Restriction map for
pFN21AE1827
(Circular) (Six-base) MAPSORT of: pFN21AE1827.seq Check: 1189 from: 1 to: 5740
pFN21AE1827 5740 bp
With 182 enzymes: SgfI *
August 16, 2010 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3550 278
Size: 53 83 186 936 2014 2468
Fragments arranged by size:
2468 2014 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2162 3083 1937
Size: 225 921 4594
Fragments arranged by size:
4594 921 225
Acc65I G'GTAC_C
Cuts at: 3061 3061
Size: 5740
AclI AA'CG_TT
Cuts at: 1563 3867 4240 1563
Size: 2304 373 3063
Fragments arranged by size:
3063 2304 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2508 2523 2915 3803 5187 5455 1632
Size: 876 15 392 888 1384 268 1917
Fragments arranged by size:
1917 1384 888 876 392 268 15
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5543
AflIII A'CryG_T
Cuts at: 1203 4639 5616 1203
Size: 3436 977 1327
Fragments arranged by size:
3436 1327 977
AgeI A'CCGG_T
Cuts at: 2948 4571 2948
Size: 1623 4117
AhdI GACnn_n'nnGTC
Cuts at: 4469 4469
Size: 5740
AleI CACnn'nnGTG
Cuts at: 1166 2981 1166
Size: 1815 3925
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5740
AlwNI CAG_nnn'CTG
Cuts at: 2513 3483 5055 2513
Size: 970 1572 3198
Fragments arranged by size:
3198 1572 970
ApaLI G'TGCA_C
Cuts at: 3797 4953 3797
Size: 1156 4584
ApoI r'AATT_y
Cuts at: 1493 2090 3049 3275 3427 5536 1493
Size: 597 959 226 152 2109 1697
Fragments arranged by size:
2109 1697 959 597 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4294 160
Size: 872 3262 1606
Fragments arranged by size:
3262 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5740
AvaI C'yCGr_G
Cuts at: 1870 1943 3065 1870
Size: 73 1122 4545
Fragments arranged by size:
4545 1122 73
BamHI G'GATC_C
Cuts at: 3070 3437 3070
Size: 367 5373
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3061 4516 618
Size: 325 217 627 1274 1455 1842
Fragments arranged by size:
1842 1455 1274 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3059 1391
Size: 1668 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2301 2601 928
Size: 971 402 300 4067
Fragments arranged by size:
4067 971 402 300
BbvCI CC'TCA_GC
Cuts at: 2136 2136
Size: 5740
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3931 3965 1903
Size: 34 1994 34 3678
Fragments arranged by size:
3678 1994 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2253 3632 4842 2253
Size: 1379 1210 3151
Fragments arranged by size:
3151 1379 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5740
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2662 4351 136
Size: 107 122 71 631 605 990 1689 1525
Fragments arranged by size:
1689 1525 990 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2644 5735 2644
Size: 3091 2649
BlpI GC'TnA_GC
Cuts at: 3157 3157
Size: 5740
Bme1580I G_kGCm'C
Cuts at: 1790 3801 4957 1790
Size: 2011 1156 2573
Fragments arranged by size:
2573 2011 1156
BmgBI CAC'GTC
Cuts at: 1318 2983 1318
Size: 1665 4075
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2340 3471 4429
Size: 416 53 83 399 24 923 1131 958
Cuts at: 4429 442
Size: 1753
Fragments arranged by size:
1753 1131 958 923 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5740
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3011 4400 1239
Size: 1772 1389 2579
Fragments arranged by size:
2579 1772 1389
Bpu10I CC'TnA_GC
Cuts at: 2136 2136
Size: 5740
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3865 4730 5028 3865
Size: 865 298 4577
Fragments arranged by size:
4577 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2932 4403 882
Size: 617 1433 1471 2219
Fragments arranged by size:
2219 1471 1433 617
BsaAI yAC'GTr
Cuts at: 493 1204 3531 493
Size: 711 2327 2702
Fragments arranged by size:
2702 2327 711
BsaBI GATnn'nnATC
Cuts at: 2437 5734 2437
Size: 3297 2443
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3547 3929 4564 275
Size: 53 83 186 936 2014 382 635 1451
Fragments arranged by size:
2014 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2948 4173 4571 4845 4992 5403 2948
Size: 1225 398 274 147 411 3285
Fragments arranged by size:
3285 1225 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5515
Fragments arranged by size:
5515 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2747 3017 3051 1186
Size: 1561 270 34 3875
Fragments arranged by size:
3875 1561 270 34
BseYI C'CCAG_C
Cuts at: 4943 5439 4943
Size: 496 5244
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2737 3040 1163
Size: 573 1001 303 3863
Fragments arranged by size:
3863 1001 573 303
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3190 3951 4979 1540
Size: 275 186 1189 761 1028 2301
Fragments arranged by size:
2301 1189 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2997 3032 3801 3886 4957 2997
Size: 35 769 85 1071 3780
Fragments arranged by size:
3780 1071 769 85 35
BsmI GAATG_Cn'
Cuts at: 3251 3344 3251
Size: 93 5647
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2997 3032 3059 3801 3886 4957 1391
Size: 399 1207 35 27 742 85 1071 2174
Fragments arranged by size:
2174 1207 1071 742 399 85 35 27
BspEI T'CCGG_A
Cuts at: 5403 5403
Size: 5740
BspHI T'CATG_A
Cuts at: 3629 5359 3629
Size: 1730 4010
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2565 3095 844
Size: 1721 530 3489
Fragments arranged by size:
3489 1721 530
BsrBI CCG'CTC
Cuts at: 1394 1823 3627 1394
Size: 429 1804 3507
Fragments arranged by size:
3507 1804 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2884 4235 4409 65
Size: 1183 1636 1351 174 1396
Fragments arranged by size:
1636 1396 1351 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2045 2948 4384 4571 1700
Size: 110 235 903 1436 187 2869
Fragments arranged by size:
2869 1436 903 235 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5740
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5740
BssSI C'ACGA_G
Cuts at: 2921 3800 4812 2921
Size: 879 1012 3849
Fragments arranged by size:
3849 1012 879
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5740
BstBI TT'CG_AA
Cuts at: 4544 4544
Size: 5740
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5740
BstXI CCAn_nnnn'nTGG
Cuts at: 2625 2710 2625
Size: 85 5655
BstYI r'GATC_y
Cuts at: 1972 2131 2644 2713 3070 3437 3822 3839 5291
Size: 159 513 69 357 367 385 17 1452
Cuts at: 5291 5377 5389 5735 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 513 385 367 357 346 159
86 69 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5188
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5001
Fragments arranged by size:
5001 683 56
BtsI GCAGTG_nn'
Cuts at: 3256 4050 4070 3256
Size: 794 20 4926
Fragments arranged by size:
4926 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5740
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5705
DraI TTT'AAA
Cuts at: 3044 3044
Size: 5740
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4747 809
Size: 507 3431 1802
Fragments arranged by size:
3431 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2047 2816
Size: 54 1425 50 275 18 213 4 769
Cuts at: 2816 3187 4076 5705 8
Size: 371 889 1629 43
Fragments arranged by size:
1629 1425 889 769 371 275 213 54
50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 3187 1537
Size: 275 1375 4090
Fragments arranged by size:
4090 1375 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2018 3670 1353
Size: 665 1652 3423
Fragments arranged by size:
3423 1652 665
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2084 4327 4701 4847 387
Size: 1064 138 495 2243 374 146 1280
Fragments arranged by size:
2243 1280 1064 495 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2508 2523 2915 3011 3803 4400 5187
Size: 393 876 15 392 96 792 597 787
Cuts at: 5187 5455 1239
Size: 268 1524
Fragments arranged by size:
1524 876 792 787 597 393 392 268
96 15
EcoO109I rG'GnC_Cy
Cuts at: 2926 2926
Size: 5740
EcoRI G'AATT_C
Cuts at: 3049 3427 3049
Size: 378 5362
EcoRV GAT'ATC
Cuts at: 2609 2609
Size: 5740
FseI GG_CCGG'CC
Cuts at: 2049 2049
Size: 5740
FspI TGC'GCA
Cuts at: 4246 4246
Size: 5740
HaeII r_GCGC'y
Cuts at: 1678 2371 4887 1678
Size: 693 2516 2531
Fragments arranged by size:
2531 2516 693
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4381 4413 4455 4487 1373
Size: 32 135 32 2809 32 42 32 2626
Fragments arranged by size:
2809 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3084 3330 669
Size: 988 281 1146 246 3079
Fragments arranged by size:
3079 1146 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5740
HpaI GTT'AAC
Cuts at: 3330 3330
Size: 5740
KpnI G_GTAC'C
Cuts at: 3065 3065
Size: 5740
MfeI C'AATT_G
Cuts at: 2872 3339 2872
Size: 467 5273
MluI A'CGCG_T
Cuts at: 5616 5616
Size: 5740
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2100 2613 4854 5038 1305
Size: 795 513 2241 184 2007
Fragments arranged by size:
2241 2007 795 513 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2818 10
Size: 54 1425 343 986 2932
Fragments arranged by size:
2932 1425 986 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2492 2981 3698 4057 4216 518
Size: 648 1326 489 717 359 159 2042
Fragments arranged by size:
2042 1326 717 648 489 359 159
MspA1I CmG'CkG
Cuts at: 1606 2751 3156 3833 4981 5226 1606
Size: 1145 405 677 1148 245 2120
Fragments arranged by size:
2120 1148 1145 677 405 245
NaeI GCC'GGC
Cuts at: 2047 2047
Size: 5740
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5188
NdeI CA'TA_TG
Cuts at: 387 2312 387
Size: 1925 3815
NgoMIV G'CCGG_C
Cuts at: 2045 2045
Size: 5740
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5740
NotI GC'GGCC_GC
Cuts at: 3187 3187
Size: 5740
NspI r_CATG'y
Cuts at: 1132 1229 3098 4643 1132
Size: 97 1869 1545 2229
Fragments arranged by size:
2229 1869 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5740
PciI A'CATG_T
Cuts at: 4639 4639
Size: 5740
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2814 1108
Size: 1706 4034
PmeI GTTT'AAAC
Cuts at: 3044 3044
Size: 5740
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3809 3841 5322 5354 3809
Size: 32 1481 32 4195
Fragments arranged by size:
4195 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2926 2926
Size: 5740
PshAI GACnn'nnGTC
Cuts at: 1613 2951 1613
Size: 1338 4402
PsiI TTA'TAA
Cuts at: 3310 3310
Size: 5740
PstI C_TGCA'G
Cuts at: 830 3092 830
Size: 2262 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5740
PvuII CAG'CTG
Cuts at: 2751 2751
Size: 5740
SalI G'TCGA_C
Cuts at: 1936 3082 1936
Size: 1146 4594
SbfI CC_TGCA'GG
Cuts at: 3092 3092
Size: 5740
ScaI AGT'ACT
Cuts at: 3988 5700 3988
Size: 1712 4028
SfcI C'TryA_G
Cuts at: 826 1045 3088 4223 4904 5095 826
Size: 219 2043 1135 681 191 1471
Fragments arranged by size:
2043 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5740
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5740
SmaI CCC'GGG
Cuts at: 1872 3067 1872
Size: 1195 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3844 4745 5007 820
Size: 197 926 1901 901 262 1553
Fragments arranged by size:
1901 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3531 493
Size: 3038 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5740
SphI G_CATG'C
Cuts at: 1132 3098 1132
Size: 1966 3774
SspI AAT'ATT
Cuts at: 5 52 2450 3664 5
Size: 47 2398 1214 2081
Fragments arranged by size:
2398 2081 1214 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5740
StyI C'CwwG_G
Cuts at: 513 1065 2186 2589 3179 513
Size: 552 1121 403 590 3074
Fragments arranged by size:
3074 1121 590 552 403
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3767 3784 3937 4122 4567 325
Size: 1255 2187 17 153 185 445 1498
Fragments arranged by size:
2187 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2125 3986 5698
Size: 274 80 33 51 1443 148 1861 1712
Cuts at: 5698 96
Size: 138
Fragments arranged by size:
1861 1712 1443 274 148 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5700 5732 5700
Size: 32 5708
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5740
XbaI T'CTAG_A
Cuts at: 3076 3076
Size: 5740
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5740
XmaI C'CCGG_G
Cuts at: 1870 3065 1870
Size: 1195 4545
XmnI GAAnn'nnTTC
Cuts at: 2451 3869 2451
Size: 1418 4322
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3548 276
Size: 53 83 186 936 2014 2468
Fragments arranged by size:
2468 2014 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FseI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI FalI FspAI KasI NarI NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI