Restriction map for
pFN21AE1831
(Circular) (Six-base) MAPSORT of: pFN21AE1831.seq Check: 2244 from: 1 to: 5752
pFN21AE1831 5752 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2691 2704 3026 3053 2691
Size: 13 322 27 5390
Fragments arranged by size:
5390 322 27 13
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3562 278
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3095 1937
Size: 1158 4594
Acc65I G'GTAC_C
Cuts at: 3073 3073
Size: 5752
AclI AA'CG_TT
Cuts at: 1563 3879 4252 1563
Size: 2316 373 3063
Fragments arranged by size:
3063 2316 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2708 3815 5199 5467 1632
Size: 1076 1107 1384 268 1917
Fragments arranged by size:
1917 1384 1107 1076 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5555
AflIII A'CryG_T
Cuts at: 1203 2037 4651 5628 1203
Size: 834 2614 977 1327
Fragments arranged by size:
2614 1327 977 834
AgeI A'CCGG_T
Cuts at: 4583 4583
Size: 5752
AhdI GACnn_n'nnGTC
Cuts at: 4481 4481
Size: 5752
AleI CACnn'nnGTG
Cuts at: 1166 2963 1166
Size: 1797 3955
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5752
AlwNI CAG_nnn'CTG
Cuts at: 3495 5067 3495
Size: 1572 4180
ApaLI G'TGCA_C
Cuts at: 3809 4965 3809
Size: 1156 4596
ApoI r'AATT_y
Cuts at: 1493 2984 3061 3287 3439 5548 1493
Size: 1491 77 226 152 2109 1697
Fragments arranged by size:
2109 1697 1491 226 152 77
AseI AT'TA_AT
Cuts at: 160 1032 4306 160
Size: 872 3274 1606
Fragments arranged by size:
3274 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
AvaI C'yCGr_G
Cuts at: 1870 1943 2511 3077 1870
Size: 73 568 566 4545
Fragments arranged by size:
4545 568 566 73
BamHI G'GATC_C
Cuts at: 3082 3449 3082
Size: 367 5385
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2535 3073 4528 618
Size: 325 217 627 748 538 1455 1842
Fragments arranged by size:
1842 1455 748 627 538 325 217
BanII G_rGCy'C
Cuts at: 1391 2295 3071 1391
Size: 904 776 4072
Fragments arranged by size:
4072 904 776
BbeI G_GCGC'C
Cuts at: 2539 2539
Size: 5752
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2272 2279 3031 928
Size: 971 373 7 752 3649
Fragments arranged by size:
3649 971 752 373 7
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3943 3977 1903
Size: 34 50 34 1922 34 3678
Fragments arranged by size:
3678 1922 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3644 4854 3644
Size: 1210 4542
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5752
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4363 136
Size: 107 122 71 631 605 2691 1525
Fragments arranged by size:
2691 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5747 5747
Size: 5752
BlpI GC'TnA_GC
Cuts at: 3169 3169
Size: 5752
Bme1580I G_kGCm'C
Cuts at: 1790 2870 3813 4969 1790
Size: 1080 943 1156 2573
Fragments arranged by size:
2573 1156 1080 943
BmgBI CAC'GTC
Cuts at: 1318 2470 2895 1318
Size: 1152 425 4175
Fragments arranged by size:
4175 1152 425
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3483 4441 442
Size: 416 53 83 399 24 2066 958 1753
Fragments arranged by size:
2066 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2618 4412 1239
Size: 1379 1794 2579
Fragments arranged by size:
2579 1794 1379
Bpu10I CC'TnA_GC
Cuts at: 2387 2387
Size: 5752
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3877 4742 5040 3877
Size: 865 298 4589
Fragments arranged by size:
4589 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4415 882
Size: 617 2916 2219
Fragments arranged by size:
2916 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2038 2065 2325 3543 493
Size: 711 834 27 260 1218 2702
Fragments arranged by size:
2702 1218 834 711 260 27
BsaBI GATnn'nnATC
Cuts at: 2210 5746 2210
Size: 3536 2216
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2055 2536 3559 3941
Size: 53 83 186 936 522 481 1023 382
Cuts at: 3941 4576 275
Size: 635 1451
Fragments arranged by size:
1451 1023 936 635 522 481 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2586 4185 4583 4857 5004 5415 2586
Size: 1599 398 274 147 411 2923
Fragments arranged by size:
2923 1599 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2591 2621 1178
Size: 30 165 30 1188 30 4309
Fragments arranged by size:
4309 1188 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2855 1186
Size: 1669 4083
BseYI C'CCAG_C
Cuts at: 4955 5451 4955
Size: 496 5256
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2614 2732 1163
Size: 573 878 118 4183
Fragments arranged by size:
4183 878 573 118
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2407 2466 2709 2935 3202 3963
Size: 275 186 406 59 243 226 267 761
Cuts at: 3963 4991 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 406 275 267 243 226
186 59
BsiHKAI G_wGCw'C
Cuts at: 2080 2295 3813 3898 4969 2080
Size: 215 1518 85 1071 2863
Fragments arranged by size:
2863 1518 1071 215 85
BsiWI C'GTAC_G
Cuts at: 2325 2325
Size: 5752
BsmI GAATG_Cn'
Cuts at: 3263 3356 3263
Size: 93 5659
BsmBI CGTCTCn'nnnn_
Cuts at: 2885 2885
Size: 5752
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2080 2295 2870 3071 3813 3898 4969
Size: 399 290 215 575 201 742 85 1071
Cuts at: 4969 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 575 399 290 215 201
85
BspEI T'CCGG_A
Cuts at: 5415 5415
Size: 5752
BspHI T'CATG_A
Cuts at: 2541 3641 5371 2541
Size: 1100 1730 2922
Fragments arranged by size:
2922 1730 1100
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2556 2651 2691 2704 3026 3053 3107 844
Size: 1712 95 40 13 322 27 54 3489
Fragments arranged by size:
3489 1712 322 95 54 40 27 13
BsrBI CCG'CTC
Cuts at: 1394 1823 3639 1394
Size: 429 1816 3507
Fragments arranged by size:
3507 1816 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4247 4421 65
Size: 1183 2999 174 1396
Fragments arranged by size:
2999 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2564 4396 4583 1700
Size: 110 754 1832 187 2869
Fragments arranged by size:
2869 1832 754 187 110
BsrGI T'GTAC_A
Cuts at: 96 2811 96
Size: 2715 3037
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5752
BssSI C'ACGA_G
Cuts at: 3812 4824 3812
Size: 1012 4740
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3018 794
Size: 2224 3528
BstBI TT'CG_AA
Cuts at: 4556 4556
Size: 5752
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5752
BstYI r'GATC_y
Cuts at: 1972 3082 3449 3834 3851 5303 5389 5401 5747
Size: 1110 367 385 17 1452 86 12 346
Cuts at: 5747 1972
Size: 1977
Fragments arranged by size:
1977 1452 1110 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2088 2141 2337 2611 2793 2853 513
Size: 552 1023 53 196 274 182 60 3412
Fragments arranged by size:
3412 1023 552 274 196 182 60 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5013
Fragments arranged by size:
5013 683 56
BtsI GCAGTG_nn'
Cuts at: 2978 3268 4062 4082 2978
Size: 290 794 20 4648
Fragments arranged by size:
4648 794 290 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5752
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5717
DraI TTT'AAA
Cuts at: 3056 3056
Size: 5752
DraIII CAC_nnn'GTG
Cuts at: 2617 2925 2617
Size: 308 5444
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4759 809
Size: 507 3443 1802
Fragments arranged by size:
3443 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2463 2706 2850
Size: 54 1425 50 275 18 633 243 144
Cuts at: 2850 2932 3199 4088 5717 8
Size: 82 267 889 1629 43
Fragments arranged by size:
1629 1425 889 633 275 267 243 144
82 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2463 2706 2932 3199 1537
Size: 275 651 243 226 267 4090
Fragments arranged by size:
4090 651 275 267 243 226
EarI CTCTTCn'nnn_
Cuts at: 1353 3682 1353
Size: 2329 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4339 4713 4859 387
Size: 1064 138 2750 374 146 1280
Fragments arranged by size:
2750 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2293 2293
Size: 5752
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2618 2708 3815 4412 5199 5467 1239
Size: 393 986 90 1107 597 787 268 1524
Fragments arranged by size:
1524 1107 986 787 597 393 268 90
EcoNI CCTnn'n_nnAGG
Cuts at: 2687 2687
Size: 5752
EcoRI G'AATT_C
Cuts at: 3061 3439 3061
Size: 378 5374
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2296 2296
Size: 5752
FspI TGC'GCA
Cuts at: 2762 2777 4258 2762
Size: 15 1481 4256
Fragments arranged by size:
4256 1481 15
HaeII r_GCGC'y
Cuts at: 1678 2500 2539 4899 1678
Size: 822 39 2360 2531
Fragments arranged by size:
2531 2360 822 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2094 2126 4393 4425 4467
Size: 32 135 32 522 32 2267 32 42
Cuts at: 4467 4499 1373
Size: 32 2626
Fragments arranged by size:
2626 2267 522 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3096 3342 669
Size: 988 281 1158 246 3079
Fragments arranged by size:
3079 1158 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5752
HpaI GTT'AAC
Cuts at: 3342 3342
Size: 5752
KasI G'GCGC_C
Cuts at: 2535 2535
Size: 5752
KpnI G_GTAC'C
Cuts at: 3077 3077
Size: 5752
MfeI C'AATT_G
Cuts at: 3351 3351
Size: 5752
MluI A'CGCG_T
Cuts at: 5628 5628
Size: 5752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4866 5050 1305
Size: 3561 184 2007
Fragments arranged by size:
3561 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3930
Fragments arranged by size:
3930 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2183 2556 2963 3710 4069 4228 518
Size: 648 1017 373 407 747 359 159 2042
Fragments arranged by size:
2042 1017 747 648 407 373 359 159
MspA1I CmG'CkG
Cuts at: 1606 2143 2339 2366 2596 2773 3168 3845 4993
Size: 537 196 27 230 177 395 677 1148
Cuts at: 4993 5238 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 537 395 245 230 196
177 27
NaeI GCC'GGC
Cuts at: 2566 2566
Size: 5752
NarI GG'CG_CC
Cuts at: 2536 2536
Size: 5752
NcoI C'CATG_G
Cuts at: 513 1065 2793 513
Size: 552 1728 3472
Fragments arranged by size:
3472 1728 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5752
NgoMIV G'CCGG_C
Cuts at: 2564 2564
Size: 5752
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5752
NotI GC'GGCC_GC
Cuts at: 3199 3199
Size: 5752
NspI r_CATG'y
Cuts at: 1132 1229 3110 4655 1132
Size: 97 1881 1545 2229
Fragments arranged by size:
2229 1881 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2098 1348
Size: 750 5002
PciI A'CATG_T
Cuts at: 4651 4651
Size: 5752
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2967 1108
Size: 1859 3893
PmeI GTTT'AAAC
Cuts at: 3056 3056
Size: 5752
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3821 3853 5334 5366 3821
Size: 32 1481 32 4207
Fragments arranged by size:
4207 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5752
PsiI TTA'TAA
Cuts at: 3322 3322
Size: 5752
PstI C_TGCA'G
Cuts at: 830 2375 3023 3104 830
Size: 1545 648 81 3478
Fragments arranged by size:
3478 1545 648 81
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5752
PvuII CAG'CTG
Cuts at: 2596 2773 2596
Size: 177 5575
SacI G_AGCT'C
Cuts at: 2295 2295
Size: 5752
SacII CC_GC'GG
Cuts at: 2144 2340 2144
Size: 196 5556
SalI G'TCGA_C
Cuts at: 1936 3094 1936
Size: 1158 4594
SbfI CC_TGCA'GG
Cuts at: 3104 3104
Size: 5752
ScaI AGT'ACT
Cuts at: 4000 5712 4000
Size: 1712 4040
SexAI A'CCwGG_T
Cuts at: 2805 2805
Size: 5752
SfcI C'TryA_G
Cuts at: 826 1045 2371 3019 3100 4235 4916 5107 826
Size: 219 1326 648 81 1135 681 191 1471
Fragments arranged by size:
1471 1326 1135 681 648 219 191 81
SfoI GGC'GCC
Cuts at: 2537 2537
Size: 5752
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5752
SmaI CCC'GGG
Cuts at: 1872 3079 1872
Size: 1207 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3856 4757 5019 820
Size: 197 926 1913 901 262 1553
Fragments arranged by size:
1913 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3543 493
Size: 3050 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5752
SphI G_CATG'C
Cuts at: 1132 3110 1132
Size: 1978 3774
SspI AAT'ATT
Cuts at: 5 52 3676 5
Size: 47 3624 2081
Fragments arranged by size:
3624 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5752
StyI C'CwwG_G
Cuts at: 513 1065 2377 2793 2961 3191 513
Size: 552 1312 416 168 230 3074
Fragments arranged by size:
3074 1312 552 416 230 168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2963 3779 3796 3949 4134 4579 325
Size: 1255 1383 816 17 153 185 445 1498
Fragments arranged by size:
1498 1383 1255 816 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2697 2811 2955
Size: 274 80 33 51 1443 720 114 144
Cuts at: 2955 3998 5710 96
Size: 1043 1712 138
Fragments arranged by size:
1712 1443 1043 720 274 144 138 114
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2591 2623 5712 5744 2591
Size: 32 3089 32 2599
Fragments arranged by size:
3089 2599 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5752
XbaI T'CTAG_A
Cuts at: 2578 3088 2578
Size: 510 5242
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2618 2860 2618
Size: 242 5510
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5752
XmaI C'CCGG_G
Cuts at: 1870 3077 1870
Size: 1207 4545
XmnI GAAnn'nnTTC
Cuts at: 3881 3881
Size: 5752
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3560 276
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SacII SalI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
BplI BstXI BstZ17I Bsu36I EcoO109I EcoRV FseI FspAI
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SanDI SapI SfiI SrfI SwaI