Restriction map for
pFN21AE1832
(Circular) (Six-base) MAPSORT of: pFN21AE1832.seq Check: 6481 from: 1 to: 5758
pFN21AE1832 5758 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2152 2348 2950 2152
Size: 196 602 4960
Fragments arranged by size:
4960 602 196
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2903 3568 278
Size: 53 83 186 936 1367 665 2468
Fragments arranged by size:
2468 1367 936 665 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3101 1937
Size: 1164 4594
Acc65I G'GTAC_C
Cuts at: 3079 3079
Size: 5758
AclI AA'CG_TT
Cuts at: 1563 3885 4258 1563
Size: 2322 373 3063
Fragments arranged by size:
3063 2322 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2726 3821 5205 5473 1632
Size: 1094 1095 1384 268 1917
Fragments arranged by size:
1917 1384 1095 1094 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5561
AflIII A'CryG_T
Cuts at: 1203 3048 4657 5634 1203
Size: 1845 1609 977 1327
Fragments arranged by size:
1845 1609 1327 977
AgeI A'CCGG_T
Cuts at: 4589 4589
Size: 5758
AhdI GACnn_n'nnGTC
Cuts at: 4487 4487
Size: 5758
AleI CACnn'nnGTG
Cuts at: 1166 2732 1166
Size: 1566 4192
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2882 1184
Size: 1698 4060
AlwNI CAG_nnn'CTG
Cuts at: 3501 5073 3501
Size: 1572 4186
ApaLI G'TGCA_C
Cuts at: 3815 4971 3815
Size: 1156 4602
ApoI r'AATT_y
Cuts at: 1493 2990 3067 3293 3445 5554 1493
Size: 1497 77 226 152 2109 1697
Fragments arranged by size:
2109 1697 1497 226 152 77
AseI AT'TA_AT
Cuts at: 160 1032 4312 160
Size: 872 3280 1606
Fragments arranged by size:
3280 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
AvaI C'yCGr_G
Cuts at: 1870 1943 2322 3083 1870
Size: 73 379 761 4545
Fragments arranged by size:
4545 761 379 73
BamHI G'GATC_C
Cuts at: 3088 3455 3088
Size: 367 5391
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2213 2723 2770 3079 4534
Size: 325 217 627 426 510 47 309 1455
Cuts at: 4534 618
Size: 1842
Fragments arranged by size:
1842 1455 627 510 426 325 309 217
47
BanII G_rGCy'C
Cuts at: 1391 2557 2756 3077 1391
Size: 1166 199 321 4072
Fragments arranged by size:
4072 1166 321 199
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2038 2047 2287 928
Size: 971 139 9 240 4399
Fragments arranged by size:
4399 971 240 139 9
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2303 2337 3949 3983 1903
Size: 34 366 34 1612 34 3678
Fragments arranged by size:
3678 1612 366 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3650 4860 3650
Size: 1210 4548
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5758
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2222 2448 2751
Size: 107 122 71 631 605 550 226 303
Cuts at: 2751 4369 136
Size: 1618 1525
Fragments arranged by size:
1618 1525 631 605 550 303 226 122
107 71
BglII A'GATC_T
Cuts at: 5753 5753
Size: 5758
BlpI GC'TnA_GC
Cuts at: 3175 3175
Size: 5758
Bme1580I G_kGCm'C
Cuts at: 1790 2218 2767 2876 3819 4975 1790
Size: 428 549 109 943 1156 2573
Fragments arranged by size:
2573 1156 943 549 428 109
BmgBI CAC'GTC
Cuts at: 1318 2130 2661 1318
Size: 812 531 4415
Fragments arranged by size:
4415 812 531
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3489 4447 442
Size: 416 53 83 399 24 2072 958 1753
Fragments arranged by size:
2072 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5758
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2423 2743 4418 1239
Size: 1184 320 1675 2579
Fragments arranged by size:
2579 1675 1184 320
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2126 3883 4748 5046 2126
Size: 1757 865 298 2838
Fragments arranged by size:
2838 1757 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4421 882
Size: 617 2922 2219
Fragments arranged by size:
2922 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3051 3549 493
Size: 711 1847 498 2702
Fragments arranged by size:
2702 1847 711 498
BsaBI GATnn'nnATC
Cuts at: 5752 5752
Size: 5758
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2900 3565 3947 4582
Size: 53 83 186 936 1367 665 382 635
Cuts at: 4582 275
Size: 1451
Fragments arranged by size:
1451 1367 936 665 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2250 4191 4589 4863 5010 5421 2250
Size: 1941 398 274 147 411 2587
Fragments arranged by size:
2587 1941 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2114 2144 2225 2255 1178
Size: 30 165 30 711 30 81 30 4681
Fragments arranged by size:
4681 711 165 81 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 2135 2138 2246 2746 1186
Size: 844 105 3 108 500 4198
Fragments arranged by size:
4198 844 500 108 105 3
BseYI C'CCAG_C
Cuts at: 2217 2869 4961 5457 2217
Size: 652 2092 496 2518
Fragments arranged by size:
2518 2092 652 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2186 2407 1163
Size: 573 450 221 4514
Fragments arranged by size:
4514 573 450 221
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3208 3969 4997 1540
Size: 275 186 1207 761 1028 2301
Fragments arranged by size:
2301 1207 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3819 3904 4975 3819
Size: 85 1071 4602
Fragments arranged by size:
4602 1071 85
BsmI GAATG_Cn'
Cuts at: 2624 3269 3362 2624
Size: 645 93 5020
Fragments arranged by size:
5020 645 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2120 2120
Size: 5758
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2218 2557 2756 2767 2876 3077 3819
Size: 399 428 339 199 11 109 201 742
Cuts at: 3819 3904 4975 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 428 399 339 201 199
109 85 11
BspEI T'CCGG_A
Cuts at: 2250 5421 2250
Size: 3171 2587
BspHI T'CATG_A
Cuts at: 2559 3647 5377 2559
Size: 1088 1730 2940
Fragments arranged by size:
2940 1730 1088
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2152 2348 2950 3113 844
Size: 1308 196 602 163 3489
Fragments arranged by size:
3489 1308 602 196 163
BsrBI CCG'CTC
Cuts at: 1394 1823 2016 3645 1394
Size: 429 193 1629 3507
Fragments arranged by size:
3507 1629 429 193
BsrDI GCAATG_nn'
Cuts at: 65 1248 4253 4427 65
Size: 1183 3005 174 1396
Fragments arranged by size:
3005 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2256 2582 3018 4402 4589 1700
Size: 110 446 326 436 1384 187 2869
Fragments arranged by size:
2869 1384 446 436 326 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5758
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5758
BssSI C'ACGA_G
Cuts at: 2017 2875 3035 3818 4830 2017
Size: 858 160 783 1012 2945
Fragments arranged by size:
2945 1012 858 783 160
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2854 794
Size: 2060 3698
BstBI TT'CG_AA
Cuts at: 4562 4562
Size: 5758
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5758
BstXI CCAn_nnnn'nTGG
Cuts at: 2734 2767 2734
Size: 33 5725
BstYI r'GATC_y
Cuts at: 1972 3088 3455 3840 3857 5309 5395 5407 5753
Size: 1116 367 385 17 1452 86 12 346
Cuts at: 5753 1972
Size: 1977
Fragments arranged by size:
1977 1452 1116 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2209 2629 2941 513
Size: 552 1144 420 312 3330
Fragments arranged by size:
3330 1144 552 420 312
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2464 487
Size: 683 56 1238 3781
Fragments arranged by size:
3781 1238 683 56
BtsI GCAGTG_nn'
Cuts at: 2379 2398 3029 3274 4068 4088 2379
Size: 19 631 245 794 20 4049
Fragments arranged by size:
4049 794 631 245 20 19
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5758
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5723
DraI TTT'AAA
Cuts at: 3062 3062
Size: 5758
DraIII CAC_nnn'GTG
Cuts at: 2635 2635
Size: 5758
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4765 809
Size: 507 3449 1802
Fragments arranged by size:
3449 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2088 2223 2938
Size: 54 1425 50 275 18 258 135 715
Cuts at: 2938 3205 4094 5723 8
Size: 267 889 1629 43
Fragments arranged by size:
1629 1425 889 715 275 267 258 135
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3205 1537
Size: 275 1393 4090
Fragments arranged by size:
4090 1393 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2356 3688 1353
Size: 1003 1332 3423
Fragments arranged by size:
3423 1332 1003
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2675 4345 4719 4865 387
Size: 1064 138 1086 1670 374 146 1280
Fragments arranged by size:
1670 1280 1086 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2423 2726 2743 3821 4418 5205 5473
Size: 393 791 303 17 1078 597 787 268
Cuts at: 5473 1239
Size: 1524
Fragments arranged by size:
1524 1078 791 787 597 393 303 268
17
EcoO109I rG'GnC_Cy
Cuts at: 2146 2538 2809 2146
Size: 392 271 5095
Fragments arranged by size:
5095 392 271
EcoRI G'AATT_C
Cuts at: 3067 3445 3067
Size: 378 5380
FspI TGC'GCA
Cuts at: 2858 4264 2858
Size: 1406 4352
HaeII r_GCGC'y
Cuts at: 1678 2263 4905 1678
Size: 585 2642 2531
Fragments arranged by size:
2642 2531 585
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4399 4431 4473 4505 1373
Size: 32 135 32 2827 32 42 32 2626
Fragments arranged by size:
2827 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3102 3348 669
Size: 988 281 1164 246 3079
Fragments arranged by size:
3079 1164 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5758
HpaI GTT'AAC
Cuts at: 3348 3348
Size: 5758
KpnI G_GTAC'C
Cuts at: 3083 3083
Size: 5758
MfeI C'AATT_G
Cuts at: 3357 3357
Size: 5758
MluI A'CGCG_T
Cuts at: 5634 5634
Size: 5758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4872 5056 1305
Size: 3567 184 2007
Fragments arranged by size:
3567 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2090 2225 10
Size: 54 1425 343 258 135 3543
Fragments arranged by size:
3543 1425 343 258 135 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2574 2612 2732 2802 3716 4075 4234
Size: 648 1408 38 120 70 914 359 159
Cuts at: 4234 518
Size: 2042
Fragments arranged by size:
2042 1408 914 648 359 159 120 70
38
MspA1I CmG'CkG
Cuts at: 1606 2211 2221 2862 2943 2985 3031 3174 3851
Size: 605 10 641 81 42 46 143 677
Cuts at: 3851 4999 5244 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 641 605 245 143 81
46 42 10
NaeI GCC'GGC
Cuts at: 2584 3020 2584
Size: 436 5322
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5206
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5758
NgoMIV G'CCGG_C
Cuts at: 2582 3018 2582
Size: 436 5322
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5758
NotI GC'GGCC_GC
Cuts at: 3205 3205
Size: 5758
NspI r_CATG'y
Cuts at: 1132 1229 2611 2857 2892 3116 4661 1132
Size: 97 1382 246 35 224 1545 2229
Fragments arranged by size:
2229 1545 1382 246 224 97 35
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5758
PciI A'CATG_T
Cuts at: 4657 4657
Size: 5758
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5758
PmeI GTTT'AAAC
Cuts at: 3062 3062
Size: 5758
PmlI CAC'GTG
Cuts at: 3051 3051
Size: 5758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3827 3859 5340 5372 3827
Size: 32 1481 32 4213
Fragments arranged by size:
4213 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2809 2809
Size: 5758
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5758
PsiI TTA'TAA
Cuts at: 3328 3328
Size: 5758
PstI C_TGCA'G
Cuts at: 830 2984 3110 830
Size: 2154 126 3478
Fragments arranged by size:
3478 2154 126
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5758
PvuII CAG'CTG
Cuts at: 2221 2985 2221
Size: 764 4994
SacII CC_GC'GG
Cuts at: 2212 2944 2212
Size: 732 5026
SalI G'TCGA_C
Cuts at: 1936 3100 1936
Size: 1164 4594
SbfI CC_TGCA'GG
Cuts at: 3110 3110
Size: 5758
ScaI AGT'ACT
Cuts at: 4006 5718 4006
Size: 1712 4046
SexAI A'CCwGG_T
Cuts at: 2811 2811
Size: 5758
SfcI C'TryA_G
Cuts at: 826 1045 2980 3106 4241 4922 5113 826
Size: 219 1935 126 1135 681 191 1471
Fragments arranged by size:
1935 1471 1135 681 219 191 126
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5758
SmaI CCC'GGG
Cuts at: 1872 3085 1872
Size: 1213 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2141 3862 4763 5025 820
Size: 197 926 198 1721 901 262 1553
Fragments arranged by size:
1721 1553 926 901 262 198 197
SnaBI TAC'GTA
Cuts at: 493 3549 493
Size: 3056 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5758
SphI G_CATG'C
Cuts at: 1132 3116 1132
Size: 1984 3774
SspI AAT'ATT
Cuts at: 5 52 3682 5
Size: 47 3630 2081
Fragments arranged by size:
3630 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5758
StyI C'CwwG_G
Cuts at: 513 1065 2904 3197 513
Size: 552 1839 293 3074
Fragments arranged by size:
3074 1839 552 293
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3785 3802 3955 4140 4585 325
Size: 1255 2205 17 153 185 445 1498
Fragments arranged by size:
2205 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2817 4004 5716
Size: 274 80 33 51 1443 840 1187 1712
Cuts at: 5716 96
Size: 138
Fragments arranged by size:
1712 1443 1187 840 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2114 2146 2436 2468 2609 2641 5718 5750 2114
Size: 32 290 32 141 32 3077 32 2122
Fragments arranged by size:
3077 2122 290 141 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5758
XbaI T'CTAG_A
Cuts at: 3094 3094
Size: 5758
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5758
XmaI C'CCGG_G
Cuts at: 1870 3083 1870
Size: 1213 4545
XmnI GAAnn'nnTTC
Cuts at: 3887 3887
Size: 5758
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2901 3566 276
Size: 53 83 186 936 1367 665 2468
Fragments arranged by size:
2468 1367 936 665 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacII SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PfoI PspOMI PspXI PsrI RsrII SacI SanDI
SapI SfiI SfoI SrfI SwaI XcmI