Restriction map for
pFN21AE1834
(Circular) (Six-base) MAPSORT of: pFN21AE1834.seq Check: 3866 from: 1 to: 5761
pFN21AE1834 5761 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3571 278
Size: 53 83 186 936 2035 2468
Fragments arranged by size:
2468 2035 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2749 3104 1937
Size: 812 355 4594
Fragments arranged by size:
4594 812 355
Acc65I G'GTAC_C
Cuts at: 3082 3082
Size: 5761
AclI AA'CG_TT
Cuts at: 1563 3888 4261 1563
Size: 2325 373 3063
Fragments arranged by size:
3063 2325 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2577 3824 5208 5476 1632
Size: 945 1247 1384 268 1917
Fragments arranged by size:
1917 1384 1247 945 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5564
AflIII A'CryG_T
Cuts at: 1203 4660 5637 1203
Size: 3457 977 1327
Fragments arranged by size:
3457 1327 977
AgeI A'CCGG_T
Cuts at: 4592 4592
Size: 5761
AhdI GACnn_n'nnGTC
Cuts at: 4490 4490
Size: 5761
AleI CACnn'nnGTG
Cuts at: 1166 2894 1166
Size: 1728 4033
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5761
AlwNI CAG_nnn'CTG
Cuts at: 2509 2532 3504 5076 2509
Size: 23 972 1572 3194
Fragments arranged by size:
3194 1572 972 23
ApaI G_GGCC'C
Cuts at: 2100 2100
Size: 5761
ApaLI G'TGCA_C
Cuts at: 3818 4974 3818
Size: 1156 4605
ApoI r'AATT_y
Cuts at: 1493 3070 3296 3448 5557 1493
Size: 1577 226 152 2109 1697
Fragments arranged by size:
2109 1697 1577 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4315 160
Size: 872 3283 1606
Fragments arranged by size:
3283 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
AvaI C'yCGr_G
Cuts at: 1870 1943 2673 3086 1870
Size: 73 730 413 4545
Fragments arranged by size:
4545 730 413 73
AvrII C'CTAG_G
Cuts at: 2548 2548
Size: 5761
BamHI G'GATC_C
Cuts at: 3091 3458 3091
Size: 367 5394
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2583 2930 2970 3082 4537
Size: 325 217 627 796 347 40 112 1455
Cuts at: 4537 618
Size: 1842
Fragments arranged by size:
1842 1455 796 627 347 325 217 112
40
BanII G_rGCy'C
Cuts at: 1391 2100 2630 2948 3080 1391
Size: 709 530 318 132 4072
Fragments arranged by size:
4072 709 530 318 132
BbeI G_GCGC'C
Cuts at: 2974 2974
Size: 5761
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4790
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3952 3986 1903
Size: 34 2015 34 3678
Fragments arranged by size:
3678 2015 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3653 4863 3653
Size: 1210 4551
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5761
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4372 136
Size: 107 122 71 631 605 2700 1525
Fragments arranged by size:
2700 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5756 5756
Size: 5761
BlpI GC'TnA_GC
Cuts at: 3178 3178
Size: 5761
Bme1580I G_kGCm'C
Cuts at: 1790 2100 2263 2586 2846 3822 4978 1790
Size: 310 163 323 260 976 1156 2573
Fragments arranged by size:
2573 1156 976 323 310 260 163
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5761
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3492 4450 442
Size: 416 53 83 399 24 2075 958 1753
Fragments arranged by size:
2075 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5761
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2132 2534 2615 2958 4421 1239
Size: 893 402 81 343 1463 2579
Fragments arranged by size:
2579 1463 893 402 343 81
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2547 2894 3886 4751 5049 2547
Size: 347 992 865 298 3259
Fragments arranged by size:
3259 992 865 347 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4424 882
Size: 617 2925 2219
Fragments arranged by size:
2925 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3552 493
Size: 711 2348 2702
Fragments arranged by size:
2702 2348 711
BsaBI GATnn'nnATC
Cuts at: 5755 5755
Size: 5761
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2971 3568 3950 4585
Size: 53 83 186 936 1438 597 382 635
Cuts at: 4585 275
Size: 1451
Fragments arranged by size:
1451 1438 936 635 597 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4194 4592 4866 5013 5424 4194
Size: 398 274 147 411 4531
Fragments arranged by size:
4531 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5536
Fragments arranged by size:
5536 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2002 2024 2350 1186
Size: 816 22 326 4597
Fragments arranged by size:
4597 816 326 22
BseYI C'CCAG_C
Cuts at: 2178 2467 4964 5460 2178
Size: 289 2497 496 2479
Fragments arranged by size:
2497 2479 496 289
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2153 2211 2438 3076 1163
Size: 573 417 58 227 638 3848
Fragments arranged by size:
3848 638 573 417 227 58
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3211 3972 5000 1540
Size: 275 186 1210 761 1028 2301
Fragments arranged by size:
2301 1210 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2630 2901 3822 3907 4978 2630
Size: 271 921 85 1071 3413
Fragments arranged by size:
3413 1071 921 271 85
BsmI GAATG_Cn'
Cuts at: 3272 3365 3272
Size: 93 5668
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2263 2586 2630 2846 2901 2948
Size: 399 310 163 323 44 216 55 47
Cuts at: 2948 3080 3822 3907 4978 1391
Size: 132 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 323 310 216 163
132 85 55 47 44
BspEI T'CCGG_A
Cuts at: 5424 5424
Size: 5761
BspHI T'CATG_A
Cuts at: 3650 5380 3650
Size: 1730 4031
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2517 3116 844
Size: 1673 599 3489
Fragments arranged by size:
3489 1673 599
BsrBI CCG'CTC
Cuts at: 1394 1823 3648 1394
Size: 429 1825 3507
Fragments arranged by size:
3507 1825 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2015 4256 4430 65
Size: 1183 767 2241 174 1396
Fragments arranged by size:
2241 1396 1183 767 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4405 4592 1700
Size: 110 2595 187 2869
Fragments arranged by size:
2869 2595 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5761
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5761
BssSI C'ACGA_G
Cuts at: 3821 4833 3821
Size: 1012 4749
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2721 794
Size: 1927 3834
BstBI TT'CG_AA
Cuts at: 4565 4565
Size: 5761
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5761
BstYI r'GATC_y
Cuts at: 1972 3091 3458 3843 3860 5312 5398 5410 5756
Size: 1119 367 385 17 1452 86 12 346
Cuts at: 5756 1972
Size: 1977
Fragments arranged by size:
1977 1452 1119 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2808 2808
Size: 5761
BtgI C'CryG_G
Cuts at: 513 1065 2242 2961 513
Size: 552 1177 719 3313
Fragments arranged by size:
3313 1177 719 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5022
Fragments arranged by size:
5022 683 56
BtsI GCAGTG_nn'
Cuts at: 2228 2846 2889 2901 3064 3277 4071 4091 2228
Size: 618 43 12 163 213 794 20 3898
Fragments arranged by size:
3898 794 618 213 163 43 20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5761
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2034 2069 529
Size: 35 1470 35 4221
Fragments arranged by size:
4221 1470 35 35
DraI TTT'AAA
Cuts at: 3065 3065
Size: 5761
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2387 4768 809
Size: 507 1071 2381 1802
Fragments arranged by size:
2381 1802 1071 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2142 3208
Size: 54 1425 50 275 18 225 87 1066
Cuts at: 3208 4097 5726 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1066 889 275 225 87 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3208 1537
Size: 275 1396 4090
Fragments arranged by size:
4090 1396 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3691 1353
Size: 2338 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4348 4722 4868 387
Size: 1064 138 2759 374 146 1280
Fragments arranged by size:
2759 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2628 2628
Size: 5761
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2132 2534 2577 2615 2958 3824 4421
Size: 393 500 402 43 38 343 866 597
Cuts at: 4421 5208 5476 1239
Size: 787 268 1524
Fragments arranged by size:
1524 866 787 597 500 402 393 343
268 43 38
EcoNI CCTnn'n_nnAGG
Cuts at: 2513 2513
Size: 5761
EcoO109I rG'GnC_Cy
Cuts at: 2096 2248 2552 2096
Size: 152 304 5305
Fragments arranged by size:
5305 304 152
EcoRI G'AATT_C
Cuts at: 3070 3448 3070
Size: 378 5383
FspI TGC'GCA
Cuts at: 4267 4267
Size: 5761
HaeII r_GCGC'y
Cuts at: 1678 2185 2974 4908 1678
Size: 507 789 1934 2531
Fragments arranged by size:
2531 1934 789 507
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2700 2731 2732 2763 4402
Size: 32 135 32 1128 31 1 31 1639
Cuts at: 4402 4434 4476 4508 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1639 1128 135 42 32 32 32
32 31 31 1
HincII GTy'rAC
Cuts at: 669 1657 1938 2750 3105 3351 669
Size: 988 281 812 355 246 3079
Fragments arranged by size:
3079 988 812 355 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5761
HpaI GTT'AAC
Cuts at: 3351 3351
Size: 5761
KasI G'GCGC_C
Cuts at: 2970 2970
Size: 5761
KpnI G_GTAC'C
Cuts at: 3086 3086
Size: 5761
MfeI C'AATT_G
Cuts at: 3360 3360
Size: 5761
MluI A'CGCG_T
Cuts at: 5637 5637
Size: 5761
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2069 4875 5059 1305
Size: 764 2806 184 2007
Fragments arranged by size:
2806 2007 764 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2057 2144 10
Size: 54 1425 343 225 87 3627
Fragments arranged by size:
3627 1425 343 225 87 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2894 3719 4078 4237 518
Size: 648 1728 825 359 159 2042
Fragments arranged by size:
2042 1728 825 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2122 2924 3177 3854 5002 5247 1606
Size: 516 802 253 677 1148 245 2120
Fragments arranged by size:
2120 1148 802 677 516 253 245
NarI GG'CG_CC
Cuts at: 2971 2971
Size: 5761
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5209
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5761
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5761
NotI GC'GGCC_GC
Cuts at: 3208 3208
Size: 5761
NspI r_CATG'y
Cuts at: 1132 1229 2091 2866 3119 4664 1132
Size: 97 862 775 253 1545 2229
Fragments arranged by size:
2229 1545 862 775 253 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5761
PciI A'CATG_T
Cuts at: 4660 4660
Size: 5761
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2090 2532 1108
Size: 982 442 4337
Fragments arranged by size:
4337 982 442
PfoI T'CCnGG_A
Cuts at: 2735 2735
Size: 5761
PmeI GTTT'AAAC
Cuts at: 3065 3065
Size: 5761
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3830 3862 5343 5375 3830
Size: 32 1481 32 4216
Fragments arranged by size:
4216 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2248 2248
Size: 5761
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5761
PsiI TTA'TAA
Cuts at: 3331 3331
Size: 5761
PspOMI G'GGCC_C
Cuts at: 2096 2096
Size: 5761
PstI C_TGCA'G
Cuts at: 830 2235 2576 2896 2929 3113 830
Size: 1405 341 320 33 184 3478
Fragments arranged by size:
3478 1405 341 320 184 33
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5761
PvuII CAG'CTG
Cuts at: 2924 2924
Size: 5761
SacI G_AGCT'C
Cuts at: 2630 2630
Size: 5761
SalI G'TCGA_C
Cuts at: 1936 2748 3103 1936
Size: 812 355 4594
Fragments arranged by size:
4594 812 355
SanDI GG'GwC_CC
Cuts at: 2248 2248
Size: 5761
SbfI CC_TGCA'GG
Cuts at: 3113 3113
Size: 5761
ScaI AGT'ACT
Cuts at: 4009 5721 4009
Size: 1712 4049
SfcI C'TryA_G
Cuts at: 826 1045 2231 2572 2892 2925 3109 4244 4925
Size: 219 1186 341 320 33 184 1135 681
Cuts at: 4925 5116 826
Size: 191 1471
Fragments arranged by size:
1471 1186 1135 681 341 320 219 191
184 33
SfoI GGC'GCC
Cuts at: 2972 2972
Size: 5761
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5761
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5761
SmaI CCC'GGG
Cuts at: 1872 2675 3088 1872
Size: 803 413 4545
Fragments arranged by size:
4545 803 413
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2562 2909 3865 4766 5028 820
Size: 197 926 619 347 956 901 262 1553
Fragments arranged by size:
1553 956 926 901 619 347 262 197
SnaBI TAC'GTA
Cuts at: 493 3552 493
Size: 3059 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5761
SphI G_CATG'C
Cuts at: 1132 2866 3119 1132
Size: 1734 253 3774
Fragments arranged by size:
3774 1734 253
SspI AAT'ATT
Cuts at: 5 52 3685 5
Size: 47 3633 2081
Fragments arranged by size:
3633 2081 47
StuI AGG'CCT
Cuts at: 1517 2319 2659 3047 1517
Size: 802 340 388 4231
Fragments arranged by size:
4231 802 388 340
StyI C'CwwG_G
Cuts at: 513 1065 2103 2493 2548 2725 3200 513
Size: 552 1038 390 55 177 475 3074
Fragments arranged by size:
3074 1038 552 475 390 177 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2192 2630 3042 3788 3805 3958 4143
Size: 1255 612 438 412 746 17 153 185
Cuts at: 4143 4588 325
Size: 445 1498
Fragments arranged by size:
1498 1255 746 612 445 438 412 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4007 5719 96
Size: 274 80 33 51 1443 2030 1712 138
Fragments arranged by size:
2030 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5721 5753 5721
Size: 32 5729
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5761
XbaI T'CTAG_A
Cuts at: 2266 3097 2266
Size: 831 4930
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2107 2491 2107
Size: 384 5377
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5761
XmaI C'CCGG_G
Cuts at: 1870 2673 3086 1870
Size: 803 413 4545
Fragments arranged by size:
4545 803 413
XmnI GAAnn'nnTTC
Cuts at: 3890 3890
Size: 5761
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3569 276
Size: 53 83 186 936 2035 2468
Fragments arranged by size:
2468 2035 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI
SwaI