Restriction map for
pFN21AE1823
(Circular) (Six-base) MAPSORT of: pFN21AE1823.seq Check: 6011 from: 1 to: 5713
pFN21AE1823 5713 bp
With 182 enzymes: SgfI *
July 19, 2019 13:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3523 278
Size: 53 83 186 936 1987 2468
Fragments arranged by size:
2468 1987 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2043 3056 1937
Size: 106 1013 4594
Fragments arranged by size:
4594 1013 106
Acc65I G'GTAC_C
Cuts at: 3034 3034
Size: 5713
AclI AA'CG_TT
Cuts at: 1563 3840 4213 1563
Size: 2277 373 3063
Fragments arranged by size:
3063 2277 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2322 3776 5160 5428 1632
Size: 690 1454 1384 268 1917
Fragments arranged by size:
1917 1454 1384 690 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5516
AflIII A'CryG_T
Cuts at: 1203 2100 4612 5589 1203
Size: 897 2512 977 1327
Fragments arranged by size:
2512 1327 977 897
AgeI A'CCGG_T
Cuts at: 2921 4544 2921
Size: 1623 4090
AhdI GACnn_n'nnGTC
Cuts at: 4442 4442
Size: 5713
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5713
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5713
AlwNI CAG_nnn'CTG
Cuts at: 3456 5028 3456
Size: 1572 4141
ApaLI G'TGCA_C
Cuts at: 3770 4926 3770
Size: 1156 4557
ApoI r'AATT_y
Cuts at: 1493 2251 2560 3022 3248 3400 5509 1493
Size: 758 309 462 226 152 2109 1697
Fragments arranged by size:
2109 1697 758 462 309 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4267 160
Size: 872 3235 1606
Fragments arranged by size:
3235 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5713
AvaI C'yCGr_G
Cuts at: 1870 1943 2653 3038 1870
Size: 73 710 385 4545
Fragments arranged by size:
4545 710 385 73
BamHI G'GATC_C
Cuts at: 3043 3410 3043
Size: 367 5346
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2890 3034 4489 618
Size: 325 217 627 1103 144 1455 1842
Fragments arranged by size:
1842 1455 1103 627 325 217 144
BanII G_rGCy'C
Cuts at: 1391 2053 3032 1391
Size: 662 979 4072
Fragments arranged by size:
4072 979 662
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4742
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3904 3938 1903
Size: 34 1967 34 3678
Fragments arranged by size:
3678 1967 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2130 2872 3605 4815 2130
Size: 742 733 1210 3028
Fragments arranged by size:
3028 1210 742 733
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5713
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4324 136
Size: 107 122 71 631 605 2652 1525
Fragments arranged by size:
2652 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2857 5708 2857
Size: 2851 2862
BlpI GC'TnA_GC
Cuts at: 3130 3130
Size: 5713
Bme1580I G_kGCm'C
Cuts at: 1790 3774 4930 1790
Size: 1984 1156 2573
Fragments arranged by size:
2573 1984 1156
BmgBI CAC'GTC
Cuts at: 1318 2475 1318
Size: 1157 4556
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3444 4402 442
Size: 416 53 83 399 24 2027 958 1753
Fragments arranged by size:
2027 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5713
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2787 4373 1239
Size: 1548 1586 2579
Fragments arranged by size:
2579 1586 1548
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3838 4703 5001 3838
Size: 865 298 4550
Fragments arranged by size:
4550 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4376 882
Size: 617 2877 2219
Fragments arranged by size:
2877 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3504 493
Size: 711 2300 2702
Fragments arranged by size:
2702 2300 711
BsaBI GATnn'nnATC
Cuts at: 5707 5707
Size: 5713
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3520 3902 4537 275
Size: 53 83 186 936 1987 382 635 1451
Fragments arranged by size:
1987 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2921 4146 4544 4818 4965 5376 2921
Size: 1225 398 274 147 411 3258
Fragments arranged by size:
3258 1225 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2760 2790 1178
Size: 30 165 30 1357 30 4101
Fragments arranged by size:
4101 1357 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5713
BseYI C'CCAG_C
Cuts at: 2169 4916 5412 2169
Size: 2747 496 2470
Fragments arranged by size:
2747 2470 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2504 1163
Size: 573 768 4372
Fragments arranged by size:
4372 768 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3163 3924 4952 1540
Size: 275 186 1162 761 1028 2301
Fragments arranged by size:
2301 1162 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2053 3774 3859 4930 2053
Size: 1721 85 1071 2836
Fragments arranged by size:
2836 1721 1071 85
BsmI GAATG_Cn'
Cuts at: 2299 3224 3317 2299
Size: 925 93 4695
Fragments arranged by size:
4695 925 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2053 3032 3774 3859 4930 1391
Size: 399 263 979 742 85 1071 2174
Fragments arranged by size:
2174 1071 979 742 399 263 85
BspEI T'CCGG_A
Cuts at: 5376 5376
Size: 5713
BspHI T'CATG_A
Cuts at: 3602 5332 3602
Size: 1730 3983
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3068 844
Size: 2224 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3600 1394
Size: 429 1777 3507
Fragments arranged by size:
3507 1777 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4208 4382 65
Size: 1183 2960 174 1396
Fragments arranged by size:
2960 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2921 4357 4544 1700
Size: 110 1111 1436 187 2869
Fragments arranged by size:
2869 1436 1111 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5713
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5713
BssSI C'ACGA_G
Cuts at: 3773 4785 3773
Size: 1012 4701
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5713
BstBI TT'CG_AA
Cuts at: 4517 4517
Size: 5713
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5713
BstYI r'GATC_y
Cuts at: 1972 2505 2857 3043 3410 3795 3812 5264 5350
Size: 533 352 186 367 385 17 1452 86
Cuts at: 5350 5362 5708 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 533 385 367 352 346 186
86 17 12
BstZ17I GTA'TAC
Cuts at: 2044 2044
Size: 5713
BtgI C'CryG_G
Cuts at: 513 1065 2004 2551 2605 2943 513
Size: 552 939 547 54 338 3283
Fragments arranged by size:
3283 939 552 547 338 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4974
Fragments arranged by size:
4974 683 56
BtsI GCAGTG_nn'
Cuts at: 2496 3229 4023 4043 2496
Size: 733 794 20 4166
Fragments arranged by size:
4166 794 733 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5713
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2797 2832 529
Size: 35 2233 35 3410
Fragments arranged by size:
3410 2233 35 35
DraI TTT'AAA
Cuts at: 3017 3017
Size: 5713
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4720 809
Size: 507 3404 1802
Fragments arranged by size:
3404 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3160 4049 5678
Size: 54 1425 50 275 18 1330 889 1629
Cuts at: 5678 8
Size: 43
Fragments arranged by size:
1629 1425 1330 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3160 1537
Size: 275 1348 4090
Fragments arranged by size:
4090 1348 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2037 2055 2848 3643 1353
Size: 684 18 793 795 3423
Fragments arranged by size:
3423 795 793 684 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4300 4674 4820 387
Size: 1064 138 2711 374 146 1280
Fragments arranged by size:
2711 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2051 2051
Size: 5713
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2322 2787 3776 4373 5160 5428 1239
Size: 393 690 465 989 597 787 268 1524
Fragments arranged by size:
1524 989 787 690 597 465 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2166 2166
Size: 5713
EcoRI G'AATT_C
Cuts at: 2251 3022 3400 2251
Size: 771 378 4564
Fragments arranged by size:
4564 771 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2084 2084
Size: 5713
FspI TGC'GCA
Cuts at: 4219 4219
Size: 5713
HaeII r_GCGC'y
Cuts at: 1678 4860 1678
Size: 3182 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2087 2119 2842 2874 4354
Size: 32 135 32 515 32 723 32 1480
Cuts at: 4354 4386 4428 4460 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1480 723 515 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2927 2974 3057 3303 669
Size: 988 281 989 47 83 246 3079
Fragments arranged by size:
3079 989 988 281 246 83 47
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5713
HpaI GTT'AAC
Cuts at: 3303 3303
Size: 5713
KpnI G_GTAC'C
Cuts at: 3038 3038
Size: 5713
MfeI C'AATT_G
Cuts at: 3312 3312
Size: 5713
MluI A'CGCG_T
Cuts at: 5589 5589
Size: 5713
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3005 4827 5011 1305
Size: 1700 1822 184 2007
Fragments arranged by size:
2007 1822 1700 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3891
Fragments arranged by size:
3891 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3671 4030 4189 518
Size: 648 2505 359 159 2042
Fragments arranged by size:
2505 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2402 2667 3129 3806 4954 5199 1606
Size: 796 265 462 677 1148 245 2120
Fragments arranged by size:
2120 1148 796 677 462 265 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2551 2943 513
Size: 552 939 547 392 3283
Fragments arranged by size:
3283 939 552 547 392
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5713
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5713
NotI GC'GGCC_GC
Cuts at: 3160 3160
Size: 5713
NspI r_CATG'y
Cuts at: 1132 1229 2104 3071 4616 1132
Size: 97 875 967 1545 2229
Fragments arranged by size:
2229 1545 967 875 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5713
PciI A'CATG_T
Cuts at: 2100 4612 2100
Size: 2512 3201
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5713
PmeI GTTT'AAAC
Cuts at: 3017 3017
Size: 5713
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3782 3814 5295 5327 3782
Size: 32 1481 32 4168
Fragments arranged by size:
4168 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2166 2166
Size: 5713
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5713
PsiI TTA'TAA
Cuts at: 3283 3283
Size: 5713
PstI C_TGCA'G
Cuts at: 830 2603 3065 830
Size: 1773 462 3478
Fragments arranged by size:
3478 1773 462
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5713
PvuII CAG'CTG
Cuts at: 2402 2402
Size: 5713
SacI G_AGCT'C
Cuts at: 2053 2053
Size: 5713
SalI G'TCGA_C
Cuts at: 1936 3055 1936
Size: 1119 4594
SanDI GG'GwC_CC
Cuts at: 2166 2166
Size: 5713
SapI GCTCTTCn'nnn_
Cuts at: 2037 2037
Size: 5713
SbfI CC_TGCA'GG
Cuts at: 3065 3065
Size: 5713
ScaI AGT'ACT
Cuts at: 3961 5673 3961
Size: 1712 4001
SfcI C'TryA_G
Cuts at: 826 1045 2599 2721 2902 3061 4196 4877 5068
Size: 219 1554 122 181 159 1135 681 191
Cuts at: 5068 826
Size: 1471
Fragments arranged by size:
1554 1471 1135 681 219 191 181 159
122
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5713
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5713
SmaI CCC'GGG
Cuts at: 1872 3040 1872
Size: 1168 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3817 4718 4980 820
Size: 197 926 1874 901 262 1553
Fragments arranged by size:
1874 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3504 493
Size: 3011 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5713
SphI G_CATG'C
Cuts at: 1132 3071 1132
Size: 1939 3774
SspI AAT'ATT
Cuts at: 5 52 3637 5
Size: 47 3585 2081
Fragments arranged by size:
3585 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5713
StyI C'CwwG_G
Cuts at: 513 1065 2004 2551 2943 3152 513
Size: 552 939 547 392 209 3074
Fragments arranged by size:
3074 939 552 547 392 209
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2908 3740 3757 3910 4095 4540 325
Size: 1255 1328 832 17 153 185 445 1498
Fragments arranged by size:
1498 1328 1255 832 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3959 5671 96
Size: 274 80 33 51 1443 1982 1712 138
Fragments arranged by size:
1982 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5673 5705 5673
Size: 32 5681
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5713
XbaI T'CTAG_A
Cuts at: 2107 3049 2107
Size: 942 4771
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2352 2941 2352
Size: 589 5124
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5713
XmaI C'CCGG_G
Cuts at: 1870 3038 1870
Size: 1168 4545
XmnI GAAnn'nnTTC
Cuts at: 3842 3842
Size: 5713
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3521 276
Size: 53 83 186 936 1987 2468
Fragments arranged by size:
2468 1987 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I
DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI