Restriction map for
pFN21AE1833
(Circular) (Six-base) MAPSORT of: pFN21AE1833.seq Check: 6639 from: 1 to: 5758
pFN21AE1833 5758 bp
With 182 enzymes: SgfI *
August 16, 2010 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2893 2893
Size: 5758
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3568 278
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3101 1937
Size: 1164 4594
Acc65I G'GTAC_C
Cuts at: 3079 3079
Size: 5758
AclI AA'CG_TT
Cuts at: 1563 3885 4258 1563
Size: 2322 373 3063
Fragments arranged by size:
3063 2322 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2198 3821 5205 5473 1632
Size: 566 1623 1384 268 1917
Fragments arranged by size:
1917 1623 1384 566 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5561
AflIII A'CryG_T
Cuts at: 1203 4657 5634 1203
Size: 3454 977 1327
Fragments arranged by size:
3454 1327 977
AgeI A'CCGG_T
Cuts at: 4589 4589
Size: 5758
AhdI GACnn_n'nnGTC
Cuts at: 4487 4487
Size: 5758
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5758
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5758
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2153 2185 2153
Size: 32 5726
AlwNI CAG_nnn'CTG
Cuts at: 2650 3040 3501 5073 2650
Size: 390 461 1572 3335
Fragments arranged by size:
3335 1572 461 390
ApaLI G'TGCA_C
Cuts at: 2884 3815 4971 2884
Size: 931 1156 3671
Fragments arranged by size:
3671 1156 931
ApoI r'AATT_y
Cuts at: 1493 2414 2603 2948 3067 3293 3445 5554 1493
Size: 921 189 345 119 226 152 2109 1697
Fragments arranged by size:
2109 1697 921 345 226 189 152 119
AseI AT'TA_AT
Cuts at: 160 1032 4312 160
Size: 872 3280 1606
Fragments arranged by size:
3280 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
AvaI C'yCGr_G
Cuts at: 1870 1943 3083 1870
Size: 73 1140 4545
Fragments arranged by size:
4545 1140 73
BamHI G'GATC_C
Cuts at: 3088 3455 3088
Size: 367 5391
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2141 2710 2877 3079 4534
Size: 325 217 627 354 569 167 202 1455
Cuts at: 4534 618
Size: 1842
Fragments arranged by size:
1842 1455 627 569 354 325 217 202
167
BanII G_rGCy'C
Cuts at: 1391 2479 3077 1391
Size: 1088 598 4072
Fragments arranged by size:
4072 1088 598
BbeI G_GCGC'C
Cuts at: 2881 2881
Size: 5758
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4787
BbvCI CC'TCA_GC
Cuts at: 2718 2718
Size: 5758
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3949 3983 1903
Size: 34 2012 34 3678
Fragments arranged by size:
3678 2012 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3650 4860 3650
Size: 1210 4548
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5758
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4369 136
Size: 107 122 71 631 605 2697 1525
Fragments arranged by size:
2697 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5753 5753
Size: 5758
BlpI GC'TnA_GC
Cuts at: 3175 3175
Size: 5758
Bme1580I G_kGCm'C
Cuts at: 1790 2146 2715 2888 3034 3819 4975 1790
Size: 356 569 173 146 785 1156 2573
Fragments arranged by size:
2573 1156 785 569 356 173 146
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5758
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2716 3028 3489
Size: 416 53 83 399 24 1299 312 461
Cuts at: 3489 4447 442
Size: 958 1753
Fragments arranged by size:
1753 1299 958 461 416 399 312 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5758
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2307 4418 1239
Size: 1068 2111 2579
Fragments arranged by size:
2579 2111 1068
Bpu10I CC'TnA_GC
Cuts at: 2718 2718
Size: 5758
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3883 4748 5046 3883
Size: 865 298 4595
Fragments arranged by size:
4595 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4421 882
Size: 617 2922 2219
Fragments arranged by size:
2922 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3549 493
Size: 711 2345 2702
Fragments arranged by size:
2702 2345 711
BsaBI GATnn'nnATC
Cuts at: 5752 5752
Size: 5758
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2878 3565 3947 4582
Size: 53 83 186 936 1345 687 382 635
Cuts at: 4582 275
Size: 1451
Fragments arranged by size:
1451 1345 936 687 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4191 4589 4863 5010 5421 4191
Size: 398 274 147 411 4528
Fragments arranged by size:
4528 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2153 2183 1178
Size: 30 165 30 750 30 4753
Fragments arranged by size:
4753 750 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2174 1186
Size: 988 4770
BseYI C'CCAG_C
Cuts at: 2684 4961 5457 2684
Size: 2277 496 2985
Fragments arranged by size:
2985 2277 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5185
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3208 3969 4997 1540
Size: 275 186 1207 761 1028 2301
Fragments arranged by size:
2301 1207 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2888 3819 3904 4975 2888
Size: 931 85 1071 3671
Fragments arranged by size:
3671 1071 931 85
BsmI GAATG_Cn'
Cuts at: 2054 3269 3362 2054
Size: 1215 93 4450
Fragments arranged by size:
4450 1215 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2146 2479 2715 2888 3034 3077 3819
Size: 399 356 333 236 173 146 43 742
Cuts at: 3819 3904 4975 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 356 333 236 173
146 85 43
BspEI T'CCGG_A
Cuts at: 5421 5421
Size: 5758
BspHI T'CATG_A
Cuts at: 3647 5377 3647
Size: 1730 4028
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2792 2893 3113 844
Size: 1948 101 220 3489
Fragments arranged by size:
3489 1948 220 101
BsrBI CCG'CTC
Cuts at: 1394 1823 3645 1394
Size: 429 1822 3507
Fragments arranged by size:
3507 1822 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4253 4427 65
Size: 1183 3005 174 1396
Fragments arranged by size:
3005 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2504 4402 4589 1700
Size: 110 694 1898 187 2869
Fragments arranged by size:
2869 1898 694 187 110
BsrGI T'GTAC_A
Cuts at: 96 2295 96
Size: 2199 3559
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5758
BssSI C'ACGA_G
Cuts at: 3818 4830 3818
Size: 1012 4746
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2060 794
Size: 1266 4492
BstBI TT'CG_AA
Cuts at: 2331 4562 2331
Size: 2231 3527
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5758
BstYI r'GATC_y
Cuts at: 1972 2385 2427 3088 3455 3840 3857 5309 5395
Size: 413 42 661 367 385 17 1452 86
Cuts at: 5395 5407 5753 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 661 413 385 367 346 86
42 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2377 513
Size: 552 939 373 3894
Fragments arranged by size:
3894 939 552 373
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5019
Fragments arranged by size:
5019 683 56
BtsI GCAGTG_nn'
Cuts at: 2523 3034 3058 3274 4068 4088 2523
Size: 511 24 216 794 20 4193
Fragments arranged by size:
4193 794 511 216 24 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5758
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5723
DraI TTT'AAA
Cuts at: 3062 3062
Size: 5758
DraIII CAC_nnn'GTG
Cuts at: 2710 2892 2710
Size: 182 5576
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4765 809
Size: 507 3449 1802
Fragments arranged by size:
3449 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3205 4094 5723
Size: 54 1425 50 275 18 1375 889 1629
Cuts at: 5723 8
Size: 43
Fragments arranged by size:
1629 1425 1375 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3205 1537
Size: 275 1393 4090
Fragments arranged by size:
4090 1393 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2217 2904 3688 1353
Size: 864 687 784 3423
Fragments arranged by size:
3423 864 784 687
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2759 4345 4719 4865 387
Size: 1064 138 1170 1586 374 146 1280
Fragments arranged by size:
1586 1280 1170 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2198 2307 3821 4418 5205 5473 1239
Size: 393 566 109 1514 597 787 268 1524
Fragments arranged by size:
1524 1514 787 597 566 393 268 109
EcoNI CCTnn'n_nnAGG
Cuts at: 3044 3044
Size: 5758
EcoO109I rG'GnC_Cy
Cuts at: 2099 2099
Size: 5758
EcoRI G'AATT_C
Cuts at: 2603 3067 3445 2603
Size: 464 378 4916
Fragments arranged by size:
4916 464 378
FspI TGC'GCA
Cuts at: 3013 4264 3013
Size: 1251 4507
HaeII r_GCGC'y
Cuts at: 1678 2651 2697 2881 4905 1678
Size: 973 46 184 2024 2531
Fragments arranged by size:
2531 2024 973 184 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2083 2115 4399 4431 4473
Size: 32 135 32 511 32 2284 32 42
Cuts at: 4473 4505 1373
Size: 32 2626
Fragments arranged by size:
2626 2284 511 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3102 3348 669
Size: 988 281 1164 246 3079
Fragments arranged by size:
3079 1164 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5758
HpaI GTT'AAC
Cuts at: 3348 3348
Size: 5758
KasI G'GCGC_C
Cuts at: 2877 2877
Size: 5758
KpnI G_GTAC'C
Cuts at: 3083 3083
Size: 5758
MfeI C'AATT_G
Cuts at: 3357 3357
Size: 5758
MluI A'CGCG_T
Cuts at: 5634 5634
Size: 5758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4872 5056 1305
Size: 3567 184 2007
Fragments arranged by size:
3567 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3936
Fragments arranged by size:
3936 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2545 2854 3716 4075 4234 518
Size: 648 1379 309 862 359 159 2042
Fragments arranged by size:
2042 1379 862 648 359 309 159
MspA1I CmG'CkG
Cuts at: 1606 2596 2677 2717 2937 3174 3851 4999 5244
Size: 990 81 40 220 237 677 1148 245
Cuts at: 5244 1606
Size: 2120
Fragments arranged by size:
2120 1148 990 677 245 237 220 81
40
NaeI GCC'GGC
Cuts at: 2506 2506
Size: 5758
NarI GG'CG_CC
Cuts at: 2878 2878
Size: 5758
NcoI C'CATG_G
Cuts at: 513 1065 2004 2377 513
Size: 552 939 373 3894
Fragments arranged by size:
3894 939 552 373
NdeI CA'TA_TG
Cuts at: 387 2042 387
Size: 1655 4103
NgoMIV G'CCGG_C
Cuts at: 2504 2504
Size: 5758
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5758
NotI GC'GGCC_GC
Cuts at: 3205 3205
Size: 5758
NspI r_CATG'y
Cuts at: 1132 1229 2273 2544 2586 2993 3116 4661 1132
Size: 97 1044 271 42 407 123 1545 2229
Fragments arranged by size:
2229 1545 1044 407 271 123 97 42
PasI CC'CwG_GG
Cuts at: 1348 3040 1348
Size: 1692 4066
PciI A'CATG_T
Cuts at: 4657 4657
Size: 5758
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3040 1108
Size: 1932 3826
PfoI T'CCnGG_A
Cuts at: 2607 2607
Size: 5758
PmeI GTTT'AAAC
Cuts at: 3062 3062
Size: 5758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2153 2185 3827 3859 5340 5372 2153
Size: 32 1642 32 1481 32 2539
Fragments arranged by size:
2539 1642 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2099 2099
Size: 5758
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5758
PsiI TTA'TAA
Cuts at: 3328 3328
Size: 5758
PstI C_TGCA'G
Cuts at: 830 2402 2573 2595 2904 3110 830
Size: 1572 171 22 309 206 3478
Fragments arranged by size:
3478 1572 309 206 171 22
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5758
PvuII CAG'CTG
Cuts at: 2596 2677 2937 2596
Size: 81 260 5417
Fragments arranged by size:
5417 260 81
SalI G'TCGA_C
Cuts at: 1936 3100 1936
Size: 1164 4594
SapI GCTCTTCn'nnn_
Cuts at: 2217 2217
Size: 5758
SbfI CC_TGCA'GG
Cuts at: 3110 3110
Size: 5758
ScaI AGT'ACT
Cuts at: 4006 5718 4006
Size: 1712 4046
SfcI C'TryA_G
Cuts at: 826 1045 2314 2398 2569 2591 2831 2900 3106
Size: 219 1269 84 171 22 240 69 206
Cuts at: 3106 4241 4922 5113 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1269 1135 681 240 219 206 191
171 84 69 22
SfoI GGC'GCC
Cuts at: 2879 2879
Size: 5758
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5758
SmaI CCC'GGG
Cuts at: 1872 3085 1872
Size: 1213 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3862 4763 5025 820
Size: 197 926 1919 901 262 1553
Fragments arranged by size:
1919 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3549 493
Size: 3056 2702
SpeI A'CTAG_T
Cuts at: 152 2126 152
Size: 1974 3784
SphI G_CATG'C
Cuts at: 1132 3116 1132
Size: 1984 3774
SspI AAT'ATT
Cuts at: 5 52 3682 5
Size: 47 3630 2081
Fragments arranged by size:
3630 2081 47
StuI AGG'CCT
Cuts at: 1517 2108 2723 1517
Size: 591 615 4552
Fragments arranged by size:
4552 615 591
StyI C'CwwG_G
Cuts at: 513 1065 2004 2103 2136 2377 3197 513
Size: 552 939 99 33 241 820 3074
Fragments arranged by size:
3074 939 820 552 241 99 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2129 2240 2312 2698 3785 3802 3955
Size: 1255 549 111 72 386 1087 17 153
Cuts at: 3955 4140 4585 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1087 549 445 386 185 153
111 72 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2295 4004 5716
Size: 274 80 33 51 1443 318 1709 1712
Cuts at: 5716 96
Size: 138
Fragments arranged by size:
1712 1709 1443 318 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2834 2866 5718 5750 2834
Size: 32 2852 32 2842
Fragments arranged by size:
2852 2842 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5758
XbaI T'CTAG_A
Cuts at: 3094 3094
Size: 5758
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2440 2440
Size: 5758
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5758
XmaI C'CCGG_G
Cuts at: 1870 3083 1870
Size: 1213 4545
XmnI GAAnn'nnTTC
Cuts at: 2084 3887 2084
Size: 1803 3955
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3566 276
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI
FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI