged by size:
3648 1576 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5776
NgoMIV G'CCGG_C
Cuts at: 2463 2877 2463
Size: 414 5362
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5776
NotI GC'GGCC_GC
Cuts at: 3223 3223
Size: 5776
NspI r_CATG'y
Cuts at: 1132 1229 3134 4679 1132
Size: 97 1905 1545 2229
Fragments arranged by size:
2229 1905 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5776
PciI A'CATG_T
Cuts at: 4675 4675
Size: 5776
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2395 2837 1108
Size: 1287 442 4047
Fragments arranged by size:
4047 1287 442
PmeI GTTT'AAAC
Cuts at: 3080 3080
Size: 5776
PmlI CAC'GTG
Cuts at: 2593 2631 2593
Size: 38 5738
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3845 3877 5358 5390 3845
Size: 32 1481 32 4231
Fragments arranged by size:
4231 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2557 2842 2557
Size: 285 5491
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5776
PsiI TTA'TAA
Cuts at: 3346 3346
Size: 5776
PspOMI G'GGCC_C
Cuts at: 2151 2790 2151
Size: 639 5137
PstI C_TGCA'G
Cuts at: 830 2411 2939 3128 830
Size: 1581 528 189 3478
Fragments arranged by size:
3478 1581 528 189
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5776
PvuII CAG'CTG
Cuts at: 2934 2934
Size: 5776
SalI G'TCGA_C
Cuts at: 1936 3118 1936
Size: 1182 4594
SbfI CC_TGCA'GG
Cuts at: 3128 3128
Size: 5776
ScaI AGT'ACT
Cuts at: 2891 4024 5736 2891
Size: 1133 1712 2931
Fragments arranged by size:
2931 1712 1133
SexAI A'CCwGG_T
Cuts at: 2844 2844
Size: 5776
SfcI C'TryA_G
Cuts at: 826 1045 2302 2407 2935 3124 4259 4940 5131
Size: 219 1257 105 528 189 1135 681 191
Cuts at: 5131 826
Size: 1471
Fragments arranged by size:
1471 1257 1135 681 528 219 191 189
105
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5776
SmaI CCC'GGG
Cuts at: 1872 2402 2924 3103 1872
Size: 530 522 179 4545
Fragments arranged by size:
4545 530 522 179
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2264 2330 2501 3880 4781 5043
Size: 197 926 321 66 171 1379 901 262
Cuts at: 5043 820
Size: 1553
Fragments arranged by size:
1553 1379 926 901 321 262 197 171
66
SnaBI TAC'GTA
Cuts at: 493 3567 493
Size: 3074 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5776
SphI G_CATG'C
Cuts at: 1132 3134 1132
Size: 2002 3774
SrfI GCCC'GGGC
Cuts at: 2402 2402
Size: 5776
SspI AAT'ATT
Cuts at: 5 52 3700 5
Size: 47 3648 2081
Fragments arranged by size:
3648 2081 47
StuI AGG'CCT
Cuts at: 1517 2471 2768 2823 1517
Size: 954 297 55 4470
Fragments arranged by size:
4470 954 297 55
StyI C'CwwG_G
Cuts at: 513 1065 2641 2769 2831 3215 513
Size: 552 1576 128 62 384 3074
Fragments arranged by size:
3074 1576 552 384 128 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2402 2881 2984 3803 3820 3973 4158
Size: 1255 822 479 103 819 17 153 185
Cuts at: 4158 4603 325
Size: 445 1498
Fragments arranged by size:
1498 1255 822 819 479 445 185 153
103 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2505 2850 2889
Size: 274 80 33 51 1443 528 345 39
Cuts at: 2889 3060 4022 5734 96
Size: 171 962 1712 138
Fragments arranged by size:
1712 1443 962 528 345 274 171 138
80 51 39 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5736 5768 5736
Size: 32 5744
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5776
XbaI T'CTAG_A
Cuts at: 3112 3112
Size: 5776
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2767 2767
Size: 5776
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5776
XmaI C'CCGG_G
Cuts at: 1870 2400 2922 3101 1870
Size: 530 522 179 4545
Fragments arranged by size:
4545 530 522 179
XmnI GAAnn'nnTTC
Cuts at: 3905 3905
Size: 5776
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3584 276
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI
KasI NarI NruI NsiI PacI PfoI PspXI PsrI
RsrII SacI SacII SanDI SapI SfiI SfoI SwaI