Restriction map for
pFN21AE1824
(Circular) (Six-base) MAPSORT of: pFN21AE1824.seq Check: 2775 from: 1 to: 5728
pFN21AE1824 5728 bp
With 182 enzymes: SgfI *
June 20, 2019 11:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3538 278
Size: 53 83 186 936 2002 2468
Fragments arranged by size:
2468 2002 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3071 1937
Size: 1134 4594
Acc65I G'GTAC_C
Cuts at: 2542 3049 2542
Size: 507 5221
AclI AA'CG_TT
Cuts at: 1563 3027 3855 4228 1563
Size: 1464 828 373 3063
Fragments arranged by size:
3063 1464 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2591 3791 5175 5443 1632
Size: 959 1200 1384 268 1917
Fragments arranged by size:
1917 1384 1200 959 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5531
AflIII A'CryG_T
Cuts at: 1203 2169 2458 4627 5604 1203
Size: 966 289 2169 977 1327
Fragments arranged by size:
2169 1327 977 966 289
AgeI A'CCGG_T
Cuts at: 4559 4559
Size: 5728
AhdI GACnn_n'nnGTC
Cuts at: 4457 4457
Size: 5728
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5728
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5728
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2226 2258 2226
Size: 32 5696
AlwNI CAG_nnn'CTG
Cuts at: 3471 5043 3471
Size: 1572 4156
ApaI G_GGCC'C
Cuts at: 2841 2841
Size: 5728
ApaLI G'TGCA_C
Cuts at: 3785 4941 3785
Size: 1156 4572
ApoI r'AATT_y
Cuts at: 1493 3037 3263 3415 5524 1493
Size: 1544 226 152 2109 1697
Fragments arranged by size:
2109 1697 1544 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4282 160
Size: 872 3250 1606
Fragments arranged by size:
3250 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5728
AvaI C'yCGr_G
Cuts at: 1870 1943 2126 3053 1870
Size: 73 183 927 4545
Fragments arranged by size:
4545 927 183 73
BamHI G'GATC_C
Cuts at: 3058 3425 3058
Size: 367 5361
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2542 2609 2887 3049 4504
Size: 325 217 627 755 67 278 162 1455
Cuts at: 4504 618
Size: 1842
Fragments arranged by size:
1842 1455 755 627 325 278 217 162
67
BanII G_rGCy'C
Cuts at: 1391 2841 3047 1391
Size: 1450 206 4072
Fragments arranged by size:
4072 1450 206
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2568 928
Size: 971 669 4088
Fragments arranged by size:
4088 971 669
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3919 3953 1903
Size: 34 1982 34 3678
Fragments arranged by size:
3678 1982 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2922 3620 4830 2922
Size: 698 1210 3820
Fragments arranged by size:
3820 1210 698
BclI T'GATC_A
Cuts at: 1548 2766 1548
Size: 1218 4510
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4339 136
Size: 107 122 71 631 605 2667 1525
Fragments arranged by size:
2667 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5723 5723
Size: 5728
BlpI GC'TnA_GC
Cuts at: 3145 3145
Size: 5728
Bme1580I G_kGCm'C
Cuts at: 1790 2841 3789 4945 1790
Size: 1051 948 1156 2573
Fragments arranged by size:
2573 1156 1051 948
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5728
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2703 3459 4417
Size: 416 53 83 399 24 1286 756 958
Cuts at: 4417 442
Size: 1753
Fragments arranged by size:
1753 1286 958 756 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5728
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2817 4388 1239
Size: 1578 1571 2579
Fragments arranged by size:
2579 1578 1571
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2927 3853 4718 5016 2927
Size: 926 865 298 3639
Fragments arranged by size:
3639 926 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4391 882
Size: 617 2892 2219
Fragments arranged by size:
2892 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3519 493
Size: 711 2315 2702
Fragments arranged by size:
2702 2315 711
BsaBI GATnn'nnATC
Cuts at: 5722 5722
Size: 5728
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3535 3917 4552 275
Size: 53 83 186 936 2002 382 635 1451
Fragments arranged by size:
2002 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2354 4161 4559 4833 4980 5391 2354
Size: 1807 398 274 147 411 2691
Fragments arranged by size:
2691 1807 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5503
Fragments arranged by size:
5503 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2423 2796 1186
Size: 1237 373 4118
Fragments arranged by size:
4118 1237 373
BseYI C'CCAG_C
Cuts at: 4931 5427 4931
Size: 496 5232
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2232 1163
Size: 573 496 4659
Fragments arranged by size:
4659 573 496
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3178 3939 4967 1540
Size: 275 186 1177 761 1028 2301
Fragments arranged by size:
2301 1177 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3789 3874 4945 3789
Size: 85 1071 4572
Fragments arranged by size:
4572 1071 85
BsmI GAATG_Cn'
Cuts at: 3239 3332 3239
Size: 93 5635
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2841 3047 3789 3874 4945 1391
Size: 399 1051 206 742 85 1071 2174
Fragments arranged by size:
2174 1071 1051 742 399 206 85
BspEI T'CCGG_A
Cuts at: 5391 5391
Size: 5728
BspHI T'CATG_A
Cuts at: 2085 3617 5347 2085
Size: 1532 1730 2466
Fragments arranged by size:
2466 1730 1532
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2554 3083 844
Size: 1710 529 3489
Fragments arranged by size:
3489 1710 529
BsrBI CCG'CTC
Cuts at: 1394 1823 2131 3615 1394
Size: 429 308 1484 3507
Fragments arranged by size:
3507 1484 429 308
BsrDI GCAATG_nn'
Cuts at: 65 1248 4223 4397 65
Size: 1183 2975 174 1396
Fragments arranged by size:
2975 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2704 3020 4372 4559 1700
Size: 110 894 316 1352 187 2869
Fragments arranged by size:
2869 1352 894 316 187 110
BsrGI T'GTAC_A
Cuts at: 96 2172 96
Size: 2076 3652
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5728
BssSI C'ACGA_G
Cuts at: 3788 4800 3788
Size: 1012 4716
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2971 794
Size: 2177 3551
BstBI TT'CG_AA
Cuts at: 2959 4532 2959
Size: 1573 4155
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5728
BstYI r'GATC_y
Cuts at: 1972 2112 3058 3425 3810 3827 5279 5365 5377
Size: 140 946 367 385 17 1452 86 12
Cuts at: 5377 5723 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 946 385 367 346 140 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4237
Fragments arranged by size:
4237 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4989
Fragments arranged by size:
4989 683 56
BtsI GCAGTG_nn'
Cuts at: 3244 4038 4058 3244
Size: 794 20 4914
Fragments arranged by size:
4914 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5728
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5693
DraI TTT'AAA
Cuts at: 3032 3032
Size: 5728
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4735 809
Size: 507 3419 1802
Fragments arranged by size:
3419 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3175 4064 5693
Size: 54 1425 50 275 18 1345 889 1629
Cuts at: 5693 8
Size: 43
Fragments arranged by size:
1629 1425 1345 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3175 1537
Size: 275 1363 4090
Fragments arranged by size:
4090 1363 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2504 3658 1353
Size: 1151 1154 3423
Fragments arranged by size:
3423 1154 1151
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4315 4689 4835 387
Size: 1064 138 2726 374 146 1280
Fragments arranged by size:
2726 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2591 2817 3791 4388 5175 5443 1239
Size: 393 959 226 974 597 787 268 1524
Fragments arranged by size:
1524 974 959 787 597 393 268 226
EcoO109I rG'GnC_Cy
Cuts at: 2234 2837 2234
Size: 603 5125
EcoRI G'AATT_C
Cuts at: 3037 3415 3037
Size: 378 5350
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2276 2276
Size: 5728
FspI TGC'GCA
Cuts at: 4234 4234
Size: 5728
HaeII r_GCGC'y
Cuts at: 1678 4875 1678
Size: 3197 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2349 2381 2484 2516 4369
Size: 32 135 32 777 32 103 32 1853
Cuts at: 4369 4401 4443 4475 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1853 777 135 103 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3072 3318 669
Size: 988 281 1134 246 3079
Fragments arranged by size:
3079 1134 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5728
HpaI GTT'AAC
Cuts at: 3318 3318
Size: 5728
KpnI G_GTAC'C
Cuts at: 2546 3053 2546
Size: 507 5221
MfeI C'AATT_G
Cuts at: 2431 3327 2431
Size: 896 4832
MluI A'CGCG_T
Cuts at: 5604 5604
Size: 5728
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4842 5026 1305
Size: 3537 184 2007
Fragments arranged by size:
3537 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3906
Fragments arranged by size:
3906 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2445 3686 4045 4204 518
Size: 648 1279 1241 359 159 2042
Fragments arranged by size:
2042 1279 1241 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2065 2584 2720 2744 2786 3144 3821 4969
Size: 459 519 136 24 42 358 677 1148
Cuts at: 4969 5214 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 519 459 358 245 136
42 24
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4237
Fragments arranged by size:
4237 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5728
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5728
NotI GC'GGCC_GC
Cuts at: 3175 3175
Size: 5728
NspI r_CATG'y
Cuts at: 1132 1229 2173 2462 3086 4631 1132
Size: 97 944 289 624 1545 2229
Fragments arranged by size:
2229 1545 944 624 289 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5728
PciI A'CATG_T
Cuts at: 2169 2458 4627 2169
Size: 289 2169 3270
Fragments arranged by size:
3270 2169 289
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5728
PmeI GTTT'AAAC
Cuts at: 3032 3032
Size: 5728
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3797 3829 5310 5342 3797
Size: 32 1481 32 4183
Fragments arranged by size:
4183 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2234 2234
Size: 5728
PshAI GACnn'nnGTC
Cuts at: 1613 2786 1613
Size: 1173 4555
PsiI TTA'TAA
Cuts at: 3298 3298
Size: 5728
PspOMI G'GGCC_C
Cuts at: 2837 2837
Size: 5728
PspXI vC'TCGA_Gb
Cuts at: 2126 2126
Size: 5728
PstI C_TGCA'G
Cuts at: 830 2551 3080 830
Size: 1721 529 3478
Fragments arranged by size:
3478 1721 529
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5728
PvuII CAG'CTG
Cuts at: 2065 2584 2720 2744 2065
Size: 519 136 24 5049
Fragments arranged by size:
5049 519 136 24
SalI G'TCGA_C
Cuts at: 1936 3070 1936
Size: 1134 4594
SbfI CC_TGCA'GG
Cuts at: 3080 3080
Size: 5728
ScaI AGT'ACT
Cuts at: 3976 5688 3976
Size: 1712 4016
SfcI C'TryA_G
Cuts at: 826 1045 2547 2614 3076 4211 4892 5083 826
Size: 219 1502 67 462 1135 681 191 1471
Fragments arranged by size:
1502 1471 1135 681 462 219 191 67
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5728
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5728
SmaI CCC'GGG
Cuts at: 1872 3055 1872
Size: 1183 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2126 2906 3832 4733 4995 820
Size: 197 926 183 780 926 901 262 1553
Fragments arranged by size:
1553 926 926 901 780 262 197 183
SnaBI TAC'GTA
Cuts at: 493 3519 493
Size: 3026 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5728
SphI G_CATG'C
Cuts at: 1132 3086 1132
Size: 1954 3774
SspI AAT'ATT
Cuts at: 5 52 3652 5
Size: 47 3600 2081
Fragments arranged by size:
3600 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5728
StyI C'CwwG_G
Cuts at: 513 1065 2004 2311 2681 3167 513
Size: 552 939 307 370 486 3074
Fragments arranged by size:
3074 939 552 486 370 307
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2826 3024 3755 3772 3925 4110 4555
Size: 1255 1246 198 731 17 153 185 445
Cuts at: 4555 325
Size: 1498
Fragments arranged by size:
1498 1255 1246 731 445 198 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2172 2195 2461
Size: 274 80 33 51 1443 195 23 266
Cuts at: 2461 3974 5686 96
Size: 1513 1712 138
Fragments arranged by size:
1712 1513 1443 274 266 195 138 80
51 33 23
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5688 5720 5688
Size: 32 5696
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5728
XbaI T'CTAG_A
Cuts at: 3064 3064
Size: 5728
XhoI C'TCGA_G
Cuts at: 1943 2126 1943
Size: 183 5545
XmaI C'CCGG_G
Cuts at: 1870 3053 1870
Size: 1183 4545
XmnI GAAnn'nnTTC
Cuts at: 3857 3857
Size: 5728
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3536 276
Size: 53 83 186 936 2002 2468
Fragments arranged by size:
2468 2002 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI